12/08/13

From 2013.igem.org

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(Running an agarose gel for sonication analysis)
 
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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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==Sonicating herring sperm DNA==
==Sonicating herring sperm DNA==
*Pipetting out sonicated samples every 30mins
*Pipetting out sonicated samples every 30mins
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*Add buffer so that wells in the tray and the tray itself are covered
*Add buffer so that wells in the tray and the tray itself are covered
*Run at 80 V for an hour and a half (gel is 1.2%)
*Run at 80 V for an hour and a half (gel is 1.2%)
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[[File:herringsperm_sonicate_130813.jpg]]

Latest revision as of 14:18, 13 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Sonicating herring sperm DNA

  • Pipetting out sonicated samples every 30mins
    • Pipette 4ul of DNA into Son 1-6 every 30mins
      • Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
      • Vortex each dilution thoroughly and centrifuge briefly.
  • Keep tubes on ice

Running an agarose gel for sonication analysis

  • To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
  • Use 5 ul of 1kb marker on both ends
  • Add buffer so that wells in the tray and the tray itself are covered
  • Run at 80 V for an hour and a half (gel is 1.2%)

Herringsperm sonicate 130813.jpg