Team:Paris Bettencourt/Notebook/Drug Screening/Friday 19th July.html

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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Drug_Screen" target="_blank" class="tbnotelogo DSlogo"> ASDF </a>
<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Drug_Screen" target="_blank" class="tbnotelogo DSlogo"> ASDF </a>
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<h3>Day Number<sup>suffix</sup> Month</h3>
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<h3>Friday 19<sup>th</sup> July</h3>
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<ul>
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<li><font color="#EE0F29">sD012/sD013</font>=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes
<li><font color="#EE0F29">sD012/sD013</font>=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes
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<li><FONT COLOR="#333333">sD016/sD017</FONT>=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin
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<li><FONT COLOR="#EE0F29">sD016/sD017</FONT>=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin
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<li><FONT COLOR="#222222">sD014/sD015</FONT>=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol
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<li><FONT COLOR="#EE0F29">sD014/sD015</FONT>=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol
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<br><b>Glycerol Stocks were made of the above mentioned strains</b>
<br><b>Glycerol Stocks were made of the above mentioned strains</b>

Latest revision as of 10:31, 13 August 2013

Drug Screening

Friday 19th July

TRANSFORMATIONS WORKED!!! cells were stocked and entered in catalog.

  • sD012/sD013=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes
  • sD016/sD017=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin
  • sD014/sD015=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol

Glycerol Stocks were made of the above mentioned strains
2 Single colonies from each agar plate of above mentioned strains were picked and used to inoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001+pD004, sD001+sD004+sD005, sD001+sD004+sD006;
one set was frozen at -20ºC and the other set was frozen at -80ºC.