Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
 
(76 intermediate revisions not shown)
Line 1: Line 1:
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
-
<head>
+
<head>
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<meta name="generator" content="MediaWiki 1.16.0" />
+
<meta name="generator" content="MediaWiki 1.16.5" />
-
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=edit" />
+
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=edit" />
+
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
<link rel="shortcut icon" href="/favicon.ico" />
<link rel="shortcut icon" href="/favicon.ico" />
-
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2012.igem.org (en)" />
+
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:KAIST Korea/Project Background - 2012.igem.org</title>
+
<meta name="viewport" content="width=device-width, initial-scale=1.0 minimum-scale=1, maximum-scale=1, user-scalable=yes, target-densitydpi=medium-dpi" />
-
<style type="text/css" media="screen, projection">/*<![CDATA[*/
+
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic' rel='stylesheet' type='text/css'>
 +
<title>Team:KAIST Korea/Project Background - 2013.igem.org</title>
 +
<style type="text/css" media="screen, projection">/*<![CDATA[*/
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/igem/main.css?270";
@import "/wiki/skins/igem/main.css?270";
-
/*]]>*/</style>
+
#bar{z-index : 5;
-
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
+
}
-
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
+
#menu,
-
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
+
#menu ul,
-
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
+
#menu li,
-
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
+
#menu a {
-
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
+
margin: 0;
-
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
+
padding-left: 40;
-
+
border: none;
-
<script>
+
outline: none;
-
var skin="igem",
+
}
-
stylepath="/wiki/skins",
+
.menu {
-
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
+
height: 50px;
-
wgArticlePath="/$1",
+
weight: 100%;
-
wgScriptPath="/wiki",
+
padding-left: 30%;
-
wgScriptExtension=".php",
+
         z-index: 2;
-
wgScript="/wiki/index.php",
+
background: #000000;
-
wgVariantArticlePath=false,
+
}
-
wgActionPaths={},
+
-
wgServer="https://2013.igem.org",
+
-
wgCanonicalNamespace="",
+
-
wgCanonicalSpecialPageName=false,
+
-
wgNamespaceNumber=0,
+
-
wgPageName="Team:KAIST_Korea/Project_Background",
+
-
wgTitle="Team:KAIST Korea/Project Background",
+
-
wgAction="view",
+
-
wgArticleId=9665,
+
-
wgIsArticle=true,
+
-
wgUserName="Springkjw",
+
-
wgUserGroups=["*", "user", "autoconfirmed"],
+
-
wgUserLanguage="en",
+
-
wgContentLanguage="en",
+
-
wgBreakFrames=false,
+
-
wgCurRevisionId=297918,
+
-
wgVersion="1.16.0",
+
-
wgEnableAPI=true,
+
-
wgEnableWriteAPI=true,
+
-
wgSeparatorTransformTable=["", ""],
+
-
wgDigitTransformTable=["", ""],
+
-
wgMainPageTitle="Main Page",
+
-
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2012.igem.org", "5": "2012.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
+
-
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2012.igem.org": 4, "2012.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
+
-
wgSiteName="2012.igem.org",
+
-
wgCategories=[],
+
-
wgMWSuggestTemplate="https://2012.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
+
-
wgDBname="2012_igem_org",
+
-
wgSearchNamespaces=[0],
+
-
wgMWSuggestMessages=["with suggestions", "no suggestions"],
+
-
wgRestrictionEdit=[],
+
-
wgRestrictionMove=[],
+
-
wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"};
+
-
</script>               
+
-
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
+
-
<!-- Head Scripts -->
+
-
<script src="/wiki/skins/common/ajax.js?270"></script>
+
-
<script src="/wiki/skins/common/ajaxwatch.js?270"></script>
+
-
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
+
-
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;smaxage=0&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
+
-
<!-- jQuery Javascript -->
+
-
<script type='text/javascript'         src ='/common/jquery-latest.min.js'></script>
+
-
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
+
-
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
+
-
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
+
-
</head>
+
   
   
-
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_KAIST_Korea_Project_Background">
+
.menu li {
-
  <div id="globalWrapper">
+
position: relative;
 +
list-style: none;
 +
margin: 0px auto 0 auto;
 +
float: left;
 +
display: block;
 +
height: 50px;
 +
}
   
   
-
    <div id="top-section">
+
/* Links */
-
<div id="p-logo">
+
-
    <a href="/Main_Page"
+
-
      title="Main Page">
+
-
    <img src=''>
+
-
    </a>
+
-
</div>  <!-- end p-logo -->
+
-
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
+
   
   
 +
.menu li a {
 +
margin:0px auto;
 +
display: block;
 +
padding: 0 14px;
 +
margin: 6px 0;
 +
 +
text-decoration: none;
 +
 +
   
   
 +
font-family: Helvetica, Arial, sans-serif;
 +
font-weight: bold;
 +
font-size: 20px;
   
   
-
<div id="menubar" class='left-menu noprint'>
+
color: #353535;
-
  <ul>
+
-
                  <li
+
-
                class='selected'       ><a href="/Team:KAIST_Korea/Project_Background">Page              </a></li>
+
-
              <li
+
-
                class='new'       ><a href="/wiki/index.php?title=Talk:Team:KAIST_Korea/Project_Background&amp;action=edit&amp;redlink=1">Discussion              </a></li>
+
-
              <li
+
-
                    ><a href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=edit">Edit              </a></li>
+
-
              <li
+
-
                    ><a href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=history">History              </a></li>
+
-
              <li
+
-
                    ><a href="/Special:MovePage/Team:KAIST_Korea/Project_Background">Move              </a></li>
+
-
              <li
+
-
                    ><a href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=watch">Watch              </a></li>
+
-
              <li style='color:#808080;cursor:default'>teams</li>
+
-
  </ul>
+
-
</div> <!-- end menubar (left) -->
+
   
   
-
<div class="right-menu noprint" id="menubar">
+
-webkit-transition: color .2s ease-in-out;
-
    <ul>
+
-moz-transition: color .2s ease-in-out;
-
                <li id="pt-userpage"><a href="/User:Springkjw" title="Your user page [.]" accesskey="." class="new">Springkjw</a></li>                              <li id='pt-preferences'><a href='https://igem.org/User_Information' title='My account'>My account</a></li>
+
-o-transition: color .2s ease-in-out;
-
                          <li id="pt-logout"><a href="/wiki/index.php?title=Special:UserLogout&amp;returnto=Team:KAIST_Korea/Project_Background" title="Log out">Log out</a></li>     </ul>
+
-ms-transition: color .2s ease-in-out;
-
</div><!-- end right menubar -->
+
transition: color .2s ease-in-out;
 +
}
   
   
-
<div id="search-controls" class="noprint">
+
.menu li:first-child a { border-left: none; }
-
<form action="/Special:Search" id="searchform">
+
.menu li:last-child a{ border-right: none; }
-
<input id="searchInput" name="search" type="text" title="Search 2012.igem.org [f]" accesskey="f" value="" />
+
-
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
+
.menu li:hover > a { color: #F29661; }
-
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
+
-
</form>
+
/* Sub Menu */
-
</div> <!-- close search controls -->
+
-
    </div> <!-- close top-section-->
+
.menu ul {
-
    <div id="content">
+
position: absolute;
-
<a name="top" id="top"></a>
+
top: 50px;
-
<h1 class="firstHeading">Team:KAIST Korea/Project Background</h1>
+
-
<div id="bodyContent">
+
left: 0px;
-
<h3 id="siteSub" class='noprint'>From 2012.igem.org</h3>
+
-
<div id="contentSub"></div>
+
padding-right: 10px;
-
<!--
+
-
<div id="jump-to-nav">Jump to:                       <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
+
opacity: 0;
-
<!-- start content -->
+
-
<p>
+
background: #1f2024;
-
    <head>
+
-
<title>KAIST Korea 2012 iGEM</title>
+
-webkit-border-radius: 0 0 5px 5px;
-
<style type="text/css">
+
-moz-border-radius: 0 0 5px 5px;
 +
border-radius: 0 0 5px 5px;
 +
 +
-webkit-transition: opacity .25s ease .1s;
 +
-moz-transition: opacity .25s ease .1s;
 +
-o-transition: opacity .25s ease .1s;
 +
-ms-transition: opacity .25s ease .1s;
 +
transition: opacity .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul { opacity: 1; }
 +
 +
.menu ul li {
 +
height: 0;
 +
overflow: hidden;
 +
padding: 0px;
 +
 +
-webkit-transition: height .25s ease .1s;
 +
-moz-transition: height .25s ease .1s;
 +
-o-transition: height .25s ease .1s;
 +
-ms-transition: height .25s ease .1s;
 +
transition: height .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul li {
 +
height: 40px;
 +
overflow: visible;
 +
padding: 0;
 +
}
 +
 +
.menu ul li a {
 +
width: 100px;
 +
padding: 4px 0 4px 40px;
 +
margin: 0;
 +
 +
border: none;
 +
border-bottom: 1px solid #353539;
 +
}
 +
 +
.menu ul li:last-child a { border: none; }
 +
 +
/* Icons */
 +
 +
.menu a.documents { background: url(docs.png) no-repeat 6px center; }
 +
.menu a.messages { background: url(bubble.png) no-repeat 6px center; }
 +
.menu a.signout { background: url(arrow.png) no-repeat 6px center; }
 +
 +
 +
ul.menu { margin: 0px auto 0 auto; }
 +
</style>
 +
 
 +
<style type="text/css">
/* Cross-Browsing */
/* Cross-Browsing */
Line 163: Line 165:
margin: 0;
margin: 0;
padding: 0;
padding: 0;
-
}
+
}  
 +
 
   
   
Line 185: Line 188:
clear: both;
clear: both;
width: 100%;
width: 100%;
-
        z-index: 1;
+
                z-index: 1;
position: relative;
position: relative;
top:0px;
top:0px;
Line 251: Line 254:
font-size: 0pt;
font-size: 0pt;
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
-
        z-index: 100;
+
                z-index: 100;
background-position: left 0px;
background-position: left 0px;
cursor: pointer;
cursor: pointer;
Line 284: Line 287:
}
}
-
#top-section
+
-
{
+
-
height: 35px;
+
-
width: 1024px;
+
-
margin: 0 auto;
+
-
top : 0px;
+
-
background-image: url("https://static.igem.org/mediawiki/2012/5/5a/2012Kaist_korea.png");
+
-
background-repeat: no-repeat;
+
-
background-color: #3d3d3d;
+
-
background-position: center;
+
-
border-bottom: 1px solid #262B35;
+
-
border-left: medium none;
+
-
border-right: medium none;
+
-
        z-index: 100;
+
-
}
+
#menubar
#menubar
Line 352: Line 341:
#footer li a:active{ color: #ababab; text-decoration: none; font-weight: bold}
#footer li a:active{ color: #ababab; text-decoration: none; font-weight: bold}
         </style>
         </style>
-
    </head>
 
-
 
-
 
-
<head>
 
-
<meta name="viewport" content="width=device-width, initial-scale=1.0 minimum-scale=1, maximum-scale=1, user-scalable=yes, target-densitydpi=medium-dpi" />
 
-
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic' rel='stylesheet' type='text/css'>
 
<style>  
<style>  
@import url(http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic);
@import url(http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic);
Line 536: Line 519:
position: relative;
position: relative;
top: -5px;
top: -5px;
-
    left:292px;
+
        left:294px;
-
width:732px;
+
width:729px;
height: 250px;
height: 250px;
z-index: 0;
z-index: 0;
Line 603: Line 586:
position: absolute;
position: absolute;
z-index:2;
z-index:2;
-
top:425px;
+
top:450px;
padding:4px 0px 0px 10px;
padding:4px 0px 0px 10px;
width:268px;
width:268px;
Line 656: Line 639:
     s.parentNode.insertBefore(wf, s);
     s.parentNode.insertBefore(wf, s);
   })(); </script>
   })(); </script>
-
</head>
+
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
-
+
<meta http-equiv="imagetoolbar" content="no" />
-
<body>
+
<script>  
-
<div id="main">
+
var skin="igem",
-
<a href="https://2012.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2012/5/59/Logopart_Kaist.png"/></a>
+
stylepath="/wiki/skins",
-
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
+
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
-
<div class="oe_wrapper">
+
wgArticlePath="/$1",
-
<ul id="oe_menu" class="oe_menu">
+
wgScriptPath="/wiki",
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
+
wgScriptExtension=".php",
-
<div style="top:30px;">
+
wgScript="/wiki/index.php",
-
<ul style="margin:0 0 0 30px;width:100px;">
+
wgVariantArticlePath=false,
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
+
wgActionPaths={},
-
</ul>
+
wgServer="https://2013.igem.org",
-
<ul style="width:100px;margin:0px 5px 0px 5px;">
+
wgCanonicalNamespace="",
-
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
+
wgCanonicalSpecialPageName=false,
-
</ul>
+
wgNamespaceNumber=0,
-
</div>
+
wgPageName="Team:KAIST_Korea/Project",
-
</li>
+
wgTitle="Team:KAIST Korea/Project",
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
+
wgAction="view",
-
<div style="left:-85px;top:25px;"><!-- -112px -->
+
wgArticleId=9665,
-
<ul>
+
wgIsArticle=true,
-
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
+
wgUserName="Springkjw",
-
</ul>
+
wgUserGroups=["*", "user", "autoconfirmed"],
-
<ul style="padding:5px 0px 5px 0px">
+
wgUserLanguage="en",
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
+
wgContentLanguage="en",
-
</ul>
+
wgBreakFrames=false,
-
<ul style="padding:5px 0px 5px 0px">
+
wgCurRevisionId=297918,
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
+
wgVersion="1.16.0",
-
</ul>
+
wgEnableAPI=true,
-
</div>
+
wgEnableWriteAPI=true,
-
</li>
+
wgSeparatorTransformTable=["", ""],
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
+
wgDigitTransformTable=["", ""],
-
<div style="left:-182px;top:30px;">
+
wgMainPageTitle="Main Page",
-
<ul style="margin:0 0 0 30px;width:100px;">
+
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
+
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
-
</ul>
+
wgSiteName="2013.igem.org",
-
<ul style="width:100px">
+
wgCategories=[],
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
+
wgMWSuggestTemplate="https://2013.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
-
</ul>
+
wgDBname="2013_igem_org",
-
</div>
+
wgSearchNamespaces=[0],
-
</li>
+
wgMWSuggestMessages=["with suggestions", "no suggestions"],
-
+
wgRestrictionEdit=[],
-
</ul>
+
wgRestrictionMove=[],
-
</div>
+
wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"};
-
<div class="oe_wrapper2">
+
</script>              
-
<ul id="oe_menu2" class="oe_menu2">
+
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">PROJECT</a>
+
<!-- Head Scripts -->
-
<div style="top:21px;left:32px;">
+
<script src="/wiki/skins/common/ajax.js?270"></script>
-
<ul style="width:100px;height:19px;">
+
<script src="/wiki/skins/common/ajaxwatch.js?270"></script>
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
+
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
-
</ul>
+
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;smaxage=0&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
-
<ul style="width:100px;height:19px;">
+
<!-- jQuery Javascript -->
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
+
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
-
</ul>
+
<script type='text/javascript'         src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
-
<ul style="width:100px;height:19px;">
+
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
+
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
-
</ul>
+
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
-
<ul style="width:100px;height:19px;">
+
<script type='text/javascript'         src ='/forum/forum_scripts.js'></script>
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
+
 
-
</ul>
+
 
-
</div>
+
-
</li>
+
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
+
-
<div style="left:-91px;top:30px;"><!-- -112px -->
+
-
<ul style="margin:0 0 0 30px;width:100px;">
+
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
+
-
</ul>
+
-
<ul style="width:100px">
+
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
+
-
</ul>
+
-
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
+
-
<div style="left:-152px;top:21px;">
+
-
<ul style="width:100px;height:19px;">
+
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
+
-
</ul>
+
-
<ul style="width:100px;height:19px;">
+
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
+
-
</ul>
+
-
<ul style="width:100px;height:19px;">
+
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
+
-
</ul>
+
-
<ul style="width:100px;height:19px;">
+
-
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
+
-
</ul>
+
-
</div>
+
-
</li>
+
-
+
-
</ul>
+
-
</div>
+
-
<div class="contact-us">
+
-
<div id="contact-us-title">2012 KAIST Korea</div>
+
-
<div id="contact-us-information">
+
-
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
+
-
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
+
-
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
+
-
</div>
+
-
</div>
+
-
+
-
<div class="iGEMLogo">
+
-
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
+
-
</div>
+
-
</div>
+
-
        <!-- The JavaScript -->
+
-
        <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
+
-
        <script type="text/javascript">
+
-
            $(function() {
+
-
var $oe_menu = $('#oe_menu');
+
-
var $oe_menu_items = $oe_menu.children('li');
+
-
+
-
                $oe_menu_items.bind('mouseenter',function(){
+
-
var $this = $(this);
+
-
$this.addClass('slided selected');
+
-
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
+
-
$oe_menu_items.not('.slided').children('div').hide();
+
-
$this.removeClass('slided');
+
-
});
+
-
}).bind('mouseleave',function(){
+
-
var $this = $(this);
+
-
$this.removeClass('selected').children('div').css('z-index','1');
+
-
});
+
-
+
-
$oe_menu.bind('mouseenter',function(){
+
-
var $this = $(this);
+
-
$this.addClass('hovered');
+
-
}).bind('mouseleave',function(){
+
-
var $this = $(this);
+
-
$this.removeClass('hovered');
+
-
$oe_menu_items.children('div').hide();
+
-
})
+
-
            });
+
-
</script>
+
-
<script type="text/javascript">
+
-
     
+
-
$(function() {
+
-
var $oe_menu2 = $('#oe_menu2');
+
-
var $oe_menu_items2 = $oe_menu2.children('li');
+
-
+
-
                $oe_menu_items2.bind('mouseenter',function(){
+
-
var $this = $(this);
+
-
$this.addClass('slided selected');
+
-
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
+
-
$oe_menu_items2.not('.slided').children('div').hide();
+
-
$this.removeClass('slided');
+
-
});
+
-
}).bind('mouseleave',function(){
+
-
var $this = $(this);
+
-
$this.removeClass('selected').children('div').css('z-index','1');
+
-
});
+
-
+
-
$oe_menu2.bind('mouseenter',function(){
+
-
var $this = $(this);
+
-
$this.addClass('hovered');
+
-
}).bind('mouseleave',function(){
+
-
var $this = $(this);
+
-
$this.removeClass('hovered');
+
-
$oe_menu_items2.children('div').hide();
+
-
})
+
-
            });
+
-
        </script>
+
-
</body>
+
-
+
-
</p><p>
+
-
<head>
+
<link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
<script type="text/javascript">
<script type="text/javascript">
Line 945: Line 822:
   
   
</style>
</style>
-
</head>
+
  <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
-
   
+
        <script type="text/javascript">
-
+
            $(function() {
-
<body>
+
var $oe_menu = $('#oe_menu');
-
<div id="main">
+
var $oe_menu_items = $oe_menu.children('li');
-
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
+
-
+
-
+
-
<div id="menucontent">
+
-
<img src="https://static.igem.org/mediawiki/2012/e/e8/Project_ico.png"/></a>
+
-
<a href="https://2012.igem.org/Team:KAIST_Korea/Project_Background"><img src="https://static.igem.org/mediawiki/2012/e/e3/Overview_ico.png"/></a>
+
-
<a href="https://2012.igem.org/Team:KAIST_Korea/Project_Results"><img src="https://static.igem.org/mediawiki/2012/5/5e/Results_ico.png"/></a>
+
-
<a href="https://2012.igem.org/Team:KAIST_Korea/Project_Future"><img src="https://static.igem.org/mediawiki/2012/9/95/Future_ico.png"/></a>
+
-
<a href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling"><img src="https://static.igem.org/mediawiki/2012/4/4c/Model_ico.png"/></a>
+
-
</div>
+
-
+
-
<div id="top-img-description-box"><span id="top-img-description">Project : Overview</span></div>
+
-
<img id="horizontal-line" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img>
+
-
+
-
<div id="kaistcontent">
+
-
+
-
<div align='center'>
+
-
<a href="#1st"><span id="tab"> <b>Overview</b> </span></a>
+
-
<a href="#2nd"><span id="tab"><b>Filp Flop</b></span></a>
+
-
<a href="#3rd"><span id="tab"><b>Reguli</b></span></a>
+
-
</div>
+
-
</br>
+
-
<section id="1st">
+
-
<div>
+
-
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
+
-
<h1>Overview</h1>
+
-
</br>
+
                $oe_menu_items.bind('mouseenter',function(){
-
</div>
+
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
   
   
-
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
+
$oe_menu.bind('mouseenter',function(){
-
</br>
+
var $this = $(this);
-
<span id="little"><span id="starter">W</span>hat we see and handle in lab is mostly ‘microscopic’. But there are macroscopic stuffs out there! At night, we can see stars, and among them, we can find Lion’s heart, ‘Regulus’. Although it is not the brightest kind, Regulus still makes it easy to notice the Lion in the sky. </span></br></br>
+
$this.addClass('hovered');
-
<span id="little2">Like Regulus, we want our module to be helpful for others to find their own Lion in this universe of synthetic biology. Our module may not be magnificent as that of other teams but we don’t actually want to excel others. We rather want to facilitate the advance of synthetic biology. Like the heart which beats in autonomous manner, our module will run by itself. Regarding all these aspects, we call our project <span style="color:#ff0000;"><b>‘Reguli’</b></span>.</br></br></span>
+
}).bind('mouseleave',function(){
-
<div clear='both'></div>
+
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items.children('div').hide();
 +
})
 +
            });
 +
</script>
 +
<script type="text/javascript">
 +
     
 +
$(function() {
 +
var $oe_menu2 = $('#oe_menu2');
 +
var $oe_menu_items2 = $oe_menu2.children('li');
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
                $oe_menu_items2.bind('mouseenter',function(){
-
+
var $this = $(this);
-
<span style="font-size:20px;font-weight:bold">Why does Autoregulation so necessary?</span></br></br>
+
$this.addClass('slided selected');
-
<span id="little">When we bring external pathway into E.coli to mass-produce the protein, it may collide with E.coli’s innate pathways by consuming critical resources which generate the redox potential (ATP, NADH, or NADPH molecules). This means that E.coli has to reach certain reducing potential to perform exotic metabolic pathway in the cell. </span></br></br>
+
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
-
<span id="little">In conventional methods, the promoters controlling protein expression are repressed until the cells get capacity to produce the protein. Then, this promoter can be induced by putting inducer to the solution on proper cell phase; cell phase can be measured by O.D. value. This kind of method was used because we assume that cell cultural Optical Density (which synchronizes with cell number in the culture) represents inner-cell reduction potential. Although in many time this method successfully makes cells to induce, there are several downsides in this method. </span></br></br>
+
$oe_menu_items2.not('.slided').children('div').hide();
-
<span id="little">First of all, problem arises with the process of adding inducers like IPTG or arabinose into media. Because the induction time is decided only by considering cell density (O.D.value) not the capacity of the cells (reduction potential), products may not come out as we predicted. Also, it is an annoying job to measure optical density, finding optimal induction conditions. Even more, if we miss the right time point, we might have to throw them away and start from the first place...</span></br></br>
+
$this.removeClass('slided');
-
<span id="little">This is why we came up with our auto-regulative device. As the result of iGEM project this summer, we suggest an auto-regulation module free from induction which utilizes dual-phase switching system.</span></br>
+
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
   
   
-
</div>
+
$oe_menu2.bind('mouseenter',function(){
-
</br>
+
var $this = $(this);
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
$this.addClass('hovered');
-
+
}).bind('mouseleave',function(){
-
<span style="font-size:20px;font-weight:bold">Auto-regulative device</span></br></br>
+
var $this = $(this);
-
<div align='center'><img src="https://static.igem.org/mediawiki/2012/8/83/KAIST_figure_overview_NUM1.png"/></div></br>
+
$this.removeClass('hovered');
-
<span id="little">With our automatically-regulative device above, we don’t have to do anything at all to induce gene. When the cells have the capacity to produce the target molecule, the pathway will be turn on. When the cell lost the capacity it will turn off. This is done by cell-source sensor and sensor-generated integrase gene. Integrase and excisionase invert and retrieve the recombination site which exists on the left and right side of promoter. After integrase gene inverts the promoter orientation, genes encoding bio-product pathway are transcribed, making bio-products. In addition, excisionase gene is expressed, reversing promoter orientation to its original state. </span>
+
$oe_menu_items2.children('div').hide();
-
</div>
+
})
-
</br>
+
            });
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
        </script>
-
+
 
-
<span style="font-size:20px;font-weight:bold">Environmental Impact</span></br></br>
+
 
-
<div align='center'><img src="https://static.igem.org/mediawiki/2012/c/ce/KAIST_figure_overview_NUM2.png"/></div></br>
+
-
<span id="little">Auto-regulating device can be further applied to make a noble sensing/degrading device for toxic compound or pollutant. When cells detect certain amount of toxic or pollutant, sensor induce integrase gene, thus invert the promoter sequence in between two recombination sites. After that, scavenger is generated degrading toxic/pollutant molecule. This novel sensing/scavenging device has its advantage in taking care of the environment and relieving the stress of host cell in automatic manner.</span>
+
-
</div>
+
-
+
-
</br></br>
+
-
+
-
<div align='right'><a href="#top">▲Back to the top</a></div>
+
-
+
-
+
-
</section>
+
-
</br></br>
+
-
<section id="2nd">
+
-
<div align='center'>
+
-
<img src="https://static.igem.org/mediawiki/2012/9/9f/KAIST_Proj_Overview.png"/></br></br>
+
-
</div>
+
-
As the name tells us, our new biobrick part <span style="color:#aa0000;"><b>FlipFlop(BBa_K907002 and BBa_K907003)</b> </span> is a noble device which can generate binary signal.</br></br>
+
-
<span id="little">This part is composed of three sub-parts.</br></br></span>
+
-
<div style="border:2px solid;padding:0px 20px 0px 20px;">
+
-
<ol style="padding:10px 0px 10px 30px;">
+
-
<li><b>Promoter</b> : <a href="http://partsregistry.org/Part:BBa_J23119">BBa_J23119</a></li>
+
-
<li><b>RBS</b> : <a href="http://partsregistry.org/Part:BBa_B0034">BBa_B0034</a></li>
+
-
<li><b>att sites</b> : Recognition site for <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase).</li>
+
-
</ol>
+
-
</div>
+
-
</br></br>
+
-
+
-
<div style="border:2px solid #0000aa;padding:0px 20px 20px 20px;">
+
-
<div style="color:#0000aa;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 0</span></div>
+
-
<div align='center'>
+
-
<img style="width:570px;" src="https://static.igem.org/mediawiki/2012/1/10/KAIST_Proj_Overview2.png"/>
+
-
</div>
+
-
</br>
+
-
Initially, the FlipFlop promotes the transcription and translation of down-stream gene Protein A according to its promoter orientation.
+
-
<div align='center'>
+
-
<br><img style="width:340px;" src="https://static.igem.org/mediawiki/2012/a/ae/KAIST_Proj_Overview3_2.PNG"/>
+
-
</div>
+
-
+
-
When <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked by attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.
+
-
</div>
+
-
</br></br>
+
-
+
-
<div style="border:2px solid #CC0000;padding:0px 20px 20px 20px;">
+
-
<div style="color:#CC0000;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 1</span></div>
+
-
<div align='center'>
+
-
<img src="https://static.igem.org/mediawiki/2012/1/1e/KAIST_Proj_Overview4.png"/>
+
-
</div>
+
-
</br>
+
-
Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step), or Protein B(Signal 1) in Figure 4.</br></br>
+
-
<div align='center'>
+
-
<img src="https://static.igem.org/mediawiki/2012/4/40/KAIST_Proj_Overview5.png"/>
+
-
</div>
+
-
</br>
+
-
Also, the signal can be restored to its initial state when <a href="http://partsregistry.org/Part:BBa_K907000">BBa_K907000</a>/ <a href="http://partsregistry.org/Part:BBa_K907001">BBa_K907001</a> (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.</br>
+
-
</div>
+
-
</br></br>
+
-
<div style="border:2px solid #0000aa;padding:0px 20px 20px 20px;">
+
-
<div style="color:#0000aa;font-size:25px;font-weight:bold;position:relative;top:-12px;"><span style="background-color:#fff;padding:0px 10px 0px 10px;">Signal 0</span></div>
+
-
<div align='center'>
+
-
<img src="https://static.igem.org/mediawiki/2012/3/32/KAIST_Proj_Overview6.png"/>
+
-
</div>
+
-
</div>
+
-
+
-
</br></br>
+
-
<div align='right'><a href="#top">▲Back to the top</a></div></br>
+
-
+
-
+
-
</section>
+
-
<br>
+
-
<br>
+
-
<br>
+
-
</br>
+
-
</br>
+
-
</br>
+
-
<section id="3rd">
+
-
<div>
+
-
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
+
-
<img src="https://static.igem.org/mediawiki/2012/9/99/KASIT_Lion2_igem.png" style="float:left;position: relative;z-index: 100;top: -75px;left:5px;">
+
-
<h1>Reguli</h1>
+
-
+
-
</br>
+
-
</div>
+
-
+
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
-
<div align='center'>
+
-
<img src="https://static.igem.org/mediawiki/2012/c/cc/KAIST_Overview_2nd_2.png"/>
+
-
</div>
+
-
</br>
+
-
Using the FlipFlop device, we can generate biological machine that controls the metabolism as we intended. We decided to call the system as "Auto-regulating system in E.coli", <span style="color:#aa0000;"><b>Reguli</b></span>. To demonstrate the idea, we applied the device to control bio-indigo production, which is catalyzed by bacterial flavin-containing monooxygenase(bFMO).</br>
+
-
+
-
</div>
+
-
</br></br>
+
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
-
<span style="font-size:20px;font-weight:bold">What is bFMO?</span></br></br>
+
-
<img src="https://static.igem.org/mediawiki/2012/c/c8/KAIST_Overview_2nd_3.png" style="float:right; padding: 10px 10px 10px 10px;"/>
+
-
Bacterial flavin-containing monooxygenase(bFMO) converts  indole into isatin, which is then sequentially catalyzed into indigoid compounds emitting indigo color.
+
-
</br>Because the enzyme utilizes the primary metabolite, we can easily notice the enzyme is working well or not.  For the following experiments, engineered bFMO gene from Methylophaga sp. Strain SK1 is kindly provided by Duhee Bang from Yonsei University, Republic of Korea.
+
-
+
-
<img src="https://static.igem.org/mediawiki/2012/b/b9/KAIST_Overview_2nd_4.png" style="padding: 30px 10px 10px 10px;"/>
+
-
+
-
+
-
</div>
+
-
+
-
</br></br>
+
-
<div style="border:2px solid #000000;padding:20px 20px 20px 20px;">
+
-
<span style="font-size:20px;font-weight:bold">Working scheme of Our Design</span></br></br>
+
-
<img src="https://static.igem.org/mediawiki/2012/e/eb/KAIST_System_1.png" style="padding: 10px 0px 10px 0px;"/>
+
-
<img src="https://static.igem.org/mediawiki/2012/3/3e/KAIST_pAutoSimple_working_scheme.png" style="padding: 10px 0px 10px 0px;"/>
+
-
<br>
+
-
<br>
+
-
</br>
+
-
</br>
+
-
<img src="https://static.igem.org/mediawiki/2012/8/8f/KASIT_System_2.png" style="padding: 10px 0px 10px 0px;"/>
+
-
<img src="https://static.igem.org/mediawiki/2012/d/d0/KAIST_pAuto_working_scheme.png" style="padding: 10px 0px 10px 0px;"/>
+
-
+
-
+
-
</div>
+
-
+
-
</br></br>
+
-
<div align='right'><a href="#top">▲Back to the top</a></div></br>
+
-
+
-
+
-
</section>
+
-
+
-
+
-
</div>
+
-
</div>
+
-
+
-
</body>
+
-
+
-
+
-
<head>
+
-
<title> Kaist Footer</title>
+
<style>  
<style>  
#kaist-footerbox
#kaist-footerbox
Line 1,162: Line 904:
   
   
</style>
</style>
-
</head>
+
</head>
-
+
<body>
-
<body>
+
<div id="main">
-
<div id="kaist-footerbox"><img id="footer-img" align="middle" src="https://static.igem.org/mediawiki/2012/e/ef/Footer_background_kaist.png"></img>
+
 
-
</div>
+
 
-
</body>
+
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
 +
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
 +
 
 +
 +
 +
<div class="oe_wrapper">
 +
<ul id="oe_menu" class="oe_menu">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
 +
<div style="top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
 +
</ul>
 +
<ul style="width:100px;margin:0px 5px 0px 5px;">
 +
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
 +
<div style="left:-85px;top:25px;"><!-- -112px -->
 +
<ul>
 +
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
 +
<div style="left:-182px;top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="oe_wrapper2">
 +
 
 +
<ul id="oe_menu2" class="oe_menu2">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
 +
<div style="top:21px;left:32px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
 +
<div style="left:-91px;top:30px;"><!-- -112px -->
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
 +
</ul>
   
   
-
</p>
+
</div>
-
<!--
+
</li>
-
NewPP limit report
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
-
Preprocessor node count: 19/1000000
+
<div style="left:-152px;top:21px;">
-
Post-expand include size: 120/2097152 bytes
+
<ul style="width:100px;height:19px;">
-
Template argument size: 0/2097152 bytes
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
-
Expensive parser function count: 0/100
+
</ul>
-
-->
+
<ul style="width:100px;height:19px;">
-
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
-
<!-- Saved in parser cache with key 2012_igem_org:pcache:idhash:9665-0!1!0!!en!2 and timestamp 20130816121218 -->
+
</ul>
-
<div class="printfooter">
+
<ul style="width:100px;height:19px;">
-
Retrieved from "<a href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">https://2012.igem.org/Team:KAIST_Korea/Project_Background</a>"</div>
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
+
</ul>
-
<div class="visualClear"></div>
+
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="contact-us">
 +
<div id="contact-us-title">2013 KAIST Korea</div>
 +
<div id="contact-us-information">
 +
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
 +
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
 +
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
</div>
</div>
-
    </div>
+
</div>
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR  -->
+
   
-
<div class="visualClear"></div>
+
<div class="iGEMLogo">
-
    <div id='footer-box' class='noprint'>
+
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
-
        <div id="footer">
+
</div>
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
+
</div>
 +
<div id="main">
 +
<ul class="menu">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea">Home</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Team">Team</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">Project</a>
 +
 
 +
<!-- <ul>
 +
<li><a href="#" class="documents">Documents</a></li>
 +
<li><a href="#" class="messages">Messages</a></li>
 +
<li><a href="#" class="signout">Sign Out</a></li>
 +
</ul>
 +
-->
 +
 +
</li>
 +
<li><a href="#">Labnote</a></li>
 +
<li><a href="#">Human Practice</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Safety">Safety</a></li>
 +
 +
</ul> <!-- end .menu -->
 +
 +
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
   
   
-
  <!-- Recentchanges is not handles well DEBUG -->
+
-
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
+
-
      title='Recent changes'>Recent changes</a></li>
+
<div id="kaistcontent">
   
   
-
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:KAIST_Korea/Project_Background"
+
</br>
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
+
<section id="1st">
 +
<div>
 +
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
 +
<h1>Project : Overview</h1>
 +
 +
</br>
 +
</div>
   
   
-
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:KAIST_Korea/Project_Background"
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
-
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
+
</br>
 +
<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
 +
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The <span id="starter">RAPTOR</span> (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
 +
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
 +
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
 +
</br></br></span>
 +
<div clear='both'></div>
 +
 +
 +
 +
 +
 +
</br></br>
 +
<div align='right'><a href="#top">▲Back to the top</a></div></br>
   
   
   
   
 +
</section>
   
   
-
                <li id="t-upload"><a href="/Special:Upload"
 
-
                  title="Upload files [u]" accesskey="u">Upload file</a>
 
-
                </li>
 
-
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 
-
                </li>
 
-
                <li><a href='/Special:Preferences'>My preferences</a></li>
 
-
            </ul>
 
-
        </div> <!-- close footer -->
 
-
        <div id='footer'>
 
-
    <ul id="f-list">
 
   
   
-
            <li id="t-print"><a href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;printable=yes"
+
</div>
-
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
+
-
            </li>
+
-
+
-
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;oldid=297918"
+
-
              title="Permanent link to this revision of the page">Permanent link</a>
+
-
            </li>
+
-
+
-
+
-
        <li id="privacy"><a href="/2012.igem.org:Privacy_policy" title="2012.igem.org:Privacy policy">Privacy policy</a></li>
+
-
        <li id="disclaimer"><a href="/2012.igem.org:General_disclaimer" title="2012.igem.org:General disclaimer">Disclaimers</a></li>
+
-
    </ul>
+
-
</div> <!-- close footer -->
+
-
    </div> <!-- close footer-box -->
+
-
+
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
+
</div>
</div>
-
<!-- Served in 0.228 secs. --></body>
+
<div id="kaist-footerbox"><img id="footer-img" align="middle" src="https://static.igem.org/mediawiki/2013/e/ef/Footer_background_kaist.png"></img>
-
+
</div>
 +
</body>
</html>
</html>

Latest revision as of 10:12, 23 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.




Retrieved from "http://2013.igem.org/Team:KAIST_Korea"