Team:Glendale CC AZ/Notebook

From 2013.igem.org

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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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-
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 +
    <title>
 +
      Notebook
 +
    </title>
 +
    <link href=
 +
    'http://fonts.googleapis.com/css?family=Open+Sans:400,300,300italic,400italic,600,600italic,700,700italic'
 +
    rel='stylesheet' type='text/css' />
 +
  </head>
 +
  <body>
 +
    <div class="content-area">
 +
      <a href="https://igem.org/Main_Page"><img style=
 +
      "border: 0px solid ; width: 50px; height: 40px;" alt="iGEM"
 +
      src=
 +
      "http://s21.postimg.org/ff5nkjy9v/IGEM_basic_Logo_stylized.png"
 +
      align="left" /></a>
 +
      <h4>
 +
        Glendale Community College Arizona<a href=
 +
        "http://www2.gccaz.edu"><img style=
 +
        "width: 200px; height: 58px;" alt="GCC" src=
 +
        "https://static.igem.org/mediawiki/2013/f/f1/Gcclogo.gif" align=
 +
        "right" /></a>
 +
      </h4>
 +
      <h3>
 +
        <big style="color: rgb(204, 0, 0);">Protocols</big>
 +
      </h3>
 +
      <hr />
 +
      <img style="border: 0px solid ; width: 549px; height: 221px;" alt="iGEM" src="http://s6.postimg.org/ac6n5yrpd/Calendar.png" align="right">
 +
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve  Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/NaCl">NaCl Growth Curve Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth">Survival Growth  Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/RestrictionDigest">Restriction Digest</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/DNAIsolation">DNA Isolation</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Bioinformatics">Bioinformatics</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation">Ligation</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Transformation">Transformation</a></p><hr>
 +
      <h3>
 +
        <big style="color: rgb(204, 0, 0);">Daily Logs</big>
 +
      </h3><!-- The Timeline -->
 +
      <ul class="timeline">
 +
        <!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 22nd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed PCR on LEA 1 and LEA 2 from D.
 +
              hopiensis. 2. Inoculated bacteria containing single
 +
              parts (LacI, RBS, TT, LEA-soybean, and RFP).
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 20th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Gel Electrophoresis on PCR products (Ran
 +
              gel too long, had to re-run). 2. Performed Alkaline
 +
              Lysis Plasmid Miniprep on sample B of single parts
 +
              (LacI promoter, RBS, LEA, TT, and the RFP control).
 +
              3. Prepared 2x 40 mL gels for mini prep products from
 +
              8/17/13 and earlier today. 4. Read absorbance values
 +
              for miniprep samples from today. 5. Performed PCR on
 +
              miniprep products from 8/17/13 as well as miniprep
 +
              products from earlier today. 6. Prepared samples for
 +
              sequencing.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 17th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Reviewed what we've learned from gel ran on
 +
              8/16/13. 2. Performed Alkaline Lysis Plasmid Miniprep
 +
              on sample A of single parts(LacI promoter, RBS, LEA,
 +
              TT, and the RFP control). 3. Read absorbance values
 +
              of miniprep products.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 16th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Gel Electrophoresis containing digest
 +
              products from 8/15/13.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 15th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed control restriction digest containing
 +
              RFP- control ONLY, but different enzymes and
 +
              different buffers for each. (To ensure enzymes and
 +
              buffers are performing as expected).
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 14th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Discussed and planned future of GCC's 2013 iGEM
 +
              team.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 13th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed PCR of LEA 1 and LEA 2 D. radiodurans
 +
              isolates. 2. Performed Gel Electrophoresis containing
 +
              8/12/13 PCR products. 3. Performed Gel
 +
              Electrophoresis containing PCR products from today
 +
              (LEA 1 &amp; 2- D. radiodurans).
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 12th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed PCR of LEA 1 and LEA 2 D. hopiensis
 +
              isolates.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 10th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team met at local public library to divide up wiki
 +
              sections and create a complete outline of project
 +
              section of the wiki.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 8th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed transformation of all ligation products
 +
              from 8/7/13 according to iGEM's transformation
 +
              protocol. 2. Found a few extra biobricks to order
 +
              from iGEM for easier assembly.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 7th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed ligation of single and double digests
 +
              according to iGEM's ligation protocol.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 6th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran out agarose gel containing PCR products from
 +
              8/1/13 (isolates+LEA). 2. Performed Gel
 +
              Electrophoresis (ran gel) containing Ecor1 digests,
 +
              as well as select single/double digested parts from
 +
              previous trials.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 5th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Debriefed team on results from all procedures
 +
              performed 8/1/13. 2. Performed Ecor1 digest using
 +
              miniprep products from 8/1/13. 3. Re-ran agarose gel
 +
              of double digested parts from 8/1/13.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">August 1st</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Restriction Digest. 2. Performed
 +
              Alkaline Lysis Plasmid Miniprep on double parts. 3.
 +
              Read absorbance values of miniprep products. 4. Ran
 +
              agarose gel containing digested parts (from earlier
 +
              today). 5. Performed PCR on single and double parts
 +
              (LacI promoter, ribosome binding site, double
 +
              terminator, RFP, LacI+ RBS, TT+ LEA) 6. Performed PCR
 +
              on D. hopiensis isolates and LEA. 7. Ran out 2x
 +
              agarose gels containing (separately) PCR products
 +
              from today.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 31st</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Restriction Digest according to values
 +
              determined on 7/29/13. 2. Inoculated bacterial
 +
              cultures from 7/25/13 plates.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 30th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran large agarose gel of PCR products from
 +
              7/25/13. 2. Performed PCR on yesterday's miniprep
 +
              products. 3. Performed PCR on LEA (D. hopiensis). 4.
 +
              Ran agarose gel of PCR products containing miniprep
 +
              products from 7/29/13.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 29th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Alkaline Lysis Plasmid Miniprep on RFP
 +
              Control, LacI Promoter, Double Terminator, and
 +
              Ribosome Binding Site. 2. Read absorbance values of
 +
              miniprep products. 3. Determined volume of miniprep
 +
              products needed to perform Restriction Digest.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 25th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Streaked plates with archived glycerol stocks. 2.
 +
              Performed PCR.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 24th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran miniprep samples on agarose gels prepared
 +
              7/22/13.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 23rd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Alkaline Lysis Plasmid Miniprep on the
 +
              rest of the transformed bacteria from 7/18/13. 2.
 +
              Read the absorbance values for all miniprep samples.
 +
              3. Read the absorbance values for genomic DNA
 +
              isolated on 7/18/13. 4. Performed Restriction Digest
 +
              on miniprep samples.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 22nd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Alkaline Lysis Plasmid Miniprep on half
 +
              of the transformed bacteria from 7/18/13. 2. Prepared
 +
              2x 50 mL agarose gels to run all miniprep samples.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 19th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. First round of wiki assignments due.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 18th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team split up to work on transformation and DNA
 +
              isolation.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 17th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran gel of digest products to ensure digest was
 +
              successful. 2. Performed ligation according to iGEM's
 +
              ligation protocol.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 16th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Restriction Digest according to iGEM's
 +
              restriction digest protocol. 2. Reviewed iGEM's
 +
              ligation protocol and created table for 7/17/13.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 15th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran gel of of 7/9/13 and 7/10/13 Miniprep
 +
              products. 2. Ran gel of PCR products from 7/10/13 3.
 +
              Presentation on Restriction Digest protocol and
 +
              created table for 7/16/13 digest.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 10th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Counted colonies on plates from yesterday's
 +
              Survival Growth Assay. 2. Performed PCR 3. Performed
 +
              Miniprep.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 9th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed NaCl Growth Curve Assay on E.coli
 +
              containing PprA and RecA. 2. Ran gels of PCR
 +
              products. 3. Performed Miniprep. 4. Performed
 +
              Survival Growth Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 8th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Set up bacterial growth culture of transformation
 +
              products from 7/8/13. 2. Performed Polymerase Chain
 +
              Reaction.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 3rd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed transformation on LacI promoter
 +
              (BBa_R0010), Ribosome Binding Site (BBa_B0034),
 +
              Double Terminator (BBa_B0015), and RFP Control
 +
              provided by iGEM.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 2nd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team split up and performed NaCl Growth Curve
 +
              Assay on transformed E.coli containing PprI gene or
 +
              RecA gene (depending on group). 2. Performed Survival
 +
              Growth Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">July 1st</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Presentation on adding information and navigating
 +
              our wiki page. 2. First round of wiki sections
 +
              assigned.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 27th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed NaCl Growth Curve Assay, working with
 +
              higher concentration of IPTG. 2. Performed Survival
 +
              Growth Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 26th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Ran yesterday's miniprep products out on a gel. 2.
 +
              Performed NaCl Growth Curve Assay. 3. Developed
 +
              protocol and flowchart for Survival Growth Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 25th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Miniprep.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 20th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Miniprep and Gel Electrophoresis.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 19th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Presentation on Gel Electrophoresis protocol.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 18th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Added prefix and suffix sequences to primers,
 +
              reviewed entire sequence once more prior to ordering.
 +
              2. Presentation on Alkaline Lysis Plasmid Miniprep
 +
              protocol, drew up flowchart.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 17th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. De-briefed members unable to attend ASU luncheon.
 +
              2. Fern and Cristina debuted first clips of our team
 +
              stop motion video!
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 14th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team went to ASU for meet-and-greet luncheon with
 +
              ASU's 2013 iGEM team.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 13th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Hydrogen Peroxide Growth Curve Assay,
 +
              still searching working with different concentrations
 +
              of hydrogen peroxide. 2. Presentation on iGEM's
 +
              transformation protocol.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 12th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team double checked primer designs for restriction
 +
              sites Xba1, Pst1, Ecor1, and Spe1.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 11th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Hydrogen Peroxide Growth Curve Assay
 +
              with different concentrations of hydrogen peroxide.
 +
              2. Team searched for homolog genes of interest in D.
 +
              hopiensis.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 10th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Team presented primer designs.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 6th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed NaCl Growth Curve Assay to determine
 +
              appropriate concentration of NaCl. 2. Planned next
 +
              stages of our project.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 5th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Performed Hydrogen Peroxide Growth Curve Assay to
 +
              determine appropriate concentration of hydrogen
 +
              peroxide.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">June 4th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Reviewed protocol for DNA isolation. 2. Biotech
 +
              student Beau came in to present his project on
 +
              Deinococcus radiodurans.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">May 29th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Biobrick and gene presentations continued. 2. Team
 +
              covered requirements for designing primers,
 +
              determined primers for all genes of interest.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">May 28th</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Biobrick and gene presentations continued. 2.
 +
              Reviewed protocol for Polymerase Chain Reaction.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">May 22nd</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Members continued biobrick and gene presentations.
 +
              2. Team split into 5 groups and performed our first
 +
              Growth Curve Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item A -->
 +
        <li>
 +
          <div class="direction-r">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">May 21st</span> <span class=
 +
              "time-wrapper"><span class="time">2013</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              1. Members began their biobrick presentations. 2. We
 +
              covered online resources available to the team such
 +
              as Open Wetware, iGEM Help pages, and Rstudio. 3.
 +
              Team developed protocol for our Growth Curve Assay.
 +
            </div>
 +
          </div>
 +
        </li><!-- Item B -->
 +
        <li>
 +
          <div class="direction-l">
 +
            <div class="flag-wrapper">
 +
              <span class="flag">1st Meeting</span> <span class=
 +
              "time-wrapper"><span class="time">May
 +
              20th</span></span>
 +
            </div>
 +
            <div class="desc">
 +
              <p list="">
 +
                1. Developed goals for the summer 2. Reviewed lab
 +
                techniques 3. Reviewed iGEM website and registry 4.
 +
                Assigned biobricks of interest and genes of
 +
                interest to present
 +
              </p>
 +
            </div>
 +
          </div>
 +
        </li>
 +
      </ul>
 +
    </div><!--/.content-area-->
 +
  </body>
 +
</html>

Latest revision as of 02:33, 28 September 2013

Notebook

iGEM

Glendale Community College ArizonaGCC

Protocols

iGEM Growth Curve Assay

NaCl Growth Curve Assay

Survival Growth Assay

Alkaline Lysis Plasmid Miniprep

Restriction Digest

DNA Isolation

Bioinformatics

Ligation

Transformation

Daily Logs

  • August 22nd 2013
    1. Performed PCR on LEA 1 and LEA 2 from D. hopiensis. 2. Inoculated bacteria containing single parts (LacI, RBS, TT, LEA-soybean, and RFP).
  • August 20th 2013
    1. Performed Gel Electrophoresis on PCR products (Ran gel too long, had to re-run). 2. Performed Alkaline Lysis Plasmid Miniprep on sample B of single parts (LacI promoter, RBS, LEA, TT, and the RFP control). 3. Prepared 2x 40 mL gels for mini prep products from 8/17/13 and earlier today. 4. Read absorbance values for miniprep samples from today. 5. Performed PCR on miniprep products from 8/17/13 as well as miniprep products from earlier today. 6. Prepared samples for sequencing.
  • August 17th 2013
    1. Reviewed what we've learned from gel ran on 8/16/13. 2. Performed Alkaline Lysis Plasmid Miniprep on sample A of single parts(LacI promoter, RBS, LEA, TT, and the RFP control). 3. Read absorbance values of miniprep products.
  • August 16th 2013
    1. Performed Gel Electrophoresis containing digest products from 8/15/13.
  • August 15th 2013
    1. Performed control restriction digest containing RFP- control ONLY, but different enzymes and different buffers for each. (To ensure enzymes and buffers are performing as expected).
  • August 14th 2013
    1. Discussed and planned future of GCC's 2013 iGEM team.
  • August 13th 2013
    1. Performed PCR of LEA 1 and LEA 2 D. radiodurans isolates. 2. Performed Gel Electrophoresis containing 8/12/13 PCR products. 3. Performed Gel Electrophoresis containing PCR products from today (LEA 1 & 2- D. radiodurans).
  • August 12th 2013
    1. Performed PCR of LEA 1 and LEA 2 D. hopiensis isolates.
  • August 10th 2013
    1. Team met at local public library to divide up wiki sections and create a complete outline of project section of the wiki.
  • August 8th 2013
    1. Performed transformation of all ligation products from 8/7/13 according to iGEM's transformation protocol. 2. Found a few extra biobricks to order from iGEM for easier assembly.
  • August 7th 2013
    1. Performed ligation of single and double digests according to iGEM's ligation protocol.
  • August 6th 2013
    1. Ran out agarose gel containing PCR products from 8/1/13 (isolates+LEA). 2. Performed Gel Electrophoresis (ran gel) containing Ecor1 digests, as well as select single/double digested parts from previous trials.
  • August 5th 2013
    1. Debriefed team on results from all procedures performed 8/1/13. 2. Performed Ecor1 digest using miniprep products from 8/1/13. 3. Re-ran agarose gel of double digested parts from 8/1/13.
  • August 1st 2013
    1. Performed Restriction Digest. 2. Performed Alkaline Lysis Plasmid Miniprep on double parts. 3. Read absorbance values of miniprep products. 4. Ran agarose gel containing digested parts (from earlier today). 5. Performed PCR on single and double parts (LacI promoter, ribosome binding site, double terminator, RFP, LacI+ RBS, TT+ LEA) 6. Performed PCR on D. hopiensis isolates and LEA. 7. Ran out 2x agarose gels containing (separately) PCR products from today.
  • July 31st 2013
    1. Performed Restriction Digest according to values determined on 7/29/13. 2. Inoculated bacterial cultures from 7/25/13 plates.
  • July 30th 2013
    1. Ran large agarose gel of PCR products from 7/25/13. 2. Performed PCR on yesterday's miniprep products. 3. Performed PCR on LEA (D. hopiensis). 4. Ran agarose gel of PCR products containing miniprep products from 7/29/13.
  • July 29th 2013
    1. Performed Alkaline Lysis Plasmid Miniprep on RFP Control, LacI Promoter, Double Terminator, and Ribosome Binding Site. 2. Read absorbance values of miniprep products. 3. Determined volume of miniprep products needed to perform Restriction Digest.
  • July 25th 2013
    1. Streaked plates with archived glycerol stocks. 2. Performed PCR.
  • July 24th 2013
    1. Ran miniprep samples on agarose gels prepared 7/22/13.
  • July 23rd 2013
    1. Performed Alkaline Lysis Plasmid Miniprep on the rest of the transformed bacteria from 7/18/13. 2. Read the absorbance values for all miniprep samples. 3. Read the absorbance values for genomic DNA isolated on 7/18/13. 4. Performed Restriction Digest on miniprep samples.
  • July 22nd 2013
    1. Performed Alkaline Lysis Plasmid Miniprep on half of the transformed bacteria from 7/18/13. 2. Prepared 2x 50 mL agarose gels to run all miniprep samples.
  • July 19th 2013
    1. First round of wiki assignments due.
  • July 18th 2013
    1. Team split up to work on transformation and DNA isolation.
  • July 17th 2013
    1. Ran gel of digest products to ensure digest was successful. 2. Performed ligation according to iGEM's ligation protocol.
  • July 16th 2013
    1. Performed Restriction Digest according to iGEM's restriction digest protocol. 2. Reviewed iGEM's ligation protocol and created table for 7/17/13.
  • July 15th 2013
    1. Ran gel of of 7/9/13 and 7/10/13 Miniprep products. 2. Ran gel of PCR products from 7/10/13 3. Presentation on Restriction Digest protocol and created table for 7/16/13 digest.
  • July 10th 2013
    1. Counted colonies on plates from yesterday's Survival Growth Assay. 2. Performed PCR 3. Performed Miniprep.
  • July 9th 2013
    1. Performed NaCl Growth Curve Assay on E.coli containing PprA and RecA. 2. Ran gels of PCR products. 3. Performed Miniprep. 4. Performed Survival Growth Assay.
  • July 8th 2013
    1. Set up bacterial growth culture of transformation products from 7/8/13. 2. Performed Polymerase Chain Reaction.
  • July 3rd 2013
    1. Performed transformation on LacI promoter (BBa_R0010), Ribosome Binding Site (BBa_B0034), Double Terminator (BBa_B0015), and RFP Control provided by iGEM.
  • July 2nd 2013
    1. Team split up and performed NaCl Growth Curve Assay on transformed E.coli containing PprI gene or RecA gene (depending on group). 2. Performed Survival Growth Assay.
  • July 1st 2013
    1. Presentation on adding information and navigating our wiki page. 2. First round of wiki sections assigned.
  • June 27th 2013
    1. Performed NaCl Growth Curve Assay, working with higher concentration of IPTG. 2. Performed Survival Growth Assay.
  • June 26th 2013
    1. Ran yesterday's miniprep products out on a gel. 2. Performed NaCl Growth Curve Assay. 3. Developed protocol and flowchart for Survival Growth Assay.
  • June 25th 2013
    1. Performed Miniprep.
  • June 20th 2013
    1. Performed Miniprep and Gel Electrophoresis.
  • June 19th 2013
    1. Presentation on Gel Electrophoresis protocol.
  • June 18th 2013
    1. Added prefix and suffix sequences to primers, reviewed entire sequence once more prior to ordering. 2. Presentation on Alkaline Lysis Plasmid Miniprep protocol, drew up flowchart.
  • June 17th 2013
    1. De-briefed members unable to attend ASU luncheon. 2. Fern and Cristina debuted first clips of our team stop motion video!
  • June 14th 2013
    1. Team went to ASU for meet-and-greet luncheon with ASU's 2013 iGEM team.
  • June 13th 2013
    1. Performed Hydrogen Peroxide Growth Curve Assay, still searching working with different concentrations of hydrogen peroxide. 2. Presentation on iGEM's transformation protocol.
  • June 12th 2013
    1. Team double checked primer designs for restriction sites Xba1, Pst1, Ecor1, and Spe1.
  • June 11th 2013
    1. Performed Hydrogen Peroxide Growth Curve Assay with different concentrations of hydrogen peroxide. 2. Team searched for homolog genes of interest in D. hopiensis.
  • June 10th 2013
    1. Team presented primer designs.
  • June 6th 2013
    1. Performed NaCl Growth Curve Assay to determine appropriate concentration of NaCl. 2. Planned next stages of our project.
  • June 5th 2013
    1. Performed Hydrogen Peroxide Growth Curve Assay to determine appropriate concentration of hydrogen peroxide.
  • June 4th 2013
    1. Reviewed protocol for DNA isolation. 2. Biotech student Beau came in to present his project on Deinococcus radiodurans.
  • May 29th 2013
    1. Biobrick and gene presentations continued. 2. Team covered requirements for designing primers, determined primers for all genes of interest.
  • May 28th 2013
    1. Biobrick and gene presentations continued. 2. Reviewed protocol for Polymerase Chain Reaction.
  • May 22nd 2013
    1. Members continued biobrick and gene presentations. 2. Team split into 5 groups and performed our first Growth Curve Assay.
  • May 21st 2013
    1. Members began their biobrick presentations. 2. We covered online resources available to the team such as Open Wetware, iGEM Help pages, and Rstudio. 3. Team developed protocol for our Growth Curve Assay.
  • 1st Meeting May 20th

    1. Developed goals for the summer 2. Reviewed lab techniques 3. Reviewed iGEM website and registry 4. Assigned biobricks of interest and genes of interest to present

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Notebook"