Team:UNITN-Trento/Notebook/Labposts/07/03

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(Difference between revisions)

Latest revision as of 10:32, 3 October 2013

{"date":"2013-07-01","author":"viola","title":"PCR of the double terminator BBa_B0015","content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:

	B0015 in pSB1AK3 219,4 ng/ µl	B0015 in pSB1C3 413,3 ng/ µl
H20	33.3 µl	33.3 µl
DNA	0.5 µl	0.5 µl
DNTPs	1 µl	1 µl
pref. FW	2.5 µl	2.5 µl
suff. RV	2.5 µl	2.5 µl
BUFFER	10 µl	10 µl
Phusion pol.	0.5 µl	0.5 µl

}}</html>\" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>

Gel image

on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.","tags":"EFE"}

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+{"date":"2013-07-01","author":"viola","title":"<html>PCR of the double terminator BBa_B0015</html>","content":"<html>Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities: <table> <tr> <th></th> <th>B0015 in pSB1AK3 <br/> 219,4 ng/ µl </th> <th>B0015 in pSB1C3 <br/> 413,3 ng/ µl </th></tr> <tr> <td>H20</td> <td>33.3 µl </td> <td>33.3 µl</td> </tr> <tr> <td>DNA</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> <tr> <td>DNTPs</td> <td>1 µl</td> <td>1 µl</td> </tr> <tr> <td>pref. FW</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>suff. RV</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>BUFFER</td> <td>10 µl</td> <td>10 µl</td> </tr> <tr> <td>Phusion pol.</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> </table> </html>}}</html>\" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/37/Tn-2013_Corsa20minPCR_B0015.jpg\" width=\"450px\" /></center></html>}}<html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.","tags":"EFE"}
-	"date" : "2013-07-18",
-	"author" : "emil",
-	"title" : "The incompetent Bacillus subtilis saga medium episode ",
-	"content" : "<html>I have prepared a minimal medium for bacillus following the Cambridge Igem 2012 protocol and I did an inocula of bacillus from a glicerol stock 50% for the following day.Moreover I tried to grow some competent NEB10&beta; following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-prep\"> competent cell protocol </a>.</html>",
-	"tags" : "Bacillus_subtilis"
-}