Exeter/29 July 2013

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== Liquid cultures ==
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{{:Team:Exeter/Template/Header}}
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We made liquid cultures of:
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Cph8 + RBS - K592018
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{{:Team:Exeter/Template/Footer}}
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B0015 - Terminator
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K592022 - Cyan (w/RBS and promoter)
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K864404 - Promoter, RBS and cyan
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K592011 - Cyan pigment
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S05058
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== Digest ==
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We followed advice from a PhD student and did:
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* Positive controls of:
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- RFP cut with E and S
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- RFP cut with X and P
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* Negative control of:
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- Just water
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The buffer and BSA were vortexed before use to make sure they were completely defrosted.
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We got fresh purite water each time we needed it.
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We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.
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Latest revision as of 07:18, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

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Exeter iGEM 2013 · Paint by Coli
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