Exeter/8 August 2013

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Latest revision as of 20:38, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Liquid Cultures, Miniprepped

[http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM

[http://parts.igem.org/Part:BBa_J04450 RFP control] CAM

[http://parts.igem.org/Part:BBa_R0082 R0082] (ompC promoter) CAM

DH5α 3 (Tom and Adam's control) TET

DH5α 5 (Tom and Adam's RBS + Cyan) TET

DH5α 1 (Tom and Adam's RBS + cph8) TET

[http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM

[http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM

[http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + cph8) CAM

[http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (RBS + promoter) CAM

[http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM

[http://parts.igem.org/Part:BBa_K322124 K322124] (RBS + cph8) CAM

( 3 replicates, #2, #3 and #4.)

NanoDropping Promoter + RBS Replicates

1 = 29.6ng/ul

1 = 29.2ng/ul

2 = 12.2ng/ul

2 = 12.5ng/ul

3 = 22.8ng/ul

3 = 23.6ng/ul

4 = 17.8ng/ul

4 = 17.5ng/ul

NanoDrop of liquid cultures

Liquid culture	NanoDrop result (ng/ul)
Magenta	106.9
RFP	42.9
ompC	117.3
DH5α 3	46.3
S05058	67.1
Yellow	57.1
RBS + cph8	113.7
cph8 #4	49.2 and 48.8
cph8 #2	221.9 and 180.7
cph8 #3	70.8 and 70.8
Promoter and RBS	99.9
B0015	52.1
AMP plasmid	82.5

We sent off cph8 K322124 red light sensor for sequencing.

2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli

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+{{:Team:Exeter/Template/Header}}
+<div class="container">
+    <div class="row">
+        <div class="span" style="text-align:justify">
 == Liquid Cultures, Miniprepped ==
-* K592012 (Magenta) CAM
+* [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
-* RFP control CAM
+* [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
-* R0082 (OmpF promoter) CAM
+* [http://parts.igem.org/Part:BBa_R0082 R0082] (''ompC'' promoter) CAM
 * DH5α 3 (Tom and Adam's control) TET
@@ Line 14: / Line 19: @@
 * DH5α 1 (Tom and Adam's RBS + cph8) TET
-* S05058 (RBS + yellow) CAM
+* [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
-* K592010 (yellow) CAM
+* [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
-* K592018 (RBS + cph8) CAM
+* [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + cph8) CAM
-* K608002 (RBS + promoter) CAM
+* [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (RBS + promoter) CAM
-* B0015 (Terminator) CAM
+* [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
-* K322124 (RBS + cph8) CAM
+* [http://parts.igem.org/Part:BBa_K322124 K322124] (RBS + cph8) CAM
 ( 3 replicates, #2, #3 and #4.)
 ==NanoDropping Promoter + RBS Replicates==
@@ Line 59: / Line 63: @@
 | RFP || 42.9
 |-
-| OmpF || 117.3
+| ''ompC'' || 117.3
 |-
 | DH5α 3 || 46.3
@@ Line 87: / Line 91: @@
 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.
+Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
+   </div>
+</div>
+{{:Team:Exeter/Template/Footer}}