Team:Uppsala/astraxantin

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      <h1 class="main-title-left">Astaxanthin</h1>
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<div id="molecule"> <div class="head"> <h1 > Molecule X </h1> </div>
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X
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<div id="methods"> <div class="head"> <h1>  Methods </h1> </div>
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        <img class="metabolic-pic" src="https://static.igem.org/mediawiki/2013/d/d8/Uppsala2013_AstaxanthinSymbol.png">
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Methods
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<p>Astaxanthin is a carotenoid in the carotenoid pathway that derives from the xanthophyll group and the precursor canthaxanthin is catalyzed by CrtZ. Astaxanthin is a very strong antioxidant but studies have shown that it also has other health benefits. The substance is created by many marine bacteria and microalgae and it gives the characteristic red color to many species such as salmon, lobster and shrimps.
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<br><br>
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Astaxanthin is an expensive part of salmon farming, used to give the meat its red color and it is approved in many countries as a food coloring with the e-number 161.[1] When astaxanthin is  produced it is usually by using the microalgae Haematococcus, or by producing it synthetically. The costs for astaxanthin today is around 2500 US dollars per kg, in total the market is worth around 200 million US dollars a year.[2] Since astaxanthin has many health benefits and that there is a demand for it on the market we decided to do more work on it to make it more available.</p>
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        <img class="molecule" src="https://static.igem.org/mediawiki/2013/4/47/Uppsala2013_Asthaxanthin-molecule.png">
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<div id="results">
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<a id="b1"></a><h1>Methods</h1>
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<p>To see that the bacteria containing the operon expressed astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted astaxanthin from salmon as a standard. After growing bacteria cultures with the operon we later used different extraction methods to extract the astaxanthin with various results.
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<br><br>
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<div class="head"> <h1> Results </h1> </div>
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We got the operon for producing astaxanthin from the Slovenian iGEM team 2010 which consisted of the genes CrtE, CrtB, CrtI, CrtY, CrtO and CrtZ with RBS, linkers, zinc fingers and the inducible promoter pBAD/AraC. We inserted the operon in e-coli DH5alpha. The zinc fingers aren´t of much interest to us so we won´t use them for any other parts.</p>
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Results
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      <img class="methods-pic" src="https://static.igem.org/mediawiki/2013/e/e5/Uppsala2013_Astaxanthin_plasmid_and_molecules.png">
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</div>  
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<img class="astaxanthin_plasmid_molecule" src=" ">
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<h1>Results</h1>
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</div>
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To see that the bacteria containing the operon expressed Astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted Astaxanthin from salmon as a standard. after growing bacteria cultures with the operon we later used different extraction methods to extract the Astaxanthin with various results.
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<div id="astaxanthin_extraction"> <img class="astaxanthin_extraction" src="https://static.igem.org/mediawiki/2013/c/ce/Uppsala2013_astaxanthin_pictures2.png"> <br>
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<i> Figure 1, mixed salmon that we boiled in methanol and centrifuged.  <br>
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      Figure 2, liquid liquid extraction where methanol was used as the eluent </i>
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</div>  
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<p>In the end we may have gotten a successful result but the extraction method was not good enough to get a clear graph proving the existence of astaxanthin, the extraction could only indicate that there is a possibility that we might have astaxanthin. See graph </p>
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graph coming soon
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Latest revision as of 20:25, 3 October 2013

Astaxanthin

Astaxanthin is a carotenoid in the carotenoid pathway that derives from the xanthophyll group and the precursor canthaxanthin is catalyzed by CrtZ. Astaxanthin is a very strong antioxidant but studies have shown that it also has other health benefits. The substance is created by many marine bacteria and microalgae and it gives the characteristic red color to many species such as salmon, lobster and shrimps.

Astaxanthin is an expensive part of salmon farming, used to give the meat its red color and it is approved in many countries as a food coloring with the e-number 161.[1] When astaxanthin is produced it is usually by using the microalgae Haematococcus, or by producing it synthetically. The costs for astaxanthin today is around 2500 US dollars per kg, in total the market is worth around 200 million US dollars a year.[2] Since astaxanthin has many health benefits and that there is a demand for it on the market we decided to do more work on it to make it more available.

Methods

To see that the bacteria containing the operon expressed astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted astaxanthin from salmon as a standard. After growing bacteria cultures with the operon we later used different extraction methods to extract the astaxanthin with various results.

We got the operon for producing astaxanthin from the Slovenian iGEM team 2010 which consisted of the genes CrtE, CrtB, CrtI, CrtY, CrtO and CrtZ with RBS, linkers, zinc fingers and the inducible promoter pBAD/AraC. We inserted the operon in e-coli DH5alpha. The zinc fingers aren´t of much interest to us so we won´t use them for any other parts.

Results

To see that the bacteria containing the operon expressed Astaxanthin we had to characterize them. To do that we used a spectrophotometer (nanodrop 2000 UV/Vis spectrophotometer) and we extracted Astaxanthin from salmon as a standard. after growing bacteria cultures with the operon we later used different extraction methods to extract the Astaxanthin with various results.


Figure 1, mixed salmon that we boiled in methanol and centrifuged.
Figure 2, liquid liquid extraction where methanol was used as the eluent

In the end we may have gotten a successful result but the extraction method was not good enough to get a clear graph proving the existence of astaxanthin, the extraction could only indicate that there is a possibility that we might have astaxanthin. See graph



graph coming soon

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