Team:DTU-Denmark/Methods/Calibrating Electrodes
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==Calibration of NO and N<sub>2</sub>O electrodes/probes== | ==Calibration of NO and N<sub>2</sub>O electrodes/probes== | ||
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Latest revision as of 18:09, 15 August 2013
Calibration of electrodes
Calibration of NO and N2O electrodes/probes
- Cleaning the probes
- Prepare 96% ethanol solution
- Prepare 0.01M HCl solution
- Put both probes in ethanol solution for 1 minute
- Put both probes in HCl solution for 1 minute
- Rinse with demineralized water
- Calibration
- Make standard solution NO
- Make 200 ml solution 1 M NaOH
- Make it anoxic by bubbling with N2 for 5 minutes
- Make MQ water anoxic
- Bubble 5% NO solution through NaOH solution
- Convey towards the water flask for 20 minutes
- Make standard solution of N2O
- Make solution anoxic by bubbling 5 minutes with N2
- Flush with N2O for 20 minutes.
- Procedure
- Pure 200 ml of DM minimal medium in the bottle
- Adjust the temperature at around 37oC by using a magnetic stirrer
- Adjust both of the probes in the lid of the bottle
- Connect the electrodes with the microsensors
- Make the solution anoxic by bubbling with N2 for 10 minutes
- Start by calibrating N2O sensor (the adding points are the concentration of N2O in mg/L)
- Add the first point “0” on the sensor
- Spike 0.5 ml of N2O in 200 ml and add point “60.35”
- Spike 0.5 ml of N2O and add point “120.7”
- Spike 0.5 ml of N2O and add point “181.06”
- Spike 0.5 ml of N2O and add point “2241.41”
- Spike 1 ml of N2O and add point “362.11”
- Spike 1 ml of N2O and add point “482.81”
- Then apply the calibration for the sensor
- Continue by calibrating NO sensor (the adding points are the concentration of NO in mg/L)
- Add the first point “0” on the sensor
- Spike 1 ml of NO in 200 ml and add point “0.47”
- Spike 1 ml of NO and add point “0.94”
- Spike 1 ml of NO and add point “1.4”
- Spike 1 ml of NO and add point “1.86”
- Spike 1 ml of NO and add point “2.31”
- Then apply the calibration for the sensor