Small Phage

From 2013.igem.org

(Difference between revisions)

Latest revision as of 15:06, 24 May 2013

Small Phage (Add formatting)

Overview

Winter

Spring

Summer

Fall

add overview

Description of our purpose and approaches

Pages that needs deleting

Spring Winter

Things to figure out

How to format table?

How to format text besides default?

Domain/URL?

text alignment in table

add picture

March

April

May

5/1/13

- Commencement of spring!!!

- Discussed goals and outlined plans for spring term

5/2/13

- Designed primers for amplifying and sequencing phage capsid protein

- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly

5/3/13

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification

5/4/13

- Performed dilution series using stock 5.3 (-1 through -11)

- Started two 5mL overnights of BL21

5/5/13

- Spot test using stock 5.3 and its dilution series (from 5.4)

5.3 T7 phage amplification/purification

- Started liquid culture for purification team (at around noon)

1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)

- Started 5.5 amplification from a plaque test

5.5 Amplification from a plaque test

5/6/13

- Continued 5.3 T7 phage amplification/purification

- Performed liquid culture phage concentration test

5.6 T7+ Liquid Culture Phage Concentration Test

5/7/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/8/13

- Went over procedure for applying mutagen and PCR with Dr. Grose

- Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test

- Started two 5mL BL21 overnights

- Learned how to create LB plates

5/9/13

- Practiced with ×6 and ×8 top agar

5.9 T7 selection method test #2

- Started 5.9 T7+ Liquid Culture Phage Concentration Test #2

- Discussed plans and schedule for next week.

5/20/13

LP, XL

- We performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- We performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR

5/21/13

XL

- started two 5mL E coli BL21 overnight at around 7:00pm

5/22/13

- Continued with T7 Mutagen Concentration Test

- Continued with T7 Minor Capsid Protein PCR by running an agarose gel to confirm that we got the desired PCR product.

@@ Line 1: / Line 1: @@
 {{TeamBYUProvo}}
-==March==
+{| width="100%"
-===3/15/13===
+| colspan="3" | Small Phage (Add formatting)
-===3/18/13===
+|-
+| style="width: 20%; background-color: transparent;"|
-===3/20/13===
+Overview
-===3/22/13===
+Winter
-===3/25/13===
+[[Team:BYU Provo/Notebook/SmallPhage/Springexp|Spring]]
-===3/27/13===
+Summer
-===3/29/13===
+Fall
-===experiment===
+| style="width: 60%; background-color: transparent;"|
+'''add overview'''
-==April==
+Description of our purpose and approaches
-===4/1/13===
-===4/4/13===
+'''Pages that needs deleting'''
-===4/5/13===
+[[Spring]] [[Winter]]
-===4/8/13===
+'''Things to figure out'''
-===4/10/13===
+How to format table?
-===4/12/13===
+How to format text besides default?
-===4/15/13===
+Domain/URL?
-==May==
+text alignment in table
-===5/1/13===
-===5/3/13===
+| style="width: 20%; background-color: transparent;"|
+'''add picture'''
-===5/6/13===
+|}
-===5/8/13===
-===5/10/13===
+==March==
-===5/13/13===
-===5/15/13===
+==April==
-===5/17/13===
-===5/20/13===
-===5/22/13===
+==May==
-===5/24/13===
+===5/1/13===
-===5/27/13===
+- Commencement of spring!!!
-===5/29/13===
+- Discussed goals and outlined plans for spring term
-===5/31/13===
+===5/2/13===
-==June==
+- Designed primers for amplifying and sequencing phage capsid protein
-===6/3/13===
-===6/5/13===
+- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
-===6/7/13===
+===5/3/13===
-===6/10/13===
+- Performed agar test, focusing primarily on ×8
-===6/12/13===
+: [[5.3 T7 phage selection method test]]
-===6/14/13===
+- Processed phage amplification
-===6/17/13===
+: [[5.3 T7 phage amplification/purification]]
-===6/19/13===
+===5/4/13===
-===6/21/13===
+- Performed dilution series using stock 5.3 (-1 through -11)
-===6/24/13===
+- Started two 5mL overnights of BL21
-===6/26/13===
+===5/5/13===
-===6/28/13===
+- Spot test using stock 5.3 and its dilution series (from 5.4)
-==July==
+: [[5.3 T7 phage amplification/purification]]
-===7/1/13===
-===7/3/13===
+- Started liquid culture for purification team (at around noon)
-===7/5/13===
+: 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
-===7/8/13===
+- Started 5.5 amplification from a plaque test
-===7/10/13===
+: [[5.5 Amplification from a plaque test]]
-===7/12/13===
+===5/6/13===
-===7/15/13===
+- Continued [[5.3 T7 phage amplification/purification]]
-===7/17/13===
+- Performed liquid culture phage concentration test
-===7/19/13===
+: [[5.6 T7+ Liquid Culture Phage Concentration Test]]
-===7/22/13===
+===5/7/13===
-===7/24/13===
+- Started two 5mL of E coli BL21 overnight
-===7/26/13===
+- Designed procedure for applying mutagen and selecting for T7
-===7/29/13===
+===5/8/13===
-===7/31/13===
+- Went over procedure for applying mutagen and PCR with Dr. Grose
-==August==
+- Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
-===8/2/13===
-===8/5/13===
+- Started two 5mL BL21 overnights
-===8/7/13===
+- Learned how to create LB plates
-===8/9/13===
+===5/9/13===
-===8/12/13===
+- Practiced with ×6 and ×8 top agar
-===8/14/13===
+: [[5.9 T7 selection method test #2]]
-===8/16/13===
+- Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
-===8/19/13===
+- Discussed plans and schedule for next week.
-===8/21/13===
+===5/20/13===
-===8/23/13===
+LP, XL
-===8/26/13===
+- We performed T7 Mutagen Concentration Test
-===8/28/13===
+: [[5.20 Mutagen Concentration Experiment]]
-===8/30/13===
+- We performed T7 Minor Capsid Protein PCR
+: [[5.20 T7 Minor Capsid Protein PCR]]
-==September==
+===5/21/13===
-===9/2/13===
-===9/4/13===
+XL
-===9/6/13===
+- started two 5mL E coli BL21 overnight at around 7:00pm
-===9/9/13===
-===9/11/13===
+===5/22/13===
-===9/13/13===
+- Continued with [[5.20 Mutagen Concentration Experiment|T7 Mutagen Concentration Test]]
-===9/16/13===
+- Continued with [[5.20 T7 Minor Capsid Protein PCR|T7 Minor Capsid Protein PCR ]] by running an agarose gel to confirm that we got the desired PCR product.
-===9/18/13===
+==June==
-===9/20/13===
-===9/23/13===
-===9/25/13===
+==July==
-===9/27/13===
-===9/30/13===
+==August==
+==September==
 {{TeamBYUProvoFooter}}