Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
m
 
(113 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
-
 
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
-
<html>
+
<head>
<head>
-
<title>iGEM Team : KAIST_Korea</title>
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
+
<meta name="generator" content="MediaWiki 1.16.5" />
-
<meta name="description" content="" />
+
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<meta name="keywords" content="" />
+
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<noscript>
+
<link rel="shortcut icon" href="/favicon.ico" />
-
<link rel="stylesheet" href="css/5grid/core.css" />
+
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
-
<link rel="stylesheet" href="css/5grid/core-desktop.css" />
+
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
-
<link rel="stylesheet" href="css/5grid/core-1200px.css" />
+
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-
<link rel="stylesheet" href="css/5grid/core-noscript.css" />
+
<meta name="viewport" content="width=device-width, initial-scale=1.0 minimum-scale=1, maximum-scale=1, user-scalable=yes, target-densitydpi=medium-dpi" />
-
<link rel="stylesheet" href="css/style.css" />
+
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic' rel='stylesheet' type='text/css'>
-
<link rel="stylesheet" href="css/style-desktop.css" />
+
<title>Team:KAIST Korea/Project Background - 2013.igem.org</title>
-
</noscript>
+
<style type="text/css" media="screen, projection">/*<![CDATA[*/
-
<script src="css/5grid/jquery.js"></script>
+
@import "/wiki/skins/common/shared.css?270";
-
<script src="css/5grid/init.js?use=mobile,desktop,1000px&amp;mobileUI=1&amp;mobileUI.theme=none"></script>
+
@import "/wiki/skins/igem/main.css?270";
-
<!--[if IE 9]><link rel="stylesheet" href="css/style-ie9.css" /><![endif]-->
+
#bar{z-index : 5;
-
</head><body>
+
}
 +
#menu,
 +
#menu ul,
 +
#menu li,
 +
#menu a {
 +
margin: 0;
 +
padding-left: 40;
 +
border: none;
 +
outline: none;
 +
}
 +
.menu {
 +
height: 50px;
 +
weight: 100%;
 +
padding-left: 30%;
 +
        z-index: 2;
 +
background: #000000;
 +
}
 +
 +
.menu li {
 +
position: relative;
 +
list-style: none;
 +
margin: 0px auto 0 auto;
 +
float: left;
 +
display: block;
 +
height: 50px;
 +
}
 +
 +
/* Links */
 +
 +
.menu li a {
 +
margin:0px auto;
 +
display: block;
 +
padding: 0 14px;
 +
margin: 6px 0;
 +
 +
text-decoration: none;
 +
-
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
+
-
<script type="text/javascript" src="jquery.easing.1.3.js"></script>
+
font-family: Helvetica, Arial, sans-serif;
 +
font-weight: bold;
 +
font-size: 20px;
 +
 +
color: #353535;
 +
 +
 +
-webkit-transition: color .2s ease-in-out;
 +
-moz-transition: color .2s ease-in-out;
 +
-o-transition: color .2s ease-in-out;
 +
-ms-transition: color .2s ease-in-out;
 +
transition: color .2s ease-in-out;
 +
}
 +
 +
.menu li:first-child a { border-left: none; }
 +
.menu li:last-child a{ border-right: none; }
 +
 +
.menu li:hover > a { color: #F29661; }
 +
 +
/* Sub Menu */
 +
 +
.menu ul {
 +
position: absolute;
 +
top: 50px;
 +
 +
left: 0px;
 +
 +
padding-right: 10px;
 +
 +
opacity: 0;
 +
 +
background: #1f2024;
 +
 +
-webkit-border-radius: 0 0 5px 5px;
 +
-moz-border-radius: 0 0 5px 5px;
 +
border-radius: 0 0 5px 5px;
 +
 +
-webkit-transition: opacity .25s ease .1s;
 +
-moz-transition: opacity .25s ease .1s;
 +
-o-transition: opacity .25s ease .1s;
 +
-ms-transition: opacity .25s ease .1s;
 +
transition: opacity .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul { opacity: 1; }
 +
 +
.menu ul li {
 +
height: 0;
 +
overflow: hidden;
 +
padding: 0px;
 +
 +
-webkit-transition: height .25s ease .1s;
 +
-moz-transition: height .25s ease .1s;
 +
-o-transition: height .25s ease .1s;
 +
-ms-transition: height .25s ease .1s;
 +
transition: height .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul li {
 +
height: 40px;
 +
overflow: visible;
 +
padding: 0;
 +
}
 +
 +
.menu ul li a {
 +
width: 100px;
 +
padding: 4px 0 4px 40px;
 +
margin: 0;
 +
 +
border: none;
 +
border-bottom: 1px solid #353539;
 +
}
 +
 +
.menu ul li:last-child a { border: none; }
 +
 +
/* Icons */
 +
 +
.menu a.documents { background: url(docs.png) no-repeat 6px center; }
 +
.menu a.messages { background: url(bubble.png) no-repeat 6px center; }
 +
.menu a.signout { background: url(arrow.png) no-repeat 6px center; }
 +
 +
 +
ul.menu { margin: 0px auto 0 auto; }
 +
</style>
 +
 
 +
<style type="text/css">
 +
 +
/* Cross-Browsing */
 +
html,body,div,ul,ol,li,dl,dt,dd,h1,h2,h3,h4,h5,h6,pre,form,p,blockquote,fieldset,input,hr {margin:0; padding:0;}
 +
h1,h2,h3,h4,h5,h6,pre,code,address,caption,cite,code,em,strong,th {font-size:1em; font-weight:normal; font-style:normal;}
 +
ol, ul, li { list-style:none; }
 +
fieldset,img,hr {border:none;}
 +
caption,th {text-align:left;}
 +
table {border-collapse:collapse; border-spacing:0;}
 +
td {vertical-align:top;}
 +
strong { font-weight:bold ;}
 +
em { font-style:italic; }
 +
a img { border:none; }
 +
q:before, q:after { content:''}
 +
 +
html { height: 100%; }
 +
 +
 +
body
 +
{
 +
background-image: url('https://static.igem.org/mediawiki/2012/6/61/Top_section_expand_KAIST.png'),url('https://static.igem.org/mediawiki/2012/9/98/PATTERN_diagonal.png');
 +
background-repeat: repeat-x,repeat;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
 +
 +
#content
 +
{
 +
border: none;
 +
position: relative;
 +
top: 0;
 +
background-color: transparent;
 +
padding: 0;
 +
width: 100%;
 +
}
 +
 +
#bodyContent p
 +
{
 +
display: none;
 +
}
 +
 +
#globalWrapper
 +
{
 +
clear: both;
 +
width: 100%;
 +
                z-index: 1;
 +
position: relative;
 +
top:0px;
 +
margin: auto;
 +
font-size: 10px;
 +
}
 +
 +
#contentSub { display: none; }
 +
#siteSub { display:none; }
 +
 +
#search-controls {
 +
left: 20px;
 +
width: auto;
 +
        z-index: 100;
 +
        position: relative;
 +
        float: right;
 +
        top: -10px;
 +
        right: 0;
 +
        height: auto;
 +
        clear: both;
 +
}
 +
 +
input.searchButton
 +
{
 +
position: relative;
 +
font-size: 1em;
 +
font-family: "Arial";
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 +
#searchInput
 +
{
 +
background-color: transparent;
 +
z-index: 100;
 +
color: #181818;
 +
font: Arial;
 +
text-align: center;
 +
 +
background: url('https://static.igem.org/mediawiki/2012/6/6e/Kaist2012_head_searchbar.png') no-repeat;
 +
background-position: 0px 0px;
 +
vertical-align: middle;
 +
 +
width: 184px;
 +
height: 22px;
 +
border: none;
 +
}
 +
 +
#searchGoButton
 +
{
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/7/78/Kaist2012_head_arrow.png') no-repeat;
 +
background-position: left 0px;
 +
        z-index: 100;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
#mw-searchButton
 +
{
 +
position: relative;
 +
left: -14px;
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
 +
                z-index: 100;
 +
background-position: left 0px;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
.firstHeading { display:none; }
 +
 +
#p-logo
 +
{
 +
display: none;
 +
position: relative;
 +
top: 0px;
 +
z-index: 0;
 +
height: 1px;
 +
width: 10px;
 +
overflow: hidden;
 +
}
 +
 +
#p-logo a { color: black; }
 +
 +
img[src*="/wiki/skins/common/images/wiki.png"]
 +
{
 +
    visibility: hidden;
 +
    top: -100px;
 +
    width: 10px;
 +
    height: 0px;
 +
margin-top: 20px;
 +
    background: transparent;
 +
}
 +
 +
 +
 +
#menubar
 +
{
 +
font-size: 10px;
 +
font-family: "GothamXNarrow-Bold", "Helvetica";
 +
position: relative;
 +
float: left;
 +
width: auto;
 +
top: 36px;
 +
        z-index: 100;
 +
}
 +
 +
#menubar:hover{ background-color: #181818; }
 +
 +
.left-menu { margin-top: 0px; left: 0; }
 +
.left-menu ul { border: none;}
 +
.right-menu { margin-top: 0px; right: 0; float: right !important;}
 +
.right-menu ul { border: none; }
 +
.right-menu ul a { background-color: transparent; }
 +
.left-menu li a { color: #615d5a; }
 +
.left-menu li a:visited { color: #615d5a; }
 +
.left-menu li a:hover { color: #ffffff; }
 +
.left-menu li a:active{ color: #cccccc; font-weight: bold; }
 +
.right-menu li a { color: #f6f5c8; }
 +
.right-menu li a:visited { color: #f6f5c8; }
 +
.right-menu li a:hover { color: #ffffff; text-decoration: none; }
 +
.right-menu li a:active { color: #cccccc; text-decoration: none;font-weight: bold; }
 +
 +
#menubar li {
 +
 +
        margin: 0px;
 +
        word-spacing:-1px;
 +
}
 +
 +
#catlinks {
 +
position: relative;
 +
top: 5px;
 +
left: -5px;
 +
width: 1034px;
 +
border: none;
 +
background-color: transparent;
 +
padding-top: 5px;
 +
clear: both;
 +
}
 +
 +
#footer-box {display:none;}
 +
#footer {display:none;}
 +
#footer li a { color: #000000; }
 +
#footer img { margin: 10px; border: none; }
 +
#footer li a:visited {color: #000000;}
 +
#footer li a:hover { color: #ababab; text-decoration: none; }
 +
#footer li a:active{ color: #ababab; text-decoration: none; font-weight: bold}
 +
        </style>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic);
 +
ul.oe_menu{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
 +
 +
}
 +
 +
ul.oe_menu > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#aaa;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu > li > a:hover,
 +
ul.oe_menu > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
.oe_wrapper ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
ul.oe_menu div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu div ul li a{
 +
text-decoration:none;
 +
color:#cbcbcb;
 +
padding:2px 2px 2px 4px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
ul.oe_menu div ul.oe_full{
 +
width:100%;
 +
}
 +
ul.oe_menu div ul li a:hover{
 +
background:#8583bf;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
ul.oe_menu li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
ul.oe_menu2{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu2 > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
 +
}
 +
 +
ul.oe_menu2 > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#eee;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu2 > li > a:hover,
 +
ul.oe_menu2 > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
 +
.oe_wrapper2 ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
 +
ul.oe_menu2 div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu2 div ul li a{
 +
text-decoration:none;
 +
color:#ffffff;
 +
padding:2px 2px 2px 2px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
 +
ul.oe_menu2 div ul.oe_full2{
 +
width:100%;
 +
}
 +
 +
ul.oe_menu2 div ul li a:hover{
 +
background:#d37c62;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
 +
ul.oe_menu2 li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
#main
 +
{
 +
min-height: 100%;
 +
width: 1024px;
 +
margin: 0 auto;
 +
background-color: transparent;
 +
background-image:url("https://static.igem.org/mediawiki/2012/d/da/Kaist_home_background_y_extended.png");
 +
background-repeat:repeat-y;
 +
}
 +
 +
#main #main-img
 +
{
 +
position: absolute;
 +
width: 1024px;
 +
top: -30px;
 +
z-index:0;
 +
}
 +
 +
#main a #main-logo
 +
{
 +
position: absolute;
 +
top: -30px;
 +
width: 336px;
 +
z-index:1;
 +
}
 +
 +
#main #top-img
 +
{
 +
position: relative;
 +
top: -5px;
 +
        left:294px;
 +
width:729px;
 +
height: 250px;
 +
z-index: 0;
 +
opacity:0.9;
 +
}
 +
 +
div .oe_wrapper{
 +
position:absolute;
 +
z-index:3;
 +
top:275px;
 +
}
 +
 +
div .oe_wrapper2{
 +
position:absolute;
 +
z-index:2;
 +
top:358px;
 +
}
 +
 +
div .iGEMLogo{
 +
position:absolute;
 +
z-index:4;
 +
bottom: 80px;
 +
padding : 0 0 0 10px;
 +
}
 +
 +
#menucontent
 +
{
 +
        position: relative;
 +
        background-color:transparent;
 +
        width:690px;
 +
        left:320px;
 +
        top:-75px;
 +
        z-index:2;
 +
        text-align: right;
 +
        opacity:0.8;
 +
}
 +
 +
#top-img-description
 +
{
 +
font-size:25px;
 +
font-family: 'Gloria Hallelujah', cursive;
 +
}
 +
 +
#top-img-description-box
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        top:-115px;
 +
left: 350px;
 +
        z-index:3;
 +
        height:15px;
 +
}
 +
 +
#horizontal-line
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        width:732px;
 +
height:3px;
 +
        left:292px;
 +
        top:-90px;
 +
        z-index:2;
 +
}
 +
div .contact-us{
 +
position: absolute;
 +
z-index:2;
 +
top:450px;
 +
padding:4px 0px 0px 10px;
 +
width:268px;
 +
color:white;
 +
font-weight:bold;
 +
}
 +
 +
div .contact-us #contact-us-information{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
}
 +
 +
div .contact-us #contact-us-information a:hover{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:#fcf967;
 +
font-weight:bold;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-information a{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-title{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:22px;
 +
line-height:170%;
 +
color:#dddddd;
 +
height:30px;
 +
}
 +
 +
</style>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Source+Sans+Pro:300,400,300italic:latin' ] }
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })(); </script>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 +
<meta http-equiv="imagetoolbar" content="no" />
 +
<script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2013.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:KAIST_Korea/Project",
 +
wgTitle="Team:KAIST Korea/Project",
 +
wgAction="view",
 +
wgArticleId=9665,
 +
wgIsArticle=true,
 +
wgUserName="Springkjw",
 +
wgUserGroups=["*", "user", "autoconfirmed"],
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=297918,
 +
wgVersion="1.16.0",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2013.igem.org",
 +
wgCategories=[],
 +
wgMWSuggestTemplate="https://2013.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 +
wgDBname="2013_igem_org",
 +
wgSearchNamespaces=[0],
 +
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[],
 +
wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"};
 +
</script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script src="/wiki/skins/common/ajaxwatch.js?270"></script>
 +
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;smaxage=0&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
<!-- jQuery Javascript -->
 +
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
 +
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
 +
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
 +
 
 +
 
 +
<link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Open+Sans:600italic,400,800:latin' ]|[ 'Gloria+Hallelujah::latin' ]}
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })();
 +
  </script>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800);
 +
 +
#kaistcontent
 +
{
 +
position: relative;
 +
background-color:transparent;
 +
width:690px;
 +
font-family: 'Open Sans', sans-serif;
 +
font-size:11pt;
 +
line-height:140%;
 +
padding: 0px 15px 0px 15px;
 +
left:300px;
 +
top:-70px;
 +
z-index: 2;
 +
}
 +
 +
#kaistcontent #tab
 +
{
 +
width:200px;
 +
height:30px;
 +
position:relative;
 +
background:#f2f3f3;
 +
padding:5px 30px 5px 30px;
 +
border:1px solid #f4f4f4;
 +
margin : 0 20px 0 20px;
 +
}
 +
 +
 +
 +
#kaistcontent #starter-grad
 +
{
 +
width:17px;
 +
height:100px;
 +
float:left;
 +
margin:0px 15px 2px 0px;
 +
}
 +
 +
#kaistcontent #starter
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
font-size:25pt;
 +
color: #08185d;
 +
font-style:italic
 +
padding:0px 0px 0px 10px;
 +
}
 +
 +
#kaistcontent #sub-title
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#848383;
 +
font-size: 15pt;
 +
line-height:100%;
 +
font-weight:bold;
 +
font-style:italic;
 +
}
 +
 +
#kaistcontent h1
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#5c5858;
 +
font-weight:bold;
 +
font-size:24pt;
 +
line-height:110%;
 +
}
 +
 +
#kaistcontent ul
 +
{
 +
list-style-type:disc;
 +
padding: 0px 0px 0px 30px;
 +
 +
}
 +
 +
#kaistcontent li
 +
{
 +
list-style-type:decimal;
 +
}
 +
 +
#kaistcontent a { 
 +
color:#5a5b5e; 
 +
 +
 +
#kaistcontent a:hover{ 
 +
    color:#94969a; 
 +
 +
 +
 +
#kaistcontent #little
 +
{
 +
padding: 0px 0px 0px 30px;
 +
line-height:140%;
 +
}
 +
#kaistcontent #little2
 +
{
 +
padding: 0px 0px 0px 15px;
 +
line-height:140%;
 +
}
 +
 +
#kaistcontent #sub-sub-title
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#848383;
 +
font-weight:bold;
 +
font-size:14pt;
 +
line-height:110%;
 +
}
 +
 +
</style>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
         <script type="text/javascript">
         <script type="text/javascript">
             $(function() {
             $(function() {
-
/**
+
var $oe_menu = $('#oe_menu');
-
* for each menu element, on mouseenter,
+
var $oe_menu_items = $oe_menu.children('li');
-
* we enlarge the image, and show both sdt_active span and
+
-
* sdt_wrap span. If the element has a sub menu (sdt_box),
+
                $oe_menu_items.bind('mouseenter',function(){
-
* then we slide it - if the element is the last one in the menu
+
var $this = $(this);
-
* we slide it to the left, otherwise to the right
+
$this.addClass('slided selected');
-
*/
+
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
-
                $('#sdt_menu > li').bind('mouseenter',function(){
+
$oe_menu_items.not('.slided').children('div').hide();
-
var $elem = $(this);
+
$this.removeClass('slided');
-
$elem.find('img')
+
-
.stop(true)
+
-
.animate({
+
-
'width':'170px',
+
-
'height':'170px',
+
-
'left':'0px'
+
-
},400,'easeOutBack')
+
-
.andSelf()
+
-
.find('.sdt_wrap')
+
-
    .stop(true)
+
-
.animate({'top':'140px'},500,'easeOutBack')
+
-
.andSelf()
+
-
.find('.sdt_active')
+
-
    .stop(true)
+
-
.animate({'height':'170px'},300,function(){
+
-
var $sub_menu = $elem.find('.sdt_box');
+
-
if($sub_menu.length){
+
-
var left = '170px';
+
-
if($elem.parent().children().length == $elem.index()+1)
+
-
left = '-170px';
+
-
$sub_menu.show().animate({'left':left},200);
+
-
}
+
});
});
}).bind('mouseleave',function(){
}).bind('mouseleave',function(){
-
var $elem = $(this);
+
var $this = $(this);
-
var $sub_menu = $elem.find('.sdt_box');
+
$this.removeClass('selected').children('div').css('z-index','1');
-
if($sub_menu.length)
+
-
$sub_menu.hide().css('left','0px');
+
-
+
-
$elem.find('.sdt_active')
+
-
.stop(true)
+
-
.animate({'height':'0px'},300)
+
-
.andSelf().find('img')
+
-
.stop(true)
+
-
.animate({
+
-
'width':'0px',
+
-
'height':'0px',
+
-
'left':'85px'},400)
+
-
.andSelf()
+
-
.find('.sdt_wrap')
+
-
.stop(true)
+
-
.animate({'top':'25px'},500);
+
});
});
 +
 +
$oe_menu.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items.children('div').hide();
 +
})
 +
            });
 +
</script>
 +
<script type="text/javascript">
 +
     
 +
$(function() {
 +
var $oe_menu2 = $('#oe_menu2');
 +
var $oe_menu_items2 = $oe_menu2.children('li');
 +
 +
                $oe_menu_items2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items2.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
 +
 +
$oe_menu2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items2.children('div').hide();
 +
})
             });
             });
         </script>
         </script>
-
<!-- logo -->
+
<style>
 +
#kaist-footerbox
 +
{
 +
position: relative;
 +
background-color: transparent;
 +
width:1024px;
 +
height:56px;
 +
z-index:2;
 +
margin:0 auto;
 +
bottom:30px;
 +
}
 +
 +
#kaist-footerbox img #footer-img
 +
{
 +
position: center;
 +
width: 1024px;
 +
height:106px;
 +
z-index:2;
 +
opacity:0.9;
 +
margin:0 auto;
 +
}
 +
 +
 +
</style>
 +
</head>
 +
<body>
 +
<div id="main">
-
<header id="header" class="5grid-layout">
 
-
<div class="row">
 
-
<div class="12u" id="logo"> <!-- Logo -->
 
-
 
-
<img height="130" src="images/logo.png" alt=""/>
 
-
 
-
 
-
</div>
 
-
</div>
 
-
<!-- menu bar -->
+
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
 +
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
-
 
+
-
<div class="content">
+
 +
<div class="oe_wrapper">
 +
<ul id="oe_menu" class="oe_menu">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
 +
<div style="top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
 +
</ul>
 +
<ul style="width:100px;margin:0px 5px 0px 5px;">
 +
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
 +
<div style="left:-85px;top:25px;"><!-- -112px -->
 +
<ul>
 +
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
 +
<div style="left:-182px;top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
 +
</ul>
 +
</div>
 +
</li>
-
<ul id="sdt_menu" class="sdt_menu">
+
</ul>
-
<li>
+
</div>
-
<a href="index.html">
+
<div class="oe_wrapper2">
-
<img src="images/2.jpg" alt=""/>
+
-
<span class="sdt_active"></span>
+
-
<span class="sdt_wrap">
+
-
<span class="sdt_link">HOME</span>
+
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
-
</span>
+
-
</a>
+
-
</li>
+
-
<li>
+
-
<a href="team.html">
+
-
<img src="images/1.jpg" alt=""/>
+
-
<span class="sdt_active"></span>
+
-
<span class="sdt_wrap">
+
-
<span class="sdt_link">TEAM</span>
+
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
-
</span>
+
-
</a>
+
-
+
-
</li>
+
-
<li>
+
-
<a href="#">
+
-
<img src="images/3.jpg" alt=""/>
+
-
<span class="sdt_active"></span>
+
-
<span class="sdt_wrap">
+
-
<span class="sdt_link">project</span>
+
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
-
</span>
+
-
</a>
+
-
<div class="sdt_box">
+
-
<a href="#">Overview</a>
+
-
<a href="#">Background</a>
+
-
+
-
</div>
+
-
</li>
+
-
<li>
+
-
<a href="data.html">
+
-
<img src="images/4.jpg" alt=""/>
+
-
<span class="sdt_active"></span>
+
-
<span class="sdt_wrap">
+
-
<span class="sdt_link">LabNote</span>
+
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
-
</span>
+
-
</a>
+
-
<div class="sdt_box">
+
-
<a href="#">Result</a>
+
-
<a href="#">Modeling</a>
+
-
+
-
</div>
+
-
</li>
+
-
<li>
+
-
<a href="#">
+
-
<img src="images/5.jpg" alt=""/>
+
-
<span class="sdt_active"></span>
+
-
<span class="sdt_wrap">
+
-
<span class="sdt_link">Human <br>Practice</span>
+
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
-
</span>
+
-
</a>
+
-
<div class="sdt_box">
+
-
<a href="#">Protocol</a>
+
-
<a href="#">LabNote</a>
+
-
+
-
</div>
+
-
</li>
+
-
<li>
+
<ul id="oe_menu2" class="oe_menu2">
-
<a href="#">
+
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
-
<img src="images/5.jpg" alt=""/>
+
<div style="top:21px;left:32px;">
-
<span class="sdt_active"></span>
+
<ul style="width:100px;height:19px;">
-
<span class="sdt_wrap">
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
-
<span class="sdt_link">Safety</span>
+
</ul>
-
<!--<span class="sdt_descr">Human Practice</span> -->
+
<ul style="width:100px;height:19px;">
-
</span>
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
-
</a>
+
</ul>
-
<div class="sdt_box">
+
<ul style="width:100px;height:19px;">
-
<a href="#">Protocol</a>
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
-
<a href="#">LabNote</a>
+
</ul>
-
+
<ul style="width:100px;height:19px;">
-
</div>
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
-
</li>
+
</ul>
-
+
</div>
-
</ul>
+
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
 +
<div style="left:-91px;top:30px;"><!-- -112px -->
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
 +
</ul>
 +
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
 +
<div style="left:-152px;top:21px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="contact-us">
 +
<div id="contact-us-title">2013 KAIST Korea</div>
 +
<div id="contact-us-information">
 +
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
 +
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
 +
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
</div>
</div>
-
      </header>
+
</div>
 +
 +
<div class="iGEMLogo">
 +
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
 +
</div>
 +
</div>
 +
<div id="main">
 +
<ul class="menu">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea">Home</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Team">Team</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">Project</a>
-
<!-- body -->
+
<!-- <ul>
 +
<li><a href="#" class="documents">Documents</a></li>
 +
<li><a href="#" class="messages">Messages</a></li>
 +
<li><a href="#" class="signout">Sign Out</a></li>
 +
</ul>
 +
 +
-->
-
<div id="page-wrapper">
+
</li>
-
<div class="5grid-layout">
+
<li><a href="#">Labnote</a></li>
-
<div id="feature-content">
+
<li><a href="#">Human Practice</a></li>
-
<section>
+
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Safety">Safety</a></li>
-
<h2>iGEm_KAIST TITLE</h2>
+
-
</section>
+
 +
</ul> <!-- end .menu -->
-
<div class="row">
+
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
-
<div class="12u">
+
-
<div class="row">
+
-
<div class="6u">
+
-
<section id="content">
+
<div id="kaistcontent">
-
<div class="image-style1"><a href="#"><iframe &autoplay=1 width="570" height="315" src="http://www.youtube.com/embed/KHhswxo6xow"frameborder="0" allowfullscreen ></iframe></a></div>
+
-
                            </section>
+
</br>
-
</div>
+
<section id="1st">
-
<div class="3u">
+
<div>
-
<section id = "sidebar">
+
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
-
<div class="image-style1", align="right"><a href="#"><img src="images/pics13.jpg" alt=""></a></div>
+
<h1>Project : Overview</h1>
-
<p>iGEM is International Genetic Engineering M?</p>
+
-
<p class="button-style"><a href="#">GO TO ABSTRACT</a></p>
+
-
</section>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
       
+
-
       
+
-
<div id="page">
+
-
<div class="row">
+
-
<div class="12u">
+
-
<div class="row">
+
-
<div class="8u">
+
-
<section id="content">
+
-
<h2>WhAT IS CRISPR?</h2>
+
-
<p class="subtitle">...</p>
+
-
<p><a href="#"><img src="images/pics02.jpg" alt=""></a></p>
+
-
<p>Welcome to<strong> iGEM_KIAST</strong>, ...
+
-
                                <br><br><br><br><br><br><br><br>
+
-
</section>
+
-
</div>
+
-
<div class="4u">
+
-
<section id="sidebar">
+
-
<h2>OUR TEAM PROJECT...</h2>
+
-
<p class="subtitle">genome editing using CRISPR</p>
+
-
<p>Pathway............
+
-
                                <br><br></p>
+
-
+
-
</section>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
-
<!--<div id="footer-wrapper">
+
-
<div id="footer-content" class="5grid-layout">
+
-
<div class="row">
+
-
<div class="3u">
+
</br>
-
+
</div>
</div>
-
<div class="6u">
+
-
<section>
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
-
<h2>footer site</h2>
+
</br>
-
<p>iGEM logo </p>
+
<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
-
+
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The <span id="starter">RAPTOR</span> (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
-
</section>
+
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
-
</div>
+
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
-
</div>
+
</br></br></span>
-
</div>
+
<div clear='both'></div>
-
</div> -->
+
-
<div class="5grid-layout">
+
-
<div id="copyright">
+
-
<section>
+
-
<p>&copy; Your Site Name | Images: <a href="http://fotogrph.com/">Fotogrph</a> | Design: <a href="http://html5templates.com/">HTML5Templates.com</a></p>
+
-
</section>
+
</br></br>
 +
<div align='right'><a href="#top">▲Back to the top</a></div></br>
 +
 +
 +
</section>
 +
 +
</div>
</div>
 +
</div>
 +
<div id="kaist-footerbox"><img id="footer-img" align="middle" src="https://static.igem.org/mediawiki/2013/e/ef/Footer_background_kaist.png"></img>
</div>
</div>
</body>
</body>
</html>
</html>

Latest revision as of 10:12, 23 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.