Team:UCLA/Parts

From 2013.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
+
{{:Team:UCLA/Template}}
-
<style>
+
==Mtd (BPP-1 bacteriophage)==
-
table.pos_left
+
This part codes for the Major Tropism Determinant (Mtd) protein responsible for selective protein binding by the BPP-1 bacteriophage. In its native form, it has moderate affinity to the pertactin proteins expressed on the surface of Bordetella, the phage's natural host.
-
{
+
The mtd gene has 22 specific nucleotides in its sequence that can be mutagenized to change the binding properties without adversely affecting the folding and stability of the protein. Mutations to these nucleotides give the Mtd protein the potential to bind to a very large range of target proteins. The mutagenic sites are highlighted below in red:
-
position:relative;
+
-
left:-6px;
+
-
}
+
-
</style>
+
-
<table class="pos_left" cellspacing="0" cellpadding="0">
+
[[File:Mtd-mutagenic_sites.PNG|thumb|left|Mutagenic Sites on <i>mtd</i> gene.|300px]]
-
<tr>
 
-
<td bgcolor = "white">
 
-
<body>
 
-
<div id = "header">
 
-
<center><a href="https://2013.igem.org/Team:UCLA"><img src="http://www.synbioatucla.com/logo.jpg" width=450></a></center>
 
-
</div>
 
-
 
-
<div id = "menu">
 
-
<table cellspacing="0" cellpadding="0">
 
-
<tr>
 
-
<td><a href="https://2013.igem.org/Team:UCLA">
 
-
<img src="http://www.synbioatucla.com/home.jpg" name="home" width=100%></a></td>
 
-
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Team">
 
-
<img src="http://www.synbioatucla.com/team.jpg" name="team"  width=100%></a></td>
 
-
 
-
<td><a href="https://igem.org/Team.cgi?year=2013">
 
-
<img src="https://static.igem.org/mediawiki/2013/3/32/Team_profile.jpg" name="teamprofile"  width=100%></a></td>
 
-
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Project">
 
-
<img src="http://www.synbioatucla.com/project.jpg" name="project"  width=100%></a></td>
 
-
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Parts">
 
-
<img src="https://static.igem.org/mediawiki/2013/f/fc/Registryparts.jpg" name="parts"  width=100%></a></td>
 
-
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Modeling">
 
-
<img src="https://static.igem.org/mediawiki/2013/1/12/Modeling_ucla.jpg" name="modeling"  width=100%></a></td>
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Notebook">
 
-
<img src="https://static.igem.org/mediawiki/2013/d/db/Notebook_ucla.jpg" name="notebook"  width=100%></a></td>
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Safety">
 
-
<img src="https://static.igem.org/mediawiki/2013/c/c2/Safety_ucla.jpg" name="safety"  width=100%></a></td>
 
-
<td><a href="https://2013.igem.org/Team:UCLA/Attributions">
 
-
<img src="https://static.igem.org/mediawiki/2013/9/91/Attributions.jpg" name="attributions" width=100%></a></td>
 
-
</tr>
 
-
</table>
 
-
</div>
 
-
</body>
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
</html>
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
-
!align="center"|[[Team:UCLA|Home]]
 
-
!align="center"|[[Team:UCLA/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=UCLA Official Team Profile]
 
-
!align="center"|[[Team:UCLA/Project|Project]]
 
-
!align="center"|[[Team:UCLA/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:UCLA/Modeling|Modeling]]
 
-
!align="center"|[[Team:UCLA/Notebook|Notebook]]
 
-
!align="center"|[[Team:UCLA/Safety|Safety]]
 
-
!align="center"|[[Team:UCLA/Attributions|Attributions]]
 
-
|}
 
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
-
Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
+
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
In the natural phage system, libraries of mtd are created by the BPP-1 bacteriophage's Diversity Generating Retroelement. For <i>in vitro</i> library generation, synthetic methods such as PCR using variable oligonucleotides can be used.
<groupparts>iGEM013 UCLA</groupparts>
<groupparts>iGEM013 UCLA</groupparts>

Latest revision as of 01:54, 28 September 2013

Mtd (BPP-1 bacteriophage)

This part codes for the Major Tropism Determinant (Mtd) protein responsible for selective protein binding by the BPP-1 bacteriophage. In its native form, it has moderate affinity to the pertactin proteins expressed on the surface of Bordetella, the phage's natural host. The mtd gene has 22 specific nucleotides in its sequence that can be mutagenized to change the binding properties without adversely affecting the folding and stability of the protein. Mutations to these nucleotides give the Mtd protein the potential to bind to a very large range of target proteins. The mutagenic sites are highlighted below in red:

Mutagenic Sites on mtd gene.














In the natural phage system, libraries of mtd are created by the BPP-1 bacteriophage's Diversity Generating Retroelement. For in vitro library generation, synthetic methods such as PCR using variable oligonucleotides can be used.


<groupparts>iGEM013 UCLA</groupparts>

Retrieved from "http://2013.igem.org/Team:UCLA/Parts"