Team:UNITN-Trento/Notebook/Labposts/07/63

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(Created page with "{ "date" : "2013-07-18", "author" : "thomas-michele", "title" : "Back to work", "content" : "<html>Today, after a long and hard fight with our thesis, we came back to work. I...")
 
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{"date" : "2013-07-27","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "<html> I first added sap to the o/n digestion( third), then purified and quantified : yield, 32,3 ng/ul.Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b.In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng/ul for 016 and 11,8ng/ul for oo6. Tomorrow I will continue with the ligation number 4.</html>","tags" : " YF1_FixJ - FixK2"}
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"date" : "2013-07-18",
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"author" : "thomas-michele",
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"title" : "Back to work",
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"content" : "<html>Today, after a long and hard fight with our thesis, we came back to work. In particular we teased the 2012 Registry Distribution and we extracted two new parts: BBa_K537055 (679 bp) and BBa_K537056 (721 bp). These two parts contained two reportes gene, mCherry and Venus. We did this because we wanted to fuse at the C-term of BBa_K1065001 a reporter in order to measure the kinetic of the protein sythesis. A new chapter to add to our team!  </html>",
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"tags" : "EFE-mCherry-Venus"
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}
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Latest revision as of 10:47, 3 October 2013

{"date" : "2013-07-27","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " I first added sap to the o/n digestion( third), then purified and quantified : yield, 32,3 ng/ul.Then again I ligated 006 to 016 folowing the protocol and plated 10 ul in 200 ul of neb10b.In the afternoon I made 6 inocula from the second ligation plates!Today I decided also to take a crack at a fourth ligation with a new strategy: using 006 as my plasmid and 016 as the insert!! I need to digest the two part with different enzymes E and X for 006 and E and S for 016! This time I digested 2400 ng for only 5 ours, not all the night: yields are, 25 ng/ul for 016 and 11,8ng/ul for oo6. Tomorrow I will continue with the ligation number 4.","tags" : " YF1_FixJ - FixK2"}

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