Team:BIT

From 2013.igem.org

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  <table width="1024" border="0" class="bg">
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      <!-- HEADER -->
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        <tr>
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      <div id="heads">
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          <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT&action=edit"></a></div></td>
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        <div class="fleft"><a href="http://www.bit.edu.cn"><img src="https://static.igem.org/mediawiki/2013/6/63/Bitlogo.gif" alt="" /></a></div>
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          <td width="167"><div><a id="a2" href="https://2013.igem.org/Main_Page"></a></div></td>
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          <td width="130"><div><a id="a3" href="https://2013.igem.org/wiki/index.php?title=Special:UserLogout&returnto=Main_Page"></a></div></td>
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<ul>
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                  <div class="left-top-corner">
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<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/Team">TEAM</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/Project">PROJECT</a></li>
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                          <h3>HEXAVALENT CHROMIUM</h3>
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<li><a href="https://2013.igem.org/Team:BIT/Parts">PARTS</a></li>
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                        </div>
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<li><a href="https://2013.igem.org/Team:BIT/Modeling">MODELING</a></li>
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                    </div>
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    <li><a href="https://2013.igem.org/Team:BIT/HP">HUMAN PRACTICE</a></li>
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                  </div>
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    <li><a href="https://2013.igem.org/Team:BIT/Safety">SAFETY</a></li>
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                  <div class="xcontent">
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    <li><a href="https://2013.igem.org/Team:BIT/Notes">NOTES</a></li>
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                                      <p>Some illegal dairies always add leather hydrolysate into fresh milk and powdered milk to increase the percentage of protein in milk. Chromate, which is one of the elements of leather dye, is the main element that can be used to trace leather hydrolysate. Our Cr(Ⅵ)-biosensor is thus designed for the detection of chromate in dairy products.</p>
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                                      <div class="link1"><a href="#"><em><b>more</b></em></a></div>
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                        <div class="border-top">
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                          <h3>TETRACYCLINE</h3>
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                        </div>
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                                      <p>Our detection of tetracycline, one of the antibiotics that are widely used in modern dairy industry to prevent the infection of cow breasts, was realised by two inhibitor proteins, LacI and TetR. When certain chemicals, such as tetracycline and IPTG, are added, they will prevent the inhibitor from binding to their respective promoters. Thus, the promoters will be unlocked and the green fluorescence protein will be produced.</p>
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                                      <div class="link1"><a href="#"><em><b>more</b></em></a></div>
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                          <h3>β-LACTAM</h3>
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                        </div>
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                                    <div class="inner">
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                                      <p>β-LACTAM</p>
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                                      <div class="link1"><a href="#"><em><b>more</b></em></a></div>
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                          <h3>SIGNAL</h3>
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                                      <p>Besides the three bio-sensors, we also introduced an amplifier and a controller so that the output signal could be amplified by adjusting the concentration of IPTG to achieve the adjustment of magnification. In order to achieve real-time detection, we also made a biochip, which provided a reaction room for both engineering bacteria and samples, in a hand-held electronic equipment made by ourselves.</p>
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                                <p align="center">A Brief Introduction of Integrated Sensor for  Detection of Milk Product </p>
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                                <p align="left">&nbsp;</p>
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                                <p align="left">As we know, antibiotics are widely used in modern  industry to prevent the infection of cow breasts. However, the residual of antibiotics  and other components, such as Cr (VI) which is widely used in the recycle of  proteins from leather, will endanger the health of customers. Therefore, it is necessary  to find solution to identify these harmful chemicals, but the method of timely  detection of harmful chemicals in products still remains unsolved. <br />
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                                  To solve this problem,  our sensor  has been divided  into three,  respectively, of hexavalent  chromium, tetracycline  and β-lactam  there is a  response, for each sensor,  we are all  assembled  in  a downstream of  the  amplification block (amplifier) ​​and the control block (controller),  the output  signal to  be  amplified,  by adjusting  the  concentration of IPTG to achieve the adjustment of magnification.  Amplifier  by the  T7 RNA  polymerase gene  and T7  promoter  composition  control  block  is through the  downstream of  the T7 promoter  plus  lacO and the  addition of regular  expression  lacI, by  adjusting the  concentration of IPTG, lacO then adjust the boot situation achieved,  the final  response module for the green  fluorescent  protein.<br />
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                                  In order to achieve real-time detection, we  also made a biochip which provided a reaction room for both engineering  bacteria and samples. Now we are able to detect all these three harmful  chemicals with a hand-held electronic equipment made by ourselves.</p>
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      <td class="t1">A Brief Introduction of Integrated Sensor for Detection of Milk Product</td>
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      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. <br/>
 +
  &nbsp;&nbsp;&nbsp;&nbsp;To solve this problem, our sensor has been divided into two, respectively, of <a id="b1" href="https://2013.igem.org/Team:BIT/project_biosensors">tetracycline and β-lactam</a>. There is a response, for each sensor. They are all assembled in a downstream of the <a id="b1" href="https://2013.igem.org/Team:BIT/project_AC">amplification block</a> (amplifier) and the <a  id="b1" href="https://2013.igem.org/Team:BIT/project_AC">control block </a>(controller). The output signal will be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus <i>lacO</i> and the addition of regular expression <i>lacI</i>, by adjusting the concentration of IPTG, <i>lacO</i> then adjust the boot situation achieved, the final response module for the green fluorescent protein.<br/>
 +
  &nbsp;&nbsp;&nbsp;&nbsp;In order to achieve real-time detection, we also made a <a  id="b1" href="https://2013.igem.org/Team:BIT/project_microchip">biochip</a> which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a<a id="b1" href="https://2013.igem.org/Team:BIT/project_hardware"> hand-held electronic equipment</a> made by ourselves.</td>
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        <td width="790">Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081 </td>
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Latest revision as of 13:38, 28 October 2013

iGEM BIT

     
  Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081  
  E-mail: yifei0114@bit.edu.cn  
  Beijing Institute of Technology © 2013 Privacy Policy  

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