Team:UNITN-Trento/Notebook/Labposts/06/53

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(Difference between revisions)

Latest revision as of 07:56, 3 October 2013

{ "date" : "2013-06-24", "author" : "caterina-michele", "title" : " Gel, gel, gel..of BBa_K1065101", "content" : "After the incula that Michele made on Sunday of araCpBAD with BBa_K1065100 (BMST1 in pSB1C3) we did the screening of the minipreps.Before obtaining a good result we tried three different gels.As you can see from these pictures (METTI IMMAGINI) the first two gels made from the same digestion were not lucky while the last, performed with different agarose concentration (1.5% instead of 1%) gave a nice result!

Screening BBa_K1065101

", "tags" : "BBa_K1065101" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-27",
+	"date" : "2013-06-24",
-	"author" : "emil",
+	"author" : "caterina-michele",
-	"title" : "Transformation of 2 GFPs and inocula of B. subtilis backbones",
+	"title" : " Gel, gel, gel..of BBa_K1065101",
-	"content" : "<html>Emil transformed two construct of the ancient Igem competition: both consist in a RBS with the GFP(E0040) and 2 terminators(B0010)(B0012) and are contained in bSB1A2</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|GFP|<html><center><table class=\"tn-sp-table\"><tr><th>ID</th><th>Quantification</th><th>RBS strength</th></tr><tr><td>BBa_E0240</td><td>108.5 ng/&micro;l</td><td>medium</td></tr><tr><td>BBa_E0840</td><td>129 ng/&micro;l</td><td>strong</td></tr></table></center></html>}}<html>Emil followed the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a> loading 150 ng of each sample.Then Emil plated the product on an Ampicillin plate.<br>Moreover Emil did the inocula (in LB + Ampicillin) of two backbones(BBa_823024 and BBa_823026) from the plates of Fabio and Bruno(2 inocula for each sample).</html>",
+	"content" : "<html>After the incula that Michele made on Sunday of araCpBAD with BBa_K1065100 (BMST1 in pSB1C3) we did the screening of the minipreps.Before obtaining a good result we tried three different gels.As you can see from these pictures (METTI IMMAGINI) the first two gels made from the same digestion were not lucky while the last, performed with different agarose concentration (1.5% instead of 1%) gave a nice result!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening BBa_K1065101|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7a/Tn-20130624-BMST1_ARAcpbad_PSB1C3.jpg\" height=\"300px\" /></center></html>}}",
-	"tags" : "BBa_823024-BBa_823026-BBa_E0240-BBa_E0840-B. subtilis"
+	"tags" : "BBa_K1065101"
 }