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Team:Macquarie Australia
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| - | <center> <h7> <p> The iGEM team at Macquarie University are aiming to introduce the genes necessary for chlorophyll production into <i>E.coli</i>. We at Macquarie are confident that we can make scientific strides in the understanding and construction of a photosynthetic bacterium. </p> | + | <!-- <center> <h7> <p> The iGEM team at Macquarie University are aiming to introduce the genes necessary for chlorophyll production into <i>E.coli</i>. We at Macquarie are confident that we can make scientific strides in the understanding and construction of a photosynthetic bacterium. </p> |
<br><p>Currently we have 2 out of 12 biobricks assembled and sequenced in our system, with work continuing on the remainder. </p> | <br><p>Currently we have 2 out of 12 biobricks assembled and sequenced in our system, with work continuing on the remainder. </p> | ||
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<br>Production of chlorophyll in <i>E.coli</i> would be the first steps towards the construction of photosystem II, a fundamental aspect of organic energy production. A better understanding of photosystem II opens the door to the production of harnessing green energy. | <br>Production of chlorophyll in <i>E.coli</i> would be the first steps towards the construction of photosystem II, a fundamental aspect of organic energy production. A better understanding of photosystem II opens the door to the production of harnessing green energy. | ||
| - | <p><br>If successful this would be the first successful production of chlorophyll within non-photosynthetic bacteria. </p> | + | <p><br>If successful this would be the first successful production of chlorophyll within non-photosynthetic bacteria. </p> --> |
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| + | <center><b><font size=5>Green is the new black - Expression of Chlorophyll within <i>Escherichia coli</i></font size></center></b> | ||
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| + | <h7> <p> Photosynthesis is a key biological pathway that uses sunlight energy to convert water and carbon dioxide into ATP, glucose <br> and oxygen. Chlorophyll is a green pigment that facilitates this energy production in photosynthetic organisms. Although the biosynthesis pathway for chlorophyll has been thoroughly investigated, the reproduction of this pathway in a non-photosynthetic organism has, to date, not been achieved.</p> | ||
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| + | <p>Successful production of chlorophyll in a bacterial host is the first step towards the synthetic construction of photosystem II, and the eventual creation of a renewable energy source. Our research involves expression of twelve genes (from <i>Chlamydomonas reinhardtii</i>) necessary for the chlorophyll biosynthesis pathway in a bacterial host (<i>E. coli</i>). </p> | ||
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| + | <p>Gene sequences have been synthetically designed to allow for prokaryotic expression. By utilising Gibson assembly, we plan on being able to successfully produce chlorophyll in prokaryotic cells. This will be evident from the growth of green <i>E. coli</i> colonies.</p> | ||
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| + | <center><b><font size=5>Our Gene Pathway</font size></center></b> | ||
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| + | <td><center><h7> <p> The figure below represents our proposed chlorophyll synthesis pathway within <i>E. coli</i>. Each gene is represented by blue and each chlorophyll precursor is coloured according to their visual colour shown on expression. Each gene sequence has been modified for codon optimization, whilst maintaining protein integrity.</p></h7> </center></font> | ||
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| + | <center>https://static.igem.org/mediawiki/2013/a/ae/Chloropyllpathway4.jpg</center> | ||
Latest revision as of 03:18, 28 September 2013
Photosynthesis is a key biological pathway that uses sunlight energy to convert water and carbon dioxide into ATP, glucose
and oxygen. Chlorophyll is a green pigment that facilitates this energy production in photosynthetic organisms. Although the biosynthesis pathway for chlorophyll has been thoroughly investigated, the reproduction of this pathway in a non-photosynthetic organism has, to date, not been achieved.
Successful production of chlorophyll in a bacterial host is the first step towards the synthetic construction of photosystem II, and the eventual creation of a renewable energy source. Our research involves expression of twelve genes (from Chlamydomonas reinhardtii) necessary for the chlorophyll biosynthesis pathway in a bacterial host (E. coli).
Gene sequences have been synthetically designed to allow for prokaryotic expression. By utilising Gibson assembly, we plan on being able to successfully produce chlorophyll in prokaryotic cells. This will be evident from the growth of green E. coli colonies.
The figure below represents our proposed chlorophyll synthesis pathway within E. coli. Each gene is represented by blue and each chlorophyll precursor is coloured according to their visual colour shown on expression. Each gene sequence has been modified for codon optimization, whilst maintaining protein integrity. |
