Team:Hong Kong HKU

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Bacterial Microcompartments (MCP) are closed polyhedral shells, diameter of 100-150nm, which is made of thin protein sheets, enclosing enzymes and cofactors for various forms of fermentative metabolism. Salmonella enteric enthanolamine ultilization (Eut) MCP is one example.
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5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell and empty recombinant Eut Shell has been successfully expressed in E.coli.  
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In addition, a localization signal (N terminal of EutC19) has been identified and signal fused enzymes/ proteins could be targeted to the cavity of the MCP.
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Inspired by these studies, we think this Eut MCP can become a versatile tool if we can:
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(a) Modify its exterior surface to enable surface display
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(b) Localize special enzyme into the MCP to enable specific metabolism
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(c) Store useful or harmful molecules into the MCP
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In this iGEM project, using Eut Microcompartment, we aim to show:
 
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(1) MCP shell surface can be used to display specific peptides, without disrupting the MCP structure, to enable cell-specific targeting for drug delivery system, easy purification etc. We will display Flag and His tag as a proof of concept.
 
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(2) Localize polyphosphate kinase (ppk1) into the native/ engineered MCP to polymerize inorganic phosphate and accumulate them in MCP, trying to treat phosphate pollution in waste water.
 
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We will divide into 2 parallel sub-teams, specifically working on surface decoration and ppk enzyme.
 
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Latest revision as of 13:08, 11 October 2014

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