Team:INSA Toulouse

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Abstract, let's count with ''E. Calculus''</div>
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Transposition of Boolean operators into genetic devices is one of the various goals of Synthetic Biology. The common problem with genetic Boolean operators is their inherent inability to make a clear difference between the 0 and 1 state. A new way to build one-way logical gates using Serine recombinases will be implemented, taking advantage of specific recombination sites to avoid the reversibility of recombination. Furthermore, we will also implement a strong genetic switch to avoid transcriptional background and this will lead to a perfectly controlled transcription of the recombinases. These systems should approach as much as possible a two digit device and help setting strong and robust genetic logical gates. To demonstrate the validity of these two state logic gates, we will create a n-bits full-adder using recombinase-based AND and XOR logical gates. The E. calculus strain should be able to execute full n-bits counting, taking in account the carry at each stage.
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<br>Irreversibility of recombination allows us to get great signal amplification (devices should be transmitted to next generation), with only two possible states. The general idea of those kinds of genetic logical gates is the use of genetics elements placed in different ways (forward or reverse) between specific recombinational sites. Expression or not of recombinase (input signals) should induce change (or not) state of the gate, which allow us to get an answer (output signal). We also try to link different gates between them, aiming finally to construct a full adder. We know that recombinases are actives at even in low concentration in cell (3 or 4 recombinases are enough to operate recombination). We have to control strenghtly recombinases expression. We choose traductional control with use of fresh-designed riboswitches, avoiding this way probables leaks of a transcriptional control.
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      <h1 class="h1project">Project</h1>
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      <p>One major goal of Synthetic Biology is the transposition of electronic devices such as logic gates into genetic modules capables of complex, autonomous decisions. Very recent publications described highly original logic gates utilizing serine recombinases, avoiding reversibility of the genetic switch and thus creating strong and robust genetic logical gates (AND, XOR, OR). <br> The <i>E. calculus</i> project exemplifies these genetic modules by creating a binary full-adder using AND and XOR gates. Our envisionned design could theoritically perform n-bits counting with a carry. Our project also aimed at validating original properties of these new genetic switches: irreversibility and accurate genetic transmission of the logic gate switched state to the cell'offspring.</p>
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              <div class="textnew"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/extras/achievements" style="color:grey;"><p>Check out our ACHIEVEMENTS page!! Watch out for this!!</p></a></div>
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              <div class="textnew"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results" style="color:grey;"><p>AND1 and XOR1 gates switched!!!!</p></a></div>
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              <div class="textnew"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/extras/fun_facts" style="color:grey;"><p>Check out our Fun Facts page!!</p></a></div>
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              <div class="textnew"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/team/students" style="color:grey;"><p>If you want to know a little bit more about the team, look at this! You may discover magic pictures if you put your mouse over the pictures...</p></a></div>
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Latest revision as of 01:53, 5 October 2013

logo

Project

One major goal of Synthetic Biology is the transposition of electronic devices such as logic gates into genetic modules capables of complex, autonomous decisions. Very recent publications described highly original logic gates utilizing serine recombinases, avoiding reversibility of the genetic switch and thus creating strong and robust genetic logical gates (AND, XOR, OR).
The E. calculus project exemplifies these genetic modules by creating a binary full-adder using AND and XOR gates. Our envisionned design could theoritically perform n-bits counting with a carry. Our project also aimed at validating original properties of these new genetic switches: irreversibility and accurate genetic transmission of the logic gate switched state to the cell'offspring.

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Gallery Project

News

Check out our ACHIEVEMENTS page!! Watch out for this!!

AND1 and XOR1 gates switched!!!!

Check out our Fun Facts page!!

If you want to know a little bit more about the team, look at this! You may discover magic pictures if you put your mouse over the pictures...


Retrieved from "http://2013.igem.org/Team:INSA_Toulouse"