"
Team:DTU-Denmark/Methods
From 2013.igem.org
(Difference between revisions)
(→Plasmid isolation) |
(→Other) |
||
| (25 intermediate revisions not shown) | |||
| Line 17: | Line 17: | ||
[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]] | [[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]] | ||
| + | |||
| + | |||
| + | ==USER Cloning== | ||
| + | [[Team:DTU-Denmark/Methods/USER_Cloning_and_Transformation|USER Cloning and Transformation]] | ||
==Plasmid isolation== | ==Plasmid isolation== | ||
| Line 22: | Line 26: | ||
[[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]] | [[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]] | ||
| + | ==Determining concentration of nitrogen compounds== | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_1a|Experiment 1a]] -- Toxicity of Nitrite, Nitrate and Ammonium to ''E. coli'' | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Experiment 2]] -- Anaerobic production of nitrous oxide from nitrite | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_4|Experiment 4]] -- Aerobic consumption of ammonium and hydroxylamine | ||
| + | |||
| + | ==Other== | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Visualizing_GFP_in_the_periplasm|Visualizing GFP in the periplasm]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/chemically_compentent_cells|Preparation of chemically competent cells]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Autoclaving|Autoclaving]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Making anoxic conditions|Sparging with N<sub>2</sub>]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Modified DM minimal medium|Modified DM minimal medium]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Calibrating Electrodes|Calibration of Electrodes]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Ammonium colorimetric measurements|Ammonium colorimetric measurements]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Nitrate colorimetric measurements|Nitrate colorimetric measurements]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/Nitrite colorimetric measurements|Nitrite colorimetric measurements]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/rehydrating_primers|Rehydrating Primers]] | ||
| + | |||
| + | [[Team:DTU-Denmark/Methods/dpni_treatment|DpnI treatment]] | ||
| + | [[Team:DTU-Denmark/Methods/Bioreactor|Bioreactor]] | ||
| + | <div style="clear: both;"></div> | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 12:31, 1 October 2013
Methods
Contents |
PCR
USER Cloning
USER Cloning and Transformation
Plasmid isolation
Plasmid isolation by ethanol precipitation
Determining concentration of nitrogen compounds
Experiment 1a -- Toxicity of Nitrite, Nitrate and Ammonium to E. coli
Experiment 2 -- Anaerobic production of nitrous oxide from nitrite
Experiment 4 -- Aerobic consumption of ammonium and hydroxylamine
Other
Visualizing GFP in the periplasm
Preparation of chemically competent cells
Ammonium colorimetric measurements
Nitrate colorimetric measurements
Nitrite colorimetric measurements
