Team:UNITN-Trento/Notebook/Labposts/06/14

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{
{
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"date" : "2013-06-06",
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"date" : "2013-06-11",
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"author" : "caterina-michele-fabio"
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"author" : "thomas-emil-michele",
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"title" : "We have the parts!",
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"title" : "Purification, quantification and digestion of SAMsynthetase and pSB1C3",
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"content" : "<html> We performed the purification of <b>BBa_J45320</b>, <b>BBa_J45119</b>, <b>BBa_J45700</b> (obtained from NEB10&beta; colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center> <img src="https://static.igem.org/mediawiki/2013/f/f2/Tn-2013-Digestion_EcoRI_PstI.jpg" style ="width: 450px"> </center></html>}}<html><br/></html>",
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"content" : "We purified the PCR (third attempt) products of SAMsynthetase and pSB1C3 linearized vector following the Quick Reference protocol. After that, we quantified them using the nanodrop instrument. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>SAM Synthetase</th><th>pSB1C3</th></tr><tr><td>1</td><td>18,6 ng/ul</td><td>21,6 ng/ul</td></tr><tr><td>2</td><td>16,2 ng/ul</td><td>16 ng/ul</td></table></center></html>}}That's definetely not a good result, however we will continue working with them.",
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"tags" : "PchB-PchBA-BSMT1"
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"tags" : "SAMsynthetase"
}
}

Latest revision as of 07:42, 3 October 2013

{ "date" : "2013-06-11", "author" : "thomas-emil-michele", "title" : "Purification, quantification and digestion of SAMsynthetase and pSB1C3", "content" : "We purified the PCR (third attempt) products of SAMsynthetase and pSB1C3 linearized vector following the Quick Reference protocol. After that, we quantified them using the nanodrop instrument.

Quantification results
SampleSAM SynthetasepSB1C3
118,6 ng/ul21,6 ng/ul
216,2 ng/ul16 ng/ul
That's definetely not a good result, however we will continue working with them.", "tags" : "SAMsynthetase" }

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