Team:WashU StLouis

From 2013.igem.org

(Difference between revisions)

Latest revision as of 19:50, 8 August 2013

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.

Team WashU_StLouis

The production of a bio-fertilizer could benefit the agricultural industry by decreasing the need for energy intensive nitrogen fixation processes. The nif cluster of Cyanothece 51142 consists of 29 genes that construct and regulate a nitrogenase protein complex, which catalyzes the fixation of atmospheric nitrogen. This year, our iGEM team aims to harness the power of nif to produce ammonia in Escherichia coli. After synthesizing a nif- containing plasmid (34 kbp) using the DNA assembler method (Shao et al 2009) and transforming that plasmid into E. coli, our team tested for nitrogenase activity using the acetylene reduction assay. The transformed E. coli were then compared to wild-type under limited nitrogen conditions to check for a competitive advantage. Future tests will evaluate the expression of various nitrogenase subunits, such as nifD and nifK. Our team also aims to further characterize the promoter sequences of the Cyanothece 51142 nif cluster. Between the cysE and nifB genes, there is a 958bp uncharacterized, bidirectional promoter region of particular interest. We are currently using fluorescent reporters to identify key regions within this promoter sequence, and plan to test its function in various environmental conditions.

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

@@ Line 2: / Line 2: @@
 <html>
 <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
 <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
@@ Line 20: / Line 21: @@
 {|align="justify"
-|Project Overview
-     The production of a bio-fertilizer could benefit the agricultural industry by decreasing the need for energy intensive nitrogen fixation processes. The ''nif'' cluster of ''Cyanothece'' 51142 consists of 29 genes that construct and regulate a nitrogenase protein complex, which catalyzes the fixation of atmospheric nitrogen. This year, our iGEM team aims to harness the power of ''nif'' to produce ammonia in ''Escherichia coli''. After synthesizing a ''nif''-containing plasmid (34 kbp) using the DNA assembler method (Shao et al 2009) and transforming that plasmid into ''E. coli'', our team tested for nitrogenase activity using the acetylene reduction assay. The transformed ''E. coli'' were then compared to wild-type under limited nitrogen conditions to check for a competitive advantage. Future tests will evaluate the expression of various nitrogenase subunits, such as ''nifD'' and ''nifK''.
-     Our team also aims to further characterize the promoter sequences of the Cyanothece 51142 ''nif'' cluster. Between the ''cysE'' and ''nifB'' genes, there is a 958bp uncharacterized, bidirectional promoter region of particular interest. We are currently using fluorescent reporters to identify key regions within this promoter sequence, and plan to test its function in various environmental conditions.
-|[[Image:WashU_StLouis_logo.png|200px|right|frame]]
-|-
-|
-''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
-|[[Image:WashU_StLouis_team.png|right|frame|Your team picture]]
-|-
-|
 |align="center"|[[Team:WashU_StLouis | Team WashU_StLouis]]
+The production of a bio-fertilizer could benefit the agricultural industry by decreasing the need for energy intensive nitrogen fixation processes. The ''nif'' cluster of ''Cyanothece'' 51142 consists of 29 genes that construct and regulate a nitrogenase protein complex, which catalyzes the fixation of atmospheric nitrogen. This year, our iGEM team aims to harness the power of ''nif'' to produce ammonia in ''Escherichia coli''. After synthesizing a ''nif''- containing plasmid (34 kbp) using the DNA assembler method (Shao et al 2009) and transforming that plasmid into ''E. coli'', our team tested for nitrogenase activity using the acetylene reduction assay. The transformed ''E. coli'' were then compared to wild-type under limited nitrogen conditions to check for a competitive advantage. Future tests will evaluate the expression of various nitrogenase subunits, such as ''nifD'' and ''nifK''.
+Our team also aims to further characterize the promoter sequences of the Cyanothece 51142 ''nif'' cluster. Between the ''cysE'' and ''nifB'' genes, there is a 958bp uncharacterized, bidirectional promoter region of particular interest. We are currently using fluorescent reporters to identify key regions within this promoter sequence, and plan to test its function in various environmental conditions.
 |}
 <!--- The Mission, Experiments --->
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+{| style="color:#1b2c8a;background-color:http://4.bp.blogspot.com/-ZcYLHi0RNNc/UEBlZivLzyI/AAAAAAAACYs/RGa97yyjW7Y/s1600/green_website_background_pattern.jpg;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 !align="center"|[[Team:WashU_StLouis|Home]]
 !align="center"|[[Team:WashU_StLouis/Team|Team]]