Team:UCSF/kendall

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display: block;
 +
font-family: Myriad Pro, Gill Sans MT, Trebuchet MS, Arial, Sans-Serif;
 +
color: white;
 +
}
 +
#nav li a{
 +
line-height: 1.4em; /*centers the text vertically*/
 +
font-size: 2em;
 +
}
 +
#nav ul li > a{
 +
width: 200px;
}
}
-
+
/*color change after rollover*/
-
// Check which current image we need to show
+
#nav li a:hover, #nav li li a.drop:hover::after{
-
if(direction == "next") {
+
display: block;
-
currentImg++;
+
text-decoration: none;
-
if(currentImg == photos.length + 1) {
+
color: #43bbff;
-
currentImg = 1;
+
}
-
}
+
#nav li ul li ul{
-
} else {
+
margin-top: -32px;
-
currentImg--;
+
position: absolute;
-
if(currentImg == 0) {
+
}
-
currentImg = photos.length;
+
/*submenu and sub-submenu*/
-
}
+
#nav li ul li ul li a, #nav li ul li a{
 +
font-size: 1.8em;
 +
}
 +
#nav li li a.drop:after{
 +
content: '>';
 +
padding-left: 20px;
 +
color: #BBB;
 +
display: inline;
 +
float: right;
}
}
-
 
-
// Check which container we need to use
 
-
var currentContainer = activeContainer;
 
-
if(activeContainer == 1) {
 
-
activeContainer = 2;
 
-
} else {
 
-
activeContainer = 1;
 
-
}
 
-
 
-
showImage(photos[currentImg - 1], currentContainer, activeContainer);
 
-
 
-
};
 
-
 
-
var currentZindex = -1;
 
-
var showImage = function(photoObject, currentContainer, activeContainer) {
 
-
animating = true;
 
-
 
-
// Make sure the new container is always on the background
 
-
currentZindex--;
 
-
 
-
// Set the background image of the new active container
 
-
$("#headerimg" + activeContainer).css({
 
-
"background-image" : "url(images/" + photoObject.image + ")",
 
-
"display" : "block",
 
-
"z-index" : currentZindex
 
-
});
 
-
 
-
// Hide the header text
 
-
$("#headertxt").css({"display" : "none"});
 
-
 
-
// Set the new header text
 
-
$("#firstline").html(photoObject.firstline);
 
-
$("#secondline")
 
-
.attr("href", photoObject.url)
 
-
.html(photoObject.secondline);
 
-
$("#pictureduri")
 
-
.attr("href", photoObject.url)
 
-
.html(photoObject.title);
 
-
 
-
 
-
// Fade out the current container
 
-
// and display the header text when animation is complete
 
-
$("#headerimg" + currentContainer).fadeOut(function() {
 
-
setTimeout(function() {
 
-
$("#headertxt").css({"display" : "block"});
 
-
animating = false;
 
-
}, 500);
 
-
});
 
-
};
 
-
 
-
var stopAnimation = function() {
 
-
// Change the background image to "play"
 
-
$("#control").css({ "background-image" : "url(images/btn_play.png)" });
 
-
 
-
// Clear the interval
 
-
clearInterval(interval);
 
-
};
 
-
 
-
// We should statically set the first image
 
-
navigate("next");
 
-
 
-
// Start playing the animation
 
-
interval = setInterval(function() {
 
-
navigate("next");
 
-
}, slideshowSpeed);
 
-
 
-
});
 
 +
/***End nav styling***/
-
</script>
+
/***Headerimage***/
 +
#headerimage{
 +
width: 968px;
 +
position: relative;
 +
margin-left: 0px;
 +
margin-bottom: 10px;
 +
top: 0px;
 +
}
 +
#css-full{
 +
display: none;
 +
}
 +
#css-mobi{
 +
display: block;
 +
top: 0px;
 +
}
 +
/***Logo styling***/
 +
#logo{
 +
position: absolute;
 +
top: 10px;
 +
right: 20px;
 +
float: right;
 +
}
 +
#logo img{
 +
width: 260px;
 +
}
 +
 +
/***Body styling***/
 +
h1{
 +
font-size: 2.3em;
 +
line-height: 1em;
 +
}
 +
h2{
 +
font-size: 1.8em;
 +
line-height: 1.2em;
 +
}
 +
h3{
 +
font-size: 1.6em;
 +
margin: 0px 15px;
 +
font-weight: bold;
 +
}
 +
h4{
 +
font-size: 1.4em;
 +
color: #333333;
 +
margin: 0px 20px;
 +
font-weight: bold;
 +
}
 +
#bodycontainer p{
 +
font-size: 1.2em;
 +
}
 +
#pagetitle{
 +
border-bottom: 2px solid black;
 +
padding-bottom: 10px;
 +
padding-left: 10px;
 +
}
 +
#bodycontainer h2{
 +
margin-left: 10px;
 +
margin-right: 10px;
 +
}
 +
#bodycontainer p{
 +
margin-left: 20px;
 +
margin-right: 10px;
 +
}
 +
#bodycontainer{
 +
margin-left: 220px;
 +
}
 +
#bodycontainer ul{
 +
margin-left: 5.0em;
 +
}
 +
#bodycontainer li{
 +
font-family: Georgia, Serif;
 +
}
 +
#sidebar{
 +
position: absolute;
 +
width: 210px;
 +
z-index: 0;
 +
}
 +
/*
 +
#sidebarimg{
 +
width: 214px;
 +
height: 144px;
 +
background: #43bbff;
 +
margin: 5px 0px;
 +
border: 3px solid #333333;
 +
}
 +
*/
 +
#sidebar #list{
 +
background: #333333;
 +
}
 +
#sidebar h2{
 +
color: white;
 +
padding: 20px 15px 0px 15px;
 +
font-size: 2.0em;
 +
}
 +
#sidebar ul{
 +
list-style: none;
 +
margin: 0px 15px;
 +
}
 +
#sidebar #list > ul{
 +
padding-bottom: 20px;
 +
}
 +
#sidebar a{
 +
color: white;
 +
font-family: Georgia, Serif;
 +
font-size: 1.4em;
 +
display: block;
 +
line-height: 1.4em;
 +
}
 +
#sidebar a:hover{
 +
text-decoration: none;
 +
color: #43BBFF;
 +
}
-
 
+
/* thumbnails */
-
 
+
div.thumb {
-
<div id="navigation"></div>
+
    margin-bottom: .5em;
-
 
+
    border-style: solid;
 +
    border-color: transparent;
 +
    width: auto;
 +
}
 +
div.thumb div {
 +
    border: 0px;
 +
    padding: 3px !important;
 +
    font-size: 94%;
 +
    text-align: center;
 +
    overflow: hidden;
 +
background: transparent;
 +
}
 +
div.thumb div a img {
 +
    border: none;
 +
}
 +
div.thumb div div.thumbcaption {
 +
    border: none;
 +
    text-align: left;
 +
    line-height: 1.4em;
 +
    padding: .3em 0 .1em 0;
 +
}
 +
div.magnify {
 +
    float: right;
 +
    border: none !important;
 +
    background: none !important;
 +
}
 +
div.magnify a, div.magnify img {
 +
    display: block;
 +
    border: none !important;
 +
    background: none !important;
 +
}
 +
div.tright {
 +
    clear: right;
 +
    float: right;
 +
    border-width: .5em 0 .8em 1.4em;
 +
}
 +
div.tleft {
 +
    float: left;
 +
    margin-right: .5em;
 +
    border-width: .5em 1.4em .8em 0;
 +
}
 +
/*colouring*/
 +
/*oranges: current page and all sidebar rollovers*/
 +
#home header #homelink, #home #sidebar #list a:hover, #home #nav li a:hover, #home #nav li a.drop:hover::after,
 +
#team header #teamlink, #team #sidebar #list a:hover, #team #nav li a:hover, #team #nav li a.drop:hover::after,
 +
#proj_hp header #projectlink, #proj_hp #sidebar #list a:hover, #proj_hp #nav li a:hover, #proj_hp #nav li a.drop:hover::after,
 +
#parts header #partslink, #parts #sidebar #list a:hover, #parts #nav li a:hover, #parts #nav li a.drop:hover::after,
 +
#notebook header #notebooklink, #notebook #sidebar #list a:hover, #notebook #nav li a:hover, #notebook #nav li a.drop:hover::after,
 +
#outreach header #outreachlink, #outreach #sidebar #list a:hover, #outreach #nav li a:hover, #outreach #nav li a.drop:hover::after,
 +
#sponsors header #sponsorslink, #sponsors #sidebar #list a:hover, #sponsors #nav li a:hover, #sponsors #nav li a.drop:hover::after{
 +
color: #F6A82D;
 +
}
 +
/*oranges: sidebar border*/
 +
#home #sidebar #list,
 +
#team #sidebar #list,
 +
#proj_hp #sidebar #list,
 +
#parts #sidebar #list,
 +
#notebook #sidebar #list,
 +
#outreach #sidebar #list,
 +
#sponsors #sidebar #list{
 +
border-left: 10px solid #F6A82D;
 +
}
 +
/*oranges: links*/
 +
#team #bodycontainer a,
 +
#proj_hp #bodycontainer a,
 +
#parts #bodycontainer a,
 +
#notebook #bodycontainer a,
 +
#outreach #bodycontainer a,
 +
#sponsors #bodycontainer a{
 +
color: #FF7A00;
 +
}
 +
#team #bodycontainer a:visited,
 +
#proj_hp #bodycontainer a:visited,
 +
#parts #bodycontainer a:visited,
 +
#notebook #bodycontainer a:visited,
 +
#outreach #bodycontainer a:visited,
 +
#sponsors #bodycontainer a:visited{
 +
color: #C40;
 +
}
 +
/*blues: current page, all rollover links in nav, all sidebar hovers and dropdown menus*/
 +
#proj_o header #projectlink, #proj_o #sidebar #list a:hover, #proj_o #nav li a:hover, #proj_o #nav li a.drop:hover::after,
 +
#note_o header #notebooklink, #note_o #sidebar #list a:hover, #note_o #nav li a:hover, #note_o #nav li a.drop:hover::after{
 +
color: #43BBFF;
 +
}
 +
/*blues: sidebar border*/
 +
#proj_o #sidebar #list,
 +
#note_o #sidebar #list{
 +
border-left: 10px solid #43BBFF;
 +
}
 +
/*blues: links*/
 +
#proj_o #bodycontainer a,
 +
#note_o #bodycontainer a{
 +
color: #1088CC;
 +
}
 +
#proj_o #bodycontainer a:visited,
 +
#note_o #bodycontainer a:visited{
 +
color: #05B;
 +
}
 +
/*greens: current page, all rollover links in nav, all sidebar hovers, and dropdown menus*/
 +
#proj_f header #projectlink, #proj_f #sidebar #list a:hover, #proj_f #nav li a:hover, #proj_f #nav li a.drop:hover::after,
 +
#note_f header #notebooklink, #note_f #sidebar #list a:hover, #note_f #nav li a:hover, #note_f #nav li a.drop:hover::after{
 +
color: #58CD45;
 +
}
 +
/*greens: sidebar border*/
 +
#proj_f #sidebar #list,
 +
#note_f #sidebar #list{
 +
border-left: 10px solid #58CD45;
 +
}
 +
/*greens: links*/
 +
#proj_f #bodycontainer a,
 +
#proj_f #bodycontainer a{
 +
color: #159900;
 +
}
 +
/*greens: links*/
 +
#proj_f #bodycontainer a:visited,
 +
#proj_f #bodycontainer a:visited{
 +
color: #06660B;
 +
}
 +
/*hub page CSS*/
 +
a.hublink{
 +
text-decoration: none;
 +
margin-left: 15px;
 +
margin-right: 15px;
 +
}
 +
div.hubbox{
 +
margin-left: 15px;
 +
}
 +
div.hubbox img{
 +
float: left;
 +
padding: 15px;
 +
}
 +
div.hubbox h2{
 +
color: white;
 +
padding: 15px 15px 0px 15px;
 +
font-size: 2.0em;
 +
margin-left: 120px !important;
 +
}
 +
div.hubbox p{
 +
color: white;
 +
padding: 0px 15px 15px 15px;
 +
margin-left: 120px !important;
 +
}
 +
div.hubbox b{
 +
font-size: 1.1em;
 +
}
 +
</style>
 +
<script type="text/javascript">
 +
    jQuery(document).ready(function ($) {
 +
        //eliminate jsnotice
 +
        $('#jsnotice').hide();
 +
        //prepend menu icon
 +
        $('#nav-wrap').prepend('<div id="menu-icon">Menu</div>');
 +
        //toggle nav
 +
        $("#menu-icon").click(function () {
 +
            $("#nav").slideToggle('fast');
 +
            $(this).toggleClass("active");
 +
        });
 +
        //hide url bar
 +
        window.scrollTo(0, 1);
 +
    });
 +
</script>
 +
<!--switching function: thanks to http://www.digital-web.com/articles/strategies_for_css_switching/-->
-
<link rel="stylesheet" href="https://2012.igem.org/Team:Johns_Hopkins-Software/Templates/cssindex?action=raw&amp;ctype=text/css" type="text/css"/>
+
<script type="text/javascript">
 +
    var _gaq = _gaq || [];
 +
    _gaq.push(['_setAccount', 'UA-32774032-1']);
 +
    _gaq.push(['_trackPageview']);
 +
    (function () {
 +
        var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
 +
        ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
 +
        var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
 +
    })();
 +
</script>
 +
</head>
 +
<body>
-
 
+
<header>
-
 
+
<!--
-
 
+
<a id="css-full" href="#default">Full View</a>
-
 
+
<a id="css-mobi" href="#mobile">Mobile View</a>
-
 
+
-->
-
 
+
<div id="headerimage"><img src="https://static.igem.org/mediawiki/2013/b/b8/Leaf1.png"></img></div>
-
 
+
    <a id="logo" href="https://2013.igem.org/Team:UCSF"><img src="https://static.igem.org/mediawiki/2013/b/be/Ucsf_sig_rgb_1.png"></img></a>
-
 
+
<div id="nav-wrap">
-
 
+
<ul id="nav">
-
 
+
<li><a href="https://2013.igem.org/Team:UCSF" id="homelink">Home</a></li>
-
</div>
+
<li><a class="drop" id="team">Team</a>
-
</div>
+
-
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+
-
 
+
-
 
+
-
<link rel="stylesheet" href="https://2012.igem.org/Team:Johns_Hopkins-Software/Templates/cssindex?action=raw&amp;ctype=text/css" type="text/css"/>
+
-
<div id="header_body">
+
-
<div id="header_new">
+
-
<div id="header_title">
+
-
<a href="https://2013.igem.org/Team:UCSF"><img src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/group%20photo%20on%20steps.jpg" width="65%" alt="Autogene" style="border:none; -moz-box-shadow: 0px 0px 0px #fff;
+
-
-webkit-box-shadow: 0px 0px 0px #Fff;
+
-
box-shadow: 0px 0px 0px #fff;
+
-
/* For IE 8 */
+
-
-ms-filter: "progid:DXImageTransform.Microsoft.Shadow(Strength=0, Direction=135, Color='#ffffff')";
+
-
/* For IE 5.5 - 7 */
+
-
filter: progid:DXImageTransform.Microsoft.Shadow(Strength=0, Direction=135, Color='#ffffff');"></a><a href="https://2013.igem.org/Main_Page"><img src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/extra/photo%20page/IMG_4513.JPG" width="25.9%" style="border:none; -moz-box-shadow: 0px 0px 0px #fff;
+
-
-webkit-box-shadow: 0px 0px 0px #Fff;
+
-
box-shadow: 0px 0px 0px #fff;
+
-
/* For IE 8 */
+
-
-ms-filter: "progid:DXImageTransform.Microsoft.Shadow(Strength=0, Direction=135, Color='#ffffff')";
+
-
/* For IE 5.5 - 7 */
+
-
filter: progid:DXImageTransform.Microsoft.Shadow(Strength=0, Direction=135, Color='#ffffff');"></a>
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id = "navigation_wrapper">
+
-
<div id="navigation_low">
+
-
<div id="mydroplinemenu" class="droplinebar">
+
-
<ul>
+
-
<li><a href="#">Team</a>
+
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:UCSF/Team">Members</a></li>
+
                        <li><a href="https://2012.igem.org/Team:UCSF/Background">Team Background</a></li>
-
+
<li><a href="https://2013.igem.org/Team:UCSF/Team">Members</a></li>
-
+
                        <li><a herf="https://2013.igem.org/Team:UCSF/TeamAttribution">Attribution</a><li>
 +
<li><a href="https://2012.igem.org/Team:UCSF/Advisors">Advisors</a></li>
 +
                        <li><a href="https://2012.igem.org/Team:UCSF/Mentors&amp;Instructors">Mentors</a></li>
 +
                        <li><a href="https://igem.org/Team.cgi?year=2013">Profile</a></li>
 +
                        <li><a href="https://2013.igem.org/Team:UCSF/ContactUs">Contact Us</a></li>
</ul>
</ul>
-
</li>
+
</li>
-
 
+
<li><a class="drop" href="https://2013.igem.org/Team:UCSF/Project" id="projectlink">Project</a>
-
<li><a href="#">Software</a>
+
<ul>
<ul>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/theSoftware_overview">Overview</a></li>
+
<li>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/AutoPlasmid">AutoPlasmid</a></li>
+
<a class="drop" href="https://2012.igem.org/Team:Calgary/Project">Overview</a>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/AutoDesign">AutoDesign</a></li>
+
<ul>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/Cloud">Cloud</a></li>
+
                                <li><a href="https://2013.igem.org/Team:UCSF/Project/Accomplish">Accomplishments</a></li>
-
                                        <li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/Compatibility">Compatibility</a></li>
+
                                <li><a href="https://2013.igem.org/Team:UCSF/Project/DataPage">Data Page</a></li>
-
                                        <li><a href="http://www.ugrad.cs.jhu.edu/~eisinger/iGEM/Screencast.mov">Screencast</a></li>
+
                                <li><a href="https://2012.igem.org/Team:Calgary/Parts">Parts</a></li>
-
                                        <li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/DownloadSoftware">Download</a></li>
+
                                <li><a href="https://2013.igem.org/Team:UCSF/Safety">Safety</a></li>
 +
                                <!--<li><a href="https://2012.igem.org/Team:Calgary/Project/Post-Regionals">Post-Regionals</a></li>-->
 +
</ul>
 +
</li>
 +
<li>
 +
<a class="drop" href="https://2013.igem.org/Team:UCSF/Project/Conjugation">Conjugation</a>
 +
                            <!--
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Detecting">Toxin Sensing</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting">Electroreporting</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Modelling">Modelling</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Prototype">Device Prototype</a></li>
 +
</ul>
 +
                            -->
 +
</li>
 +
<li>
 +
<a class="drop" href="https://2013.igem.org/Team:UCSF/Project/Circuit">Synthetic Circuit</a>
 +
                            <!--
 +
<ul>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Decarboxylation">Decarboxylation</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/CatecholDegradation">Decatecholization</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis">Flux Analysis</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Bioreactor">Bioreactor</a></li>
 +
<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Upgrading">Upgrading</a></li>
 +
<li><a style="margin:0 -1em 0 1em;"href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization">Desulfurization</a></li>
 +
<li><a style="margin:0 -1em 0 1em;"href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Denitrogenation">Denitrogenation</a></li>
 +
</ul>
 +
                            -->
 +
</li>
 +
<li><a href="https://2013.igem.org/Team:UCSF/Project/References">References</a></li>
</ul>
</ul>
-
</li>
+
</li>
-
 
+
                <li><a class="drop" href="https://2013.igem.org/Team:UCSF/Modeling" id="Modeling">Modeling</a></li>
-
<li><a href="#">Requirements</a>
+
                <li><a class="drop" href="https://2013.igem.org/Team:UCSF/HumanPractice" id="HumanPractive">Human Practice</a></li>
 +
<li><a class="drop" href="https://2013.igem.org/Team:UCSF/Notebook" id="notebooklink">Notebook</a>
<ul>
<ul>
-
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+
<li><a href="https://2012.igem.org/Team:UCSF/Bootcamp">Bootcamp</a></li>
-
    <li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/Safety">Safety</a></li>
+
                        <li><a herf="https://2013.igem.org/Team:UCSF/Notebook">Notebook</a></li>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/Collaboration">Collaboration</a></li>
+
                        <li><a class="drop"> Protocols</a>
 +
<ul>
 +
                                <li><a href="https://2012.igem.org/Team:UCSF/Protocols">General Protocols</a></li>
 +
                                <li><a href="https://2012.igem.org/Team:UCSF/Protocols2">Project Protocols</a></li>
 +
</ul>
 +
                        </li>
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-
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-
 
+
<li><a class="drop" id="outreachlink">Gallery</a>
-
<li><a href="https://2012.igem.org/Team:Johns_Hopkins-Software/PathogenDetection">Human Practices</a>
+
<ul>
<ul>
-
+
<li><a href="https://2012.igem.org/Team:UCSF/Video">Videos</a></li>
 +
<li><a href="https://2012.igem.org/Team:UCSF/Photos">Photos</a></li>
</ul>
</ul>
-
</li>
+
</li>
-
</div>
+
<li><a href="https://2013.igem.org" target="_blank">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</header>
 +
</body>
-
</div>
+
<div id="box1" align="justify">
-
</div>
+
</a>
 +
<h2>ABSTRACT</h2>
 +
<p>
 +
    The human race is growing fast. The world population is projected to top 10 billion by 2050. One response to the food supply needs of an increasing world population is creating better crop yield through crop survival. Pathogens and pests can cause catastrophic crop loss. Currently there is no widely used synthetic biological mechanism to help plants fight off bacterial pathogens and pests, so the main way large-scale growers protect crops is by using pesticides. We have engineered two possible solutions to address this problem.
 +
    </p>
 +
    <p>
 +
    In our first project, we have incorporated catalytically dead CAS9 (dCAS9) in conjunction with synthetic gRNAs complementary to pathogenic bacterial genes into a plasmid that naturally conjugates into neighboring bacterial cells. The specificity of the gRNAs will potentially allow us to target specific populations of bacterial pathogens in soil while leaving positive bacterial populations unharmed.
 +
    </p>
 +
    <p>
 +
    Our second project will provide plants with a sugar-mediated synthetic circuit that boosts a plant’s natural immune system and pesticide-producing capabilities in response to different levels of sugar a plant naturally produces in response to pests. The repression capabilities of dCAS9 will be used to regulate the synthetic circuit promoters that sense high or low sugar levels, reducing metabolic strain.
 +
    </p>
 +
    <p>
 +
    </p>
 +
    <p>
 +
    </p>
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<center>
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<h2>Special Thanks to our 2013 iGEM Team Sponsors!</h2>
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Team:UCSF/lily

From 2013.igem.org

ABSTRACT

The human race is growing fast. The world population is projected to top 10 billion by 2050. One response to the food supply needs of an increasing world population is creating better crop yield through crop survival. Pathogens and pests can cause catastrophic crop loss. Currently there is no widely used synthetic biological mechanism to help plants fight off bacterial pathogens and pests, so the main way large-scale growers protect crops is by using pesticides. We have engineered two possible solutions to address this problem.

In our first project, we have incorporated catalytically dead CAS9 (dCAS9) in conjunction with synthetic gRNAs complementary to pathogenic bacterial genes into a plasmid that naturally conjugates into neighboring bacterial cells. The specificity of the gRNAs will potentially allow us to target specific populations of bacterial pathogens in soil while leaving positive bacterial populations unharmed.

Our second project will provide plants with a sugar-mediated synthetic circuit that boosts a plant’s natural immune system and pesticide-producing capabilities in response to different levels of sugar a plant naturally produces in response to pests. The repression capabilities of dCAS9 will be used to regulate the synthetic circuit promoters that sense high or low sugar levels, reducing metabolic strain.

Special Thanks to our 2013 iGEM Team Sponsors!


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