Team:DTU-Denmark/Notebook/21 August 2013

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{{:Team:DTU-Denmark/Templates/StartPage|21 August 2013}}
{{:Team:DTU-Denmark/Templates/StartPage|21 August 2013}}
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=lab 208=
=lab 208=
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==Main purpose==
==Main purpose==
<hr/>
<hr/>
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*USER reaction
 +
*running gels
==Who was in the lab==
==Who was in the lab==
<hr/>
<hr/>
 +
Kristian, Henrike
==Procedure==
==Procedure==
<hr/>
<hr/>
 +
 +
===USER ligation and transformation===
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 +
*with opened pZA21::ara and HAO, AMO, Cyc
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 +
*also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends
==Results==
==Results==
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* 1 kb ladder
* 1 kb ladder
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(pic big gel)
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[[File:2013-08-21 big screen.jpg|600px]]
* 1 kb ladder
* 1 kb ladder
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* 1 kb ladder
* 1 kb ladder
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(pic small gel)
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[[File:2013-08-21 small screen.jpg|600px]]
* 1 kb ladder
* 1 kb ladder
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* screening sample C12
* screening sample C12
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(pic small gel with big wells)
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[[File:2013-08-21 small screen 2.jpg|600px]]
==Conclusion==
==Conclusion==
<hr/>
<hr/>
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 +
PCR:
 +
*got pZA21::RFP:araBAD linearized and Sec2
 +
*the gradient PCR did not work
 +
Navigate to the [[Team:DTU-Denmark/Notebook/20_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/22_August_2013|Next]] Entry
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{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 17:48, 28 September 2013

21 August 2013

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Contents

lab 208


Main purpose


  • USER reaction
  • running gels

Who was in the lab


Kristian, Henrike

Procedure


USER ligation and transformation

  • with opened pZA21::ara and HAO, AMO, Cyc
  • also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends

Results


Gels

  • 1 kb ladder
  • pZA21::RFP:araBAD linearized
  • pZA21::RFP:araBAD linearized (duplicate)
  • test PCR Sec2
  • test PCR Sec2 (duplicate)
  • screening sample A4
  • screening sample A5
  • screening sample A6
  • screening sample A7
  • screening sample A8
  • screening sample A9
  • screening sample A10
  • screening sample A11
  • screening sample A12
  • screening sample B1
  • screening sample B2
  • screening sample B3
  • screening sample B4
  • 1 kb ladder

2013-08-21 big screen.jpg

  • 1 kb ladder
  • screening sample B5
  • screening sample B6
  • screening sample B7
  • screening sample B8
  • screening sample B9
  • screening sample B10
  • screening sample B11
  • screening sample B12
  • screening sample C1
  • screening sample C2
  • screening sample C3
  • screening sample C4
  • screening sample C5
  • 1 kb ladder

2013-08-21 small screen.jpg

  • 1 kb ladder
  • screening sample C6
  • screening sample C7
  • screening sample C8
  • screening sample C9
  • screening sample C10
  • screening sample C11
  • screening sample C12

2013-08-21 small screen 2.jpg

Conclusion


PCR:

  • got pZA21::RFP:araBAD linearized and Sec2
  • the gradient PCR did not work

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