Team:UNITN-Trento/Notebook/Labposts/06/56

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(Difference between revisions)

Latest revision as of 07:56, 3 October 2013

{ "date" : "2013-06-25", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 8)!!", "content" : " today we miniprepped k823001, k823024 and k823026 and screened successfully (1500 ng digested for the promoter, 800 ng for the plasmids). WOOT!! we used a standard gel composition for the backbones and the “ brand new formula” for K823001. Thing is, the 2 plasmids seem to be inverted!!! Now we gotta figure out when the two of them have been inverted!!", "tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac " }

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 {
-	"date" : "2013-06-28",
+	"date" : "2013-06-25",
-	"author" : "emil",
+	"author" : "fabio-bruno",
-	"title" : "Purification of K823024 and K823026 and inocula of the GFPs E0840 and E0240",
+	"title" : "bacillus subtilis promoters and  plasmids (part 8)!!",
-	"content" : "<html>In orde to caracterize the inducible promoters of the two B. subtilis backbone I have to amplify two reporters and ligate them into the backbones. So I did the miniprep of  the 4 inocula(B.subtilis backbones) following the <a href=\"http://ita.promega.com/~/media/Files/Resources/ProtCards/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Quick%20Protocol.pdf\">vacuum protocol</a> then I have quantified the products with the following results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><table><tr><th>Sample</th><th>Quantification</th></tr><tr><td>BBa_K823024</td><td> 334.4ng/&micro;l</td></tr><tr><td>BBa_K823024</td><td> 260.6ng/&micro;l</td></tr><tr><td>BBa_K823026</td><td> 306.7ng/&micro;l</td></tr><tr><td>BBa_K823026</td><td> 359.7ng/&micro;l</td></tr></table></html>}}<html>Moreover I did the inocula of the 2 GFPs(BBa_E0240 and BBa_E0840)(4 inocula for each sample) from the plates previously done.</html>",
+	"content" : "<html> today we miniprepped k823001, k823024 and k823026 and screened successfully (1500 ng digested for the promoter, 800 ng for the plasmids). WOOT!! we used a standard gel composition for the backbones and the “ brand new formula” for K823001. Thing is, the 2 plasmids seem to be inverted!!! Now we gotta figure out when the two of them have been inverted!!</html>",
-	"tags" : "K823024-K823026"
+	"tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac "
 }