Team:UNITN-Trento/Notebook/Labposts/06/02

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(Difference between revisions)
(Created page with "{ "date" : "2013-06-12", "author" : "caterina-michele", "title" : "miniprep SAMsynthetase+pSB1C3!", "content" : "We took the three inocula of <b>SAMsynthetase+pSB1C3</b> and ...")
 
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{
{
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"date" : "2013-06-12",
+
"date" : "2013-06-06",
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"author" : "caterina-michele",
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"author" : "viola-bruno",
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"title" : "miniprep SAMsynthetase+pSB1C3!",
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"title" : "Transformations of the LMU Munich plasmids for B.subtilis",
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"content" : "We took the three inocula of <b>SAMsynthetase+pSB1C3</b> and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then to verify if the colonies that we took contain the correct insert we perform the screening protocol and the gel eletrophoresis.",
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"content" : "Me and Bruno transformed the parts that LMU Munich sent us in NEB10beta cells line.The following transformation worked:Bba_K823022 (pSBBS4S)Bba_K823027 (pSBBS2E)Bba_K823023 (pSBBS1C)",
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"tags" : "SAMsynthetase"
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"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
}
}

Latest revision as of 09:32, 3 October 2013

{ "date" : "2013-06-06", "author" : "viola-bruno", "title" : "Transformations of the LMU Munich plasmids for B.subtilis", "content" : "Me and Bruno transformed the parts that LMU Munich sent us in NEB10beta cells line.The following transformation worked:Bba_K823022 (pSBBS4S)Bba_K823027 (pSBBS2E)Bba_K823023 (pSBBS1C)", "tags" : "pSBBs1C-pSBBs4S-pSBBs2E" }

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