Team:Groningen/Labwork/15 August 2013

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<br>New plates are made with agar concentration of 0.4%, 0.2% and 0.1% to see if the concentration of agar could be the problem.
<br>New plates are made with agar concentration of 0.4%, 0.2% and 0.1% to see if the concentration of agar could be the problem.
<br>Plates are to dry overnight.
<br>Plates are to dry overnight.
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<h2>Sebas</h2>
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Did a colony PCR op deltaDES/CheY tet with primers HM07&HM10 (Fw tet with Rev downstream des) colony 2 had the right <Br>insert. Inoculated 3ml LB with colonie 2.
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<Br>Did a miniprep on the other backbone with GFP and digested it with XbaI and PstI. Digested the hyperspank <br>backbone with XbaI and PstI and did a ligation O/N in a beaker with RT water in the fridge.
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<br>There where ~10 colonies on the negative control, ~30 on the 100µl plate and ~300 on the extra concentrated. <Br>plate. Picked 3 colonies and grew them O/N 
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Latest revision as of 11:57, 20 August 2013

Inne

Plates that were made yesterday showed no difference betweeen the supposed motile and no motile bacteria.
New plates are made with agar concentration of 0.4%, 0.2% and 0.1% to see if the concentration of agar could be the problem.
Plates are to dry overnight.

Sebas

Did a colony PCR op deltaDES/CheY tet with primers HM07&HM10 (Fw tet with Rev downstream des) colony 2 had the right
insert. Inoculated 3ml LB with colonie 2.

Did a miniprep on the other backbone with GFP and digested it with XbaI and PstI. Digested the hyperspank
backbone with XbaI and PstI and did a ligation O/N in a beaker with RT water in the fridge.

There where ~10 colonies on the negative control, ~30 on the 100µl plate and ~300 on the extra concentrated.
plate. Picked 3 colonies and grew them O/N