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15/07/13
From 2013.igem.org
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{|border=1 | {|border=1 | ||
| + | |-bgcolor=grey | ||
|Plate||Colonies | |Plate||Colonies | ||
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|- | |- | ||
|20ul of 50pg/ul||135 | |20ul of 50pg/ul||135 | ||
| + | |- | ||
| + | |20ul of 10pg/ul||96 | ||
| + | |- | ||
| + | |200ul of 50pg/ul||~1600 | ||
| + | |- | ||
| + | |200ul of 10pg/ul||944 | ||
| + | |} | ||
| + | |||
| + | Therefore there was 1.2x10^8 cfu/ug | ||
| + | |||
| + | |||
| + | ==Replate to get individual colonies== | ||
| + | |||
| + | *Take an individual colonies | ||
| + | *Streak out onto new plate | ||
| + | *Sterilize loop using bunsen burner | ||
| + | *Incubate at 37 overnight | ||
Latest revision as of 10:30, 14 August 2013
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Count colonies on agar plates
| Plate | Colonies |
| Viability control | Covered |
| Negative control | 1 contaminant |
| 20ul of 50pg/ul | 135 |
| 20ul of 10pg/ul | 96 |
| 200ul of 50pg/ul | ~1600 |
| 200ul of 10pg/ul | 944 |
Therefore there was 1.2x10^8 cfu/ug
Replate to get individual colonies
- Take an individual colonies
- Streak out onto new plate
- Sterilize loop using bunsen burner
- Incubate at 37 overnight
