Team:Glendale CC AZ/Protocols/SurivalGrowth

From 2013.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Glendale_CC_AZ/test}}
+
{{:Team:Glendale_CC_AZ/main.css}}  
 +
<html>
 +
<head>
 +
<link
 +
href="http://fonts.googleapis.com/css?family=PT+Serif:400,700,400italic,700italic"
 +
rel="stylesheet" type="text/css">
 +
<link
 +
href="http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,700,300italic"
 +
rel="stylesheet" type="text/css">
 +
<meta charset="UTF-8">
 +
<style type="text/css">
 +
body {
 +
  margin: 0;
 +
  padding: 0;
 +
  background: rgb(230,230,230);
 +
 
 +
  color: rgb(50,50,50);
 +
  font-family: 'Open Sans', sans-serif;
 +
  font-size: 112.5%;
 +
  line-height: 1.6em;
 +
}
-
== Survival Growth Assay ==
+
/* ================ The Timeline ================ */
 +
.timeline {
 +
  position: relative;
 +
  width: 660px;
 +
  margin: 0 auto;
 +
  margin-top: 20px;
 +
  padding: 1em 0;
 +
  list-style-type: none;
 +
}
 +
p list {
 +
  text-align: left;
 +
}
-
== Materials ==
+
.timeline:before {
 +
  position: absolute;
 +
  left: 50%;
 +
  top: 0;
 +
  content: ' ';
 +
  display: block;
 +
  width: 6px;
 +
  height: 100%;
 +
  margin-left: -3px;
 +
  background: rgb(80,80,80);
 +
  background: -moz-linear-gradient(top, rgba(80,80,80,0) 0%, rgb(80,80,80) 8%, rgb(80,80,80) 92%, rgba(80,80,80,0) 100%);
 +
  background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,rgba(30,87,153,1)), color-stop(100%,rgba(125,185,232,1)));
 +
  background: -webkit-linear-gradient(top, rgba(80,80,80,0) 0%, rgb(80,80,80) 8%, rgb(80,80,80) 92%, rgba(80,80,80,0) 100%);
 +
  background: -o-linear-gradient(top, rgba(80,80,80,0) 0%, rgb(80,80,80) 8%, rgb(80,80,80) 92%, rgba(80,80,80,0) 100%);
 +
  background: -ms-linear-gradient(top, rgba(80,80,80,0) 0%, rgb(80,80,80) 8%, rgb(80,80,80) 92%, rgba(80,80,80,0) 100%);
 +
  background: linear-gradient(to bottom, rgba(80,80,80,0) 0%, rgb(80,80,80) 8%, rgb(80,80,80) 92%, rgba(80,80,80,0) 100%);
 +
 
 +
  z-index: 5;
 +
}
 +
 
 +
.timeline li {
 +
  padding: 1em 0;
 +
}
 +
 
 +
.timeline li:after {
 +
  content: "";
 +
  display: block;
 +
  height: 0;
 +
  clear: both;
 +
  visibility: hidden;
 +
}
 +
 
 +
.direction-l {
 +
  position: relative;
 +
  width: 300px;
 +
  float: left;
 +
  text-align: right;
 +
}
 +
 
 +
.direction-r {
 +
  position: relative;
 +
  width: 300px;
 +
  float: right;
 +
}
 +
 
 +
.flag-wrapper {
 +
  position: relative;
 +
  display: inline-block;
 +
 
 +
  text-align: center;
 +
}
 +
 
 +
.flag {
 +
  position: relative;
 +
  display: inline;
 +
  background: rgb(248,248,248);
 +
  padding: 6px 10px;
 +
  border-radius: 5px;
 +
 
 +
  font-weight: 600;
 +
  text-align: left;
 +
}
 +
 
 +
.direction-l .flag {
 +
  -webkit-box-shadow: -1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
  -moz-box-shadow: -1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
  box-shadow: -1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
}
 +
 
 +
.direction-r .flag {
 +
  -webkit-box-shadow: 1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
  -moz-box-shadow: 1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
  box-shadow: 1px 1px 1px rgba(0,0,0,0.15), 0 0 1px rgba(0,0,0,0.15);
 +
}
 +
 
 +
.direction-l .flag:before,
 +
.direction-r .flag:before {
 +
  position: absolute;
 +
  top: 50%;
 +
  right: -40px;
 +
  content: ' ';
 +
  display: block;
 +
  width: 12px;
 +
  height: 12px;
 +
  margin-top: -10px;
 +
  background: #fff;
 +
  border-radius: 10px;
 +
  border: 4px solid rgb(255,80,80);
 +
  z-index: 10;
 +
}
 +
 
 +
.direction-r .flag:before {
 +
  left: -40px;
 +
}
 +
 
 +
.direction-l .flag:after {
 +
  content: "";
 +
  position: absolute;
 +
  left: 100%;
 +
  top: 50%;
 +
  height: 0;
 +
  width: 0;
 +
  margin-top: -8px;
 +
  border: solid transparent;
 +
  border-left-color: rgb(248,248,248);
 +
  border-width: 8px;
 +
  pointer-events: none;
 +
}
 +
 
 +
.direction-r .flag:after {
 +
  content: "";
 +
  position: absolute;
 +
  right: 100%;
 +
  top: 50%;
 +
  height: 0;
 +
  width: 0;
 +
  margin-top: -8px;
 +
  border: solid transparent;
 +
  border-right-color: rgb(248,248,248);
 +
  border-width: 8px;
 +
  pointer-events: none;
 +
}
 +
 
 +
.time-wrapper {
 +
  display: inline;
 +
 
 +
  line-height: 1em;
 +
  font-size: 0.66666em;
 +
  color: rgb(250,80,80);
 +
  vertical-align: middle;
 +
}
 +
 
 +
.direction-l .time-wrapper {
 +
  float: left;
 +
}
 +
 
 +
.direction-r .time-wrapper {
 +
  float: right;
 +
}
 +
 
 +
.time {
 +
  display: inline-block;
 +
  padding: 4px 6px;
 +
  background: rgb(248,248,248);
 +
}
 +
 
 +
.desc {
 +
  margin: 1em 0.75em 0 0;
 +
 
 +
  font-size: 0.77777em;
 +
  font-style: italic;
 +
  line-height: 1.5em;
 +
}
 +
 
 +
.direction-r .desc {
 +
  margin: 1em 0 0 0.75em;
 +
}
 +
 
 +
/* ================ Timeline Media Queries ================ */
 +
 
 +
@media screen and (max-width: 660px) {
 +
 
 +
.timeline {
 +
width: 100%;
 +
padding: 4em 0 1em 0;
 +
}
 +
 
 +
.timeline li {
 +
padding: 2em 0;
 +
}
 +
 
 +
.direction-l,
 +
.direction-r {
 +
float: none;
 +
width: 100%;
 +
 
 +
text-align: center;
 +
}
 +
 
 +
.flag-wrapper {
 +
text-align: center;
 +
}
 +
 
 +
.flag {
 +
background: rgb(255,255,255);
 +
z-index: 15;
 +
}
 +
 
 +
.direction-l .flag:before,
 +
.direction-r .flag:before {
 +
  position: absolute;
 +
 
 +
  top: -30px;
 +
left: 50%;
 +
content: ' ';
 +
display: block;
 +
width: 12px;
 +
height: 12px;
 +
margin-left: -9px;
 +
background: #fff;
 +
border-radius: 10px;
 +
border: 4px solid rgb(255,80,80);
 +
z-index: 10;
 +
}
 +
 
 +
.direction-l .flag:after,
 +
.direction-r .flag:after {
 +
content: "";
 +
position: absolute;
 +
left: 50%;
 +
top: -8px;
 +
height: 0;
 +
width: 0;
 +
margin-left: -8px;
 +
border: solid transparent;
 +
border-bottom-color: rgb(255,255,255);
 +
border-width: 8px;
 +
pointer-events: none;
 +
}
 +
 
 +
.time-wrapper {
 +
display: block;
 +
position: relative;
 +
margin: 4px 0 0 0;
 +
z-index: 14;
 +
}
 +
 
 +
.direction-l .time-wrapper {
 +
float: none;
 +
}
 +
 
 +
.direction-r .time-wrapper {
 +
float: none;
 +
}
 +
 
 +
.desc {
 +
position: relative;
 +
margin: 1em 0 0 0;
 +
padding: 1em;
 +
background: rgb(245,245,245);
 +
-webkit-box-shadow: 0 0 1px rgba(0,0,0,0.20);
 +
-moz-box-shadow: 0 0 1px rgba(0,0,0,0.20);
 +
box-shadow: 0 0 1px rgba(0,0,0,0.20);
 +
 +
  z-index: 15;
 +
}
 +
 
 +
.direction-l .desc,
 +
.direction-r .desc {
 +
position: relative;
 +
margin: 1em 1em 0 1em;
 +
padding: 1em;
 +
 +
  z-index: 15;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (min-width: 400px ?? max-width: 660px) {
 +
 
 +
.direction-l .desc,
 +
.direction-r .desc {
 +
margin: 1em 4em 0 4em;
 +
}
 +
 
 +
} 
 +
* {
 +
-webkit-box-sizing: border-box;
 +
-moz-box-sizing: border-box;
 +
box-sizing: border-box;
 +
}
 +
 
 +
html {
 +
font-size:50%;
 +
-webkit-font-smoothing: antialiased;
 +
}
 +
 
 +
body {
 +
font-family: "Source Sans Pro";
 +
font-size:1.4rem;
 +
line-height: 2.3rem;
 +
color:#444;
 +
background: #fff;
 +
margin:0;
 +
}
 +
 
 +
a {
 +
color: #06c;
 +
transition: color .3s;
 +
}
 +
 
 +
a:hover {
 +
color: #012c57;
 +
}
 +
 
 +
h1, h2, h3, h4, h5, h6, h7 {
 +
font-family: "Futura";
 +
line-height:2.6rem;
 +
font-weight:normal;
 +
color: #111;
 +
}
 +
 
 +
.content-area {
 +
width:850px;
 +
padding:2rem;
 +
margin:auto;
 +
background: #f9f9f9;
 +
}
 +
 
 +
.content-area h1 {
 +
font-size: 2.7rem;
 +
}
 +
 
 +
.content-area h2 {
 +
font-size: 2.2rem;
 +
}
 +
 
 +
.content-area h3 {
 +
font-size: 2.7rem;
 +
}
 +
 
 +
.content-area h4 {
 +
font-size: 4.0rem;
 +
}
 +
 
 +
.content-area h5 {
 +
font-size: 1.4rem;
 +
}
 +
 
 +
.content-area h6 {
 +
font-size: 1.2rem;
 +
}
 +
 
 +
.content-area aside {
 +
float:right;
 +
width:50%;
 +
padding:2rem;
 +
 
 +
.blockquote {
 +
font-family: Georgia, serif;
 +
font-size: 18px;
 +
font-style: italic;
 +
width: 500px;
 +
margin: 0.25em 0;
 +
padding: 0.35em 40px;
 +
line-height: 1.45;
 +
position: relative;
 +
color: #383838;
 +
}
 +
 
 +
.blockquote:before {
 +
display: block;
 +
padding-left: 10px;
 +
content: "\201C";
 +
font-size: 80px;
 +
position: absolute;
 +
left: -20px;
 +
top: -20px;
 +
color: #7a7a7a;
 +
}
 +
 
 +
.blockquote cite {
 +
color: #999999;
 +
font-size: 14px;
 +
display: block;
 +
margin-top: 5px;
 +
}
 +
 
 +
.blockquote cite:before {
 +
content: "\2014 \2009";
 +
}
 +
 
 +
 
 +
</style>
 +
<title></title>
 +
</head>
 +
<body>
 +
<div class="content-area"> <article>
 +
</article>
 +
<img style="border: 0px solid ; width: 50px; height: 40px;" alt="iGEM" src="http://s21.postimg.org/ff5nkjy9v/IGEM_basic_Logo_stylized.png" align="left">
 +
<h4>Glendale Community College Arizona<img
 +
style="width: 200px; height: 58px;" alt="GCC"
 +
src="https://static.igem.org/mediawiki/2013/f/f1/Gcclogo.gif" align="right">
 +
</h4>
 +
 
 +
 
 +
 +
 
 +
 
 +
 
 +
<link href='http://fonts.googleapis.com/css?family=Open+Sans:400,300,300italic,400italic,600,600italic,700,700italic' rel='stylesheet' type='text/css'>
 +
 
 +
 
 +
  <h2><p>Protocols</p></h2><img style="border: 0px solid ; width: 400px; height: 200px;" alt="iGEM" src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" align="right">
 +
 
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve  Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/NaCl">NaCl Growth Curve Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth">Survival Growth  Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/RestrictionDigest">Restriction Digest</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/DNAIsolation">DNA Isolation</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Bioinformatics">Bioinformatics</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation">Ligation</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Transformation">Transformation</a></p>
 +
 
 +
 
 +
<!--/.content-area-->
 +
</body>
 +
</html>
 +
 
 +
==== Survival Growth Assay ====
 +
 
 +
 
 +
==== Materials ====
     -P200 micropipette
     -P200 micropipette
Line 13: Line 457:
     -Spreader
     -Spreader
     -20mL ethanol (to sterilize spreader)
     -20mL ethanol (to sterilize spreader)
-
     -Incubator set to 37 degrees celcius.
+
     -Incubator set to 37°C.
-
== Procedure ==
+
==== Procedure ====
1. Label all plates (Keep upside down to avoid condensation on agar)
1. Label all plates (Keep upside down to avoid condensation on agar)
Line 28: Line 472:
5. Spread bacteria-LB solution all over plate.
5. Spread bacteria-LB solution all over plate.
-
6. Incubate at 37 degrees celcius for up to 24 hours.
+
6. Incubate at 37°C for up to 24 hours.

Latest revision as of 02:34, 28 September 2013

iGEM

Glendale Community College ArizonaGCC

Protocols

iGEM Growth Curve Assay

NaCl Growth Curve Assay

Survival Growth Assay

Alkaline Lysis Plasmid Miniprep

Restriction Digest

DNA Isolation

Bioinformatics

Ligation

Transformation

Survival Growth Assay

Materials 

   -P200 micropipette
   -10µl bacteria
   -90µl LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37°C.


Procedure

1. Label all plates (Keep upside down to avoid condensation on agar)

2. Pipette LB media into tube containing bacteria; aspirate.

3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.

4. Briefly flame the spreader and let cool momentarily.

5. Spread bacteria-LB solution all over plate.

6. Incubate at 37°C for up to 24 hours.

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth"