Team:Berkeley

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                <li id="TitleID"> <a id="TitleID" href="https://2013.igem.org/Team:Berkeley">Project Blue Genes</a>
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                <li><a href="#1">Introductory Slides</a>
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        <div class="heading-large"><a>Project Blue Genes</a>
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                <div class="heading"><a >Project Description</a>
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                <p>The industrial dyeing process is ecologically unfriendly - indigo is made from an oil derivative (benzene) using harsh chemicals and extremely high temperatures. Furthermore, for indigo to actually adhere to fabric, a reducing agent is necessary to solubilize the dye, and this process releases acidified wastewaters into local environments. </p> <p>Research has shown that the leaves of indigo-producing plants contain two enzymes, a glucosyltransferase (GT) and a glucosidase (GLU), which together control indigo solubility by interconverting indigo with a soluble form called indican. We propose a biological dyeing method that harnesses this pathway in a bacterial chassis. </p><p> For the first time, indican has been produced using a recombinant GT as a result of our work. We prove indican irreversibly dyes fabric with the use of a GLU. Lastly, we have examined  ways to reduce our new system’s costs by optimizing indoxyl (an indican precursor) production and taking into account other innovative design choices.</p><p><b> Our work has created an entirely new way to dye denim with purely biological inputs which, with continued optimization, could compete economically with current industrial methods.</b>
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</p><p> <a href="https://2013.igem.org/Team:Berkeley/Project/Introduction">Read on</a> to learn more about our nature-inspired system!</p>
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                <div class="heading"><a name="Sponsors">&nbsp;&nbsp;Acknowledgements</a>
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                <p>The UC Berkeley iGEM team would like to thank Autodesk, Agilent, Synthetic Biology Institute, Qualcomm, Dean A. Richard Newton Memorial Chair for their financial support, IDT for discounted oligos and Quintara Bio for discounted sequencing service.</p>
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Some of the most major advances in synthetic biology involve the ability to use microorganisms to synthesize compounds that are difficult or costly to produce chemically. Our team hopes to exploit this familiar application of synthetic biology for the production of indigo in E. Coli.</p>
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Indigo, the very dye used to dye jeans around the world, is produced in quantities up to tens of thousands of tons every year. The chemical process involves converting vast quantities of aniline into indigo. Because indigo is extremely insoluble, this product must be reduced to leuco-indigo, a white soluble substance, using sodium dithionite. Upon immersing the cotton in leuco-indigo and exposing the coated cloth to air, indigo is allowed to reform, covalently bonding with the cloth. The wastes from this process are often discarded in large amounts - potentially altering the pH and stability of surrounding environments. Moreover, the process to produce analine is itself fraught with many unfriendly chemicals including sulfuric acid and nitric acid. With this chemical process in mind, our team seeks to improve titers of indigo using a bacterial system, thus bypassing several steps in the currently popular chemical synthesis process.
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Latest revision as of 03:51, 29 October 2013

Project Blue Genes
Project Description

The industrial dyeing process is ecologically unfriendly - indigo is made from an oil derivative (benzene) using harsh chemicals and extremely high temperatures. Furthermore, for indigo to actually adhere to fabric, a reducing agent is necessary to solubilize the dye, and this process releases acidified wastewaters into local environments.

Research has shown that the leaves of indigo-producing plants contain two enzymes, a glucosyltransferase (GT) and a glucosidase (GLU), which together control indigo solubility by interconverting indigo with a soluble form called indican. We propose a biological dyeing method that harnesses this pathway in a bacterial chassis.

For the first time, indican has been produced using a recombinant GT as a result of our work. We prove indican irreversibly dyes fabric with the use of a GLU. Lastly, we have examined ways to reduce our new system’s costs by optimizing indoxyl (an indican precursor) production and taking into account other innovative design choices.

Our work has created an entirely new way to dye denim with purely biological inputs which, with continued optimization, could compete economically with current industrial methods.

Read on to learn more about our nature-inspired system!

Follow us on

  Acknowledgements

The UC Berkeley iGEM team would like to thank Autodesk, Agilent, Synthetic Biology Institute, Qualcomm, Dean A. Richard Newton Memorial Chair for their financial support, IDT for discounted oligos and Quintara Bio for discounted sequencing service.

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