Team:UNITN-Trento/Notebook/Labposts/06/64

From 2013.igem.org

(Difference between revisions)

Latest revision as of 07:59, 3 October 2013

{ "date" : "2013-06-27", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 10)!!", "content" : " I miniprepped K823025 and obtained two good yields: 293,5 ng/ul and 236,6 ng/ul; I screened both samples (1000 ng digested in 50 ul) and the gel confirmed the presence of the plasmid. We have another one!!Afterwards we reported a summary table of part obtained and their yelds

Summary table

Sample	ng/ul
K823001 a	436.8
b	275.4
c	466.5
K823002a	138.0
b	134.4
c	139.7
K823003 a	85.4
b	99.9
c	118.9
K143012 a	178.3
b	89.2
c	121.4
K823024 a	362.1
d	209.3
e	288.2
K823026 a	246.3
b	315.8
c	550.3

", "tags" : "plasmidPxil-plasmidPspac - plasmidluxABCDE-Pveg-PliaG-PlepA-Plial" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-25",
+	"date" : "2013-06-27",
 	"author" : "fabio-bruno",
-	"title" : "bacillus subtilis promoters and  plasmids (part 8)!!",
+	"title" : "bacillus subtilis promoters and  plasmids (part 10)!!",
-	"content" : "<html> today we miniprepped k823001, k823024 and k823026 and screened successfully (1500 ng digested for the promoter, 800 ng for the plasmids). WOOT!! we used a standard gel composition for the backbones and the “ brand new formula” for K823001. Thing is, the 2 plasmids seem to be inverted!!! Now we gotta figure out when the two of them have been inverted!!</html>",
+	"content" : "<html> I miniprepped K823025 and obtained two good yields: 293,5 ng/ul and 236,6 ng/ul; I screened both samples (1000 ng digested in 50 ul) and the gel confirmed the presence of the plasmid. We have another one!!Afterwards we reported a summary table of part obtained and their yelds</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Summary table|<html><center><table border=\"1\"><tr><th>Sample</th><th>ng/ul</th></tr><tr><td>K823001 a</td><td>436.8</td></tr><tr><td>b</td><td>275.4</td></tr><tr><td>c</td><td>466.5</td></tr><tr><td>K823002a</td><td>138.0</td></tr><tr><td>b</td><td>134.4</td></tr><tr><td>c</td><td>139.7</td></tr><tr><td>K823003 a</td><td>85.4</td></tr><tr><td>b</td><td>99.9</td></tr><tr><td>c</td><td>118.9</td></tr><tr><td>K143012 a</td><td>178.3</td></tr><tr><td>b</td><td>89.2</td></tr><tr><td>c</td><td>121.4</td></tr><tr><td>K823024 a</td><td>362.1</td></tr><tr><td>d</td><td>209.3</td></tr><tr><td>e</td><td>288.2</td></tr><tr><td>K823026 a</td><td>246.3</td></tr><tr><td>b</td><td>315.8</td></tr><tr><td>c</td><td>550.3</td></tr></table></center></html>}}<html></html>",
-	"tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac "
+	"tags" : "plasmidPxil-plasmidPspac - plasmidluxABCDE-Pveg-PliaG-PlepA-Plial"
 }