12/08/13
From 2013.igem.org
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TanviSinha (Talk | contribs) (→Running an agarose gel for sonication analysis) |
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+ | <div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;"> | ||
+ | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
+ | !align="center"|[[Team:Leicester|Home]] | ||
+ | !align="center"|[[Team:Leicester/Team|Team]] | ||
+ | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
+ | !align="center"|[[Team:Leicester/Project|Project]] | ||
+ | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
+ | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
+ | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
+ | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
+ | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
+ | |} | ||
+ | </div> | ||
+ | |||
==Sonicating herring sperm DNA== | ==Sonicating herring sperm DNA== | ||
*Pipetting out sonicated samples every 30mins | *Pipetting out sonicated samples every 30mins | ||
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*Add buffer so that wells in the tray and the tray itself are covered | *Add buffer so that wells in the tray and the tray itself are covered | ||
*Run at 80 V for an hour and a half (gel is 1.2%) | *Run at 80 V for an hour and a half (gel is 1.2%) | ||
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+ | [[File:herringsperm_sonicate_130813.jpg]] |
Latest revision as of 14:18, 13 August 2013
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Sonicating herring sperm DNA
- Pipetting out sonicated samples every 30mins
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
- Vortex each dilution thoroughly and centrifuge briefly.
- Pipette 4ul of DNA into Son 1-6 every 30mins
- Keep tubes on ice
Running an agarose gel for sonication analysis
- To tubes 1-6, add 14 ul of sterile water and 5 ul of orange G dye and 1ul of DNA from Dil x10 1-6 appropriately. Centrifuge briefly. Keep on ice until loading.
- Use 5 ul of 1kb marker on both ends
- Add buffer so that wells in the tray and the tray itself are covered
- Run at 80 V for an hour and a half (gel is 1.2%)