Team:UNITN-Trento/Notebook/Labposts/07/55

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(Created page with "{ "date" : "2013-07-11", "author" : "emil", "title" : "The incompetent Bacillus subtilis saga:bacillus medium begins", "content" : "<html>Firstable I did an inoculum of Neb10...")
 
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{"date" : "2013-07-23","author" : "bruno","title" : "Blue sensor","content" : "<html>I made mini prep from inoculum overnight. I oubtained the following yields:<br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>K952003-1 </td><td>1019,1 ng/ul</td></tr><tr><td>K952003-2</td><td>1715,7 ng/ul</td></tr><tr><td>K952003-3 </td><td>180,0  ng/ul</td></tr><tr><td>K952003-4</td><td>236,4 ng/ul</td></tr></TABLE></center></html>}}<html><br/>from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.</html>","tags" : "BBa_K952003"}
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"date" : "2013-07-11",
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"author" : "emil",
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"title" : "The incompetent Bacillus subtilis saga:bacillus medium begins",
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"content" : "<html>Firstable I did an inoculum of Neb10&beta; in order to make them competent the next day.I started to prepare the media needed for the transformation of Bacillus according to LMU munich protocol but due to the failure of this protocol I won't report the protocol.</html>",
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"tags" : "Bacillus subtilis"
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}
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Latest revision as of 10:45, 3 October 2013

{"date" : "2013-07-23","author" : "bruno","title" : "Blue sensor","content" : "I made mini prep from inoculum overnight. I oubtained the following yields:

Quantification Results
SampleConcentration
K952003-1 1019,1 ng/ul
K952003-21715,7 ng/ul
K952003-3 180,0 ng/ul
K952003-4236,4 ng/ul

from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.","tags" : "BBa_K952003"}