Template:Team:Uppsala/JS/notebook

Revision as of 11:22, 25 August 2013 (view source)

Krlu2935 (Talk | contribs)

← Older edit

Latest revision as of 23:26, 4 October 2013 (view source)

MikaelS (Talk | contribs)

(94 intermediate revisions not shown)

Line 1:

+

<html>

-

var month = ~~new Date().getMonth()~~;

+

var month = 8;

var year = new Date().getFullYear();

-

var cal_table = '';

+

var cal_table = '';

+

var eventString;

var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);

+

var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');

+

var inputs = 106;

Date.prototype.getWeek = function()

Line 17:

Line 21:

var today = date.getDate();

cal_table = '';

+

var eventString;

if(year<=200)

Line 26:

Line 31:

days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);

-

if(year%4 == 0 ~~&& year!=1900~~)

+

if(year%4 == 0)

{

-

days_in_month[1]=29;

+

if( year!=1900 )

-

}

+

{

+

days_in_month[1]=29;

+

}

+

}

total = days_in_month[month];

var date_today = months[month]+' '+year;

-

cal_table = cal_table + '<table class="cal_calendar" ><tbody id="cal_body"><tr class="head"><th class="arrow"><input id="left" type="image" onclick="left_arrow()" src="~~image~~/~~buttons~~/~~arrowleft~~.png"></th><th colspan="6" >'+date_today+'</th><th class="arrow"><input id="right" type="image" onclick="right_arrow()" src="~~image~~/~~buttons~~/~~arrowright~~.png"></th></tr>';

+

cal_table = cal_table + '<table class="cal_calendar" ><tbody id="cal_body"><tr class="head"><th class="arrow"> <input id="left" type="image" onclick="left_arrow()" src="https://static.igem.org/mediawiki/2013/a/a1/Arrowleft.png"></th><th colspan="6" >'+date_today+'</th><th class="arrow"><input id="right" type="image" onclick="right_arrow()" src="https://static.igem.org/mediawiki/2013/0/0b/Arrowright.png"></th></tr>';

cal_table = cal_table + '<tr class="cal_d_weeks"><th>Mon</th><th>Tue</th><th>Wed</th><th>Thu</th><th>Fri</th><th>Sat</th><th>Sun</th><th>Week</th></tr><tr>';

week = 0;

Line 53:

Line 61:

}

-

if(~~today==i && month == date.getMonth() &&~~ year == date.getFullYear())

+

if(year == date.getFullYear())

{

-

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="cal_today"><input type="submit" onclick="" value="'+i+'"></td>';

+

if(month == date.getMonth()){

+

if(today==i){

+

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="cal_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';

+

}

+

else{

+

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';

+

}

+

}

+

else{

+

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';

+

}

else

{

-

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="" value="'+i+'"></td>';

+

cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';

}

-

+

-

week++;

+

week++;

+

markerNotes(year, m, i);

if(week==7)

{

var weeknr = new Date(year, month, i-1).getWeek();

-

cal_table = cal_table + '<td class="week"><input type="submit" onclick="" value="'+weeknr+'"></td>';

+

cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';

cal_table = cal_table + '</tr>';

week=0;

Line 89:

Line 108:

var weeknr = weeknr+1;

}

-

cal_table = cal_table + '<td class="week"><input type="submit" onclick="" value="'+weeknr+'"></td>';

+

cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';

cal_table = cal_table + '</tr>';

week=0;

Line 96:

Line 115:

cal_table = cal_table + '</tbody></table>';

}

+

function left_arrow()

+

{

+

month = month-1;

+

if(month == -1)

+

{

+

year = year-1;

+

month = 11;

+

}

+

calendar();

+

document.getElementById('calendar').innerHTML = cal_table;

+

}

+

function right_arrow()

+

{

+

month = month+1;

+

if(month == 12)

+

{

+

year = year+1;

+

month = 0;

+

}

+

calendar();

+

document.getElementById('calendar').innerHTML = cal_table;

+

}

+

function showNotes(y, m, day)

+

{

+

var m = month+1;

+

eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

+

document.getElementById('file_holder').innerHTML = eventString;

+

document.getElementById('calendar').style.marginBottom="350px";

+

}

+

function showNotesOfweek(y, wnr)

+

{

+

var d = new Date(y, 0, 0);

+

if(y == 2012 ){

+

wnr++;

+

}

+

var w = d.getTime() + 604800000 * (wnr-1);

+

var m = new Date(w).getMonth()+1;

+

var y = new Date(w).getFullYear();

+

var day = new Date(w).getDate();

+

var e = new Date(w).getDay();

+

day = day - e+1;

+

eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

+

for(i=1;i<7;i++)

+

{

+

if(days_in_month[m-1] == day )

+

{

+

day = 0;

+

m++;

+

if(m>12)

+

{

+

y++;

+

var d = new Date(y, 0, 0);

+

var w = d.getTime() + 604800000 * (wnr-1);

+

var m = 1;

+

}

+

}

+

day++;

+

eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

+

}

+

document.getElementById('calendar').style.marginBottom="350px";

+

document.getElementById('file_holder').innerHTML = eventString;

+

eventString = '';

+

}

+

function markerNotes(y, m, d)

+

{

+

var id='t'+y+m+d;

+

for(c = 0; c<=inputs; c++)

+

{

+

if(days_with_dirys[c] == id)

+

{

+

cal_table = cal_table + '<style type="text/css">#'+id+'{border: 2px solid #0089d1;}</style>';

+

}

+

}

+

}

+

function diry(y, m, d)

+

{

+

var ds = ''; //diryString

+

var id = 'd'+y+m+d;

+

//var ds = y+'-'+m+'-'+d;

+

if(id == 'd2013630')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1>Name of participants: Magnus B, Alexander W, Nils A, Mikael S, Anders E <h2>Ongoing constructs:</h2>E. coli: 75. pEL3A15 - CrtB 25. pSB1C3-CrtY 27. pEL3K16-red 51.pEL3C18-red 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 L. reuteri: Lb1. CF48-pLR581 Lb2. 1063-pLUL631 Lb3. DSM 20016-pVs2 Lb4. 100-23-noplasmid Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP Lb8. 100-23-pGT232 Lb12. DSM 20016-noplasmid Lb14. DSM 20016-pLUL631 L. plantarum: Lb5. 256-rifR-pAMβ1 L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus Lb16. MG1363-noplasmid Lb10. 256-noplasmid Lb11. 36E-noplasmid Lb9. JH2-2 pAMβ1 <h2>Todays work:</h2> Transformation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red 75. pEL3A15 - CrtB Digest 27. pEL3K16-red 51.pEL3C18-red Ligation 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly. O/N Screening 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results</h2> Re-streak Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed </div>';

+

}

+

else if(id == 'd2013629')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1> Name of participants: Magnus B, Alexander W, Nils A, Anders E, Mikael S <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli (strain D5α): 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 57.B0034-Idi 75. pEL3A15-CrtB 76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Todays work</h2>PCR purification: CP1 CP8 CP11 CP29 CP30 CP41 Cp44 Digestion: 27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) Ligation: 24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red Transformation: 75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. Re-streak: 1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam Plasmid preparation: 42. pSB4S15-red O/N Screening PCR: 25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF (19,20,21(with DMSO dilution series(7,9,12)) PCR (screening?): 57.B0034-Idi IspA Gel electrophoresis: 57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) 27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) <h2>Results</h2>Today: Plasmid preparation: 42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL 42.4 pSB4S15-red klon 4: 25.3 ng/µL Gel electrophoresis: 57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow 23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow 24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow Transformation: 75. pEL3A15 - CrtB failed PCR purification (of promotors):* CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl *PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning). Previous day: ON screening: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions </div>';

+

}

+

else if(id == 'd2013628')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1>Name of participants: Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W <h2>Ongoing constructs:</h2>L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57.B0034-Idi ispA <h2>Todays work</h2>Frozen stock: Lb13. Lc. lactis MG1363 pJP059 clone 2 Lb15. Lc. lactis unknown pGus clone 2 Lb16. Lc. lactis MG1363 noplasmid clone 2 Digest: 25.pSB1C3-CrtY Transformation: 24.pSB1C3-CrtI 23.pSB1C3-CrtB Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)” Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)” 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Re-streaks: Lb3. reuteri DSM20016 pVS2 Lb8. reuteri 100-23 pGT232* Lb11. plantarum 36E Lb13. Lc. lactis MG1363 pJP059 Lb16. Lc. lactis MG1363 noplasmid *from frozen stock Streaking of strains: Lb15. Lc. lactis MG1363 plasmid unknown pGus: Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) O/N of restreak from Erik Gullberg: 42. pSB4S15-red, clones 1 and 2 PCR: 57.B0034-Idi* ispA* 19.1** 20.1** 21.1** * Amplified from D5α-chromosome. ** Now with DMSO Gel electrophoresis: 57.B0034-Idi ispA 19.1 20.1 21.1 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N Screening: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 <h2>Results</h2>Previous day: Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4) → Store in freezer, try again with new protocol Transformation: Lb10. plantarum 256 pLUL364: No growth Lb11. plantarum 36E pVS2: No growth Lb12. Lc. lactis DSM20016-pVS2: No growth → Try again later with new competent cells Wednesday 26/6: Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today Today: Plasmid preparation: Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl → Mark and store in freezer. Gel electrophoresis: 57.B0034-Idi: hard do decipher results, redo tomorrow ispA: (“) 19.1: failed 20.1: failed 21.1: failed 43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: failed (“) 45. pSB1C3-B0034-4CL: failed (“) 46. pSB1C3-B0034-His-4CL: failed (“) 47. pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) <h2>Other experiments</h2>THMS buffer preparation Aliquoting of sigma water Preparation of antibiotic-free LB-agar plates Dilution of promotor-oligonucleotides from stock to 5 µM: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R Dilution of promotor oligonucleotide from pellet to 100 µM: usp45_mut_F Phosphorylation of promotor-oligonucleotides: CP1_F CP1_R CP11_F CP11_R CP8_F CP8_R CP44_F CP44_R CP30_F CP30_R CP29_F CP29_R CP41_F CP41_R</div>';

+

}

+

else if(id == 'd2013627')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1> Name of participants: Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS <h2>Todays work</h2> Transformation: Lb11. plantarum 36E pVS2 Lb10. plantarum 256 pLUL634 Lb12. plantarum DSM20016 pVS2 Assembly: 23.Crt.B + pEL3A15 24.Crt.I + pEL3K16 Frozen stock: Lb9. faecalis JH2-2 pAMβ1 Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb16. Lc. lactis MG1363 no plasmid clone 1 Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb9. faecalis JH2-2 pAMβ1 Over night: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19. (digest from previous day) 20. (“) 21. (“) PCR amplification of construct: miraculin 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25. pSB1C3-CrtY 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N screening PCR: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25.pSB1C3-CrtY <h2>Results</h2> Previous day: O/N: Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! * Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!* Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! ** * Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day ** → Frozen stock and plasmid prep. Transformation: 42. pSB4S15-red: Many small white colonies, contamination with cocci? → Discard, get clones from Erik G. Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Some growth. Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock. Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock. → let grow one more day. Today: Plasmid preparation: Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 1: 4.9 ng/µl (run 1) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3) Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1) → Measure again tomorrow Gel electrophoresis: Miraculin: fail, problems with primers, probably due to problematic secondary structure. 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27) 19. (digest from previous day): Success 20. (“): Success 21. (“): Success 19. (PCR): Fail -> redo with lower TM 20. (“): Fail -> redo with lower TM 21. (“): Fail -> redo with lower TM 25. pSB1C3-CrtY: Good results <h2>Other experiments</h2> Preparation of THMS buffer</div>'

+

}

+

else if(id == 'd2013626')

+

{

+

ds = '<div id="dairy-text"><h2>Wednesday 2013-06-26</h2> Name of participants: Christoffer A, Stephanie H, Alona N, Anders E, Mikael S, Anton B, Viktor B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Theodor L, Magnus B., Malin B., Thorsteinn O., Nils A., Alexander W., Niclas S., Pontus D., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 25.pSB1C3-CrtY 19.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.psB4C5-CrtE~I / w. linker, ZF & RBS 42. D5α with plasmid pSB4S15-red <h2>Todays work</h2> Competent cells preparation: Lb11. plantarum 36E no plasmid Lb10. plantarum 256 no plasmid Lb12. reuteri DSM 20016 no plasmid Transformation: plantarum 36E pAMB1 plantarum 256 pAMB1 Lb3. reuteri DSM 20016 pVS2 25.pSB1C3-CrtY 42. pSB4S15-red O/N: Lb5.2 plantarum 256 pAMβ1* Lb8.2 reuteri 100-23 pGT232* Lb13. Lc. lactis MG1363 pJP059** Lb15. Lc. lactis MG1363 pGus** Lb16. Lc. lactis MG1363 no plasmid** *Two tubes of 8.2 were prepared. It has grown rather poorly to not at all before. If it continues to grow poorly we may have to redo O/N from a plate rather than from our frozen stock. ** Optimized conditions → 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. Digestion: 19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS 20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.1.psB4C5-CrtE~I / w. linker, ZF & RBS 21.2.psB4C5-CrtE~I / w. linker, ZF & RBS Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT PCR amplification of construct: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (third try, using same concentration of template as second try but lower annealing temperature ) 19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS 19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS 20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS 20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS 21.1.psB4C5-CrtE~I / w. linker, ZF & RBS 21.2.psB4C5-CrtE~I / w. linker, ZF & RBS <h2>Results</h2> Previous day: O/N: Lb13. Lc. lactis MG1363 pJP059: Growing, visual pellet.* Lb15. Lc. lactis MG1363 pGus: Growing, visual pellet.* Lb16. Lc. lactis MG1363 no plasmid: Growing, visual pellet.* *Weak growth in the morning, better in the afternoon. → Let grow for one more day + new O/N with optimized conditions. Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: No visual growth → Let grow for one more day. Lb15. Lc. lactis MG1363 pGus: No visual growth → --”-- Lb16. Lc. lactis MG1363 no plasmid: No visual growth → --”— Today: Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: Failed, possibly nonspecific primer binding, not enough template DNA. Image 1 (2013.06.26) Transformation: 25.pSB1C3-CrtY: failed D5alpha competent cells were competent <h2>Other experiments</h2> Preparation of cold electroporation buffer for competent cells Preparation of 50% glycerol stock: 500 ml 50% glycerol stock was prepared from 86-88% glycerol.</div>'

+

}

+

else if(id == 'd2013625')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1> Name of participants: Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Gel electrophoresis: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m PCR amplification of construct: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature ) Lb13. Lc. lactis MG1363 pJP059 Competent cells preparation: Lb11. plantarum 36E no plasmid Lb10. plantarum 256 noplasmid Lb12. reuteri DSM 20016 no plasmid Plasmid preparation: Lb6.1 reuteri DSM 20016 pLUL634TsA8 Lb6.2 reuteri DSM 20016 pLUL634TsA8 Lb6.3 reuteri DSM 20016 pLUL634TsA8 Lb6.4 reuteri DSM 20016 pLUL634TsA8 * Lb7.2 reuteri DSM 20016 pGFP Lb7.3 reuteri DSM 20016 pGFP Lb7.4 reuteri DSM 20016 pGFP * Lb14.1 reuteri DSM 20016 pLUL631 Lb14.2 reuteri DSM 20016 pLUL631 Lb14.3 reuteri DSM 20016 pLUL631 Lb14.4 reuteri DSM 20016 pLUL631 * * 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml. O/N: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid Streaking of strains: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid PCR amplification: Lb13. Lc. lactis MG1363 pJP059* *Another attempt at colony PCR with different concentrations of lysozyme and buffer. <h2>Results</h2> Gel electrophoresis: 15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25) Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture. Competant cells: Wrong volume was added. → Try again tomorrow with the right volume. Plasmid preparation: * Lb6.1 reuteri DSM 20016 pLUL634 TsA8: 203.7 ng/µl Lb6.2 reuteri DSM 20016 pLUL634 TsA8: 203.2 ng/µl Lb6.3 reuteri DSM 20016 pLUL634 TsA8: 154.8 ng/µl Lb6.4 reuteri DSM 20016 pLUL634 TsA8: 39.5 ng/µl Lb7.2 reuteri DSM 20016 pGFP: 100.5 ng/µl Lb7.3 reuteri DSM 20016 pGFP: 141.2 ng/µl Lb7.4 reuteri DSM 20016 pGFP: 8.7 ng/µl Lb14.1 reuteri DSM 20016 pLUL631: 162.7 ng/µl Lb14.2 reuteri DSM 20016 pLUL631: 85.2 ng/µl Lb14.3 reuteri DSM 20016 pLUL631: 49.8 ng/µl Lb14.4 reuteri DSM 20016 pLUL631: 7.7 ng/µl *The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones. <h2>Other experiments</h2> Preparation of MRS broth Making plates for Lc. lactis cultivation (from M17-broth) Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</div>'

+

}

+

else if(id == 'd2013624')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1> Name of participants: Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 E. coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Plasmid preparation: Lb8. reuteri 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 Digestion of construct: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m O/N (from frozen stock): Lb2. 1063 pLUL631 Lb6. DSM 20016 pLUL63TsA8 Lb7. DSM 20016 pGFP Lb8. 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 PCR: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT* Lb13. Lc. lactis MG1363 pJP059* *Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: 1.1. pSB1C3-red 18.2. pSB1C3-CHS_m 40. pEL3A17-red <h2>Results</h2> Plasmid preparation: Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today. <h2>Other experiments</h2> Primer stock preparation Dilution of sequencing primers: 3 tubes of 5 µM VF2-primer, 200 uL 3 tubes 5 µM VR-primer, 200 µL Re-measuring concentration of earlier plasmid preps: * 1.4 CF48-pLR581: 127.8 ng/µl 1.5 CF48-pLR581: 215.9 ng/µl 3.2 DSM 20016-pVs2: 201.2 ng/µl 3.3 DSM 20016-pVs2: 139.1 ng/µl 3.10 DSM 20016-pVs2: 183.5 ng/µl *We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind. Preparing M17-broth, 950 ml. (“) SOB (“) CCMB80 (20% glycerol, pH 6,8) Alliquoting dNTP, buffer, enzymes</div>'

+

}

+

else if(id == 'd2013623')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1> Name of participants: Hampus E, Alexander W <h2>Ongoing constructs:</h2> L. reuteri: 4. 100-23-noplasmid L. plantarum: 5. 256-rifR-pAMβ1 E. coli: 23.pCrcSB1C3-Crt-B 24.pSB1C3-Crt-I 25. pSB1C3-CrtY <h2>Todays work</h2> Glycerol stock preparation: Lb4. reuteri 100-23 no plasmid Lb5. plantarum 256 rifR-Pamβ1 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I Restreak: 25. pSB1C3- crtY Plasmidpreparation 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I <h2>Result</h2> O/N: 25.pSB1C3-crtY: One of the overnights did not grow </div>'

+

}

+

else if(id == 'd2013622')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-06-22</h1> Name of participants: Nafisa B, Mikael S, Marcus H <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Glycerol stock preparation: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb8. reuteri 100-23-pGT232 Lb9. faecalis JH2-2- pAMβ1 Lb10. plantarum 256-noplasmid Lb11. plantarum 36E-noplasmid Lb12. reuteri DSM 20016-noplasmid Lb14. reuteri DSM 20016-pLUL631 (B?) O/N: Lb4. reuteri100-23-noplasmid Lb5. plantarum 256-rifR-pAMβ1 Plasmid preparation: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb14. DSM 20016-pLUL631 (B?) Lb6. DSM 20016-pLUL63TsA8 Lb7. DSM 20016-pGFP <h2>Results</h2> Plasmid preparation: L. reuteri: Lb14.1 (strain 14, clone 1) DSM 20016-pLUL631 (B?): 10.0 ng/μl Lb14.2 DSM 20016-pLUL631 (B?): 13.6 ng/μl Lb14.3 DSM 20016-pLUL631 (B?): 14.7 ng/μl Lb14.4 DSM 20016-pLUL631 (B?): 28,4 ng/μl Lb6.1 DSM 20016-pLUL63TsA8: 18.7 ng/μl Lb6.2 DSM 20016-pLUL63TsA8: 14.4 ng/μl Lb6.3 DSM 20016-pLUL63TsA8: 12.5 ng/μl Lb6.4 DSM 20016-pLUL63TsA8: 27.7 ng/μl Lb7.2 DSM 20016-pGFP: 29.9 ng/μl Lb7.3 DSM 20016-pGFP: 16.9 ng/μl Lb7.4 DSM 20016-pGFP: 10.9 ng/μl</div>'

+

}

+

else if(id == 'd2013621')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-06-21</h1> Name of participants: Stephanie H, Alona N, Anton B, Mikael S, Nafisa B, Anders E, Marcus H, Ken B-A, <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP <h2>Todays work</h2> Plasmid preparation*: Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 *to get more useable material and to look for different result from clones grown in a lock and a CO2-cupboard. O/N: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb14. reuteri DSM 20016-pLUL631(B?) Lb12. reuteri DSM 20016-noplasmid Lb8. reuteri 100-23-pGT232 Lb10. plantarum 256-noplasmid Lb11. plantarum 36E-noplasmid Lb9. faecalis JH2-2 pAMβ1 *- *from the plate without erythromycin. Plasmid preparation: 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP <h2>Results</h2> Previous day: Re-streak: Lb4. reuteri 100-23-noplasmid: No growth. → Grow on non-antibiotic plates Today: Plasmid preparation: Lb1.2 (strain 1, clone 2) reuteri CF48-pLR 581: Non-usable concentration * Lb1.4 reuteri CF48-pLR 581: 161.8 ng/µl * Lb3.8 reuteri DSM 20016 pVS2: 148.5 ng/µl * Lb3.10 reuteri DSM 20016 pVS2: Non-usable concentration * *there is seemingly no correlation between growth in lock vs. CO2-cupboard and plasmid concentration. This seems strange though. Cupboard is not supposed to create an anaerobic environment → do not use cupboard <h2>Other experiments</h2> SET buffer preparation: Lysis buffer preparation: </div>'

+

}

+

else if(id == 'd2013620')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24. pSB1C3-CrtI 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work:</h2> Glycerol stock preparation: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Plasmid preparation: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 26. pEL3K15-red 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 O/N: Lb2. reuteri 1063-pLUL631 Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 Streaking of new strains from Stefan Roos: Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb12. reuteri DSM 20016-noplasmid * Lb14. reuteri DSM 20016-pLUL631(B?) Lb4. reuteri 100-23-noplasmid Lb8. reuteri 100-23-pGT232 * Lb5. plantarum 256-rifR-pAMβ1 Lb10. plantarum 256-noplasmid * Lb11. plantarum 36E-noplasmid * Lb9. faecalis JH2-2 pAMβ1 ** *both on burned broth without glucose and CaCl2 and regular one. **re-streaked on erythromycin and on antibiotic-free plates. Transformation: 23. pSB1C3-CrtB 24. pSB1C3-CrtI Restreaks: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red <h2>Results</h2> Previous day: Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17) Today: Plasmid preparation: 19.1: 50.5 ng/µl 19.2: 33.6 ng/µl 19.3: 45.2 ng/µl 19.4: 36.5 ng/µl 20.1: 28.9 ng/µl 20.2: 29.6 ng/µl 20.3: 41.8 ng/µl 20.4: 35.8 ng/µl 21.1: 47.7 ng/µl 21.2: 23.2 ng/µl 21.3: 38.8 ng/µl 21.4: 48.9 ng/µl Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again. Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again. Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl <h2>Other experiments</h2> Antibiotic-free MRS-broth plates preparation: SET-buffer and Lysis-buffer preparation: MRS-broth agar preparation: Preparation of plates with and without antibiotic: </div>'

+

}

+

else if(id == 'd2013619')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1> Name of participants: Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A <h2>Ongoing constructs:</h2> L. reuteri: 2. 1063-pLUL631 1. CF48-pLR581 3. DSM20016-pVs2 Lactoccocus lactis: 13. MG1363-pJP059 E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work</h2> Re-streak: 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Spreading: Lb13. Lc. lactis MG1363-pJP059 O/N: 14. pET13b-zifz:4cl-PBSII:STS 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Transformation: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY <h2>Results</h2> Previous day: Spreading: Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. Transformation 1. pSB1C3-red: Successful 2. pSB3C5-red: Successful 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: Successful 7. pSB3K3-red: fail 12. pSB4K5-red: fail Restreak: 16. pSB1C3-TAL_m: Successful 17. pSB1C3-4Cl_m: Successful 18. pSB1C3-CHS_m: Successful 22. pSB1C3-B0032-BFP: Successful Today: O/N: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth Transformation: 23. pSB1C3-CrtB: fail 24. pSB1C3-CrtI: fail 25. pSB1C3-CrtY: fail <h2>Other experiments</h2> LB-agar plates CaCl2-solution</div>'

+

}

+

else if(id == 'd2013618')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1> Name of participants: Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B <h2>Ongoing constructs:</h2> L. reuteri: 2. 1063-pLUL631 1. CF48-pLR581 3. DSM20016-pVs2 Lactoccocus lactis: 13. MG1363-pJP059 E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP <h2>Todays work:</h2> Streaking of strains from Stefan Roos: Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Lb13. Lc. lactis MG1363-pJP059 Transformation 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP Restreak 14. pET13b-zifz:4cl-PBSII:STS 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results:</h2> Previous day Restreak: 14. pET13b-zifz:4cl-PBSII:STS: Growth Today Transformation: 1. pSB1C3-red: fail 2. pSB3C5-red: fail 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: fail 7. pSB3K3-red: fail 12. pSB4K5-red: fail 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail 16. pSB1C3-TAL_m: successful 17. pSB1C3-4Cl_m: successful 18. pSB1C3-CHS_m: successful 22. pSB1C3-B0032-BFP: successful Restreak: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth <h2>Other experiments:</h2> LB-agar</div>'

+

}

+

else if(id == 'd2013617')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1> Name of participants: Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F <h2>Ongoing constructs:</h2> E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Todays work:</h2> Transformation 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF <h2>Results:</h2> Today: Transformation: 1. pSB1C3-red: fail 2. pSB3C5-red: fail 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: fail 7. pSB3K3-red: fail 12. pSB4K5-red: fail 14. pET13b-zifz:4cl-PBSII:STS: successful 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m: fail 17. pSB1C3-4Cl_m: fail 18. pSB1C3-CHS_m: fail 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful <h2>Other experiments:</h2> Mixing MRS-broth Making of EDTA solution MRS-plates preparation</div>'

+

}

+

else if(id == 'd2013613')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-06-13</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F. <h2>Other experiments</h2> Making competent D5α</div>'

+

}

+

else if(id == 'd2013612')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-06-12</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F., Pontus D <h2>Other experiments</h2> LB-agar plates</div>'

+

}

+

else if(id == 'd2013610')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-06-10</h1> Name of participants: Alexander W, Magnus B, Malin B, Thorsteinn O, Nils A, Christoffer F., Pontus D, Niclas S. <h2>Other experiments:</h2> LB CaCl2 Antibiotics</div>'

+

}

+

else if(id == 'd201373')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1> Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A, Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 1. pSB1C3-red 7. pSB3K3-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red L. plantarum: Lb10. 256-noplasmid Lb11. 36E-noplasmid L. reuteri: Lb12. DSM 20016-noplasmid L. lactis: Lb13. MG1363-pJP059 <h2>Todays work</h2> PCR: Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* *Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Lb13.2.2 lactis MG1363 pJP059 (plasmid)** Lb13.2.3 lactis MG1363 pJP059 (plasmid)** Lb13.3 lactis MG1363 pJP059 (colony)** Lb13.4 Lc. lactis MG1363 pJP059 (colony)** **Gel will be done tomorrow. Followed the PCR protocol with DMSO. 78. pEL3C18-CrtY* -*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time) Digest: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Ligation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 1. pSB1C3-red -as N/C, one that we use plus a digest that has worked for other groups 7. pSB3K3-red -as N/C, one that we use plus a digest that has worked for other groups 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red -*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning. Transformation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77 pSB4C15-red O/N: Lb10.1. plantarum 256 no plasmid Lb11.1. plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 Plasmid preparation: 7.pSB3K3 22.pSB1C3-B0032-BFP 42. pSB4S15-red Gelelectrophoresis: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Lb13.2.3 Lc. lactis MG1363-pJP059* Lb13.3 Lc. lactis MG1363-pJP059* Lb13.4 Lc. lactis MG1363-pJP059* *Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Results Gelelectrophoresis: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red -Digestion Worked failed: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* -*The gel showed nothing but primer dimer. Digest: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Yesterdays transformation: successful: 61. pSB1C3-CP1: Growth 61. pSB1C3-CP1 10x: Some growth 62. pSB1C3-CP8: Some growth 62. pSB1C3-CP8 10x: No growth 63. pSB1C3-CP11: Some growth 63. pSB1C3-CP11 10x: No growth 64. pSB1C3-CP29: Some growth 64. pSB1C3-CP29 10x: No growth 65. pSB1C3-CP30: Some growth 65. pSB1C3-CP30 10x: No growth 66. pSB1C3-CP41: Growth 66. pSB1C3-CP41 10x: Some growth 67. pSB1C3-Cp44: No growth 67. pSB1C3-Cp44 10x: No growth 68. pSB3K3-CP1-B0032-BFP: No growth 68. pSB3K3-CP1-B0032-BFP 10x: Growth 69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated 69. pSB3K3-CP8-B0032-BFP 10x: No growth 70. pSB3K3-CP11-B0032-BFP: Growth 70. pSB3K3-CP11-B0032-BFP 10x: Growth 71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies 71. pSB3K3-CP29-B0032-BFP 10x: ----- 72. pSB3K3-CP30-B0032-BFP: Growth 72. pSB3K3-CP30-B0032-BFP 10x: No growth 73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies 73. pSB3K3-CP41-B0032-BFP 10x: No growth 77.1 pSB4C15, clone 1: No growth 77.1 pSB4C15, clone 1 10x: No growth 77.2 pSB4C15, clone 2: Some growth 77.2 pSB4C15, clone 2 10x: No growth 23. pSB1C3-CrtB 24. pSB1C3-CrtI 27. pEL3K16-red 38. pEL3A15-red 51. pEL3C18-red failed: 81. pSB4S15-CP41: No growth 82. pSB4S15-CP29: No growth 83. pSB4S15-CP30: No growth → Redo ligation and transformation. Plasmid preparation: 7.3 pSB3K3 clone 3: 50.1 ng/µl 7. 4 pSB3K3 clone 4: 43.4 ng/µl 7. 5 pSB3K3 clone 5: 48.1 ng/µl 22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl 22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ 22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ 42.1 pSB4S15-red clone 1: 43.6 ng/µl 42.2 pSB4S15-red clone 2: 46.1 ng/µl <h2>Other experiments</h2> Made agarplates with chloramphenicol resistance MRS-broth preparation MRS-broth-agar preparation MRS-broth-agar plates preparation </div>';

+

}

+

else if(id == 'd201372')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-07-02</h1> Name of participants: Christoffer Ahlström (CA), Nafisa Bashir (NB), Viktor Törnblom (VT), Anton Berglund (AB), Mikael Strandgren (MS), Viktor Blomkvist (VB), Thorsteinn O, Magnus B, Nils A, Alexander W, Christoffer F, Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli (strain D5α): 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 81. pSB4S15-CP41 82. pSB4S15-CP29 83. pSB4S15-CP30 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus L. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2> O/N: Lb9.7. faecalis JH2-2 pAMβ1 clone 7 (erythromycin) Lb9.8. faecalis JH2-2 pAMβ1 clone 8 (no-antibiotic) Lb9.9. faecalis JH2-2 pAMβ1 clone 9 (erythromycin) Lb9.10. faecalis JH2-2 pAMβ1 clone 10 (no-antibiotic) Lb10.1. plantarum 256 no plasmid Lb11.1. plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 Lb19.5 L. thermophilus Lb19.6 L. thermophilus 7.3. pSB3K3-red 7.4. pSB3K3-red 7.5. pSB3K3-red 22.1.1. pSB1C3 22.2.1. pSB1C3 22.2.2. pSB1C3 42.1.1. pSB4S15-red 42.1.2. pSB4S15-red 42.1.3. pSB4S15-red Digest: Lb9.4 faecalis JH2-2 pAMβ1 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Gel electrophoresis: Lb9.4 faecalis JH2-2 pAMβ1 Frozen stock: Lb13.3. Lc. lactis MG1363 pJP059 clone 3 Lb13.4. Lc. lactis MG1363 pJP059 clone 4 Lb15.3. Lc. lactis MG1363 pGus clone 3 Lb15.4. Lc. lactis MG1363 pGus clone 4 Lb16.3. Lc. lactis MG1363 noplasmid clone 3 Lb16.4. Lc. lactis MG1363 noplasmid clone 4 Plasmid preparation: Lb13.3. Lc. lactis MG1363 pJP059 clone 3 Lb13.4. Lc. lactis MG1363 pJP059 clone 4 Lb15.3. Lc. lactis MG1363 pGus clone 3 Lb15.4. Lc. lactis MG1363 pGus clone 4 PCR: Lb13.1 Lc. lactis MG1363 pJP059 Lb13.2 Lc. lactis MG1363 pJP059 Lb13.3 Lc. lactis MG1363 pJP059 Lb13.4 Lc. lactis MG1363 pJP059 The primers for usp45 were used in order to amplify the usp45 signal peptide from the L. lactis chromosome. A gel was run to confirm fragment size. 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL Gel electrophoresis: Lb13.1 Lc. lactis MG1363 pJP059 Lb13.2 Lc. lactis MG1363 pJP059 Lb13.3 Lc. lactis MG1363 pJP059 Lb13.4 Lc. lactis MG1363 pJP059 1. pSB1C3-red (cut with EcoRI and SpeI today) 1. pSB1C3-red (cut with EcoRI and SpeI saturday) 7. pSB3K3-red (cut with EcoRI and PstI today) 22. pSB1C3-B0032-BFP (cut with XbaI and PstI saturday) 42. pSB4S15-red (EcoRI and SpeI today) 42. pSB4S15-red (cut with SalI and SacI saturday) 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL Re-streak from frozen stock: Lb12.1 reuteri DSM 20016 no plasmid Lb12.2 reuteri DSM 20016 no plasmid Two re-streaks of each clone were made on two antibiotics free plates. Re-streak: Lb19.1 L. thermophilus Lb19.2 L. thermophilus Lb19.3 L. thermophilus Lb19.4 L. thermophilus Lb19.7 L. thermophilus Lb19.8 L. thermophilus Lb19.9 L. thermophilus Lb19.10 L. thermophilus Streaking of yogurt culture: Lb18. Lb. delbrueckii bulgaricus Lb19. Lactococcus Thermophilus Assembly and subcloning: 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77.1 pSB4C15, clone 1 77.2 pSB4C15, clone 2 had less of plasmid 42 because it ran out. 81. pSB4S15-CP41 82. pSB4S15-CP29 83. pSB4S15-CP30 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Transformation: 61. pSB1C3-CP1 * 62. pSB1C3-CP8 63. pSB1C3-CP11 * 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP * 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP * 72. pSB3K3-CP30-B0032-BFP * 73. pSB3K3-CP41-B0032-BFP * 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15, clone 1 77.2 pSB4C15, clone 2 * 81. pSB4S15-CP41 * 82. pSB4S15-CP29 83. pSB4S15-CP30 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS * Those marked were incubated for maybe 25 minutes longer. Subcloning of 57 and 58 into pSB1C3 (from 1. pSB1C3-Red). PCR of Crt Z(with 7% DMSO), 19, 20, 21(now with VF2, VR and culture) Gelelectroforesis of CrtE, CrtZ, 19, 20, 21 Transformation of 23, 24, 27, 38, 51. <h2>Results</h2> Previous day: O/N: Lb13.3 Lc. lactis MG1363 pJP059 clone 3: Good growth, NC ok! Lb13.4 Lc. lactis MG1363 pJP059 clone 4: Good growth, NC ok! Lb15.3 Lc. lactis MG1363 pGus clone 3: Good growth, NC ok! Lb15.4 Lc. lactis MG1363 pGus clone 4: Good growth, NC ok! → Continue with frozen stock and plasmid preparation. Assembly and transformation: 61. pSB1C3-CP1: Nothing 61. pSB1C3-CP1 10x: Nothing 62. pSB1C3-CP8: Growth 62. pSB1C3-CP8 10x: Nothing 63. pSB1C3-CP11: Growth 63. pSB1C3-CP11 10x: Nothing 64. pSB1C3-CP29: Growth 64. pSB1C3-CP29 10x: Nothing 65. pSB1C3-CP30: Growth 65. pSB1C3-CP30 10x: Nothing 66. pSB1C3-CP41: Nothing 66. pSB1C3-CP41 10x: Nothing 67. pSB1C3-Cp44: Growth 67. pSB1C3-Cp44 10x: Nothing 77. pSB4C15-red: Nothing 77. pSB4C15-red 10x: Nothing 45. pSB1C3-B0034-4CL Growth 46. pSB1C3-B0034-His-4CL Growth 47. pSB1C3-B0034-STS Growth 48. pSB1C3-B0034-His-STS Growth → Redo with extra care, look over the protocols, use new ligase and ligation buffer. Today: Gel electrophoresis: 43. pSB1C3-B0034-TAL Good bands 44. pSB1C3-B0034-His-TAL Good bands Lb13.1 Lc. lactis MG1363-pJP059 13.2 Lc. lactis MG1363-pJP059 13.3 Lc. lactis MG1363-pJP059 13.4 Lc. lactis MG1363-pJP059 Lb9.4 faecalis JH2-2 pAMβ1 Faint blurry bands* 1. pSB1C3-red (cut with EcoRI and SpeI today) Good bands 1. pSB1C3-red (cut with EcoRI and SpeI saturday) Good bands 7. pSB3K3-red (cut with EcoRI and PstI today) Good bands 22. pSB1C3-B0032-BFP (cut with XbaI and PstI saturday) Nothing** 42. pSB4S15-red (EcoRI and SpeI today) Nothing** 42. pSB4S15-red (cut with SalI and SacI saturday) Nothing** → 1 and 7 were ok, they could be reused. *The results were consistent with yesterdays Gel electrophoresis: Very faint smudged bands that lets us think there is only chromosomal DNA in this plasmid preparation. **The results indicate that something may be wrong with the digests of 42. and 22., this may be one of the explanations of why we got no colonies of 77. but many of 61-67. Plasmid preparation: Lb13.3. Lc. lactis MG1363 pJP059 clone 3: 13.4 ng/µl Lb13.4. Lc. lactis MG1363 pJP059 clone 4: 10.3 ng/µl Lb15.3. Lc. lactis MG1363 pGus clone 3: 11.9 ng/µl Lb15.4. Lc. lactis MG1363 pGus clone 4: 12.0 ng/µl → Dispose, to low concentration. Re-do a plasmid preparation from O/N with optimized conditions. Gel showed fail of PCR for 19,20, 21 Gel showed succes of CrtE, but fail of CrtZ(no primer dimers tho) Plates from yesterday showed fail of subclone/transformation <h2>Other experiments</h2> Made agarplates with chloramphenicol resistance Microscopy: Confirming Lc.thermophilus colonies on the yoghurt-plate from the previous day. Confirming contamination on some plates with competent cells. Primer design of lambda red, Crt Y, I and B both F and R </div>';

+

}

+

else if(id == 'd201371')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-07-01</h1> Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Christoffer, Nils, Magnus, Alexander, Anders Edlund (AE), Mikael Strandgren (MS), Christoffer Ahlström (CA), Viktor Blomkvist (VB), Viktor Törnblom(VT), Nafisa Bashir (NB), Anton Berglund (AB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 7. pSB3K3-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red 1. pSB1C3-red 60.PK401 dam- L. plantarum: Lb5. 256-rifR-pAMβ1 E. faecalis: Lb9. JH2-2 pAMβ1 L. reuteri: Lb12. DSM 20016-noplasmid L. lactis: Lb15 unknown-pGus Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus L. thermophilus: 19. L.thermophilus <h2>Todays work</h2> Gel electrophoresis: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Lb9.4 faecalis JH2-2 pAMβ1 CBP amplicon from reuteri DSM 20016 usp45 amplicon from lactis strain MG1363 PCR: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 6 colonies of DSM 20016 strain reuteri 4 colonies of MG1363 strain lactis The PCR was carried out with the CBP primers for reuteri and the usp45 primers for lactis. This in order to isolate the signal peptides from each species. O/N: Lb13. Lc. lactis MG1363 pJP059 clone 3 Lb13. Lc. lactis MG1363 pJP059 clone 4 Lb15. Lc. lactis MG1363 pGus clone 3 Lb15. Lc. lactis MG1363 pGus clone 4 7. pSB3K3-red Frozen stock: Lb5.2 plantarum 256 pAMβ1 Lb9. faecalis JH2-2 pAMβ1 clone 4 1. pSB1C3-red 60.PK401 dam Re-streak: Lb9. faecalis JH2-2 pAMβ1 Lb12. reuteri DSM20016 no plasmid Plasmid preparation: Lb5.2 plantarum 256 pAMβ1 Lb9. faecalis JH2-2 pAMβ1 clone 4 1. pSB1C3-red Digest: Lb9.4 faecalis JH2-2 pAMβ1 (To rule out chromosomal contamination in prep) Assembly: 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red Transformation: 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red* *We made a transformation with one sample of 77-digest from today, and one from yesterday to try to see if the problem yesterday laid with the assembly or with the transformation Streaking of yogurt culture: 18. Lb. delbrueckii bulgaricus 19. Lactococcus Thermophilus PCR of Crt E, Crt Z, 19, 20, 21, Idi Gelelectroforisis of Crt E, Crt Z, Idi Transformation of 75, 76, 78, 58 Subcloning of IspA ->58 Digest of Idi Ligation of 76, 78 <h2>Results</h2> Previous day: Assembly and transformation: 61. pSB1C3-CP1: No growth 62. pSB1C3-CP8: No growth 63. pSB1C3-CP11: No growth 64. pSB1C3-CP29: No growth 65. pSB1C3-CP30: No growth 66. pSB1C3-CP41: No growth 67. pSB1C3-Cp44: No growth 77. pSB4C15-red: No growth → Re-do all steps, but do one transformation with ligations from yesterday Today: Plasmid preparation: Lb5.2 plantarum 256 pAMβ1 clone 2: 50.7 ng/µl Lb9.4 faecalis JH2-2 pAMβ1 clone 4: 403.7 ng/µl 1.1 pSB1C3-red clone 1: 144.0 ng/µl 1.2 pSB1C3-red clone 2: 96.8 ng/µl Gel electrophoresis: Lb9.4 faecalis JH2-2 pAMβ1* *Very faint smudges that could be a shadow from the dye. Will follow up tomorrow with another gel electrophoresis of a new digest. CBP amplicon from reuteri DSM 20016** usp45 amplicon from lactis strain MG1363** **None of the PCRs showed the desired bands on the gel. PCR with good results: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL (Note: weak band) 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS - Very good results on the pcr Gel of Crt E and Crt Z was failed at Ethylium Bromide bath. Redo gel Gel of Idi showed succes, proceed to subclone. Subclone of IspA showed only red colonies. IspA was incorrectly digested with E instead of X. -dunno what this is! :S <h2>Other experiments</h2> M17-broth agar preparation M17-broth medium preparation SET-buffer preparation Preparation of M17 plates</div>';

+

}

+

else if(id == 'd2013721')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-07-21</h1>Name of participants:Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Viktor @, Marcus H. Nils, Christoffer, Anders Edlund (AE) '+

+

'<h2>Ongoing constructs:</h2>104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS '+

+

'99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 '+

+

'L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus '+

+

'<h2>Todays work:</h2>Plasmid Prep on: 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL 120.1 pSB1C3-B0034-HIS-4CL 104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS 106.5 and 106.6 pSB3T5-J23110-B0034-TAL 122.2 pSB1C3-B0034-His-STS; Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 Gel: 21. CrtE~I/w RBS,ZF Overnight & screening: 99. pSB1C3-CrtO 128.pSIM5-tet (E.Coli Nissle) Endast O/N 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA '+

+

'Overnight: 84.1 pSB4S15-CP6 84.2 pSB4S15-CP6 86.1 pSB4S15-CP6-B0032-BFP 86.2 pSB4S15-CP6-B0032-BFP 87(1.1) pSB4S15-CP25-B0032-BFP 87(1.2) pSB4S15-CP25-B0032-BFP 88.1 pSB4S15-CP1-B0032-BFP 88.2 pSB4S15-CP1-B0032-BFP 89.1 pSB4S15-CP44-B0032-BFP 89.2 pSB4S15-CP44-B0032-BFP 97.1 pSB4S15-CP8-B0032-BFP 97.2 pSB4S15-CP8-B0032-BFP 98.1 pSB4S15-CP11-B0032-BFP 98.2 pSB4S15-CP11-B0032-BFP '+

+

'<h2>Results:</h2> Re-streak: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked Gel 21. CrtE~I/w RBS,ZF - fail Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA - Fail of all clones 109. pSB4A15-K206000-B0034-IspA - Fail of all clones 110. pSB4A15-K206001-B0034-IspA - Fail of all clones </div>';

+

}

+

else if(id == 'd2013720')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1>Name of participants: Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH) <h2>Ongoing constructs:</h2>99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Re-streak: 84. pSB4S15-CP6 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 99. pSB1C3-CrtO Overnigth: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 111.6 pSBLb4C15-red 111.7 pSBLb4C15-red Transformation: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 134. pSB1C3-CBP 135. pSB1C3-CSP 136. pSB1C3-USP45 Plasmidprep: 109. pSB4A15-K206000-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 1.1? 90.3 j23101 1.2? 2.1?90.4 j23101 2.2? Gel: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening pcr of constructs: 119.pSB1C3-B0034-4CL 120 pSB1C3-B0034-HIS-4CL 106 pSB3T5-J23110-B0034-TAL 117 pSB3T5-CP1-B0034-His-TAL 107 pSB3T5-CP1-B0034-TAL PCR: 21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor) 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA <h2>Results</h2>Overnight & screening: 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA Screening of: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.Gel Gel succes on: 106.5 pSB3T5-J23110-B0034-TAL 106.6 pSB3T5-J23110-B0034-TAL 117.1 pSB3T5-CP1-B0034-His-TAL 117.2 pSB3T5-CP1-B0034-His-TAL 119.2 pSB1C3-B0034-4CL No band on: 107 pSB3T5-CP1-B0034-TAL 116 pSB3T5-J23110-B0034-His-TAL 120 pSB1C3-B0034-HIS-4CL </div>';

+

}

+

else if(id == 'd2013719')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1> Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, Christoffer Ahlström (CA), Stephanie Herman (SH) <h2>Ongoing constructs:</h2>E.coli (strain D5α): 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 136.1 pSB1C3-usp45 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</h2>Screening pcr of constructs: 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 117. pSB3T5-CP1-B0034-His-Tal 119. pSB1C3-B0034-4CL (from 15) 120. pSB1C3-B0034-His-4CL (from 15) Overnight & screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Plasmid preparation: 122.2. pSB1C3-B0034-His-STS (from 15) 90.3. BBa_J61002 (amp)-J23101 90.4. BBa_J61002 (amp)-J23101 104.2. pSB1C3-B0034-4CL-B0034-STS 104.5. pSB1C3-B0034-4CL-B0034-STS 111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15 121.1. pSB1C3-B0034-STS (from 15) 121.2. pSB1C3-B0034-STS (from 15) Cloning: 21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red Assembly: 21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red 21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <Gel electrophoresis: 129. pSB1C3-Idi (mut) Restreak 128. pSIM5-tet(Nissle) <h2>Results:</h2>Screening: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight. showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight. The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2. Transformation: Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45. → Re-do the transformation with the saved ligation solution from (18/07-13) → Re-streak the possible white colonies. </div>';

+

}

+

else if(id == 'd2013718')

+

{

+

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB) <h2>Ongoing constructs:</h2>E.coli (strain D5α): 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 90. BBa_J61002-J23101 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Gel-electrophoresis of screening PCR products 42. pSB4S15-red Digestion: 42. pSB4S15-red Transformation: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Overnight: 90. BBa_J61002-J23101 Spreading of strains: 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Re-circulization: 129. pSB1C3-Idi (mut) Plasmid Prep (Glycerol stock): 75. pEL3A15 - CrtB (75.1, 75.2, 75.4) Transformation: 99. pSB1C3 - Crto 129. pSB1C3-Idi (mut) 113. E.coli Nissle-pSIM5-tet 111. pSBLb4C15 112. pSBLbEc4C15 Re-streak: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His Ligation of strains from yesterdays digest: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 *Ligated to 1.1 pSB1C3-red (From strain database 2013) Transformation:* Collagen binding protein Cell surface protein usp45 *Performed with D5(alpha) competent cells. Screening: 111. pSBLb4C15 Spreading of strains: Collagen binding protein [D5(alpha)] * Cell surface protein [D5(alpha)] * usp45 [D5(alpha)] * 111. pSBLb4C15 ** 112. pSBLbEc4C15 ** *With a 10x and 1x dilution. ** 2 plates of each with 1x dilution. <h2>Results</h2>Re-streaks: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His were successful on all clones but 108.7. Transformation of 129. pSB1C3-Idi (mut) did not grow. Electrophoresis on digests: *The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. </div>';

+

}

+

else if(id == 'd2013717')

+

{

+

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-18</h1> Name of participants: Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</h2>E.coli (strain D5α): 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 57.Psb1C3-B0034-Idi 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. Lc.thermophilus <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Ligation 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-His-4CL Digest & Ligation: ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2 B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His Digest Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 1.1 pSB1C3-red (From strain database 2013) Transformation: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His O/N & Screening: 75. pEL3A15 - CrtB 102. pSB1C3 - CrtE Plasmid Prep(Glycerol stock) 79. pEL3K16 - CrtE 101. pSB1C3 - DNA program123456 PCR 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor) 57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. Gel-electrophoresis of screening PCR products: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Sequencing preparation: 62. pSB1C3-CP8 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Re-streak: Lb18. Lb. delbrueckii bulgaricus no plasmid Spreading of strains: Lb19. Lc.thermophilus no plasmid* *With 1x, 10x & 50x dilution <h2>Results</h2>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers. 101. pSB1C3 - DNA program123456 worked and sent for sequence Transformations: 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 123. pSB1C3-ZCD 125. pSB1C3-ZCD-His 126. pSB1C3-UGTCs2 127. pSB1A3-B0034-UGTCs2-His all grew red and white colonies, indicating successful ligation and transformation. Gel-electrophoresis of overnight PCR products: Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp. Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp. usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp. </div>';

+

}

+

else if(id == 'd2013716')

+

{

+

ds = '<div id="dairy-text"> <h1>Tuesday 2013-07-16</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 75. pEL3A15 - CrtB 99. pSB1C3 - CrtO 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 <h2>Todays work</h2>Assembly 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL Ligation: 48. pSB1C3-B0034-His-STS Gel on Digest: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS Restreak: 47. pSB1C3-B0034-STS 75. pEL3A15 - CrtB Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP 27. pEL3K16-red Overnight: 74. pSB3K3-CP44-B0032-BFP Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 Transformation: 99. pSB1C3 - CrtO O/N & Screening 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 101. pSB1C3 - DNA program123456 Gelelectrophoresis 21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF 57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) PCR 57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <h2>Results</h2>Plasmid preparation: 74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do! Screening: 111. pSBLb4C15: Failed 112. pSBLbEc4C15: Failed <h2>Other experiments</h2>Primer stock preparation: CA * CSP_RF CBP_RF usp45_RF CBP F *Obtained a concentration of 5µM at a volume of 200µl. </div>';

+

}

+

else if(id == 'd2013715')

+

{

+

ds = '<div id="dairy-text"> <h1>Monday 2013-07-15</h1> Name of participants: Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas <h2>Ongoing constructs:</h2>E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 105. pSB3T5-J23101-B0034-TAL 104. pSB1C3-B0034-4CL-B0034-STS 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB 101. pSB1C3 - DNA program123456 76. pEL3K16-CrtI 78. pEL3C18-CrtY 99. pSB1C3 - CrtO 57.Psb1C3-B0034-Idi 91. BBa_J61002 (amp)-J23106 94. pSB1C3-K206000 95. pSB1C3-K206001 11. pSB4A15-blue(?) 58. pSB1C3-IspA 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) 22. Betacarotene-various plasmids L. plantarum: 5. 256-rifR-pAMβ1 10. 256-p256 <h2>Todays work</h2>PCR Purification: 111. pSBLb4C15, digest 112. pSBLbEc4C15, digest Ligation: 111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) 112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI) Transformation: 111. pSBLb4C15 112. pSBLbEc4C15 75. pEL3A15 - CrtB 99. pSB1c3 - CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Overnight: 74. pSB3K3-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Plasmid preparation: Lb10.1 plantarum 256 p256 Lb10.2 plantarum 256 p256 Lb10.3 plantarum 256 p256 Lb22.1 reuteri betacarotene (strain name?) various plasmids 44.5 pSB1C3-b0034-His-Tal 74. pSB3K3-CP44-B0032-BFP PCR: 48. pSB1C3-B0034-His-STS Gel electhrophoresis 48. pSB1C3-B0034-His-STS Assembly: 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL Re-streak: 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB PCR cloning of: CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger Mutagenesis 57. pSB1C3-B0034-Idi Sequence preperation 78.1 pEL3C18-CrtY (VF and VR) 78.3 pEL3C18-CrtY (VF and VR) Screening: 111. pSBLb4C15 112. pSBLbEc4C15 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 101. pSB1C3 - DNA program123456 Touchdown PCR with Phusion polymerase (signal peptides): CA Collagen binding protein → cloned from strain 14.4 Cell surface protein → cloned from strain 3.1 usp45 → cloned from strain 13.1 <h2>Results</h2>PCR, Succes on: 48. pSB1C3-B0034-His-STS Transformation 45. pSB1C3-B0034-4CL, failed 46. pSB1C3-B0034-His-4CL, failed 104. pSB1C3-B0034-4CL-B0034-STS, failed 106. pSB3T5-J23110-B0034-TAL, failed 108. pSB4A15-J23106-B0034-IspA, failed 109. pSB4A15-K206000-B0034-IspA, failed 110. pSB4A15-K206001-B0034-IspA, failed 111. pSBLb4C15 112. pSBLbEc4C15 -(45,46,104,106) Probably due to weak competent cells -(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests. Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter. Gel: 21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail Fluorescence(BFP) experiment with UV: 68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4) 69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4) 70. pSB3K3-CP11-B0032-BFP : Glowing (70.3) 71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2) 72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2) 73. pSB3K3-CP41-B0032-BFP : Glowing (73.4) 74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2) 81. pSB4S15-CP41-B0032-BFP : Not glowing 83. pSB4S15-CP30-B0032-BFP : Not glowing 86. pSB4S15-CP6-B0032-BFP : Not glowing 88. pSB4S15-CP1-B0032-BFP : Not glowing 89. pSB4S15-CP44-B0032-BFP : Not glowing Plasmid preparation: Lb10.1 plantarum 256 p256: 124.7 ng/µl Lb10.2 plantarum 256 p256: 23.5 ng/µl Lb10.3 plantarum 256 p256: 270.9 ng/µl Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl </div>';

+

}

+

else if(id == 'd2013714')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-07-14</h1>Name of participants: Anders Edlund (AE), Viktor Blomkvist (VB), Lovisa P, Christoffer F, Sabri J <h2>Ongoing constructs:</h2>E.coli (strain D5α): 7.pSB3k3-red 22. B0032-BFP 42. pSB4S15-red 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 111. pSBLb4C15 112. pSBLbEc4C15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 91. BBa_J61002-J23106 92. BBa_J61002-J23110 93. BBa_J61002-J23116 103. pSB1k3-J23101 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB 101. pSB1C3 - DNA program123456 76. pEL3K16-CrtI 78. pEL3C18-CrtY L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 14. DSM 20016-pLUL631(B?) 22. Betacarotene-various plasmids L. plantarum: 5. 256-rifR-pAMβ1 10. 256-p256 <h2>Todays work</h2>Ligation: 111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) 112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI) Transformation: 111. pSBLb4C15 112. pSBLbEc4C15 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 75. pEL3A15 - CrtB Plasmid prep. and glycerol stock: 103. pSB1k3-J23101 91. BBa_J61002-J23106 92. BBa_J61002-J23110 93. BBa_J61002-J23116 44.4 pSB1C3-b0034-His-Tal 76.(1-4)-pEL3K16-CrtI 78.(1-3)-pEL3C18-CrtY Overnight: 44.5 pSB1C3-b0034-His-Tal BBa_J61002-J23101 (amp) Restreak: 101. pSB1C3 - DNA program123456 (Picked 4 clones) Subcloning: CrtE (PCR prod) should be digested with X,P (Can be found in digest box) 27. pEL3K16 - red should be digested with X,P → CrtE (PCR prod) 1. pSB1C3 - red should be digested with X,P → CrtE (PCR prod) 38. pEL3A15 - red → 23. pSB1C3-CrtB PCR cloning: CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger CrtO PCR is done to clone CrtO from the following template, digested (E,P) 19. pBAD/AraC-CrtE~Crt(O)-Crt(Z) /w RBS, Zinc finger <h2>Results</h2>Problems with the yesterday ligation, the experiment was redone Transformation of 101. DNA program123456 was a sucess although 102. pSB1C3-CrtE failed The PCR failed for CrtE~I, unspecific binding The PCR failed for CrtZ, unspecific binding. </div>';

+

}

+

else if(id == 'd2013713')

+

{

+

ds = '<div id="dairy-text"> <h1>Saturday 2013-07-13</h1>Name of participants: Sabri J, Nils, Kristoffer L, Theodor Löwe, Viktor Blomkvist (VB), Stephanie Herman (SH), Alona Nyberg (AN) <h2>Ongoing constructs:</h2>E.coli (strain D5α): 101. pSB1C3 - DNA Program123456 79. pEL3K16 - CrtE 102. pSB1C3 - CrtE 76. pEL3K16 - CrtI 78. pEL3C18 - CrtY 90 pSB1k3-J23101 91 BBa_J61002-J23106 92 BBa_J61002-J23110 93 BBa_J61002-J23116 44.4 pSB1C3-b0034-His-Tal 44.5 pSB1C3-b0034-His-Tal 111. pSBLb4C15: MluI+NheI 112. pSBLbEc4C15: MluI+ClaI 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP <h2>Todays work</h2>Ligation: 1. pSB1C3 - red → DNA program123456 (PCR prod) 1. pSB1C3 - red → CrtE (PCR prod) 27. pEL3K16 - red → CrtE (PCR prod) PCR and Gelelectrophoresis: CrtO Transformation: 101. pSB1C3 - DNA program123456 102. pSB1C3 - CrtE Overnight, Screening: 76.(1-4) pEL3K16 - CrtI 78.(1-3) pEL3C18 - CrtY PCR purification: 111. pSBLb4C15 112. pSBLbEc4C15 Digestion: 1. pSB1C3 - red → DNA program123456 (PCR prod) 111. pSBLb4C15: MluI+NheI 112. pSBLbEc4C15: MluI+ClaI Lb13. Lc. lactis MG1363-pJP059, Ori: Two experiments, Mlu1+NheI & MLuI+ClaI * Parts of what will become the first shuttle-vector. Plasmid preparation: 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP <h2>Results</h2>Gel: 47. pSB1C3-B0034-STS PCR product with and without DMSO (7%) Good results on GEL, both PCR with and without DMSO looks good. <img class="result-pic" src="https://static.igem.org/mediawiki/igem.org/4/43/Uppsala_gelPic_2013-07-13.jpg"><table class="table-pic-desc-table"><tr><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td><td>9</td><td>10</td></tr><tr><td>N/C</td><td>76.1 (Sucess)</td><td>76.2 (Sucess)</td><td>76.3 (Sucess)</td><td>76.4 (Sucess)</td><td>Ladder</td><td>N/C</td><td>78.1 (Sucess)</td><td>78.2 (Sucess)</td><td>78.3 (Sucess)</td></tr><tr><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td></tr><tr><td>Ladder</td><td>CrtE (digested with E,P)</td><td>CrtE~I (digested with X, P) (Failed)</td><td>CrtE~I (digested with E, P) (Failed)</td><td>N/C</td><td>CrtO (Failed)</td><td>ORI I (Geneticals)</td><td>ORI II (Geneticals)</td><td>Ladder</td></tr></table> CrtE PCR prod should have been digested with X,P therefore construct 79 and 102 needs to be redone </div>';

+

}

+

else if(id == 'd2013712')

+

{

+

ds = '<div id="dairy-text"> <h1>Friday 2013-07-12</h1>Name of participants: Kristoffer L, Emil M, Karl H, Peter C, Lovisa P, Theodor L, Ken B-A, Christoffer F, Sabri J, Niclas <h2>Ongoing constructs:</h2>E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 82. pSB4S15-CP29-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 87. pSB4S15-CP25-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus Lc. thermophilus: 19. L.thermophilus L. plantarum: 5. 256-rifR-pAMβ1 10. 256-p256 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid <h2>Todays work</h2>Digestion: 22. B0032-BFP - E,P 11. psB4A15 Ligation: CrtE~I /w RBS + Zincfinger -> 11. pSB4A15-ed CrtE(PCR-prod.) -> 27. pEL3K16-red CrtE -> 1. pSB1C3 (to registry) -all AMP plates from yesterday failed 90-93. (J23 series promotors) Digest of construct 14 and 15 from plasmid prep with Pst1, followed by gel analysis. Transformation: CrtE~I /w RBS + Zincfinger -> 11. pSB4A15-ed CrtE(PCR-prod.) -> 27. pEL3K16-red 82. pSB4S15-CP29-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 87. pSB4S15-CP25-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 100. pJP059 J23101 PCR on DNA program 123456 Plasmid prepp on 96.Cs42s clone nr 1 and 2. Glycerol stock on 96.Cs42s clone 1 and 2. Gel run on digest from plasmid preparation of 96.cs42s clone 1 and 2. Re-streak: 76 pEL3K16 - CrtI (4 clones) 78. pEL3C18 - CrtY (3 clones) 74. pSB3K3-CP44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP Gel electrophoresis: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 46. pSB1C3-B0034-His-4CL 48. pSB1C3-B0034-His-STS pSB4C15 PCR-product with intact ori pSB4C15 PCR-product without ori Overnight culture: 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP Lb10.3 plantarum 256 p256 Lb10.4 plantarum 256 p256 Spreading: 87. pSB4S15-CP25-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Lb10.3 plantarum 256 p256 Lb10.4 plantarum 256 p256 <h2>Results</h2>Transformation: Successful: 76 pEL3K16-CrtI 78 pEL3C18-CrtY failed: 75 pEL3A15-CrtB The result from nanodrop measurement of 96.Cs42s clone 1 was 408.3 nanogram/microliter and clone 2 was 332. Also both had a god a260/a280 ratio. The result from the gel run of the two 96.Cs42s clones indicates an construct with a size of ~4000 bp. Gel on PCRs: Successful: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL failed 47. pSB1C3-B0034-STS 46. pSB1C3-B0034-His-4CL 48. pSB1C3-B0034-His-STS -PCR-amplification: 47. pSB1C3-B0034-STS, change temperature -2 degrees Construct 14 had two bands on the gel, indicating illegal restriction sites. Construct 15 only had one band and no indication of illegal restriction sites. <h2>Other experiments</h2>Casting LB-agar plates: AB 27 plates containing spectinomycin. Preparation for sequencing: 69.1 pSB3K3-CP8-B0032-BFP 71.3 pSB3K3-CP29-B0032-BFP 72.3 pSB3K3-CP30-B0032-BFP Dilution of primers: 200 µl VF2 (5 µM) 200 µl VR (5 µM) </div>';

+

}

+

else if(id == 'd2013711')

+

{

+

ds = '<div id="dairy-text"> <h1>Thursday 2013-07-11</h1> Name of participants: Kristoffer L, Emil M, Karl H, Peter C, Lovisa P, Theodor L, Ken B-A, Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Alona Nyberg (AN), Viktor Törnblom (VT), Viktor Blomkvist (VB), Mikael Strandgren (MS), Niclas, Sabri , Nils, Christoffer <h2>Ongoing constructs:</h2> E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 84. pSB4S15-CP6 85. pSB4S15-CP25 96. Cs42s L. plantarum: 10. 256-p256 <h2>Todays work</h2> Transformation: 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 77. pSB4C15-red* 75. pEL3A15 - CrtB 76 pEL3K16 - CrtI 78. pEL3C18 - CrtY Restreaks: 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP Lb10.3 plantarum 256 p256 * Lb10.4 plantarum 256 p256 Lb10.5 plantarum 256 p256 * Lb10.6 plantarum 256 p256 PCR: 77.(1-4) pSB4C15-red* *Two different amplifications of plasmid adding restriction-sites with different primers. One product with no removals and one without ori. PCR-amplification: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Digest: 14 pET13b(amp)-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT -from plasmid prep with Pst1. PCR screening & gel extraction: 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL Ligation: 84. pSB4S15-CP6 85. pSB4S15-CP25 Plasmid preparation: 77. pSB4C15-red PCR screening of cs42s with RedTaq. Overnight culture on transfrormed 96.Cs42s. Prepare of Vf2 and VR primer solution, 20x. 5 tubes each with 200 microliter/tube. Subcloning of the following CrtB, CrtI, CrtY 23. pSB1C3-CrtB → pEL3A15 - red 24. pSB1C3-CrtI → pEL3K16 - red 25. CrtY(PCR prod.) → pEL3C18 - red <h2>Results</h2> Plasmid preparation: 77.1 pSB4C15-red: 47.2 ng/µl 77.2 pSB4C15-red: 50.2 ng/µl 77.3 pSB4C15-red: 44.0 ng/µl 77.4 pSB4C15-red: 54.4 ng/µl Screening: didn’t look good, the pcr product was approximatley 1000 bp on the gel. Should have been around 4000. <h2>Other experiments</h2> Sequence verification: 61.1 pSB1C3-CP1 63.1 pSB1C3-CP11 67.1 pSB1C3-Cp44 Casting LB-agar plates: 26 plates containing spectinomycin. Preparation of LB-agar solution: transformation from kit: 90. BBa_J61002 (amp)-J23101 91. BBa_J61002 (amp)-J23106 92. BBa_J61002 (amp)-J23110 93. BBa_J61002 (amp)-J23116 Restreek from stock: 2012 SD(Strain Database) 13 and 61(promotors). Aliquoting VF2- and VR-primers. </div>';

+

}

+

else if(id == 'd2013710')

+

{

+

ds = '<div id="dairy-text"> <h1>Wednesday 2013-07-10</h1> Name of participants: Kristoffer L, Emil M, Karl H, Peter C., Lovisa P, Ken B-A, Alexander, Niclas, Magnus, Nils, Thorsteinn, Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Viktor Törnblom (VT) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 96. p?A?-Cs42s 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB3K3-CP44-B0032-BFP 87. pSB3K3-CP1-B0032-BFP 88. pSB3K3-CP41-B0032-BFP 89. pSB4S15-CP44-B0032-BFP <h2>Todays work</h2> Digestion: 22. pSB1C3-RBS-B0032-BFP Ligation: 44. pSB1C3-B0034-His-TAL 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB3K3-CP44-B0032-BFP 87. pSB3K3-CP1-B0032-BFP 88. pSB3K3-CP41-B0032-BFP 89. pSB4S15-CP44-B0032-BFP transformation: 44. pSB1C3-B0034-His-TAL 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 86. pSB3K3-CP44-B0032-BFP 87. pSB3K3-CP1-B0032-BFP 88. pSB3K3-CP41-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Re-streak 96. p?A?-cs42s -Streak of transformations of ligated 20 and positive controll (AV,NA) PCR of 19(digested EP), 20(digested EP), 21(digested EP) and CrtO(from 21digestEP) (AV,NA) Spreading of strains: Lb4. reuteri 100-23 no plasmid Lb12. reuteri DSM 20016 no plasmid Lb10. plantarum 256 p256 Lb11. plantarum 36E no plasmid Lb10. plantarum 256 noplasmid: Growth Lb11. plantarum 36E noplasmid: Growth → Spread out (1x) on erythromycin plates, to examine whether they are naturally resistant. <h2>Results</h2> Transformation: Lb4. reuteri 100-23 noplasmid: Growth Lb12. reuteri DSM 20016 noplasmid: Growth Re-streak: 96. p?A?-cs42s worked well. O/N culture: 69.1 pSB3K3-CP8-B0032-BFP 69.2 pSB3K3-CP8-B0032-BFP 69.3 pSB3K3-CP8-B0032-BFP 69.4 pSB3K3-CP8-B0032-BFP 70.1 pSB3K3-CP11-B0032-BFP 70.2 pSB3K3-CP11-B0032-BFP 70.3 pSB3K3-CP11-B0032-BFP 70.4 pSB3K3-CP11-B0032-BFP 71.1 pSB3K3-CP29-B0032-BFP 71.2 pSB3K3-CP29-B0032-BFP 71.3 pSB3K3-CP29-B0032-BFP 71.4 pSB3K3-CP29-B0032-BFP 72.1 pSB3K3-CP30-B0032-BFP 72.2 pSB3K3-CP30-B0032-BFP 72.3 pSB3K3-CP30-B0032-BFP 72.4 pSB3K3-CP30-B0032-BFP *Growth Plasmid preparation: 69.1 pSB3K3-CP8-B0032-BFP: 80.5 ng/µl 69.2 pSB3K3-CP8-B0032-BFP: 75.7 ng/µl 69.3 pSB3K3-CP8-B0032-BFP: 60.5 ng/µl 69.4 pSB3K3-CP8-B0032-BFP: 50.8 ng/µl 70.1 pSB3K3-CP11-B0032-BFP: 71.7 ng/µl 70.2 pSB3K3-CP11-B0032-BFP: 56.3 ng/µl 70.3 pSB3K3-CP11-B0032-BFP: 56.0 ng/µl 70.4 pSB3K3-CP11-B0032-BFP: 45.3 ng/µl 71.1 pSB3K3-CP29-B0032-BFP: 54.0 ng/µl 71.2 pSB3K3-CP29-B0032-BFP: 49.1 ng/µl 71.3 pSB3K3-CP29-B0032-BFP: 83.0 ng/µl 71.4 pSB3K3-CP29-B0032-BFP 67.8 ng/µl 72.1 pSB3K3-CP30-B0032-BFP 53.8 ng/µl 72.2 pSB3K3-CP30-B0032-BFP 71.8 ng/µl 72.3 pSB3K3-CP30-B0032-BFP 109.6 ng/µl 72.4 pSB3K3-CP30-B0032-BFP 106.8 ng/µl </div>';

+

}

+

else if(id == 'd201379')

+

{

+

ds = '<div id="dairy-text"> <h1>Tuesday 2013-07-09</h1> Name of participants: Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Lovisa P, Theodor L, Nils, Alexander, Christoffer, Niclas, Magnus, Thorsteinn, Christoffer Ahlström (CA), Stephanie Herman (SH), Nafisa Bashir (NB), Anton Berglund (AB), Mikael Strandgren (MS), Viktor Blomkvist (VB), Viktor Törnblom (VT) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 96. p?-Cs42s 81. pSB4S15-CP41 83. pSB4S15-CP30 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB3K3-CP44 87. pSB3K3-CP1 88. pSB3K3-CP41 89. pSB4S15-CP44 90. pSB4S15-CP1 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP L. reuteri: Lb4. 100-23-no plasmid Lb12. DSM 20016-no plasmid Lb10. 256-noplasmid LB11. 36E-noplasmid <h2>Todays work</h2> Gel analysis: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL Ligation: 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL OD600 measurement: Lb4. reuteri 100-23 no plasmid Lb12. reuteri DSM 20016 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Competent cells preparation: Lb4. reuteri 100-23 no plasmid Lb12. reuteri DSM 20016 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Transfromation: Lb4. reuteri 100-23 no plasmid Lb12. reuteri DSM 20016 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Re-streaks: 77. pSB4C15-red Transformation: 81. pSB4S15-CP41 83. pSB4S15-CP30 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB3K3-CP44 87. pSB3K3-CP1 88. pSB3K3-CP41 89. pSB4S15-CP44 90. pSB4S15-CP1 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL O/N culture: 69.1 pSB3K3-CP8-B0032-BFP 69.2 pSB3K3-CP8-B0032-BFP 69.3 pSB3K3-CP8-B0032-BFP 69.4 pSB3K3-CP8-B0032-BFP 70.1 pSB3K3-CP11-B0032-BFP 70.2 pSB3K3-CP11-B0032-BFP 70.3 pSB3K3-CP11-B0032-BFP 70.4 pSB3K3-CP11-B0032-BFP 71.1 pSB3K3-CP29-B0032-BFP 71.2 pSB3K3-CP29-B0032-BFP 71.3 pSB3K3-CP29-B0032-BFP 71.4 pSB3K3-CP29-B0032-BFP 72.1 pSB3K3-CP30-B0032-BFP 72.2 pSB3K3-CP30-B0032-BFP 72.3 pSB3K3-CP30-B0032-BFP 72.4 pSB3K3-CP30-B0032-BFP Elution and transformation of 96. The transfrormed samples were plated (80μl of 1x and 200μl of 10x dilution) on all available resistances, as the backbone was unknown. Ligation of 20 with all diffrent combinations of ligase buffer and ligase. (AV, CF, NA) Transformation of ligation mentioned above, and a positive controll of 20 plasmidprepp. Although plates for streaking were missing so we couldn’t streak (CF, AV, NA) <h2>Results</h2> O/N: Lb4. reuteri 100-23 no plasmid (clone 1): NC ok! → follow protocol for transformation Lb12. reuteri DSM 20016 no plasmid (clone 1): NC ok! → follow protocol for transformation, watch in microscope for contamination Lb10. plantarum 256 no plasmid (clone 1): NC ok! → follow protocol for transformation Lb11. plantarum 36E no plasmid (clone 1): NC ok! → follow protocol for transformation Transformation: 77. pSB4C15-red: Growth on Cm, some red colonies, some blue, many white (?!) OD600 measurement: Lb4. reuteri 100-23 no plasmid: 0.195 (Measure) & 0.668 (Cultivate) → proceed Lb12. reuteri DSM 20016 no plasmid: 0.179 (Measure) & 0.680 (Cultivate) --”-- Lb10. plantarum 256 no plasmid: 0.115 (Measure) & 0.724 (Cultivate) --”-- Lb11. plantarum 36E no plasmid: 0.148 (Measure) & 0.624 (Cultivate) --”-- Microscopy: Lb12. reuteri DSM 20016 no plasmid: No contamination was sighted! → proceed! transformation: Successful: 45. pSB1C3-B0034-4CL 96. p?-Cs42s failed: 44. pSB1C3-B0034-His-TAL <h2>Other experiments</h2>Cold buffer preparation (1 l) 50% glycerol stock preparation (100 ml) Microscopy: Lb12. reuteri DSM 20016 no plasmid </div>';

+

}

+

else if(id == 'd201378')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-07-08</h1> Name of participants: Alexander, Nils, Christofffferz , Magnus, Niclas, Thorsteinn, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Lovisa P <h2>Ongoing constructs</h2> E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0044-IspA 81. pSB4S15-CP41 83. pSB4S15-CP30 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB3K3-CP44 87. pSB3K3-CP1 88. pSB3K3-CP41 89. pSB4S15-CP44 90. pSB4S15-CP1 L. reuteri: Lb4. 100-23-no plasmid Lb12. DSM 20016-no plasmid L. plantarum: Lb10. 256-noplasmid LB11. 36E-noplasmid <h2>Todays work</h2> PCR amplification and gel analysis: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL Plasmid prep.: 47. pSB1C3-B0034-STS Clone: 47.9 and 47.20 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT Sequencing prep.: 47. pSB1C3-B0034-STS Clone: 47.9 and 47.20 48. pSB1C3-B0034-His-STS Clone: 48.1 Gel extraction: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL Restreak: 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP Digest PCR products from succefull gel extraction. Ligation: 81. pSB4S15-CP41 83. pSB4S15-CP30 84. pSB4S15-CP6 85. pSB4S15-CP25 86. pSB3K3-CP44 87. pSB3K3-CP1 88. pSB3K3-CP41 89. pSB4S15-CP44 90. pSB4S15-CP1 Frozen stock: Lb4. reuteri 100-23 no plasmid (clone 1 & 2) Lb12. reuteri DSM 20016 no plasmid (clone 1 & 2) Lb10. plantarum 256 noplasmid (clone 1 & 2) Lb11. plantarum 36E no plasmid (clone 1 & 2) O/N: Lb4. reuteri 100-23 no plasmid (clone 1) Lb12. reuteri DSM 20016 no plasmid (clone 1) Lb10. plantarum 256 no plasmid (clone 1) Lb11. plantarum 36E no plasmid (clone 1) Screening: 43. pSB1C3-B0034-TAL O/N culture: 43.7. pSB1C3-B0034-TAL Subcloning: 23->4, 24->4, 25->4(kontroll), 23->38, 24->27, 25->51 (CF, AV) Transformation of 23->4, 24->4, 25->4(kontroll), 23->38, 24->27, 25->51 (CF, AV) Primer design and order of lambda red (NA) Sequencing preparation of 57.3 and 57.5 (pSB1C3-B0034-Idi), 58.1 and 58.4 (pSB1C3-B0034-IspA) <h2>Results</h2> Microscopy: * *No contamination was seen, save plates for later. → Proceed with O/N of two clones from the no-plasmid strains. Primers for lambda red and Cs42s were ordered today :D. Transformation of 23->4(blank), 24->4(blank), 25->4(blank), 23->38(blank), 24->27(a few clones, both red and white on x1), 25->51(blank) PCR amplification: 43-46 failed, most likely primer dimers - retry with different concentrations of template DNA, higher concentration of DMSO and a colony PCR of each construct. gel extraction: Successfull 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL failed 43. pSB1C3-B0034-STS 46. pSB1C3-B0034-His-4CL Screening: one succefull clone - 43.7 pSB1C3-B0034-STS <h2>Other experiments</h2> Microscopy: CA* *Of plates from 04/07 and O/N from 07/07 to see if contaminated. </div>';

+

}

+

else if(id == 'd201377')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-07-07</h1> Name of participants:Anders Edlund (AE) <h2>Ongoing constructs</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 77. pSB4S15-C-resistance <h2>Todays work</h2> Transformation (AE) 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 77. pSB4S15-C-resistance </div>';

+

}

+

else if(id == 'd201376')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-07-06</h1> Name of participants: Stephanie Herman (SH), Anders Edlund (AE), Nafisa Bashir (NB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Todays work</h2> Plasmid Preparation: 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 Transformation: 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 <h2>Results</h2> Previous day: The O/N on the nonplasmids 4, 10, 11, 12 from the previous day had grown at a previously unexperienced rate. A contamination of the original plates from Stefan Roos was suspected as the NC was clear. The O/N was put in the cold room awaiting examination by microscope. Today: Plasmid Preparation: 42. pSB4S15-red 61. pSB1C3-CP1: (1) 203.2, (2) 202.9 62. pSB1C3-CP8: (1) 254.5, (2) 229.2 63. pSB1C3-CP11: (1) 165.0, (2) 260.1 64. pSB1C3-CP29: (1) 315.4, (2) 221.9 65. pSB1C3-CP30: (1) 243.1, (2) 101.5 66. pSB1C3-CP41: (1) 190.7, (2) 68.4 67. pSB1C3-Cp44: (1) 169.1, (2) 188.7 </div>';

+

}

+

else if(id == 'd201375')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1> Name of participants: Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer <h2>Ongoing constructs:</h2> 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-CP44 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA L. reuteri Lb4. 100-23 no plasmid Lb12. DSM 20016 no plasmid L. plantarum: Lb10. 256 no plasmid Lb11. 36E no plasmid L. thermophilus: Lb19. L.thermophilus Todays work Screening PCR & gel analysis of constructs: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 Plasmid prep & glycerol stock: 48. pSB1C3-B0034-His-STS O/N: 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 47.(9, 20) pSB1C3-B0034-STS Lb4. reuteri 100-23 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Lb12. reuteri DSM 20016 no plasmid Re-streaks: Lb19. L.thermophilus <h2>Results</h2> The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB. PCR screen and gel electrophoresis: 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 *The gel only showed smudgy bands. succesful 47.9 pSB1C3-B0034-STS 47.20 pSB1C3-B0034-STS failed 43. pSB1C3-B0034-TAL 46. pSB1C3-B0034-His-4CL No contamination in negative control -_- Religation from yesterday failed. No colonies, only a few at 76, both red and white. <h2>ther experiments</h2> Contamination control: CA * *Contamination control of growth medium for Lc.lactis and Lb.reuteri. Two test from both bottles. E.coli growth test: CA * *To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </div>';

+

}

+

else if(id == 'd201374')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-07-04</h1>Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Nafisa Bashir (NB), Mikael Strandgren (MS), Anton Berglund (AB), Magnus, Thorsteinn, Alexander, Nils, Christoffer <h2>Ongoing constructs</h2>E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 42. pSB4S15-red 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-CP44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 72. pSB3K3-CP30-B0032-BFP L. lactis: Lb13. MG1363-pJP059 Lb15. unknown-pGus E. faecalis: Lb9. JH2-2 pAMβ1 <h2>Todays work</h2>Frozen stock and plasmid preparation: Lb9.7. faecalis JH2-2 pAMβ1 clone 7 (erythromycin) Lb9.8. faecalis JH2-2 pAMβ1 clone 8 (no-antibiotic) Lb9.9. faecalis JH2-2 pAMβ1 clone 9 (erythromycin) Lb9.10. faecalis JH2-2 pAMβ1 clone 10 (no-antibiotic) Lb13.5. Lc. lactis MG1363 pJP059 clone 5 Lb13.6. Lc. lactis MG1363 pJP059 clone 6 Lb13.7. Lc. lactis MG1363 pJP059 clone 7 Lb13.8. Lc. lactis MG1363 pJP059 clone 8 Lb15.5. Lc. lactis MG1363 pGus clone 5 Lb15.6. Lc. lactis MG1363 pGus clone 6 Lb15.7. Lc. lactis MG1363 pGus clone 7 Lb15.8. Lc. lactis MG1363 pGus clone 8 Touchdown PCR: Lb13.2.2 Lc. lactis MG1363 pJP059 Lb13.2.3 Lc. lactis MG1363 pJP059 Lb13.3 Lc. lactis MG1363 pJP059 Lb13.4 Lc. lactis MG1363 pJP059 Screening PCR: 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 Gel electrophoresis: Lb13.2.2 lactis MG1363 pJP059 (PCR with DMSO done yesterday) Lb13.2.3 lactis MG1363 pJP059 (PCR with DMSO done yesterday) Lb13.3 lactis MG1363 pJP059 (PCR with DMSO done yesterday) Lb13.4 Lc. lactis MG1363 pJP059 (PCR with DMSO done yesterday) Lb13.2.2 Lc. lactis MG1363 pJP059 (Touchdown-PCR today) Lb13.2.3 Lc. lactis MG1363 pJP059 (Touchdown-PCR today) Lb13.3 Lc. lactis MG1363 pJP059 (Touchdown-PCR today) Lb13.4 Lc. lactis MG1363 pJP059 (Touchdown-PCR today) 62.1 pSB1C3-CP8 (Screening-PCR today, was run two times) 62.2 pSB1C3-CP8 (Screening-PCR today, was run two times) 63.1 pSB1C3-CP11 (Screening-PCR today, was run two times) 63.2 pSB1C3-CP11 (Screening-PCR today, was run two times) 63.3 pSB1C3-CP11 (Screening-PCR today, was run two times) 63.4 pSB1C3-CP11 (Screening-PCR today, was run two times) 64.1.1 pSB1C3-CP29 (Screening-PCR today, was run two times) 64.1.2 pSB1C3-CP29 (Screening-PCR today, was run two times) 64.1.3 pSB1C3-CP29 (Screening-PCR today, was run two times) 64.1.4 pSB1C3-CP29 (Screening-PCR today, was run two times) 65.1.1 pSB1C3-CP30 (Screening-PCR today, was run two times) 65.1.2 pSB1C3-CP30 (Screening-PCR today, was run two times) 65.2.1 pSB1C3-CP30 (Screening-PCR today, was run two times) 65.2.2 pSB1C3-CP30 (Screening-PCR today, was run two times) 67.1.1 pSB1C3-CP44 (Screening-PCR today, was run two times) 67.1.2 pSB1C3-CP44 (Screening-PCR today, was run two times) 67.2.1 pSB1C3-CP44 (Screening-PCR today, was run two times) 67.2.2 pSB1C3-CP44 (Screening-PCR today, was run two times) 1. pSB1C3-red (digest used in transformation, cut with E and S) 7. pSB3K3-red (digest used in transformation, cut with E and P) 22. pSB1C3-B0032-BFP (digest used in transformation, cut with X and P) 42. pSB4S15 (digest used in transformation, cut with SalI and SacI) pSB3C17-BFP (digest used in transformation, cut with SalI and SacI) PCR purification: 13.2.2 Lc. lactis MG1363-pJP059 Re-streaks: 42. pSB4S15-red 61. pSB1C3-CP1, clone 1-8 (spread out 2/7) 62. pSB1C3-CP8, clone 4-8 (spread out yesterday) 63. pSB1C3-CP11, clone 4-8 (spread out yesterday) 64. pSB1C3-CP29, clone 4-8 (spread out yesterday) 65. pSB1C3-CP30, clone 4-8 (spread out yesterday) 66. pSB1C3-CP41, clone 1-8 (spread out 2/7) 67. pSB1C3-Cp44, clone 4-8 (spread out yesterday) 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 72. pSB3K3-CP30-B0032-BFP Lb1. reuteri CF48 pLR581 Lb2. reuteri 1063 pLUL631 Lb3. reuteri DSM 20016 pVS2 Lb4. reuteri 100-23 no plasmid Lb6. reuteri DSM 20016 pLUL63TsA8 Lb7. reuteri DSM 20016 pGFP Lb8. reuteri 100-23 pGT232 Lb12. reuteri DSM 20016 no plasmid Lb14. reuteri DSM 20016 pLUL631(B?) Lb5. plantarum 256 rifR pAMβ1 Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS Screening PCR and gel analysis: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS Overnight culture: 48. pSB1C3-B0034-His-STS 42. pSB4S15-red Gel of Crt Z Ligation of 38(religation of digest), 27(religation of digest), 51(religation of digest), 75, 76, 78 Transformation of 38(religated), 38, 27(religated), 27, 51(religated), 51, 75, 76, 78 O/N of 57. and 58. <h2>Results</h2> Screening PCR and gel analysis: 48.1 pSB1C3-B0034-His-STS, was successful 45. pSB1C3-B0034-4CL 47. pSB1C3-B0034-STS, failed. Gel of CrtZ was blank. PCR fail. Think up a new method. Transformation of 75, 76 and 78(from yesterday) failed. Redo with negative controll of both ligation(by religating the digested pEL-plasmids) and plasmids(from plasmidprepp). Negative controll of O/N 57. and 58. contaminated. O/N: Lb10.1. plantarum 256: NC ok, but weirdly looking pellets and supernatants. Lb11.1. plantarum 36E: NC ok, but weirdly looking pellets and supernatants. Lb12.1 reuteri DSM 20016: NC ok, but weirdly looking pellets and supernatants. → Look in microscope to see if contaminated. Assembly and transformation: 1. pSB1C3-red, as NC: Good growth 7. pSB3K3-red, as NC: No growth 42. pSB4S15-red, as NC: No growth 61. pSB1C3-CP1: Growth, discernable red and white colonies 62. pSB1C3-CP8: Growth, discernable red and white colonies 63. pSB1C3-CP11: Growth, discernable red and white colonies 64. pSB1C3-CP29: Growth, discernable red and white colonies 65. pSB1C3-CP30: Growth, discernable red and white colonies 66. pSB1C3-CP41: Growth, discernable red and white colonies 67. pSB1C3-Cp44: Growth, discernable red and white colonies 77 pSB4C15-red: Growth, but only white and blue colonies* *E. coli with the right plasmid should have become red. → Most seem ok, do restreaks! Test fluorescence of BFP-colonies from 2/7, get new 42’s from Erik Gullberg since not even our frozen stocks are red (as they should be). Gel electrophoresis: Lb13.2.2 lactis MG1363 pJP059 (yesterday) No bands Lb13.2.3 lactis MG1363 pJP059 (yesterday) --”-- Lb13.3 lactis MG1363 pJP059 (yesterday) --”-- Lb13.4 Lc. lactis MG1363 pJP059 (yesterday) --”-- Lb13.2.2 Lc. lactis MG1363 pJP059 (Touchdown) Right band (~2000) Lb13.2.3 Lc. lactis MG1363 pJP059 (Touchdown) No bands Lb13.3 Lc. lactis MG1363 pJP059 (Touchdown) --”-- Lb13.4 Lc. lactis MG1363 pJP059 (Touchdown) --”-- 62.1 pSB1C3-CP8 (Screening) * (see below) 62.2 pSB1C3-CP8 (Screening) * (see below) 63.1 pSB1C3-CP11 (Screening) * (see below) 63.2 pSB1C3-CP11 (Screening) * (see below) 63.3 pSB1C3-CP11 (Screening) * (see below) 63.4 pSB1C3-CP11 (Screening) * (see below) 64.1.1 pSB1C3-CP29 (Screening) * (see below) 64.1.2 pSB1C3-CP29 (Screening) * (see below) 64.1.3 pSB1C3-CP29 (Screening) * (see below) 64.1.4 pSB1C3-CP29 (Screening) * (see below) 65.1.1 pSB1C3-CP30 (Screening) * (see below) 65.1.2 pSB1C3-CP30 (Screening) * (see below) 65.2.1 pSB1C3-CP30 (Screening) * (see below) 65.2.2 pSB1C3-CP30 (Screening) * (see below) 67.1.1 pSB1C3-CP44 (Screening) * (see below) 67.1.2 pSB1C3-CP44 (Screening) * (see below) 67.2.1 pSB1C3-CP44 (Screening) * (see below) 67.2.2 pSB1C3-CP44 (Screening) * (see below) 1. pSB1C3-red (cut with E and S): Clear bands (safe to use) 7. pSB3K3-red (cut with E and P): Clear bands (safe to use) 22. pSB1C3-B0032-BFP (cut with X and P) No bands (get more data) 42. pSB4S15 (cut with SalI and SacI) No bands (get more data) pSB3C17-BFP (cut with SalI and SacI) Faint bands (likely safe) *Neither of the two runs on gel showed anything but weak, smudgy bands. Try again. PCR purification: Lb13.2.2 Lc. lactis MG1363-pJP059: 25.9 ng/µl Plasmid preparation: Lb9.7.1 faecalis JH2-2 pAMβ1 clone 7.1 (erythromycin): 140.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.7.2 faecalis JH2-2 pAMβ1 clone 7.2 (erythromycin): 116.0 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.8.1 faecalis JH2-2 pAMβ1 clone 8.1 (no-antibiotic): 76.6 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.8.2 faecalis JH2-2 pAMβ1 clone 8.2 (no-antibiotic): 55.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.9.1 faecalis JH2-2 pAMβ1 clone 9.1 (erythromycin): 231.9 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.9.2 faecalis JH2-2 pAMβ1 clone 9.2 (erythromycin): 96.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.10.1 faecalis JH2-2 pAMβ1 clone 10.1 (no-antibiotic): 113.2 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb9.10.2 faecalis JH2-2 pAMβ1 clone 10.2 (no-antibiotic): 107.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb13.5. Lc. lactis MG1363 pJP059 clone 5: 13.8 ng/µl → Dispose & don’t save in freezer. Lb13.6. Lc. lactis MG1363 pJP059 clone 6: 34.8 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb13.7. Lc. lactis MG1363 pJP059 clone 7: 25.9 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb13.8. Lc. lactis MG1363 pJP059 clone 8: 34.3 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb15.5. Lc. lactis MG1363 pGus clone 5: 5.9 ng/µl → Dispose & don’t save in freezer. Lb15.6. Lc. lactis MG1363 pGus clone 6: 9.7 ng/µl → Dispose & don’t save in freezer. Lb15.7. Lc. lactis MG1363 pGus clone 7: 15.7 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. Lb15.8. Lc. lactis MG1363 pGus clone 8: 16.7 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer. <h2>Other experiments</h2>TBE-mix Agarose gel mix EDTA mix Microscoping: *To see if the overnights from yesterday on strains 10 → 12 were contaminated. They showed to be that and the source are thought to be from a time ago, before frozen stock, since NC checked good for these overnights. </div>';

+

}

+

else if(id == 'd2013722')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-07-22</h1> Name of participants: Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Nils, Christoffer, Malin, Magnus, Niclas, Pontus, Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Mikael Strandgren (MS) <h2>Ongoing constructs</h2> 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 57.pSB1C3-B0034-Idi 99. pSB1C3-CrtO 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 127. pSB1A3-B0034-UGTCs2-His 21. CrtE~I /w RBS, Zinc finger (PCR prod) 128. pSIM5-tet(Nissle) 130. pSB1C3-CrtE~I /w RBS, Zinc Finger 131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger 132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger 133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger 75 . pEL3A15 - CrtB (LR) 76.pEL3K16-CrtI (LR) 79.pEL3K16- CrtE (LR) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 144.pSB1C3-B0034-UGTCs2-His 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 85. pSB4S15-CP25 84. pSB4S15-CP6 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 134.1 pSB1C3-CBP <h2>Todays work</h2> Restreaks: 115. pSB3T5-J23101-B0034-His-TAL* 116. pSB3T5-J23110-B0034-His-TAL* 117. pSB3T5-CP1-B0034-His-TAL* 137. pSB3K3-CP8-B0034-TAL* 138. pSB3K3-CP8-B0034-His-TAL* 139. pSB3K3-J23101-B0034-BFP* -*Restreaks from the transformation, that was done the day before Mutagenisis: 57.pSB1C3-B0034-Idi -> 129. pSB1C3-B0034-Idi (mut) 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 → Run PCR-products on gel to see if correct and then PCR clean it and prepare for sequencing. Phosphorylation of primers: usp45_mut_R usp45_mut_F Overnight: 127.1.pSB1A3-B0034-UGTCs2-His 127.2.pSB1A3-B0034-UGTCs2-His 86. pSB4S15-CP6-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15- CP11-B0032-BFP 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP Transformation: 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 81. pSB4S15-CP41-B0032-BFP 82. pSB4S15-CP29-B0032-BFP 83. pSB4S15-CP30-B0032-BFP 85. pSB4S15-CP25 Fluorescence(BFP) experiment with UV: 84. pSB4S15-CP6 86. pSB4S15-CP6-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP Screening: 131. pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger 132. pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger 133. pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger 75. pEL3A15 - CrtB 76. pEL3K16- CrtI 79. pEL3K16 - CrtE Digest 3.pSB3T5-red 92.BBa_J61002-J23110 90.BBa_J61002-J23101 127.pSB1A3-B0034-UGTCs2-His 51.pEL3C18-CP1 51.pEL3C18-CP8 58.pSB1C3-B0034-IspA 11.pSB4A15-red 91.BBa_J61002-J23106 94.pSB1C3-K206000 1.pSB1C3-red CSP_3 Plasmid 1.1 (from strain database 2013) Ligation: 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002-J23110 --> 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002-J23101 --> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 51.pEL3C18-CP1 --> 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 51.pEL3C18-CP8 --> 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 91.BBa_J61002-J23106 --> 108.pSBA15-J23106-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000 --> 109.pSBA15-K206000-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001 --> 110.pSBA15-K206001-B0034-IspA 127.pSB1A3-B0034-UGTCs2-His, 1.pSB1C3-red --> 144.pSB1C3-B0034-UGTCs2-His CSP_3 -Ligated with strain 1.1 (from strain database 2013) Let ligate in room temperature overnight. Frozen stock: (plasmid prep. tomorrow) 111.6 pSBLb4C15 111.7 pSBLb4C15 <h2>Results</h2> Gel electrophoresis: 79.pEL3K16 - CrtE looked ok on gel, but there was primer dimer contamination. 75.pEL3A15 - CrtB had several bands around 1500-3000. 76.pEL3K16-CrtI displayed no band except primer dimers. Digests looks good on gel Overnights 127.1* pSB1A3-B0034-UGTCs2-His 127.2* pSB1A3-B0034-UGTCs2-His -*did not grow.</div>';

+

}

+

else if(id == 'd2013723')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-07-23</h1> Name of participants: Nils, Christoffer, Malin, Magnus, Niclas, Pontus, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Christoffer Ahlström (CA), Nafisa Bashir (NB), Mikael Strandgren (MS), Stephanie Herman (SH), Alona Nyberg (AN) <h2>Ongoing constructs:</h2> 129. pSB1C3-B0034-Idi (mut) 108. pSB4A15-J23106-B0034-IspA 109. pSB4A15-K206000-B0034-IspA 110. pSB4A15-K206001-B0034-IspA 127. pSB1A3-B0034-UGTCs2-His 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 144.pSB1C3-B0034-UGTCs2-His 147. pSB1C3-B0034-Thioredoxin 152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 75.pEL3A15 - CrtB (LR) 76.pEL3K16-CrtI (LR) 79.pEL3K16 - CrtE (LR) 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 134. pSB1C3-CBP 111. pSBLb4C15 86. pSB4S15-CP6-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 136. pSB1C3-usp45 Lb9. faecalis JH2-2-pAMβ1 <h2>Todays work</h2> Plasmid preparation: 86. pSB4S15-CP6-B0032-BFP 97. pSB4S15-CP8-B0032-BFP 98. pSB4S15-CP11-B0032-BFP 111.6 pSBLb4C15 111.7 pSBLb4C15 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP 84. pSB4S15-CP6 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Mutagenesis: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 PCR: 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP Lb9. faecalis JH2-2-pAMβ1 clone 10.1* Lb9. faecalis JH2-2-pAMβ1 clone 10.2* -*With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R. To isolate ori. Digest: B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin Ligation: pSB1C3-B0034-Thioredoxin Restreak: 139. pSB3K3-J23101-B0032-BFP Transformation: 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 144.pSB1C3-B0034-UGTCs2-His 152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 135. pSB1C3-CSP* 148. pSB1C3-gfas purple 149. pSB1C3-meffred 150. pSB1C3-spispink 151. pSB1C3-meffblue -*Transformed into D5(alpha) competent cells. Gel electrophoresis: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 Screening: 129. pSB1C3-B0034-Idi (mut) 75.pEL3A15 - CrtE 76.pEL3K16 - CrtI 79.pEL3K16 - CrtB Overnight: 127.1.pSB1A3-B0034-UGTCs2-His 127.2.pSB1A3-B0034-UGTCs2-His 84. pSB4S15-CP6 87. pSB4S15-CP25-B0032-BFP 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP Spreading of strains: 135. pSB1C3-CSP* -*Two 1x dilutions were performed. Phosphorylation of primers: usp45_mut_R usp45_mut_F Frozen stock: 134.1 pSB1C3-CBP 134.2 pSB1C3-CBP 134.3 pSB1C3-CBP 134.4 pSB1C3-CBP <h2>Results</h2> Previous day: O/N: 134.1 pSB1C3-CBP: NC ok! 134.2 pSB1C3-CBP: NC ok! 134.3 pSB1C3-CBP: NC ok! 134.4 pSB1C3-CBP: NC ok! Today: Gel electrophoresis: 136.1 pSB1C3-usp45: Possible primer-dimer! 136.2 pSB1C3-usp45: Possible primer-dimer! 136.3 pSB1C3-usp45: Possible primer-dimer! → Re-do PCR mutagenesis with some edits. 129. pSB1C3-B0034-Idi (mut) did not show on gel 76.pEL3K16-CrtI displayed no band on gel. 75.pEL3A15 - CrtB displayed a band, lager than expected. 79.pEL3K16 - CrtE displayed a band at the right size (screened without dmso) and a good band with some contamination when screened with 7% dmso. Plasmid preparation: 134.1 pSB1C3-CBP: 126.0 ng/µl → Do a sequencing preparation. 134.2 pSB1C3-CBP: 124.9 ng/µl 134.3 pSB1C3-CBP: 126.6 ng/µl 134.4 pSB1C3-CBP: 126.2 ng/µl 111.6 pSBLb4C15: 33.7 ng/µl 111.7 pSBLb4C15: 52.2 ng/µl Transformation: 109.pSBA15-K206000-B0034-IspA - did not grow 147. pSB1C3-B0034-Thioredoxin - did not grow <h2>Other experiments</h2> 10x TBE-Buffer 10% Glycerol solution Sequencing preparation: 111.6. pSBLb4C15-red 111.7. pSBLb4C15-red 134.1 pSB1C3-CBP Primer dilution (from 100 µM to 5 µM): Ori-scr_F pL1-scr_F2 pL1-scr_F3 pL3-scr_F2 pL3-scr_F3 pAM-M1_F pAM-s-X1_R pAM-d-M1_R</div>';

+

}

+

else if(id == 'd2013724')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-07-24</h1> Name of participants: Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Nafisa Bashir (NB), Mikael Strandgren (MS), Jens Isaksson (JI), Malin, Magnus, Pontus, Nils, Niclas, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H. <h2>Ongoing constructs:</h2> 127.1.pSB1A3-B0034-UGTCs2-His 127.2.pSB1A3-B0034-UGTCs2-His 108. pSBA15-J23106-B0034-IspA 110. pSBA15-K206001-B0034-IspA 140. pSB3T5-J23110-B0034-UGTCs2-6xHis 141. pSB3T5-J23101-B0034-UGTCs2-6xHis 142. pSB3T5-CP1-B0034-UGTCs2-6xHis 143. pSB3T5-CP8-B0034-UGTCs2-6xHis 144. pSB1C3-B0034-UGTCs2-His 152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 90. BBa_J61002-J23101 153. pSB1C3-J23110-meffBlue 154. pSB1C3-J23106-meffBlue 155. pSB1C3-J23110-meffRed 156. pSB1C3-J23106-meffRed 157. pSB1C3-J23110-spisPink 158. pSB1C3-J23106-spisPink 159. pSB1C3-J23110-gfasPurple 160. pSB1C3-J23106-gfasPurple 66. pSB1C3-CP41 98. pSB4S15-CP11-B0032-BFP 22. pSB1C3-B0032-BFP 87. pSB4S15-CP25-B0032-BFP 84. pSB4S15-CP6 88. pSB4S15-CP1-B0032-BFP 89. pSB4S15-CP44-B0032-BFP 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-HIS-4CL 121. pSB1C3-B0034-STS 121. pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP <h2>Todays work</h2> Overnight culture: 68. pSB3K3-CP1-B0032-BFP 73. pSB3K3-CP41-B0032-BFP Ligation: 139. pSB3K3-J23101-B0032-BFP 162. pSB1C3-J23101 B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin 125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163.pSB3T5-ZCD-His-B0034-UGTCs2-His Lambda Red: 164.E-coli Nissle-CrtY Transformation: 90. BBa_J61002-J23101 153. pSB1C3-J23110-meffBlue 154. pSB1C3-J23106-meffBlue 155. pSB1C3-J23110-meffRed 156. pSB1C3-J23106-meffRed 157. pSB1C3-J23110-spisPink 158. pSB1C3-J23106-spisPink 159. pSB1C3-J23110-gfasPurple 160. pSB1C3-J23106-gfasPurple 147. pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow Sequencing preparation: 66. pSB1C3-CP41 98. pSB4S15-CP11-B0032-BFP 127.1.pSB1A3-B0034-UGTCs2-His Digestion: 22. pSB1C3-B0032-BFP 90. BBa_J61002(amp)-J23101 125.1 pSB1C3-ZCD-His Gel electrophoresis: Lb9. faecalis JH2-2 pAMβ1 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 Re-streak: 108. pSBA15-J23106-B0034-IspA 110. pSBA15-K206001-B0034-IspA 140. pSB3T5-J23110-B0034-UGTCs2-6xHis 141. pSB3T5-J23101-B0034-UGTCs2-6xHis 142. pSB3T5-CP1-B0034-UGTCs2-6xHis 143. pSB3T5-CP8-B0034-UGTCs2-6xHis 144. pSB1C3-B0034-UGTCs2-His 152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 135.1 pSB1C3-CSP 135.2 pSB1C3-CSP 135.3 pSB1C3-CSP 135.4 pSB1C3-CSP Mutagenesis: 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 PCR: Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1 Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2 -New temperatures (-3*C), new polymerase and new primer dilutions. With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R. To isolate ori. Plasmid Prep: 84.1 pSB4S15-CP6 84.2 pSB4S15-CP6 87.1 pSB4S15-CP25-B0032-BFP 87.2 pSB4S15-CP25-B0032-BFP 88.1 pSB4S15-CP1-B0032-BFP 88.2 pSB4S15-CP1-B0032-BFP 89.1 pSB4S15-CP44-B0032-BFP 89.2 pSB4S15-CP44-B0032-BFP 127.1.pSB1A3-B0034-UGTCs2-His 127.2.pSB1A3-B0034-UGTCs2-His Screening PCR and gel electrophoresis on: 115. pSB3T5-J23101-B0034-His-TAL clone: 1, 2, 3 ,4 116. pSB3T5-J23110-B0034-His-TAL clone: 1, 2, 3, 4 117. pSB3T5-CP1-B0034-His-TAL clone: 1, 2, 3, 4 137. pSB3K3-CP8-B0034-TAL clone: 1, 2, 3, 4 138. pSB3K3-CP8-B0034-His-TAL clone: 1, 2, 3, 4 139. pSB3K3-J23101-B0034-BFP clone: 1, 2, 3, 4 Sequence prep on: 116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4 137. pSB3K3-CP8-B0034-TAL clone: 1, 4 Overnight culture on: 116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4 137. pSB3K3-CP8-B0034-TAL: 1, 4 Measurement of standards of p-coumaric acid in the shimadzu UV160, plotting of a standard graph. <h2>Results</h2> Previous day: Spreading of strains: 135. pSB1C3-CSP: Both white and red colonies. *Two 1x dilutions were performed. → Re-streak with 4 colonies. PCR: One of the PCR-machines (with 2x MluI-pAM) -were accidentally paused right after start last night, and so it had to be re-done this morning. Since the samples stood in room temperature for about 15 hour the polymerase may have lost it’s activity, a gel will tell. Today: Gel electrophoresis: Lb9.10.1 faecalis JH2-2 pAMβ1 with XbaI and MluI: Not even primer-bands Lb9.10.2 faecalis JH2-2 pAMβ1 with 2x MluI: -”- Lb9.10.1 faecalis JH2-2 pAMβ1 with XbaI and MluI: -”- Lb9.10.2 faecalis JH2-2 pAMβ1 with 2x MluI: -”- 136.1 pSB1C3-usp45: Possible primer-dimer and a mark at ~1000bp. 136.2 pSB1C3-usp45: Possible primer-dimer. 136.3 pSB1C3-usp45: Possible primer-dimer. 136.4 pSB1C3-usp45: Possible primer-dimer. → Re-do PCR with other temperatures! Plasmid Prepp: 84.1 pSB4S15-CP6: 59.3 ng/µl 84.2 pSB4S15-CP6: 66.7 ng/µl 87.1 pSB4S15-CP25-B0032-BFP: 12.6 ng/µl 87.2 pSB4S15-CP25-B0032-BFP: 52.0 ng/µl 88.1 pSB4S15-CP1-B0032-BFP: 508.2 ng/µl 88.2 pSB4S15-CP1-B0032-BFP: 489.7 ng/µl 89. pSB4S15-CP44-B0032-BFP: 7.4 ng/µl digest of B0034-Thioredoxin (Synthetic construct) looks good on gel Restreak of 143. pSB3T5-CP8-B0034-UGTCs2-6xHis did not grow ligation of 141. pSB3T5-J23101-B0034-UGTCs2-6xHis is wrong, since 92.BBa_J61002-J23101 was digested wrong Construct 116.3, 116.4, 137.1, 137.4 successfull. <h2>Other experiments</h2> Primer dilutions (from 100µM to 5 µM): pAM-M1_F pAM-M1_R pAM-X1_R </div>';

+

}

+

else if(id == 'd2013725')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-07-25</h1> Name of participants: Malin, Magnus, Niclas, Pontus, Nils, Nafisa Bashir (NB), Viktor Blomkvist (VB), Jens Isaksson (JI), Mikael Strandgren (MS), Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L <h2>Ongoing constructs</h2> E. coli: 108.pSBA15-J23106-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 144.pSB1C3-B0034-UGTCs2-His 147.pSB1C3-B0034-Thioredoxin 152.pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 164.E-coli Nissle-CrtY 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow 135. pSB1C3-CSP 136.1 pSB1C3-usp45 148. pSB1C3-gfas purple 149. pSB1C3-meffred 150. pSB1C3-spispink 151. pSB1C3-meffblue 162. pSB1C3-J23101 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP E. faecalis: 9. JH2-2-pAMβ1 clone <h2>Todays work</h2> O/N: 135. pSB1C3-CSP Transformation: 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 143. pSB3T5-CP8-B0034-UGTCs2-6xHis 162. pSB1C3-J23101 Restreak: 139. pSB3K3-J23101-B0032-BFP 148. pSB1C3-gfas purple 149. pSB1C3-meffred 150. pSB1C3-spispink 151. pSB1C3-meffblue 147. pSB1C3-B0034-Thioredoxin 164.E-coli Nissle-CrtY 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow Plasmid prep on: 116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4 137. pSB3K3-CP8-B0034-TAL clone: 1, 4 Gel electrophoresis of PCR products: Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1* Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2* 136.1 pSB1C3-usp45 136.2 pSB1C3-usp45 136.3 pSB1C3-usp45 136.4 pSB1C3-usp45 -*(With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R) PCR-purification: Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1* Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2* *With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R Screening: 108.3 pSBA15-J23106-B0034-IspA 108.4 pSBA15-J23106-B0034-IspA 110.1 pSBA15-K206001-B0034-IspA 140.1 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.2 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.3 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.4 pSB3T5-J23110-B0034-UGTCs2-6xHis 142.1 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.2 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.3 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.4 pSB3T5-CP1-B0034-UGTCs2-6xHis 144.1 pSB1C3-B0034-UGTCs2-His 144.2 pSB1C3-B0034-UGTCs2-His 144.3 pSB1C3-B0034-UGTCs2-His 144.4 pSB1C3-B0034-UGTCs2-His Overnight: 144.1 pSB1C3-B0034-UGTCs2-His 144.2 pSB1C3-B0034-UGTCs2-His 144.3 pSB1C3-B0034-UGTCs2-His 144.4 pSB1C3-B0034-UGTCs2-His 152.1 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.2 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.3 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.4 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His Ligation: 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 103.pSB1K3-J23101--> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis Digest and assembly: pL3E1 (pAMβ1 double) pL3 (pAMβ1 single) 105. pSB3T5-J23101-B0034-TAL 115. pSB3T5-J23101-B0034--His-TAL Transformation on construct: 105. pSB3T5-J23101-B0034-TAL 115. pSB3T5-J23101-B0034--His-TAL <h2>Results</h2> Screening of all clones failed except for: 144.1 pSB1C3-B0034-UGTCs2-His 144.2 pSB1C3-B0034-UGTCs2-His 144.3 pSB1C3-B0034-UGTCs2-His 144.4 pSB1C3-B0034-UGTCs2-His Today: Gel electrophoresis: Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1: Clear and correct bands, at ~1700 bp.* Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2: Clear and correct bands, at ~1700 bp.* 136.1 pSB1C3-usp45: Big smudge between approx. 2500 and 200 bp 136.2 pSB1C3-usp45: Empty 136.3 pSB1C3-usp45: Empty 136.4 pSB1C3-usp45: Empty *(With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R) <h2>Other experiments</h2> 75. Digest to check for wrong bindings Making LB-agar plates Spectrophotomertry of 106.5, 106.6 and 43.7, using the shimadzu UV160. -Inconclusive spectrophotometry results.</div>';

+

}

+

else if(id == 'd2013726')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-07-26</h1> Name of participants: Nafisa Bashir (NB), Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Mikael Strandgren (MS), Viktor Blomkvist (VB), Jens Isaksson (JI), Malin, Magnus, Niclas, Pontus, Nils, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L <h2>Ongoing constructs</h2> 148. pSB1C3-gfas purple 149. pSB1C3-meffred 150. pSB1C3-spispink 151. pSB1C3-meffblue 90. BBa_J61002-J23101 153. pSB1C3-J23110-meffBlue 154. pSB1C3-J23106-meffBlue 155. pSB1C3-J23110-meffRed 156. pSB1C3-J23106-meffRed 157. pSB1C3-J23110-spisPink 158. pSB1C3-J23106-spisPink 159. pSB1C3-J23110-gfasPurple 160. pSB1C3-J23106-gfasPurple 162. pSB1C3-J23101 168. pSBLbC2 (working name) 169. pSBLbEcC2 (working name) 143. pSB3T5-CP8-B0034-UGTCs2-6xHis 144.pSB1C3-B0034-UGTCs2-His 152.pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 147. pSB1C3-B0034-Thioredoxin 164.E-coli Nissle-CrtY 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP <h2>Todays work</h2> Screening: 108.3 pSBA15-J23106-B0034-IspA 108.4 pSBA15-J23106-B0034-IspA 110.1 pSBA15-K206001-B0034-IspA 140.1 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.2 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.3 pSB3T5-J23110-B0034-UGTCs2-6xHis 140.4 pSB3T5-J23110-B0034-UGTCs2-6xHis 142.1 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.2 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.3 pSB3T5-CP1-B0034-UGTCs2-6xHis 142.4 pSB3T5-CP1-B0034-UGTCs2-6xHis 147. pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow Re-streak: 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 143. pSB3T5-CP8-B0034-UGTCs2-6xHis 105. pSB3T5-J23101-B0034-TAL 115. pSB3T5-J23101-B0034--His-TAL Plasmid prep: 144.1 pSB1C3-B0034-UGTCs2-His 144.2 pSB1C3-B0034-UGTCs2-His 144.3 pSB1C3-B0034-UGTCs2-His 144.4 pSB1C3-B0034-UGTCs2-His 152.1 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.2 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.3 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His 152.4 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His Transformation*: 168. pSBLbC2 (ori from pSB4C15 replaced with ori from pAMβ1) 169. pSBLbEcC2 (ori from pAMβ1 added to pSB4C15) * Not spread. Ligation: 3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 103.pSB1K3-J23101--> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis <h2>Results:</h2> Screening showed incorrect results on all constructs, but possible sources for error in the analysis made these results inconclusive. A new screening was set to run overnight. <h2>Other experiments</h2> Organization of frozen stock 75. Digest to check for wrong bindings Two phase separation of p-coumaric acid with heptane. Spectrophotometry. --Heptane proved not to be so good for the separation.</div>';

+

}

+

else if(id == 'd2013727')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-07-27</h1> Name of participants: Kristoffer L, Marcus H., Stephanie Herman (SH), Nafisa Bashir (NB), Malin, Magnus, Pontus, Nils <h2>Ongoing constructs</h2> 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 90. BBa_J61002-J23101 148. pSB1C3-gfas purple 149. pSB1C3-meffred 150. pSB1C3-spispink 151. pSB1C3-meffblue 153. pSB1C3-J23110-meffBlue 154. pSB1C3-J23106-meffBlue 155. pSB1C3-J23110-meffRed 156. pSB1C3-J23106-meffRed 157. pSB1C3-J23110-spisPink 158. pSB1C3-J23106-spisPink 159. pSB1C3-J23110-gfasPurple 160. pSB1C3-J23106-gfasPurple 162. pSB1C3-J23101 <h2>Todays work</h2> Digest on construct: 106. pSB3T5-J23110-B0034-TAL 104. pSB1C3-B0034-4CL-B0034-STS Assembly on cnstruct: 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS Transformation on construct: 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS Plasmid Preperation: 148.1 pSB1C3-gfas purple 148.2 pSB1C3-gfas purple 149.1 pSB1C3-meffred 149.4 pSB1C3-meffred 150.1 pSB1C3-spispink 150.2 pSB1C3-spispink 151.2 pSB1C3-meffblue Overnight culture: 90. BBa_J61002-J23101 153. pSB1C3-J23110-meffBlue 154. pSB1C3-J23106-meffBlue 155. pSB1C3-J23110-meffRed 156. pSB1C3-J23106-meffRed 157. pSB1C3-J23110-spisPink 158. pSB1C3-J23106-spisPink 159. pSB1C3-J23110-gfasPurple 160. pSB1C3-J23106-gfasPurple 162. pSB1C3-J23101 <h2>Result</h2> Transformation on construct: 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS Failed. Retry assembly 110.pSBA15-K206001-B0034-IspA did not grow Gel electrophoresis: 147. pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow didn’t show on gel Plasmid Preperation: 148.1 pSB1C3-gfas purple: 311.6 ng/µl 148.2 pSB1C3-gfas purple: 338.0 ng/µl 149.1 pSB1C3-meffred: 202.4 ng/µl 149.4 pSB1C3-meffred: 310.4 ng/µl 150.1 pSB1C3-spispink: 305.4 ng/µl 150.2 pSB1C3-spispink: 323.8 ng/µl 151.2 pSB1C3-meffblue: 179.2 ng/µl </div>';

+

}

+

else if(id == 'd2013728')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-07-28</h1> Name of participants: Mikael Strandgren (MS), Alona Nyberg(AN), Kristoffer L, Marcus H, Karl H.,Malin, Magnus, Nils, Pontus <h2>Ongoing constructs</h2> E. coli: 168. pSBLbC2 169. pSBLbEcC2 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS <h2>Todays work</h2> PCR: 19. CrtE~(O)-Z/w RBS, ZF 20.CrtE~Z/w RBS, ZF 21. CrtE~I/wRBS,ZF Restreak: 140.pSB3T5-J23110-B0034-UGTCs2-His 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-His. Spreading of transformed E. coli (from 20130726): 90.1 BBa_J61002-J23101 153. pSB1C3-j23110-meffBlue 155. pSB1C3-j23110-meffRed 156. pSB1C3-j23106-meffRed 157. pSB1C3-j23110-spisPink 159. pSB1C3-j23110-gfasPurple 160. pSB1C3-j23106-gfasPurple 162. pSB1C3-J23101 168. pSBLbC2 169. pSBLbEcC2 Frozen stock & plasmid preparation: 90.1 BBa_J61002-J23101 90.2 BBa_J61002-J23101 153.1 pSB1C3-j23110-meffBlue 153.2 pSB1C3-j23110-meffBlue 155.1 pSB1C3-j23110-meffRed 156.1 pSB1C3-j23106-meffRed 157.1 pSB1C3-j23110-spisPink 157.2 pSB1C3-j23110-spisPink 159.4 pSB1C3-j23110-gfasPurple 160.3 pSB1C3-j23106-gfasPurple 160.4 pSB1C3-j23106-gfasPurple 162.1 pSB1C3-J23101 162.2 pSB1C3-J23101 Overnight: 106.5 pSB3K3-B0034-J23110-TAL 106.6 pSB3K3-B0034-J23110-TAL 43.7 pSB1C3-B0034-TAL 143.1.pSB3T5-CP8-B0034-UGTCs2-6xHis 167.1.pSB1C3-fwYellow 167.2.pSB1C3-fwYellow Digest: 5.pSB4K15-red digested with E,P 111.6.pSBLb4C15-red digested with E,P 152.1.pUC57(amp)-B0034-ExpTag-Linker-Miraculin-HisTag digested with E,P 152.1.pUC57(amp)-B0034-ExpTag-Linker-Miraculin-HisTag digested with X,P Assembly: 105. pSB3T5-J23101-B0034-TAL 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS Ligation: 58.pSB1C3-B0034-IspA, 5.pSB4K15-red, 91.BBa_J61002-J23106 --> 108.pSBA15-J23106-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000--> 109.pSBA15-K206000-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001 --> 110.pSBA15-K206001-B0034-IspA 3.pSB3T5-red, 125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(E,P), 1.pSB1C3-red-> 176.B1C3-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, 103.pSB1K3-J23101 -> 177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, 92.BBa_J61002(amp)_J23110 -> 178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, CP1 -> 179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, CP8 -> 180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP1 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP8 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His Transformation: 105. pSB3T5-J23101-B0034-TAL 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS Gel electrophoresis: 76. pEL3K16-CrtI 20. CrtZ 19.CrtE~(O)-Z 20.CrtE~Z 21. CrtE~I 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 147.pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow Sequence prep: 144. pSB1C3-B0034-UGTCs2-His <h2>Results</h2> Frozen stock & plasmid preparation: 90.1 BBa_J61002-J23101: 215.4 ng/µl 90.3 BBa_J61002-J23101: 210.1 ng/µl 153.1 pSB1C3-j23110-meffBlue: 112.6 ng/µl 153.2 pSB1C3-j23110-meffBlue: 119 ng/µl 155.1 pSB1C3-j23110-meffRed: 169.5 ng/µl 156.1 pSB1C3-j23106-meffRed: 200.3 ng/µl 157.1 pSB1C3-j23110-spisPink: 73.7 ng/µl 157.2 pSB1C3-j23110-spisPink: 222.1 ng/µl 159.4 pSB1C3-j23110-gfasPurple: 279.7 ng/µl 160.3 pSB1C3-j23106-gfasPurple: 116.1 ng/µl 160.4 pSB1C3-j23106-gfasPurple: 122.8 ng/µl 162.1 pSB1C3-J23101: 79.9 ng/µl 162.2 pSB1C3-J23101: 80.1 ng/µl Gel electrophoresis: 76. pEL3K16-CrtI - Correct at 50 degrese tube 20. CrtZ - Correct for low temps 19.CrtE~(O)-Z - weak incorrect bands 20.CrtE~Z - weak incorrect bands 21. CrtE~I - 3 bands, one correct 2 wrong 143. pSB3T5-CP8-B0034-UGTCs2-6xHis - 143.1 success, other clones failed 163.pSB3T5-ZCD-His-B0034-UGTCs2-His - Failed 167.1.pSB1C3-fwYellow - Success 167.2.pSB1C3-fwYellow - Success </div>';

+

}

+

else if(id == 'd2013729')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-07-29</h1> Name of participants: Malin, Magnus, Pontus, Nils, Christoffer, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alona Nyberg(AN), Nafisa Bashir (NB), Mikael Strandgren (MS), Jens Isaksson (JI) <h2>Ongoing constructs</h2> 108.pSB4K15-J23106-B0034-IspA 109.pSB4A15-K206000-B0034-IspA 110.pSB4A15-K206001-B0034-IspA 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 176.B1C3-B0034-Exp-Linker-Miraculin-His 177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His 178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His 179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His 180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL Lb10. plantarum 256-p256 <h2>Todays work</h2> O/N, Frozen Stock: 76.pEL3K16-CrtI Screening & Overnight: 140.pSB3T5-J23110-B0034-UGTCs2-His 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-His. Digestion: 3. pSB3K3-red 36. pEL3S17-red 106. pSB3T5-J23110-B0034-TAL Assembly: 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL Transformation: 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 163.pSB3T5-ZCD-His-B0034-UGTCs2-His 176.B1C3-B0034-Exp-Linker-Miraculin-His 177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His 178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His 179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His 180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL Lb10. plantarum 256 p256 (as competence test) Lb11. plantarum 256 plasmid free (as positive control) Lb12. reuteri DSM-20016 plasmid free (as positive control) Lb23. reuteri 100-23-pSBLb4C15-red (single-ori (pJP059) shuttle vector) Lb24. reuteri DSM-20016-pSBLb4C15-red (--”--) Lb25. reuteri 100-23-pSBLbC2-red (single-ori (pAMbeta1) shuttle vector) Lb28. reuteri 100-23-pVS2-red (as competence test) Restreak: 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL Ligation: 120. pSB1C3-B0034-His-4CL 173. pSB3T5-CP1-B0034-4CL-B0034-STS Gel: 147.pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow 19. CrtE~(O)-Z/w RBS, ZF 20. CrtE~Z/w RBS, ZF + gelextraction 21. CrtE~I/w RBS, ZF Lb10. plantarum 256 p256 169. pSBLbEcC2-red (20 clones)* -* With the purpose of trying to find clones transformed with plasmids containing the pAMbeta1-ori as well as the E. coli ori. Digest, ligate and transformation: 90.1 BBa_J61002-J23101 to 139. pSB3K3-J23101-B0032-BFP Two phase separation with octanol and acetic acid. Spectrophotometry of 106.5 and 106.6. Comparison between the Shimadzu UV160, Shimadzu UV1800, and the nanodrop. <h2>Results</h2> Gel: 147.pSB1C3-B0034-Thioredoxin - success 165.pSB1C3-tsPurple - success 166.pSB1C3-scOrange - success 167.pSB1C3-fwYellow - success 19. CrtE~(O)-Z/w RBS, ZF - No right band 20. CrtE~Z/w RBS, ZF - Right band slightly above 10000bp, thou weak and others, 21. CrtE~I/w RBS, ZF - Right band at around 8000bp All tranformations but 176 failed. Ligatoins will be redone, but with pSB3T5 replaced with pEL3S17, as this backbone has shown better performance. Transformation succes on construct: 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL </div>';

+

}

+

else if(id == 'd2013730')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-07-30</h1> Name of participants: Christoffer Ahlström (CA), Alona Nyberg (AN), Mikael Strandgren (MS), Malin, Magnus, Pontus, Nils, Christoffer, Sabri, Thorsteinn, Marcus H, Karl H, Theodor L, Emil M, Victor S <h2>Ongoing constructs:</h2> E.coli: 169. pSBLbEcC2-red 191. pSB1A2-fusionGFP 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 176.pSB1C3-B0034-Exp-Linker-Miraculin-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 104. pSB1C3-B0034-4CL-B0034-STS 105. pSB3T5-J23101-B0034-TAL 106. pSB3T5-J23110-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3T5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 117. pSB3T5-CP1-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 121.pSB1C3-B0034-STS 121.pSB1C3-B0034-His-STS 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 173. pSB3T5-CP1-B0034-4CL-B0034-STS 174. pSB3T5-CP8-B0034-4CL-B0034-STS 175. pSB3T5-J23101-B0034-4CL-B0034-STS 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL <h2>Todays work</h2> Transformation: 191. pSB1A2-fusionGFP 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 120. pSB1C3-B0034-His-4CL Spectrofotometry (using the nanodrop) of 106.5 and 106.6. Inconclusive results. Screening: 140.pSB3T5-J23110-B0034-UGTCs2-His 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-His. 171. pSB3T5-J23110-B0034-4CL-B0034-STS clone: 1,2,3,4 172. pSB3T5-CP1-B0034-TAL clone: 1,2,3,4 Plasmid preparation: 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 165.pSB1C3-tsPurple 166.pSB1C3-scOrange 167.pSB1C3-fwYellow PCR: 19. CrtE~(O)-Z/w RBS, ZF 20. CrtE~Z/w RBS, ZF Phosphorylation: 20. CrtE~Z/w RBS, ZF 21. CrtE~I/w RBS, ZF Streaking of strains: 191. pSB1A2-fusionGFP Re-streak: 139. pSB3K3-J23101-B0032-BFP 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 176.pSB1C3-B0034-Exp-Linker-Miraculin-His Ligate and transformation: 90. BBa_J61002-J23101 to 139. pSB3K3-J23101-B0032-BFP Digest and ligate: 162. pSB1C3-J23101 to 192.pSB3K3-J23101-B0032-BFP 58.pSB1C3-B0034-IspA, 5.pSB4K15-red, 91.BBa_J61002-J23106 --> 108.pSBA15-J23106-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000--> 109.pSBA15-K206000-B0034-IspA 58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001 --> 110.pSBA15-K206001-B0034-IspA 36. pEL3S17-red, 125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163. pEL3S17-ZCD-His-B0034-UGTCs2-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 92.BBa_J61002(amp)_J23110 -> 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, CP1 -> 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, CP8 -> 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP1 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP8 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His O/N, Frozen Stock: 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP Gel electrophoresis on: 171. pSB3T5-J23110-B0034-4CL-B0034-STS clone: 1,2,3,4 172. pSB3T5-CP1-B0034-TAL clone: 1,2,3,4 <h2>Results</h2> Previous day: Transformation: Lb10. plantarum 256 p256 growing, possibly contaminated Lb11. plantarum 256 plasmid free growing, possibly contaminated Lb12. reuteri DSM-20016 plasmid free growing, possibly contaminated Lb23. reuteri 100-23-pSBLb4C15-red: no growth Lb24. reuteri DSM-20016-pSBLb4C15-red no growth Lb25. reuteri 100-23-pSBLbC2-red no growth Lb28. reuteri 100-23-pVS2-red no growth → Do new competent cells and transformation immediately Today: Screening-pcr/Gel electrophoresis: 169. pSBLbEcC2-red (20 clones): No correct bands, just smudges. → Screen 20 new colonies for correct plasmid, use phusion polymerase and pEL-primers, giving some kind of (bigger) fragments regardless of whether the clone has the correct plasmid or not. </div>';

+

}

+

else if(id == 'd2013731')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-07-31</h1> Name of participants: Magnus, Pontus, Sabri, Thorsteinn, Nils, Malin, Alona Nyberg (AN), Christoffer Ahlström (CA), Anton Berglund (AB), Mikael Strandgren (MS), Jens Isaksson (JI), Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E <h2>Ongoing constructs</h2> E.coli: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 169. pSBLbEcC2-red 191. pSB1A2-fusionGFP 139. pSB3K3-J23101-B0032-BFP 192.pSB3K3-J23101-B0032-BFP 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL L. reuteri: 4.1 100-23 no plasmid 12.1 DSM 20016 no plasmid <h2>Todays work</h2> Screening: 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 176.pSB1C3-B0034-Exp-Linker-Miraculin-His 169. pSBLbEcC2-red (20 clones) Overnight: 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 176.pSB1C3-B0034-Exp-Linker-Miraculin-His Lb4.1 reuteri 100-23 no plasmid Lb12.1 reuteri DSM 20016 no plasmid 172. pSB3T5-CP1-B0034-TAL (clone 3) 188. pEL3S17-J23101-B0034-4CL-B0034-STS (clone 3 and 4) 189. pSB3K3-J23101-B0034-TAL (clone 2 and 3) Ligation: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002 (amp)-J23110 -> 140.pEL3S17-Red-J23110-B0034-UGTCs2-6xHis 36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis Transformation: 99. pCB1C3 - CrtO 190. pEL3K16 - CrtO 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pEL3S17-Red-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis 143.pSB3T5-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 139. pSB3K3-J23101-B0032-BFP 192.pSB3K3-J23101 Re-Streak: 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 191.1 pSB1A2-fusionGFP 191.2 pSB1A2-fusionGFP 191.3 pSB1A2-fusionGFP 191.4 pSB1A2-fusionGFP 139. pSB3K3-J23101-B0032-BFP 192.pSB3K3-J23101-B0032-BFP Gel: 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 [Linear from PCR mutagenesis of 19] 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 [linear from PCR mutagenesis of 20] Preparation for sequencing: 153. pSB1C3-j23110-meffBlue 155. pSB1C3-j23110-meffRed 156. pSB1C3-j23106-meffRed 157. pSB1C3-j23110-spisPink 159. pSB1C3-j23110-gfasPurple 160. pSB1C3-j23106-gfasPurple 150. pSB1C3-spispink 151. pSB1C3-meffblue 149. pSB1C3-meffred 148. pSB1C3-gfas purple Assembly: 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL PCR cloning and screening on gel of constructs: 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL <h2>Results</h2> Screening: 140.pSB3T5-J23110-B0034-UGTCs2-6xHis Failed 141.pSB3T5-J23101-B0034-UGTCs2-6xHis Failed 142.pSB3T5-CP1-B0034-UGTCs2-6xHis One colony succeded Tranformations of 108, 109, 110, 177, 141, 180, and 182 failed. Tranformations of 163, 178 and 179 succeeded. Transformations of 181 and 140 remain inconclusive. Screening of PCR products: 188.3 , 188.4, 189.2 and 189.3 have correct bands on the gel <h2>Other experiments</h2> LB-agar plates making: Ampicillin, 28 plates Chloramphenicol, 30 plates Kanamycin, 31 plates Spectinomycin, 26 plates Tetracycline, 29 plates </div>';

+

}

+

else if(id == 'd201381')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E <h2>Ongoing constructs:</h2>' +

+

'114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL '+

+

'137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS '+

+

'187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL '+

+

'<h2>Todays work</h2>PCR screening and gel electrophorersis of construct: 185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS '+

+

'<h2>sequencing prep. of constructs:</h2> 185.9-185.10 '+

+

'<h2>Transformation of constructs:</h2> 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL '+

+

'<h2>Results</h2>Construct 185, clone 9 and 10, bonds around 3000/4000 bp. '+

+

'<h2>Follow up</h2> - '+

+

'<h2>Other experiments</h2> - </div>';

+

}

+

else if(id == 'd201382')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-02</h1> Name of participants: Anton B, Christoffer A, Mikael S, Jens I, Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E, Magnus, Pontus, Sabri, Nils <h2>Ongoing constructs:</h2> E.coli: 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 108. pSBA15-J23106-B0034-IspA 109. pSBA15-K206000-B0034-IspA 110. pSBA15-K206001-B0034-IspA 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3T5-J23110-B0034-His-TAL 119. pSB1C3-B0034-4CL 120. pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 140. pSB3T5-J23110-B0034-UGTCs2-6xHis 141. pSB3T5-J23101-B0034-UGTCs2-6xHis 142. pSB3T5-CP1-B0034-UGTCs2-6xHis 163. pEL3S17-ZCD-His-B0034-UGTCs2-His 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 177. pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178. pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183. pEL3A15-B0034-CrtZ 184. pSB1C3-B0034-CrtZ 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 191. pSB1A2-fusionGFP 193. pSB1C3-amilGFP 194. pSB1C3-B0034-amilCP 195. pSB1C3-cjBlue 196. pSB1C3-eforRed 197. pSB1C3-aeBlue 198. pSB1C3-B0034-amilGFP 199. pSb1C3-amajCFP 200. pSB1C3-B0034-asCP 202. pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203. pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 204. pEL3S17-J23110-HIS-STS 205. pSBLB4C15-CP1-B0034-HIS-TAL 206. pSB1C3-J23101-B0034-TAL 207. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His L. reuteri: 3. DSM 20016 pVS2 4. 100-23 no plasmid 12. DSM 20016 no plasmid 23. 100-23 pSBLb4C15-red 24. DSM 20016 pSBLb4C15-red 26. 100-23 pSBLbEcC2-red 27. DSM 20016 pSBLbEcC2-red 28. 100-23 pVS2 <h2>Todays work</h2> Plasmid preparation: 139.1.1 pSB3K3-J23101-B0032-BFP 139.1.2 pSB3K3-J23101-B0032-BFP 191.1 pSB1A2-fusionGFP 191.2 pSB1A2-fusionGFP Ligation: 4.pSB1A3-Red, 147.pSB1C3-B0034-Thioredoxin, 125.pSB1C3-ZCD-His-> 208.pSB1A3-B0034-Thioredoxin-ZCD-His 36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis 152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His Streaking of plasmid-free cells from frozen stock as control of C-resistance: Lb4.1 reuteri. 100-23 no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak of construct: 171. pSB3T5-J23110-B0034-4CL-B0034-STS 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23101-B0034-TAL Screening: 99. pCB1C3 - CrtO 190. pEL3K16 - CrtO 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Overnight: 99. pCB1C3 - CrtO 190. pEL3K16 - CrtO 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Gel electrophoresis: PCR of 152. pUC57-B0034-Exp-Linker-Miraculin-Linker-His to extract Miraculin with biobrick prefix and suffix <h2>Results</h2> Today: Plasmid preparation: 139.1.1 pSB3K3-J23101-B0032-BFP: 51.9 ng/µl 139.1.2 pSB3K3-J23101-B0032-BFP: 53.6 ng/µl 191.1 pSB1A2-fusionGFP: 78.9 ng/µl 191.2 pSB1A2-fusionGFP: 74.1 ng/µl</div>'

+

}

+

else if(id == 'd201383')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1> Name of participants: Lovisa P, Magnus, Pontus <h2>Other experiments</h2> Making competent cells (D5α)</div>'

+

}

+

else if(id == 'd201383')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1> Name of participants: Lovisa P, Magnus, Pontus <h2>Other experiments</h2> Making competent cells (D5α)</div>'

+

}

+

else if(id == 'd201384')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-04</h1> Name of participants: Emil M, Magnus, Pontus <h2>Ongoing constructs:</h2> 108.pSBA15-J23106-B0034-IspA 109.pSBA15-K206000-B0034-IspA 110.pSBA15-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pSB3T5-J23101-B0034-UGTCs2-6xHis 142.pSB3T5-CP1-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His <h2>Todays work:</h2> Overnight: 10. D5α <h2>Characterization</h2> Detection of TAL activity through spectroscopy (day 1) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2Other experiments: Competent cell test </div>'

+

}

+

else if(id == 'd201385')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-05</h1> Name of participants: Anton B, Viktor T, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn <h2>Ongoing constructs:</h2> L. reuteri: 23. 100-23 pSBLb4C15-red 25. 100-23 pSBLbC2-red 28. 100-23 pVS2 E. coli: 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Todays work</h2> Competent cells Lb4.1 reuteri 100-23 no plasmid Lambda red transformation: Crt Transformation: reuteri 100-23 pSB1C3-red (1 tube, to see if it would work in Lb, died in electroporation) Lb23. reuteri 100-23 pSBLb4C15-red (5 tubes, 1 died in electroporation) Lb25. reuteri 100-23 pSBLbC2-red (1 tube) Lb28. reuteri 100-23 pVS2 (2 tubes, 1 died in electroporation) 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Screening: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI <h2>Characterization</h2> Detection of TAL activity through spectroscopy (day 2) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2>Other experiments</h2> Casting LB-agar plates: Kanamycin, 63 plates Chloramphenicol, 61 plates Spectinomycin, 65 plates Tetracycline, 32 plates Ampicillin, 30 plates Competent cells (D5α)</div>'

+

}

+

else if(id == 'd201386')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-06</h1> Name of participants: Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Anton B, Mikael S, Viktor T <h2>Ongoing constructs:</h2> E. coli: 57.3.pSB1C3-B0034-Idi 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 6. pSB1K3-red 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 111. pSBLb4C15-red 139. pSB3K3-J23101-B0032-BFP 165. pSB1C3-RBS-tsPurle 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 194. pSB1C3-B0034-amilCP 198. pSB1C3-B0034-amilGFP L. reuteri: 23. 100-23 pSBLb4C15-red 25. 100-23 pSBLbC2-red 28. 100-23 pVS2 <h2>Todays work:</h2> Transformation: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His Restreak: 110.pEL3S17-K206001-B0034-IspA 115. pSB3K5-J23101-B0034-His-TAL 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Digest: 6. pSB1K3-red 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 68.2 pSB3K3-CP1-B0032-BFP (EcoRI + PstI) 69.4 pSB3K3-CP8-B0032-BFP (EcoRI + PstI) 70.3 pSB3K3-CP11-B0032-BFP (EcoRI + PstI) 71.1 pSB3K3-CP29-B0032-BFP (EcoRI + PstI) 72.2 pSB3K3-CP30-B0032-BFP (EcoRI + PstI) 73.4 pSB3K3-CP41-B0032-BFP (EcoRI + PstI) 74. pSB3K3-CP44-B0032-BFP (EcoRI + PstI) 111. pSBLb4C15-red (XbaI + PstI) 165. pSB1C3-RBS-tsPurle (XbaI + PstI) 166. pSB1C3-RBS-scOrange (XbaI + PstI) 167. pSB1C3-RBS-fwYellow (XbaI + PstI) 194. pSB1C3-B0034-amilCP (XbaI + PstI) 198. pSB1C3-B0034-amilGFP (XbaI + PstI) Ligation: B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin B0034-Thioredoxin, 1. pSB1C3-red (X, P) -> 147. pSB1C3-B0034-Thioredoxin Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI Screening: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI 204.(1-4) pEL3S17-J23110-B0034-HIS-STS 171(1-4) pEL3S17-J23110-B0034-4CL-B0034-STS Mutagenesis: 43.7 pSB1C3-B0034-TAL 57.3.pSB1C3-B0034-Idi -> 129.pSB1C3-Idi(mut) <v>Sequencing preparation: 139.1 pSB3K3-J23101-B0032-BFP 185.9 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 185.10 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS O/N: 204.2 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) 111.7 pSBLb4C15-red <h2>Results:</h2> Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) failed 201.E.Coli Nissle-CrtY-CrtI failed Previous day: Transformation: No visual growth. Keeping them on growth for one more day. <h2>Characterization:</h2> Detection of TAL activity through spectroscopy (day 3) Contructs tested for TAL activity: 106.5 pSB3K3-J23110-B0034-TAL 106.6 pSB3K3-J23110-B0034-TAL 137.1 pSB3K3-CP8-B0034-TAL 189.3 pSB3K3-J23101-B0034-TAL Negative controls: 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL Positive controls (p-coumaric acid added before dextraction): 43.7 pSB1C3-B0034-TAL 119.2 pSB1C3-B0034-4CL <h2>Other Experiments:</h2> 10.D5Alpha competence test Preparation of buffers for Western Blot Casting LB-agar plates: Kanamycin, 33 plates Spectinomycin, 31 plates </div>'

+

}

+

else if(id == 'd201387')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-07</h1> Name of participants: - Viktor T, Anton B, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils <h2>Ongoing constructs:</h2> E. coli: 6. pSB1K3-red 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 92. J23110 111. pSBLb4C15-red 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple 241. pSB1K3-J23110-RBS-scOrange 242. pSB1K3-J23110-RBS-fwYellow 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple <h2>Todays work</h2> Assembly: pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple* 241. pSB1K3-J23110-RBS-scOrange* 242. pSB1K3-J23110-RBS-fwYellow* *Will eventually be tried in shuttle vector, but that has C-resistance, so a subcloning to K is necessary first, or a modification to change the resistance of the shuttle vector. Transformation: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 240. pSB1K3-J23110-RBS-tsPurple 241. pSB1K3-J23110-RBS-scOrange 242. pSB1K3-J23110-RBS-fwYellow 108.pEL3S17-J23106-B0034-IspA 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue Spreading: 60. PK401 dam- no plasmid 111.7 pSBLb4C15-red Restreak: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue O/N & Screening: 110.pEL3S17-K206001-B0034-IspA 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pBAD/AraC-RBS-Luciferas (2013 years kit) 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple Gel: 164.E.Coli Nissle-CrtY (clones from transformation plate) 201.E.Coli Nissle-CrtY-CrtI 129.pSB1C3-Idi(mut) <h2>Results</h2> Transformation (Monday): Still no visual growth. SDS-page failed (probably due to faulty buffers, investigate the running buffer first) <h2>Characterization experiments</h2> SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) <h2>Other experiments</h2> Sequencing preparation: 69. pSB3K3-CP8-B0032-BFP* *since the existing sequencing data doesn’t cover the whole insert with sufficient accuracy. There may be a mutation at the end of BFP, but that is more than 800bp after the single primer. Was not sent today, not sequencing tuesday.</div>'

+

}

+

else if(id == 'd201388')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-08</h1> Name of participants: Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Stephanie H, Mikael S, Anton B <h2>Ongoing constructs:</h2> E. coli: 60. Strain PK401 dam- no plasmid 111.7 pSBLb4C15-red 139. pSB3K3-J23101.B0032-BFP 91. BBa_J61002-J23106 92. BBa_J61002-J23110 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSb1C3-amajCFP 200.pSB1C3-B0034-asCP 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS <h2>Todays work:</h2> Transformation: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 no nr. pSB1c3-Thioredoxin no nr. pEL3S17-Thioredoxin pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink Re-streak: 108.pEL3S17-J23106-B0034-IspA 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue <bPlasmid prepp 226. pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pBAD/AraC-RBS-Luciferas (2013 years kit) 245. pSB1C3-J23110-B0034-amajCP(amajLime) 246. pSB1C3-J23110-b0034-gfasPurple O/N screening: 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His Two step Assembly 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow Overnight (screening) 1. pSB1C3-red O/N 60. Strain PK401 dam- no plasmid 111.7 pSBLb4C15-red Streak from frozen stock 3. pSB3T5-red 6. pSB1K3-red 42. pSB4S15-red 77. pSB4C15-red <h2>Results:</h2> One of the SDS-gels failed, resulting in no good gel to stain (faulty buffers, continue to search for sources of errors) The succesfull gel was prepared with control buffers. Transformation of promotors with BFP in the shuttle vector, for characterization in Lactobacillus: Some growth, only white colonies, will let them grow overnight again. <h2>Characterization experiments:</h2> Westernblot SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) Electro transfer over night Staining of control gel Detection of TAL activity through spectroscopy (day 4)</div>'

+

}

+

else if(id == 'd201389')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-09</h1> Name of participants: Stephanie H, Mikael S, Anton B, Anders E, Magnus, Pontus, Sabri, Malin, Alexander, Nils, Christoffer <h2>Ongoing constructs:</h2> 60. PK401 dam- no plasmid 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP 111.7 pSBLb4C15-red 91. BBa_J61002-J23106 92. BBa_J61002-J23110 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 244. pSB1C3-Promoter-RBS-Bleomysin 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow <h2>Todays work</h2> Streak from frozen stock: 60. PK401 dam- no plasmid 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP O/N: 60.3 PK401 dam- no plasmid* *The O/N made on thursday used MRS, so it had to be remade from restreaks. Plasmid preparation: 111.7 pSBLb4C15-red 220.pSB3K3-J23110-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Restreak: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin (E,P & E,X) 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His no nr. pEL3S17-Thioredoxin Amplification PCR 134. B0034-CBP 135. B0034-CSP 254. Gus 257. p-pem 258. pem Mutagenesis 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 O/N Screening: 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 221. pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue Send to Linköping, as plasmid prepp and agar stabb 226.pSB1C3-pBAD/AraC-RBS-Luciferas (2012 years kit) 227.pSB1C3-pBAD/AraC-RBS-Luciferas (2013 years kit) Transformation 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink <h2>Results</h2> Plasmid preparation: 111.7 pSBLb4C15-red: 30,6 ng/µl O/N: 221 failed, redo. O/N Screening: 108.pEL3S17-J23106-B0034-IspA - Screening gel fail 109.pEL3S17-K206000-B0034-IspA - Screening gel fail 142.pEL3S17-CP1-B0034-UGTCs2-6xHis - 143.pEL3S17-CP8-B0034-UGTCs2-6xHis - 163.pEL3S17-ZCD-His-B0034-UGTCs2-His - Screening gel fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His - 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 - Screening gel fail 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - Screening gel fail 219.pSB1C3-Miraculin - 221. pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI - Screening gel fail</div>'

+

}

+

else if(id == 'd2013810')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-10</h1> Name of participants: Anders Edlund, Marcus H, Hampus.E, Kristoffer L, Ken B-A, Nils, Alexander, Sabri, Pontus <h2>Ongoing constructs:</h2> E. coli: 134. B0034-CBP 135. B0034-CSP 254. Gus 257. p-Toxin-Antitoxin 258. Toxin-Antitoxin 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 114. pSB1C3-B0034-TAL(MUT) 105. pSB3T5-J23101-B0034-TAL 107. pSB3T5-CP1-B0034-TAL 115. pSB3K5-J23101-B0034-His-TAL 119.pSB1C3-B0034-4CL 120.pSB1C3-B0034-HIS-4CL 137. pSB3K3-CP8-B0034-TAL 138. pSB3K3-CP8-B0034-His-TAL 139. pSB3K3-J23101-B0034-BFP 171. pSB3T5-J23110-B0034-4CL-B0034-STS 172. pSB3T5-CP1-B0034-TAL 185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS 186. pEL3S17-CP1-B0034-4CL-B0034-STS 187. pEL3S17-CP8-B0034-4CL-B0034-STS 188. pEL3S17-J23101-B0034-4CL-B0034-STS 189. pSB3K3-J23101-B0034-TAL 204. pEL3S17-J23110-HIS-STS 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 224. pSB1C3-J23101-B0032-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His E. coli PK401 dam-: 60. no plasmid <h2>Todays work:</h2> Gel-electrophoresis 134. B0034-CBP* 135. B0034-CSP* 254. Gus 257. p-Toxin-Antitoxin 258. Toxin-Antitoxin *PCR 99% failed 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 115. pSB3K5-J23101-B0034-His-TAL 114. pSB1C3-B0034-TAL(MUT) 120.pSB1C3-B0034-HIS-4CL Amplification PCR 134. B0034-CBP 135. B0034-CSP ON: 60. E. coli dam- Assembly and subcloning: 205.pSBLB4C15-CP1-B0034-HIS-TAL 206.pSB1C3-J23110-B0034-TAL 225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS 259. pEL3S17-B0034-4CL-B0034-STS 127 + 103 + 36 -> 253. pEL3S17-J23101-B0034-UGTCs2-His 152 + 61 + 111 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 152 + 62 + 111 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 95 + 58 + 36 -> 110.pEL3S17-K206001-B0034-IspA CrtZ + 1 -> 184.pSB1C3-B0034-CrtZ Miraculin + 1 -> 219.pSB1C3-Miraculin O/N Screening: 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 219.pSB1C3-Miraculin 222.pSB3K3-CP1-B0034-UGTCs2-His 201. E.Coli. Nissle - CrtY-CrtI DPN1 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 <h2>Results:</h2> Gel electrophoresis: 115 good, to sequencing 114 good, to sequencing 120 pick new clones and screen 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 - Succes 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - fail 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 -fail Characterization experiments Detection of TAL activity through spectroscopy (day 5)</div>'

+

}

+

else if(id == 'd2013811')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-11</h1> Name of participants: Anders E, Mikael S, Nafisa B, Nils, Alexander, Sabri, Malin <h2>Ongoing constructs:</h2> E. coli: 60. PK401 dam- no plasmid 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 91. BBa_J61002-J23106 92. BBa_J61002-J23110 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 165. pSB1C3-RBS-tsPurple 166. pSB1C3-RBS-scOrange 167. pSB1C3-RBS-fwYellow 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 244. pSB1C3-Promoter-RBS-Bleomysin 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow 253. pEL3S17-J23101-B0034-UGTCs2-His E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work:</h2> Competent cells: 60. PK401 dam- noplasmid Transformation: 275. PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) Gel electrophoresis: 111.7 pSBLb4C15 cut with E and P and more. Digest: 111.7 pSBLb4C15 cut with E and P 111.7 pSBLb4C15 cut with SacI and SalI 254. Gus P+X 257. p-Toxin-Antitoxin P+X 258. Toxin-Antitoxin P+X 134. B0034-CBP E+S 1. pSB1C3-red E+S 3. pSB3T5-red E+P PCR purification: 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 134. pSB1C3-B0034-CBP PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) Plasmidprepp: 108.2.pEL3S17-J23106-B0034-IspA 108.3.pEL3S17-J23106-B0034-IspA 148. pSB1C3-RBS-gfasPurple 150. pSB1C3-RBS-spisPink 151. pSB1C3-RBS-meffBlue 163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 243. pSB1A2-LacI 244. pSB1C3-Promoter-RBS-Bleomysin 228.pEL3A17-Blue 229.pEL3K17-Blue 230.pEL3S15-Blue 231.pEL3C17-Blue Two part assembly: 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow O/N: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis Sekvensprepp: 220.pSB3K3-J23110-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His Re-circularization: 202.pSB4C5.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 Re-streak 165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple 165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple 166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange 166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange 167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow 167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink <h2>Results:</h2> Gel electrophoresis: 111.7 pSBLb4C15 cut with E and P: correct bands! and more.</div>'

+

}

+

else if(id == 'd2013812')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1> Name of participants: Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B <h2>Ongoing constructs:</h2> E. coli: 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 149. pSB1C3-B0034-Thioredoxin 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 114. pSB1C3-B0034-TAL-mut 115. pSB3K3-J23101-B0034-His-TAL 204. pEL3S17-J23110-B0034-HIS-STS 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work</h2> Ligation: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 134. pSB1C3-B0034-CBP 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Digest: 4.pSB1A3-red 6.pSB1K3 125. pSB1C3-ZCD-His 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Re-streak: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253. pEL3S17-J23101-B0034-UGTCs2-His 275 PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) O/N Screening: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp Transformation 134. pSB1C3-CBP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Spreading: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem Plasmidprepp: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Sequence prepp: 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Transformation: 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp <h2>Results:</h2> 222.1.pSB3K3-CP1-B0034-UGTCs2-His failed 222.3.pSB3K3-CP1-B0034-UGTCs2-His failed 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Mixing new buffers for western blot Casting LB-agar plates: Chloramphenicol: 55</div>'

+

}

+

else if(id == 'd2013812')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1> Name of participants: Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B <h2>Ongoing constructs:</h2> E. coli: 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 149. pSB1C3-B0034-Thioredoxin 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 114. pSB1C3-B0034-TAL-mut 115. pSB3K3-J23101-B0034-His-TAL 204. pEL3S17-J23110-B0034-HIS-STS 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work</h2> Ligation: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 134. pSB1C3-B0034-CBP 254. pSB1C3-Gus 257. pSB1C3-p-Toxin-Antitoxin (with promotor) 258. pSB1C3-Toxin-Antitoxin 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Digest: 4.pSB1A3-red 6.pSB1K3 125. pSB1C3-ZCD-His 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His Re-streak: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253. pEL3S17-J23101-B0034-UGTCs2-His 275 PK401 dam- pSBLb4C15-red (dam-) 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) O/N Screening: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp Transformation 134. pSB1C3-CBP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 233. pSBLb4C15-CP1-B0034-BFP 234. pSBLb4C15-CP8-B0034-BFP 235. pSBLb4C15-CP11-B0034-BFP 236. pSBLb4C15-CP29-B0034-BFP 237. pSBLb4C15-CP30-B0034-BFP 238. pSBLb4C15-CP41-B0034-BFP 239. pSBLb4C15-CP44-B0034-BFP Spreading: 68. pSB3K3-CP1-B0032-BFP 69. pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-CP44-B0032-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem Plasmidprepp: 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Sequence prepp: 129. pSB1C3-B0034-Idi (mut) 149. pSB1C3-B0034-Thioredoxin Transformation: 202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) PCR: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) CBP Mcherry Pem Pemp <h2>Results:</h2> 222.1.pSB3K3-CP1-B0034-UGTCs2-His failed 222.3.pSB3K3-CP1-B0034-UGTCs2-His failed 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed Gel electrophoresis: Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R) <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Mixing new buffers for western blot Casting LB-agar plates: Chloramphenicol: 55</div>'

+

}

+

else if(id == 'd2013813')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-13</h1> Name of participants: Anders Edlund, Mikael Strandgren, Anton Berglund, Viktor Törnblom, Viktor Blomkvist, Christoffer Ahlström, Stephanie Herman, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L. Lovisa P, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 114.(2-3) pSB1C3-B0034-TAL-mut 115.(1-3) pSB3K3-J23101-B0034-His-TAL 204.(1-3) pEL3S17-J23110-B0034-HIS-STS <h2>Todays work:</h2> O/N Screening glycerolstock: 110.pEL3S17-K206001-B0034-IspA 219.pSB1C3-Miraculin 253.pEL3S17-J23101-B0034-UGTCs2-His Assembly pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink Ligation: 134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product]) 134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product]) 255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product]) 255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product]) 256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product]) 256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product]) Plasmidprepp: 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His Sequencing prep: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 114.(2-3) pSB1C3-B0034-TAL-mut 115.(1-3) pSB3K3-J23101-B0034-His-TAL 204.(1-3) pEL3S17-J23110-B0034-HIS-STS 108.2.pEL3S17-J23106-B0034-IspA 108.3.pEL3S17-J23106-B0034-IspA 163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His 142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis 222.1.pSB3K3-CP1-B0034-UGTCs2-His 222.3.pSB3K3-CP1-B0034-UGTCs2-His Re-streaking: 134. pSB1C3-CBP (CBP1 [134.1] from digested plasmids [PCR-product]) 134. pSB1C3-CBP (CBP2 [134.2] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus1 [254.1] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus2 [254.2] from digested plasmids [PCR-product]) 254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]) 255. pSBLb4C15-pemp (pemp1 [257.1] from digested plasmids [PCR-product]) 255. pSBLb4C15-pemp (pemp2 [257.2] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem1 [258.1] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem2 [258.2] from digested plasmids [PCR-product]) 256. pSBLb4C15-pem (pem3 [258.3] from digested plasmids [PCR-product]) 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Re streak 14/8 or 15/8 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) Transformation & plating 134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product]) 134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product]) 254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product]) 255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product]) 255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product]) 256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product]) 225. pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 261. pSB3K3-J23110-B0034-HIS-4CL 120. pSB3K3-B0034-HIS-4CL 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 177pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 208. pSB1A3-B0034-Thioredoxin-ZCD-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His Re-streaking 254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]): 1 clone 255. pSBLb4C15-pemp (pemp3 [257.3] from digested plasmids [PCR-product]): 4 clones O/N: 275. pSBLb4C15-red (dam-) 276. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam) 277. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam) Gradient PCR: CBP (colony PCR from Lb.Reuteri) CSP (colony PCR from Lb.Reuteri) Mcherry (from plasmid preparation) 256. pSBLb4C15-pem <h2>Results</h2> One of the two gels from SDS page was succesfull, still have problems with the buffers <h2>Characterization</h2> Characterization of Resveratrol production through spectroscopy and HPLC Western blot SDS-page on constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL Electro transfer over night <h2>Other experiments</h2> Casting LB-agar plates: Chloramphenicol: 85 </div>'

+

}

+

else if(id == 'd2013814')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL E. coli PK401 dam-: 111.7 pSBLb4C15-red 181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <h2>Todays work:</h2> Re-streak: 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after) pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after) Re streak 15/8 or 16/8 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink O/N: 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP Streak from frozen stock: 111. pSBLb4C15-red Gradient touchdown colony PCR: CSP Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CSP (colony PCR from Lb.Reuteri) Mcherry (from plasmid preparation) 256. pSBLb4C15-pem PCR purification: M1 M2 M3 M4 CB1 CB2 256. pSBLb4C15-pem (P1) Digestion: M3 (x+p) CB1 (x+p) P1 (x+p) 111.7 pSB4C15-red (e+p) Bleomycin resistance cassette (SacI) pEL PCR product with e. coli ori (ClaI) Lb3 reuteri DSM 20016 pVS2 (ClaI) CP promoters with amilGFP, aeBlue,tsPurple Ligation: M3 with pSB1C3, pSBLB4C15 + CP8, CP1 CB1 with pSB1C3 P1 with pSB1C3 pVS2 origin of replication with pEL PCR-product, cut by ClaI CP promoters with amilGFP, aeBlue,tsPurple Frozen stock and plasmid preparation: 275.1 pSBLb4C15-red (dam-)* 275.2 pSBLb4C15-red (dam-) 276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-) 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-) *O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight. Transformation: 109.pEL3S17-K206000-B0034-IspA x 110.pEL3S17-K206001-B0034-IspA x 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x 184.pSB1C3-B0034-CrtZ x 208.pSB1A3-B0034-Thioredoxin-ZCD-His x 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x 267.psB3K3-K206000-B0034-IspA x 268.psB3K3-K206001-B0034-IspA x 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x 272 pSB1K3-B0034-Thioredoxin-ZCD-His x 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x Plasmid prepp: 280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version) <h2>Results:</h2> Gel Electrophoresis: CBP (colony PCR from Lb.Reuteri) CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!! CSP (colony PCR from Lb.Reuteri) CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) Mcherry (from plasmid preparation) M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!! 256. pSBLb4C15-pem: P1: 47.9o C (NC ok!) → Correct bands!! PCR purification: M1: 65.9 ng/µl → Store in freezer! M2: 200.6 ng/µl → Store in freezer! M3: 136.8 ng/µl → For digest! → Store in freezer! M4: 49.5 ng/µl → Store in freezer! CB1: 90.8 ng/µl → For digest! → Store in freezer! CB2: 56.7 ng/µl → Store in freezer! P1: 56.2 ng/µl → For digest! → Store in freezer! Plasmid preparation: 275.1 PK401 dam- pSBLb4C15-red (dam-): 13.5 ng/µl 275.2 PK401 dam- pSBLb4C15-red (dam-): 27.9 ng/µl 276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl 277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl Western blot: Transfer overnight not complete but continued with anyway Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Blotting and development of constructs: 43.7 pSB1C3-B0034-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 115. pSB3K3-J23101-B0034-His-TAL <h2>Other experiments:</h2> Making MRS-broth (2x600 ml) New buffers for western blot made </div>'

+

}

+

else if(id == 'd2013815')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1> Name of participants: Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Digestions: Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer) Bleomycin resistance cassette (SalI, FD buffer) O/N Screening glycerolstock: 110.pEL3S17-K206001-B0034-IspA 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Re-streak: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 184.pSB1C3-B0034-CrtZ 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 110.pEL3S17-K206001-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Plasmid prep: 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP Transformation: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-) 294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-) Ligations: pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI Spreading of strains: 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry E. coli PK401 dam-: 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry Screening PCR and gel electrophoresis <h2Results:</h2> Yesterdays buffers for western blot resulted in smear. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC <h2>Other experiments</h2> Making MRS-agar plates: 47 Chloramphenicol 47 Without antibiotics Testing yesterdays buffers for western blot. New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</div>'

+

}

+

else if(id == 'd2013816')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1> Name of participants: Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> O/N Screening & glycerolstock: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 184.pSB1C3-B0034-CrtZ 208. pSB1A3-B0034-Thioredoxin-ZCD-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red Digestion Erythromycin cassette amplicon from pLUL631 (SalI) Ligation pSB1C3-pemp 298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI)) 297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI)) Plasmid preparation: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp O/N: 204.1 pEL3S17-J23110-B0034-HIS-STS 204.3 pEL3S17-J23110-B0034-HIS-STS 204.4 pEL3S17-J23110-B0034-HIS-STS 254.1 pSB1C3-gus 254.2 pSB1C3-gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 255.3 pSBLB4C15-pemp 255.4 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 257.3 pSB1C3-pemp 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Transformation: 297. PK401 dam- pSBLb4B15-red 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 155pSB1C3-j23110-RBS-meffRed 156pSB1C3-j23106-RBS-meffRed 157pSB1C3-j23110-RBS-spisPink 158pSB1C3-j23106-RBS-spisPink 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 247pSB1C3-J23110-RBS-tsPurple 248pSB1C3-J23106-RBS-tsPurple 249pSB1C3-J23110-RBS-scOrange 250pSB1C3-J23106-RBS-scOrange 251pSB1C3-J23110-RBS-fwYellow 252pSB1C3-J23106-RBS-fwYellow 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS <h2>Results:</h2> Plasmid prep: 204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl 204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl 204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl 254.1 pSB1C3-gus: 6.7 ng/µl 254.2 pSB1C3-gus: 11.5 ng/µl 255.1 pSBLB4C15-pemp: 9.9 ng/µl 255.2 pSBLB4C15-pemp: 6.3 ng/µl 255.3 pSBLB4C15-pemp: 5.0 ng/µl 255.4 pSBLB4C15-pemp: 4.6 ng/µl Since the concentrations were so low a new set of overnight cultures were prepared. Spectophotometry: Inconclusive results. <h2>Characterization experiments</h2> Characterization of Resveratrol production through spectroscopy and HPLC Testing of new buffers for western blot. <h2>Other experiments</h2> Making MRS-broth (2x600 ml) Making LB-agar plates: 173 Chloramphenicol (chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.) 28 Kanamycin</div>'

+

}

+

else if(id == 'd2013817')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1> Name of participants: Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Restreak 254.3 pSB1C3-Gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 297. pSBLb4B15-red 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS 295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS Plasmid preparation: 254.1 pSB1C3-Gus 254.2 pSB1C3-Gus 255.1 pSBLB4C15-pemp 255.2 pSBLB4C15-pemp 257.1 pSB1C3-pemp 257.2 pSB1C3-pemp 184.pSB1C3-B0034-CrtZ 280. pSB1K3-J23110-B0034-aeBlue 281. pSB3K3-J23110-B0034-amilCP 282. pSB3K3-J23110-B0034-asCP Digest: 147.pSB1C3-B0034-Thioredoxin (X,P) Ligation: 147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red ---> 300.pEL3S17-J23106-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red ---> 301.pEL3S17-J23110-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red ---> 299.pEL3S17-J23101-B0034-Thioredoxin Transformation: 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Results:</h2> Plasmid preparation: 254.1 pSB1C3-Gus 110 ng/uL 254.2 pSB1C3-Gus 132 ng/uL 255.1 pSBLB4C15-pemp156 ng/uL 255.2 pSBLB4C15-pemp 158 ng/uL 257.1 pSB1C3-pemp 146 ng/uL 257.2 pSB1C3-pemp 140 ng/uL</div>'

+

}

+

else if(id == 'd2013818')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1> Name of participants: Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H. <h2>Ongoing constructs:</h2> <h2>E. coli:</h2> 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> ON: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry pSBLB4C15-red pSBLB4C15-red dam- Restreak: 68.1 pSB3K3-B0034-BFP 68.2 pSB3K3-B0034-BFP 68.4 pSB3K3-B0034-BFP 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.pSB1C3-B0034-Thioredoxin 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.pSB3K3-K206000-B0034-IspA 268.pSB3K3-K206001-B0034-IspA 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.pEL3S17-J23106-B0034-Thioredoxin PCR: 134. pSB1C3-B0034-CBP 254. pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Digest: 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Ligation: 4.pSB1A3-red 7.pSB3K3-red 36.pEL3S17-red 91.j23106 92.j23110 103.pSB1K3-j23101 125. pSB1C3-ZCD-His 127. pSB1A3-B0034-UGTCs2-His 129.pSB1C3-Idi(mut) 184.pSB1C3-B0034-CrtZ Plasmidprep: 184.pSB1C3-B0034-CrtZ Transformation: 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 302. idi (mut)-B0034-Ispa 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 305.pSB1A3-j23110-B0034-CrtZ O/N screening: 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <h2>Results:</h2> Failed: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208.pSB1A3-B0034-Thioredoxin-ZCD-His 272 pSB1K3-B0034-Thioredoxin-ZCD-His 273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'

+

}

+

else if(id == 'd2013819')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1> Name of participants: Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Screening 134.1 pSB1C3-CBP 254 pSB1C3-gus 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.3 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.2 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI 254.2 pSB1C3-gus: XbaI & PstI 255.1 pSBLB4C15-pemp: EcoRI & PstI 255.2 pSBLB4C15-pemp: EcoRI & PstI 257.1 pSB1C3-pemp: EcoRI & PstI 257.2 pSB1C3-pemp: EcoRI & PstI Frozen stock: 134.1 pSB1C3-CBP 275. pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry 1 ml overnight culture: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 139. pSB3K3-J23101-B0034-BFP Plasmid preparation 111.7(1) pSBLB4C15-red 111.7(2) pSBLB4C15-red 134.1 pSB1C3-CBP 275 pSBLB4C15-red (dam-) 288.1 pSB1C3-pem 288.2 pSB1C3-pem 288.4 pSB1C3-pem 289.1 pSB1C3-mCherry 289.3 pSB1C3-mCherry 289.4 pSB1C3-mCherry Overnight culture: Lb11.2 plantarum 36E-no plasmid Phusion PCR: usp45 (synthesized signal peptide from Lactococcus lactis) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ digest 36. pEL3S17-red Ligation 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results:</h2> Screening 134.1 pSB1C3-CBP: showed the correct band! 254 pSB1C3-gus: showed a very weak band, not sure if correct! 288.1 pSB1C3-pem: showed the correct band! 288.2 pSB1C3-pem: showed the correct band! 288.3 pSB1C3-pem: did not show the correct band! 288.4 pSB1C3-pem: showed the correct band! 289.1 pSB1C3-mCherry: showed the correct band! 289.2 pSB1C3-mCherry: did not show the correct band! 289.3 pSB1C3-mCherry: showed the correct band! 289.4 pSB1C3-mCherry: showed the correct band. 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail Digestion & screening: 254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands! 255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands! 257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! 257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands! Plasmid preparation 111.7(1) pSBLB4C15-red: 81.7 ng/µl 111.7(2) pSBLB4C15-red: 93.9 ng/µl 134.1 pSB1C3-CBP: 163.8 ng/µl 275 pSBLB4C15-red (dam-): 92.4 ng/µl 288.1 pSB1C3-pem: 211.2 ng/µl 288.2 pSB1C3-pem: 193.2 ng/µl 288.4 pSB1C3-pem: 193.9 ng/µl 289.1 pSB1C3-mCherry: 230.4 ng/µl 289.3 pSB1C3-mCherry: 229.7 ng/µl 289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'

+

}

+

else if(id == 'd2013820')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1> Name of participants: Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Characterisation of the CP promoters with facs: 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP Digestion: USP. with E, P 191. pSB1A2-fusionGFP with X, P 280. pSB1A3-B0034-aeBlue with X, P 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin Making competent Lactobacillus reuteri Lb12.1 reuteri DSM 20016 plasmid free: eight Lb12.2 reuteri DSM 20016 plasmid free: eight Electroporation Lb3.2 reuteri DSM 20016 pVS2: two 111.7(1) pSBLB4C15-red: one 111.7(2) pSBLB4C15-red: two 255.1 pSBLB4C15-pemp: one 255.2 pSBLB4C15-pemp: one 275 pSBLB4C15-red (dam-): three <h2>Results:</h2> Electroporation Lb3.2 reuteri DSM 20016 pVS2: one of the two survived 111.7(1) pSBLB4C15-red: survived 111.7(2) pSBLB4C15-red: one of the two survived 255.1 pSBLB4C15-pemp: survived 255.2 pSBLB4C15-pemp: survived 275 pSBLB4C15-red (dam-): two of the three survived</div>'

+

}

+

else if(id == 'd2013821')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1> Name of participants: Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 204(1-4). pEL3S17-J23110-B0034-His-STS E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work: </h2> Digestion: usp45: XbaI & AgeI 191.1 pSB1A2-fusionGFP: XbaI, PstI 191.1 pSB1A2-fusionGFP: NgoMIV & PstI 275. pSBLB4C15-red (dam-): EcoRI & PstI 289.1 pSB1C3-mCherry: NgoMIV & PstI 289.1 pSB1C3-mCherry: XbaI, PstI 297.1 pSBLB4B15-red (dam-): EcoRI & PstI Ligation: pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1) pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1) pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1) pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1) pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1) pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1) pSB1C3-usp45 (1+usp45) pSB1C3-usp45 (6+usp45) pSBLb4C15-CP1-B0034-BFP pSBLb4C15-CP8-B0034-BFP pSBLb4C15-CP11-B0034-BFP pSBLb4C15-CP29-B0034-BFP pSBLb4C15-CP30-B0034-BFP pSBLb4C15-CP41-B0034-BFP pSBLb4C15-CP44-B0034-BFP pSBLb4C15-CP11-aeblue pSBLb4C15-CP30-aeblue pSBLb4B15-CP11-mcherry pSBLb4B15-CP30-mcherry pSBLb4B15-CP11-tspurple pSBLb4B15-CP30-tspurple pSBLb4B15-CP11-amilCP pSBLb4B15-CP30-amilCP pSBLb4B15-CP11-amilGFP pSBLb4B15-CP30-amilGFP 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Transformation: 108.pEL3S17-J23106-B0034-IspA 141.pRL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 272.pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ 204(1-4) Re-streak: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Screen/ ON: 219.pSB1C3-Miraculin 304.pSB1A3-J23106-B0034-CrtZ 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 280. pSB1A3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP Plasmid prep. 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His Mutagenesis PCR: 336. pSB1C3-spisPink 337. pSB1C3-scOrange 338. pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 257. pSB1C3-pemp 288. pSB1C3-pem <h2>Other experiments:</h2> Phosphorylation of primers (20µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R Cleaning of electroporation cuvettes</div>'

+

}

+

else if(id == 'd2013822')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1> Name of participants: Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus <h2>Ongoing constructs:</h2> E. coli: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 70. pSB3K3-CP11-B0034-BFP 71. pSB3K3-CP29-B0034-BFP 72. pSB3K3-CP30-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 74. pSB3K3-CP44-B0034-BFP 111. pSBLb4C15-red 134. pSB1C3-CBP 139. pSB3K3-J23101-B0034-BFP 233. pSBLb4C15-CP1-B0032-BFP 234. pSBLb4C15-CP8-B0032-BFP 235. pSBLb4C15-CP11-B0032-BFP 236. pSBLb4C15-CP29-B0032-BFP 237. pSBLb4C15-CP30-B0032-BFP 238. pSBLb4C15-CP41-B0032-BFP 239. pSBLb4C15-CP44-B0032-BFP 254. pSB1C3-gus 255. pSBLb4C15-pemp 256. pSBLb4C15-pem 257. pSB1C3-pemp 267 pSB1C3-pemp 288. pSB1C3-pem 289. pSB1C3-mCherry 290. pSB1C3-CBP 291. pSBLb4C15-CP1-mCherry 293. pSBLb4C15-CP8-mCherry 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His (201. E.Coli. Nissle - CrtY-CrtI) 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) E. coli PK401 dam-: 275.2 pSBLb4C15-red 276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 292. pSBLb4C15-CP1-mCherry 294. pSBLb4C15-CP8-mCherry <h2>Todays work:</h2> Competent cell preparation: Lb11.1 plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Lb12.2 reuteri DSM 20016 no plasmid Digest: 147.B0034-Thioredoxin (E, S) 147.B0034-Thioredoxin (X,P) 27.pEL3K16-red (E,P) 125.pSB1C3-ZCD-His (X,P) Ligation: 208. pSB1A3-B0034-Thioredoxin-ZCD-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin Transformation: 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP <strike>3.2. pVS2 (died in electroporation) 310. pSBLB4B15-CP11-fusionGFP (died in electroporation) 318. pSBLB4B15-CP11-mCherry (died in electroporation) 320. pSBLB4B15-CP11-amilGFP (died in electroporation) 313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation) 317. pSBLB4B15-CP30-tsPurple (died in electroporation) 331. pSBLB4C15-CP41-BFP (died in electroporation) 332. pSBLB4C15-CP44-BFP (died in electroporation)</strike> + other E. coli transformations Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Screen/ ON: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1A3-j23101-B0034-CrtZ 304.pSB1A3-j23106-B0034-CrtZ Plasmid prep. / Seq. prep. 147.pSB1C3-B0034-Thioredoxin 184.pSB1C3-B0034-CrtZ Gel: 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Phusion PCR: ->219.pSB1C3-Miraculin <h2>Results:</h2> Mutagenesis PCR: 257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do. 288. pSB1C3-pem: --”-- Transformation: 11.1 Lb. plantarum 36E: 3.2 pVS2: survived electroporation 3.2 pVS2: died during electroporation 310. pSBLB4B15-CP11-fusionGFP: died during electroporation 311. pSBLB4B15-CP30-fusionGFP: survived electroporation 312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation 313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation 316. pSBLB4B15-CP11-tsPurple: survived electroporation 317. pSBLB4B15-CP30-tsPurple: died during electroporation 12.2 Lb. reuteri DSM 20016: 3.2 pVS2: survived electroporation 318. pSBLB4B15-CP11-mCherry: died during electroporation 319. pSBLB4B15-CP30-mCherry: survived electroporation 320. pSBLB4B15-CP11-amilGFP: died during electroporation 321. pSBLB4B15-CP30-amilGFP: survived electroporation 322. pSBLB4B15-CP11-amilCP: survived electroporation 323. pSBLB4B15-CP30-amilCP: survived electroporation 324. pSBLB4C15-CP11-aeBlue: survived electroporation 12.1 Lb. reuteri DSM 20016: 325. pSBLB4C15-CP30-aeBlue: survived electroporation 326. pSBLB4C15-CP1-BFP: survived electroporation 327. pSBLB4C15-CP8-BFP: survived electroporation 328. pSBLB4C15-CP11-BFP: survived electroporation 329. pSBLB4C15-CP29-BFP: survived electroporation 330. pSBLB4C15-CP30-BFP: survived electroporation 331. pSBLB4C15-CP41-BFP: died during electroporation 332. pSBLB4C15-CP44-BFP: died during electroporation <h2>Characterization:</h2> Western blot analysis (SDS-page) on constructs: 115. pSB3K5-J23101-B0034-His-TAL 116. pSB3K3-J23110-B0034-His-TAL 117. pSB3K3-CP1-B0034-His-TAL 119. pSB1C3-B0034-4CL (from 15) (neg.contr) <h2>Other experiments:</h2> Phosphorylation of primers (60 µl of each): Pem-mutE_F Pem-mutE_R Pem-mutS_F Pem-mutS_R</div>'

+

}

+

else if(id == 'd2013823')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1> Name of participants: Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> E. coli (D5α): 111.7 pSBLb4C15-red 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Gel electrophoresis: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R 111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites) Mutagenesis PCR: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R Touchdown with Tm1+2 and Tm1+4. Transformation: 102.pSB1C3-CrtE 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208. pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 300. pEL3S17-J23106-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin Re-streak: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Screening O/N: 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 222.pSB3K3-CP1-B0034-UGTCs2-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin O/N: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Glycerol stock Plasmidprep/sekvensprep: 108.pEL3S17-J23106-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 184.pSB1C3-B0034-CrtZ 299.pEL3S17-J23101-B0034-Thioredoxin <h2>Results:</h2> Gel electrophoresis: 257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!* 111.7 pSBLb4C15-red (cut with E and P): No band *→ redo mutagenesis PCR with higher temperatures</div>'

+

}

+

else if(id == 'd2013824')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1> Name of participants: Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M <h2>Ongoing constructs:</h2> Escherichia coli 257. pSB1C3-pemp 288. pSB1C3-pem 136. pSB1C3-B0034-USP pSB1C3-B0034-usp45 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS Lactobacillus reuteri: Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP Lactobacillus plantarum: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Digest: 200.pSB1C3-B0034-asCP E,X 148.pSB1C3-RBS-gfasPurple E,X 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI) Ligation: 200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→ → 343.J23106-B0034-asCP 148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→ → 160.j23106-RBS-gfasPurple 148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→ → 159j23110-RBS-gfasPurple 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→ →215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His Transformation: 223.pSB3K3-CP8-B0034-UGTCs2-His 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 343.pSB1C3-J23106-B0034-asCP 160.pSB1C3-j23106-RBS-gfasPurple 159.pSB1C3-j23110-RBS-gfasPurple 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 336.pSB1C3.spisPink 337.pSB1C3.scOrange 284.pSB1C3.B0034-aeBlue 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 284. pSB1C3-B0034-aeBlue 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP O/N screening: 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 136. 312 313 315 321 325 327 332 317 O/N: 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Plasmid prepp & sequence prepp 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink Gel electrophoresis: Lb31. plantarum 36E pVS2 Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple Lb35. reuteri DSM 20016 pVS2 Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R + E. coli transformations PCR-purification: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R DpnI: 257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R 288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R <h2>Results:</h2> Transformations: All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. Gel electrophoresis: All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite. The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'

+

}

+

else if(id == 'd2013825')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1> Name of participants: Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Digest: 147.pSB1C3-B0034-Thioredoxin Ligate: 147.pSB1C3-B0034-Thioredoxin+CP1→ → 346.pSB1C3-CP1-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP8→ →347.pSB1C3-CP8-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP41→ →348.pSB1C3-CP41-B0034-Thioredoxin Transformation: 1.pSB1C3-Red (competens test) 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ 336.pSB1C3-spisPink 337pSB1C3-scOrange 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348.pSB1C3-CP41-B0034-Thioredoxin 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple Re-streak: 284. pSB1C3-B0034-aeBlue Sequenceprep: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) </div>'

+

}

+

else if(id == 'd2013826')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1> Name of participants: Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin <h2>Todays work</h2> Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 139. pSB3K3-J23101-B0034-BFP PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Slovenian 4CL with His-primers Gel electrophoresis: Erythromycin-cassette PCR-product from Lb2.1 pLUL631 Erythromycin-cassette PCR-product from Lb2.2 pLUL631 Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R Assembly & transformation: 349 Mutagenesis 338.pSB1C3-meffBlue Re streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP Plasmidprepp: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin O/N screen 284. pSB1C3-B0034-aeBlue Screening: 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348. pSB1C3-CP41-B0034-Thioredoxin O/N: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades) Re-streak 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades) Sekvensprep: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation: 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → →215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Results:</h2> Gel electrophoresis: Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly Erythromycin-cassette PCR-product: --”-- Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed 77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp. 269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made. <h2>Characterization:</h2> Western blot sample preparation: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparing MRS-agar Preparing low salt LB-agar</div>'

+

}

+

else if(id == 'd2013827')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-27</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> O/N: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 Transformation: Chromoproteins into pSB3K3 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1K3-CP1-B0034-Thioredoxin 347.pSB1K3-CP8-B0034-Thioredoxin 348.pSB1K3-CP41-B0034-Thioredoxin Gel electrophoresis: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Screening PCR: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ Sekvensprep: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 284. pSB1C3-B0034-aeBlue Re-streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures Tm1 and +5°C (with touchdown)) Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml 100 µg/ml <h2>Results</h2> Gel electrophoresis: 77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp 77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp <h2>Other experiments</h2> Preparing test plates, to determine minimum antibiotic concentration: Lb-erythromycin: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml MRS-zeocin: 50 µg/ml 100 µg/ml</div>'

+

}

+

else if(id == 'd2013828')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-08-28</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 58. pSB1C3- B0034-IspA 62.pSB1C3-CP8 95. pSB1C3-K206001 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 129.pSB1C3-Idi(mut) 140.pSB3T5-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ 193.pSB1C3-amilGFP 194.pSB1C3-B0034-amilCP 195.pSB1C3-cjBlue 196.pSB1C3-eforRed 197.pSB1C3-aeBlue 198.pSB1C3-B0034-amilGFP 199.pSB1C3-amajCFP 200.pSB1C3-B0034-asCP 201. E.Coli. Nissle - CrtY-CrtI 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 219.pSB1C3-Miraculin 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit) 227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit) 228. pEL3A17-Blue 229. pEL3K17-Blue 230. pEL3S15-Blue 231. pEL3C17-Blue 253. pEL3S17-J23101-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His 282.pSB3K3-J23110-B0034-amilCP 283.pSB3K3-J23110-B0034-asCCP 299.pEL3S17-J23101-B0034-Thioredoxin 300.pEL3S17-J23106-B0034-Thioredoxin 301.pEL3S17-J23110-B0034-Thioredoxin <h2>Todays work</h2> Gel electrophoresis: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 77. pSB4C15-red (annealing at Tm1): 77. pSB4C15-red (annealing at Tm1+5): Ligation: 352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI)) 108.pEL3S17-J23106-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-His 141.pEL3S17-J23101-B0034-UGTCs2-His 142.pEL3S17-CP1-B0034-UGTCs2-His 143.pEL3S17-CP8-B0034-UGTCs2-His PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1) Restreak 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ Screening O/N: 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 147.B0034-Thioredoxin 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 267.psB3K3-K206000-B0034-IspA 268.psB3K3-K206001-B0034-IspA 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 300.J23106-B0034-Thioredoxin 202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 Digest 36. pEL3S17-red Subclone: 280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue 281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue 282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP 283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP Two step Assembly: 92. BBa_J61002-J23110, 149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed 91. BBa_J61002-J23106, 149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed 92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink 91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink 92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple 91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml: Growth 10 µg/ml: Growth 20 µg/ml: Growth 50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency. - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml: Growth 100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.) - lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates: Everything grew on Ab-, plantarum slightly less. Gel electrophoresis: 351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp 351.2 pSB1C3-pemp-mut1: Nothing 77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1. 77. pSB4C15-red (annealing at Tm1+5): Nothing. 202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed 203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed 207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed Re-streak: 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <h2>Other experiments</h2> Preparation of new test-plates: LB-erythromycin: 100 µg/ml 200 µg/ml</div>'

+

}

+

else if(id == 'd2013829')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-08-29</h1> Name of participants: Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> E. coli: 353. pSBLb4E15 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His <h2>Todays work</h2> Transformation: 353. pSBLb4E15 Plasmidprep: 223 346 <h2>Results</h2> Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 100 µg/ml: Weak growth 200 µg/ml: Possibly no growth, let them “grow” some more. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparation of new test-plates: MRS-Zeocin: 250 µg/ml </div>'

+

}

+

else if(id == 'd2013830')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-08-30</h1> Name of participants: Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 77. pSB4C15-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Lb9. faecalis JH2-2 pAMbeta <h2>Todays work</h2> Gel extraction: 77. pSB4C15-red (annealing at Tm1): PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R* *Since the product from monday disappeared. PCR purification: Lb9. faecalis JH2-2 pAMbeta1-ori Digestion: Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII) 77. pSB4C15-red (PCR product) (NheI and BglII) Ligation: 169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon) <h2>Results</h2> Western blot: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd2013831')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-08-31</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) 386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1) Gel electrophoresis: 169. pSBLbEcC2-red (ligation) Ligation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 352. pSBLbC3-red (pEL+ replicon from pVS2) <h2>Results</h2> Gel electrophoresis: 169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'

+

}

+

else if(id == 'd201391')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-01</h1> Name of participants: Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red 406. pSBLbC3-red (dam-) 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> Transformation: 111. pSBLb4C15-red (pEL+ replicon from pJP) 275. pSBLb4C15-red (dam-) 352. pSBLbC3-red (pEL+ replicon from pVS2) 406. pSBLbC3-red (dam-) Screening: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2) Re-streak: 169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) <h2>Results</h2> First screening failed (weird gel) <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201392')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-02</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> O/N: 169. pSBLbEcC2-red Mutagenesis PCR: 111.7 pSBLb4C15-red * *with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night. Ligation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* 352. pSBLbC3-red* *New attempts at ligation, just in case. <h2>Results: </h2> Gel electrophoresis: 111.7 pSBLb4C15-red: No bands Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS failed. <h2>Characterization experiments:</h2> Western blot on construct: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201393')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1> Name of participants: Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 261. pSB3K3-J23110-B0034-His-4CL 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red Gel electrophoresis: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) Transformation: 111. pSBLb4C15-red (pEL4C15+replicon from pJP059)* *New attempts at ligation, just in case. Sequencing preparation: 169. pSBLbEcC2-red <h2>Characterization experiments:</h2> Western blot sample preparation on construct: 335. pSB3K3-J23101-HIs-STS <h2>Results: </h2> Frozen stock and plasmid preparation: 169. pSBLbEcC2-red: 44.2 ng/µl 169. pSBLbEcC2-red: 47.8 ng/µl Gel electrophoresis: 111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'

+

}

+

else if(id == 'd201394')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1> Name of participants: Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs: </h2> 111. pSBLb4C15-red 169. pSBLbEcC2-red 352. pSBLbC3-red 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> Assembly: 353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old) Trasformation: 111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP) 353. pSBLb4E15-red PCR-purification: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried) DpnI-treatment: 111.7 pSBLb4C15-red* *to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'

+

}

+

else if(id == 'd201395')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1> Name of participants: Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs:</h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work</h2>First day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201396')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1> Name of participants: Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L <h2>Ongoing constructs: </h2> 335. pSB3K3-J23101-HIs-STS <h2>Todays work: </h2> PCR-purification and recircularization: Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30) O/N: 111.7 pSBLb4C15-red* *to do a new plasmid preparation since old may have corrupt red-cassette/contamination) Streak from frozen stock: 111.7 pSBLb4C15-red* * to test if red-cassette has been damaged, or possibly the stock contaminated. Second day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Characterization:</h2> Western blot on construct: 335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201397')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1> Name of participants: Mikael Strandgren, Christoffer L <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work:</h2> Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE) 355.2 pSB3K3-CP29-mCherry 355.3 pSB3K3-CP29-mCherry 357.3 pSB3K3-CP30-usp45-mCherry 357.4 pSB3K3-CP30-usp45-mCherry 358.1 pSB3K3-CP29-usp45-mCherry 358.2 pSB3K3-CP29-usp45-mCherry 363. pSB3K3-CP30-tspurple 364. pSB3K3-CP29-tspurple 365. pSB3K3-CP11-tspurple 366. pSB3K3-CP30-amilCP 367. pSB3K3-CP29-amilCP 368. pSB3K3- CP11-amilCP 369. pSB3K3-CP30-aeblue 370. pSB3K3-CP29-aeblue 371. pSB3K3-CP11-aeblue Assembly: 407. pSB1C3-CP29-Mcherry* 408. pSB1C3-CP30-usp45-mCherry* 409. pSB1C3-CP29-usp45-mCherry* 410. pSB1C3-CP30-tsPurple* 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP* 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue* 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old) * pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today. Transformation 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette. O/N: 111.7 pSBLb4C15-red Third day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. <h2>Results: </h2> O/N: 111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today. Plasmid preparation: 351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl 355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl 355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl 357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl 357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl 358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl 358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl 363. pSB3K3-CP30-tspurple: 75.5 ng/µl 364. pSB3K3-CP29-tspurple: 43.9 ng/µl 365. pSB3K3-CP11-tspurple: 47.3 ng/µl 366. pSB3K3-CP30-amilCP: 74.5 ng/µl 367. pSB3K3-CP29-amilCP: 77.2 ng/µl 368. pSB3K3- CP11-amilCP: 34.3 ng/µl 369. pSB3K3-CP30-aeblue: 37.4 ng/µl 370. pSB3K3-CP29-aeblue: 60.3 ng/µl 371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'

+

}

+

else if(id == 'd201398')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-08</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs: </h2> 407. pSB1C3-CP29-Mcherry 408. pSB1C3-CP30-usp45-mCherry 409. pSB1C3-CP29-usp45-mCherry 410. pSB1C3-CP30-tsPurple 411. pSB1C3-CP29-tsPurple 412. pSB1C3-CP11-tsPurple 413. pSB1C3-CP30-amilCP 414. pSB1C3-CP29-amilCP 415. pSB1C3-CP11-amilCP 416. pSB1C3-CP30-aeBlue 417. pSB1C3-CP29-aeBlue 418. pSB1C3-CP11-aeBlue 419. pSBLbEcE-red <h2>Todays work: </h2> Transformations: 411. pSB1C3-CP29-tsPurple * 412. pSB1C3-CP11-tsPurple * 414. pSB1C3-CP29-amilCP * 415. pSB1C3-CP11-amilCP * 417. pSB1C3-CP29-aeBlue * 418. pSB1C3-CP11-aeBlue * * The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good. ON: 353. pSBLB4E15-red <h2>Results: </h2> Transformation 411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today. 412. pSB1C3-CP11-tsPurple: “ 414. pSB1C3-CP29-amilCP “ 415. pSB1C3-CP11-amilCP “ 417. pSB1C3-CP29-aeBlue “ 418. pSB1C3-CP11-aeBlue “ 419. pSBLbEcE-red: Growth covering the plates, only white colonies. pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'

+

}

+

else if(id == 'd201399')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Digest 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation 338.pSB1C3-meffBlue 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Fourth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013910')

+

{

+

ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1> Name of participants: Sabri, Christoffer L <h2>Todays Work:</h2> Re-streak 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Fifth day of survival test: O/N in antibiotic-free medium and re-streak on plates with and without antibiotics. </div>'

+

}

+

else if(id == 'd2013911')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1> Name of participants: Sabri <h2>Todays Work:</h2> O/N: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd2013912')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1> Name of participants: Sabri <h2>Todays Work:</h2> Plasmid prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue Sequence prep: 339. pSB1C3-gfasPurple 340. pSB1C3-AmajCP(AmajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd20131013')

+

{

+

ds ='<h1>European Jamboree Lyon end</h1>';

+

}

+

else if(id == 'd20131012')

+

{

+

ds ='<h1>European Jamboree Lyon</h1>';

+

}

+

else if(id == 'd20131011')

+

{

+

ds ='<h1>European Jamboree Lyon start</h1>';

+

}

+

else if(id == 'd2013104')

+

{

+

ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';

+

}

+

else if(id == 'd2013102')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Sequencing preparation: Lb47. reuteri 100-23 pSBLbE-red </div>'

+

}

+

else if(id == 'd2013101')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1> Name of participants: Anders Edlund <h2>Ongoing constructs </h2> Lb47. reuteri 100-23 pSBLbE-red <h2>Todays work</h2> Screening PCR: Lb47. reuteri 100-23 pSBLbE-red <h2>Results</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2: Growing Lb13. pJP059: Growing 420. pSBLbE-CP11-amilCP: Not growing 435. pSBLbC-CP29-amilCP: Not growing 440. pSBLbC-red: Not growing 441. pSBLbE-red: Growing All negative controls were clear, and the positive controls grew. </div>'

+

}

+

else if(id == 'd2013928')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1> Name of participants: Viktor Blomkvist, Mikael Strandgren, Erik Edlund <h2>Ongoing constructs</h2> E. coli: 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red L. reuteri: Lb3. pVS2 Lc. lactis: Lb13. pJP059 <h2>Todays work</h2> Transformation: To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 Lb13. pJP059 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013927')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Overnight: Lb4. reuteri 100-23 no plasmid Lb11. Plantarum 256 no plasmid Lb12. Reuteri DSM20016 no plasmid Streak from frozen stock (for photos): 420. pSBLbE-CP11-amilCP 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red </div>'

+

}

+

else if(id == 'd2013924')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> <h2>Results</h2> Transformation: No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday. </div>'

+

}

+

else if(id == 'd2013921')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1> Name of participants: Erik Edlund, Mikael Strandgren, Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Todays work</h2> Transformation To L. reuteri DSM 20016 and L. plantarum 256: Lb3. pVS2 (positive control) 435. pSBLbC-CP29-amilCP 440. pSBLbC-red 441. pSBLbE-red <h2>Other experiments</h2> Making MRS-agar plates: Erythromycin Chloramphenicol Antibiotic-free </div>'

+

}

+

else if(id == 'd2013920')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1> Name of participants: Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Plasmid preparation: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013919')

+

{

+

ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';

+

}

+

else if(id == 'd2013918')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1> Name of participants: Viktor Blomkvist <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> O/N: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013917')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP <h2>Todays work</h2> Re-streak: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else if(id == 'd2013916')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1> Name of participants: Mikael Strandgren, Anders Edlund <h2>Ongoing constructs</h2> 435. pSBLbC-CP29-amilCP 440. pSBLbC-red <h2>Todays work</h2> Plasmid preparation: 440. pSBLbC-red Assembly and transformation: 435. pSBLbC-CP29-amilCP </div>'

+

}

+

else

+

{

+

ds = 'Nothing happend during this day!';

+

}

+

document.getElementById(id).setAttribute('onclick','closeNote('+y+', '+m+', '+d+');');

+

document.getElementById(id).innerHTML = ds;

+

}

+

function closeNote(y, m, d)

+

{

+

var ds = '';

+

var id = 'd'+y+m+d;

+

var ds = '<h1>'+y+'-'+m+'-'+d+'</h1>';

+

document.getElementById(id).setAttribute('onclick','diry('+y+', '+m+', '+d+');');

+

document.getElementById(id).innerHTML = ds;

+

}

</script>

+

</html>

@@ Line 1: / Line 1: @@
+<html>
 <script type="text/javascript">
-var month = new Date().getMonth();
+var month = 8;
 var year = new Date().getFullYear();
 var cal_table = '';
+var eventString;
 var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
+var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');
+var inputs = 106;
 Date.prototype.getWeek = function()
@@ Line 17: / Line 21: @@
    var today = date.getDate();
    cal_table = '';
+  var eventString;
     if(year<=200)
@@ Line 26: / Line 31: @@
     days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
-     if(year%4 == 0 && year!=1900)
+ if(year%4 == 0)
    {
-      days_in_month[1]=29;
+   if( year!=1900 )
-     }
+   {
+   days_in_month[1]=29;
+   }
+  }
      total = days_in_month[month];
      var date_today = months[month]+' '+year;
-     cal_table = cal_table + '<table class="cal_calendar" ><tbody id="cal_body"><tr class="head"><th class="arrow"><input id="left" type="image" onclick="left_arrow()" src="image/buttons/arrowleft.png"></th><th colspan="6" >'+date_today+'</th><th class="arrow"><input id="right" type="image" onclick="right_arrow()" src="image/buttons/arrowright.png"></th></tr>';
+     cal_table = cal_table + '<table class="cal_calendar" ><tbody id="cal_body"><tr class="head"><th class="arrow"> <input id="left" type="image" onclick="left_arrow()" src="https://static.igem.org/mediawiki/2013/a/a1/Arrowleft.png"></th><th colspan="6" >'+date_today+'</th><th class="arrow"><input id="right" type="image" onclick="right_arrow()" src="https://static.igem.org/mediawiki/2013/0/0b/Arrowright.png"></th></tr>';
      cal_table = cal_table + '<tr class="cal_d_weeks"><th>Mon</th><th>Tue</th><th>Wed</th><th>Thu</th><th>Fri</th><th>Sat</th><th>Sun</th><th>Week</th></tr><tr>';
      week = 0;
@@ Line 53: / Line 61: @@
          }
-        if(today==i && month == date.getMonth() && year == date.getFullYear())
+                if(year == date.getFullYear())
          {
-            cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="cal_today"><input type="submit" onclick="" value="'+i+'"></td>';
+          if(month == date.getMonth()){
+             if(today==i){
+             cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="cal_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
+          }
+         else{
+         cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
+        }
+        }
+        else{
+        cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
+        }
          }
          else
          {
-             cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="" value="'+i+'"></td>';
+             cal_table = cal_table +'<td id="'+'t'+year+m+i+'" class="not_today"><input type="submit" onclick="showNotes(year, month, value)" value="'+i+'"></td>';
          }
-        week++;
+              week++;
+  markerNotes(year, m, i);
          if(week==7)
          {
    var weeknr = new Date(year, month, i-1).getWeek();
-   cal_table = cal_table + '<td class="week"><input type="submit" onclick="" value="'+weeknr+'"></td>';
+   cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';
          cal_table = cal_table + '</tr>';
    week=0;
@@ Line 89: / Line 108: @@
        var weeknr = weeknr+1;
        }
-       cal_table = cal_table + '<td class="week"><input type="submit" onclick="" value="'+weeknr+'"></td>';
+       cal_table = cal_table + '<td class="week"><input type="submit" onclick="showNotesOfweek(year, value)" value="'+weeknr+'"></td>';
                          cal_table = cal_table + '</tr>';
                          week=0;
@@ Line 96: / Line 115: @@
          cal_table = cal_table + '</tbody></table>';
   }
+function left_arrow()
+ {
+ month = month-1;
+  if(month == -1)
+  {
+   year = year-1;
+   month = 11;
+  }
+ calendar();
+ document.getElementById('calendar').innerHTML = cal_table;
+ }
+ function right_arrow()
+ {
+ month = month+1;
+  if(month == 12)
+  {
+   year = year+1;
+   month = 0;
+  }
+ calendar();
+ document.getElementById('calendar').innerHTML = cal_table;
+ }
+ function showNotes(y, m, day)
+ {
+  var m = month+1;
+  eventString =  '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+  document.getElementById('file_holder').innerHTML = eventString;
+document.getElementById('calendar').style.marginBottom="350px";
+ }
+function showNotesOfweek(y, wnr)
+ {
+  var d = new Date(y, 0, 0);
+  if(y == 2012 ){
+		wnr++;
+	}
+  var w = d.getTime() + 604800000 * (wnr-1);
+  var m = new Date(w).getMonth()+1;
+  var y = new Date(w).getFullYear();
+  var day = new Date(w).getDate();
+   var e = new Date(w).getDay();
+   day = day - e+1;
+  eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+   for(i=1;i<7;i++)
+   {
+    if(days_in_month[m-1] == day )
+    {
+     day = 0;
+     m++;
+     if(m>12)
+     {
+      y++;
+      var d = new Date(y, 0, 0);
+      var w = d.getTime() + 604800000 * (wnr-1);
+      var m = 1;
+     }
+    }
+    day++;
+    eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
+   }
+document.getElementById('calendar').style.marginBottom="350px";
+  document.getElementById('file_holder').innerHTML = eventString;
+  eventString = '';
+ }
+ function markerNotes(y, m, d)
+ {
+ var id='t'+y+m+d;
+ for(c = 0; c<=inputs; c++)
+ {
+  if(days_with_dirys[c] == id)
+  {
+   cal_table = cal_table + '<style type="text/css">#'+id+'{border: 2px solid #0089d1;}</style>';
+  }
+ }
+}
+function diry(y, m, d)
+{
+ var ds = '';   //diryString
+ var id = 'd'+y+m+d;
+ //var ds = y+'-'+m+'-'+d;
+ if(id == 'd2013630')
+ {
+ ds = '<div id="dairy-text"><h1>Sunday 2013-06-30</h1><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Mikael S, Anders E<br><br><h2>Ongoing constructs:</h2><b>E. coli:</b><br>75. pEL3A15 - CrtB<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51.pEL3C18-red<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><b>L. reuteri:</b><br>Lb1. CF48-pLR581<br>Lb2. 1063-pLUL631<br>Lb3. DSM 20016-pVs2<br>Lb4. 100-23-noplasmid<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br>Lb8. 100-23-pGT232<br>Lb12. DSM 20016-noplasmid<br>Lb14. DSM 20016-pLUL631<br><br><b>L. plantarum:</b><br>Lb5. 256-rifR-pAMβ1<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br>Lb16. MG1363-noplasmid<br><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br>Lb9. JH2-2 pAMβ1<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>75. pEL3A15 - CrtB<br><br><b>Digest</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br><br><b>Ligation</b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>We made a transformation with one sample of 77 from today, and one from yesterday to try to see if the problem yesterday laid with the transformation or the assembly.<br><br><b>O/N Screening</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results</h2><br><b>Re-streak</b><br>Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br></div>';
+ }
+ else if(id == 'd2013629')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Anders E, Mikael S<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli (strain D5α):</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Todays work</h2><b>PCR purification:</b><br>CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br><b>Digestion:</b><br>27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br><b>Ligation:</b><br>24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br><b>Transformation:</b><br>75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br><b>Re-streak:</b><br>1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br><b>Plasmid preparation:</b><br>42. pSB4S15-red<br><br><b>O/N Screening PCR:</b><br>25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>(19,20,21(with DMSO dilution series(7,9,12))<br><br><b>PCR (screening?):</b><br>57.B0034-Idi<br>IspA<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br><h2>Results</h2><b>Today:</b><br><b>Plasmid preparation:</b><br>42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br><b>Transformation:</b><br>75. pEL3A15 - CrtB failed<br><br><b>PCR purification (of promotors):*</b><br>CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor constructs (not 77) from annealing of R and F (without PCR-cleaning).<br><br><b>Previous day:</b><br><b>ON screening:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></div>';
+ }
+else if(id == 'd2013628')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><b>Name of participants: </b>Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br><h2>Ongoing constructs:</h2><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br><h2>Todays work</h2><b>Frozen stock:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis MG1363 noplasmid clone 2<br><br><b>Digest:</b><br>25.pSB1C3-CrtY<br><br><b>Transformation:</b><br>24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Re-streaks:</b><br> Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br><b>Streaking of strains:</b><br> Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br><b>O/N of restreak from Erik Gullberg:</b><br>42. pSB4S15-red, clones 1 and 2<br><br><b>PCR:</b><br>57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>** Now with DMSO<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N Screening:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br><br><h2>Results</h2><b>Previous day:</b><br><b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again with new protocol <br><br><b>Transformation:</b><br> Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br><b>Wednesday 26/6:</b><br><b>Streaking of strains:</b><br> Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br><br><b>Today:</b><br> <b>Plasmid preparation:</b><br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br><b>Gel electrophoresis:</b><br>57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br><h2>Other experiments</h2><b>THMS buffer preparation</b><br><b>Aliquoting of sigma water</b><br><b>Preparation of antibiotic-free LB-agar plates</b><br><b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>CP1_F<br>CP1_R<br>CP11_F<br>CP11_R <br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F <br>CP30_R <br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br><b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>usp45_mut_F<br><br><b>Phosphorylation of promotor-oligonucleotides:</b><br>CP1_F <br>CP1_R <br>CP11_F <br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>CP29_F<br>CP29_R<br>CP41_F<br>CP41_R</div>';
+ }
+else if(id == 'd2013627')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1><br><b>Name of participants: </b>Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>Lb11. plantarum 36E pVS2<br>Lb10. plantarum 256 pLUL634<br>Lb12. plantarum DSM20016 pVS2<br><br><b>Assembly:</b><br>23.Crt.B + pEL3A15<br>24.Crt.I + pEL3K16<br><br><b>Frozen stock: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>Lb13. Lc. lactis MG1363 pJP059 clone 1<br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb16. Lc. lactis MG1363 no plasmid clone 1<br><br><b>Plasmid preparation: </b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1 <br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Over night:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19. (digest from previous day)<br>20. (“)<br>21. (“)<br><br><b>PCR amplification of construct:</b><br>miraculin<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25. pSB1C3-CrtY<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N screening PCR:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25.pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * <br>Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! *<br>Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!*<br>Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>* Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day <br>** → Frozen stock and plasmid prep.<br><br><b>Transformation: </b><br>42. pSB4S15-red: Many small white colonies, contamination with cocci?<br>→ Discard, get clones from Erik G.<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059: Some growth.<br>Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock.<br>Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock.<br>→ let grow one more day.<br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl<br>Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl<br>Lb13. Lc. lactis  MG1363-pJP059 clone 1: 4.9 ng/µl (run 1)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3)<br>Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1)<br>→ Measure again tomorrow<br><br><b>Gel electrophoresis:</b><br>Miraculin: fail, problems with primers, probably due to problematic secondary structure.<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27)<br>19. (digest from previous day): Success<br>20. (“): Success<br>21. (“): Success<br>19. (PCR): Fail -> redo with lower TM<br>20. (“): Fail -> redo with lower TM<br>21. (“): Fail -> redo with lower TM<br>25. pSB1C3-CrtY: Good results<br><br><h2>Other experiments</h2><br><b>Preparation of THMS buffer</b></div>'
+ }
+else if(id == 'd2013626')
+ {
+	ds = '<div id="dairy-text"><h2>Wednesday 2013-06-26</h2><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Anders E, Mikael S, Anton B, Viktor B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Theodor L, Magnus B., Malin B., Thorsteinn O., Nils A., Alexander W., Niclas S., Pontus D., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>25.pSB1C3-CrtY<br>19.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.psB4C5-CrtE~I / w. linker, ZF & RBS<br>42. D5α with plasmid pSB4S15-red<br><br><h2>Todays work</h2><br><b>Competent cells preparation:</b><br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Transformation:</b><br>plantarum 36E pAMB1<br>plantarum 256 pAMB1<br>Lb3. reuteri DSM 20016 pVS2<br>25.pSB1C3-CrtY<br>42. pSB4S15-red <br><br><b>O/N:</b><br>Lb5.2 plantarum 256 pAMβ1*<br>Lb8.2 reuteri 100-23 pGT232*<br>Lb13. Lc. lactis MG1363 pJP059**<br>Lb15. Lc. lactis MG1363 pGus**<br>Lb16. Lc. lactis MG1363 no plasmid**<br>*Two tubes of 8.2 were prepared. It has grown rather poorly to not at all before. If it continues to grow poorly we may have to redo O/N from a plate rather than from our frozen stock.<br>** Optimized conditions → 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved.  <br><br><b>Digestion:</b><br>19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.1.psB4C5-CrtE~I / w. linker, ZF & RBS<br>21.2.psB4C5-CrtE~I / w. linker, ZF & RBS<br><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br><br><b>PCR amplification of construct:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (third try, using same concentration of template as second try but lower annealing temperature )<br>19.1.psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>19.2psB4C5-CrtE~O-Z / w. linker, ZF & RBS<br>20.1.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>20.2.psB4C5-CrtE~Z / w. linker, ZF & RBS<br>21.1.psB4C5-CrtE~I / w. linker, ZF & RBS<br>21.2.psB4C5-CrtE~I / w. linker, ZF & RBS<br><br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059: Growing, visual pellet.*<br>Lb15. Lc. lactis MG1363 pGus: Growing, visual pellet.*<br>Lb16. Lc. lactis MG1363 no plasmid: Growing, visual pellet.*<br>*Weak growth in the morning, better in the afternoon. → Let grow for one more day + new O/N with optimized conditions.<br><br><b>Streaking of strains: </b><br>Lb13. Lc. lactis MG1363 pJP059: No visual growth → Let grow for one more day.<br>Lb15. Lc. lactis MG1363 pGus: No visual growth →		--”--<br>Lb16. Lc. lactis MG1363 no plasmid: No visual growth → 	--”—<br><br><b>Today:</b><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: Failed, possibly nonspecific primer binding, not enough template DNA. Image 1 (2013.06.26)<br><br><b>Transformation:</b><br>25.pSB1C3-CrtY: failed<br>D5alpha competent cells were competent<br><br><br><h2>Other experiments</h2><br><b>Preparation of cold electroporation buffer for competent cells</b><br><br><b>Preparation of 50% glycerol stock: </b><br>500 ml 50% glycerol stock was prepared from 86-88% glycerol.</div>'
+ }
+ else if(id == 'd2013625')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1><br><br><b>Name of participants:</b> Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Gel electrophoresis:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>PCR amplification of construct:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature )<br>Lb13. Lc. lactis MG1363 pJP059<br><br><b>Competent cells preparation: </b><br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 noplasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Plasmid preparation: </b><br>Lb6.1 reuteri DSM 20016 pLUL634TsA8<br>Lb6.2 reuteri DSM 20016 pLUL634TsA8<br>Lb6.3 reuteri DSM 20016 pLUL634TsA8<br>Lb6.4 reuteri DSM 20016 pLUL634TsA8 *<br>Lb7.2 reuteri DSM 20016 pGFP<br>Lb7.3 reuteri DSM 20016 pGFP<br>Lb7.4 reuteri DSM 20016 pGFP *<br>Lb14.1 reuteri DSM 20016 pLUL631<br>Lb14.2 reuteri DSM 20016 pLUL631<br>Lb14.3 reuteri DSM 20016 pLUL631<br>Lb14.4 reuteri DSM 20016 pLUL631 *<br>* 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml.<br><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>PCR amplification: </b><br>Lb13. Lc. lactis MG1363 pJP059*<br>*Another attempt at colony PCR with different concentrations of lysozyme and buffer.<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25)<br>Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture.<br><br><b>Competant cells:</b><br>Wrong volume was added. → Try again tomorrow with the right volume.<br><br><b>Plasmid preparation: *</b><br>Lb6.1 reuteri DSM 20016 pLUL634 TsA8:	203.7 ng/µl<br>Lb6.2 reuteri DSM 20016 pLUL634 TsA8:	203.2 ng/µl<br>Lb6.3 reuteri DSM 20016 pLUL634 TsA8:	154.8 ng/µl<br>Lb6.4 reuteri DSM 20016 pLUL634 TsA8:	39.5 ng/µl<br>Lb7.2 reuteri DSM 20016 pGFP:		100.5 ng/µl<br>Lb7.3 reuteri DSM 20016 pGFP:		141.2 ng/µl<br>Lb7.4 reuteri DSM 20016 pGFP:		8.7 ng/µl<br>Lb14.1 reuteri DSM 20016 pLUL631:	162.7 ng/µl<br>Lb14.2 reuteri DSM 20016 pLUL631:	85.2 ng/µl<br>Lb14.3 reuteri DSM 20016 pLUL631:	49.8 ng/µl<br>Lb14.4 reuteri DSM 20016 pLUL631:	7.7 ng/µl<br>*The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones.<br><br><h2>Other experiments</h2><br><b>Preparation of MRS broth </b><br><br><b>Making plates for Lc. lactis cultivation (from M17-broth)</b><br><br><b>Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</b></div>'
+ }
+ else if(id == 'd2013624')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1><br><b>Name of participants: </b>Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D.<br><br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m	<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>Lb8. reuteri 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Digestion of construct:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>O/N (from frozen stock): </b><br>Lb2. 1063 pLUL631<br>Lb6. DSM 20016 pLUL63TsA8<br>Lb7. DSM 20016 pGFP<br>Lb8. 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>PCR:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT*<br>Lb13. Lc. lactis MG1363 pJP059*<br>*Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. <br><br><b>Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: </b><br>1.1. pSB1C3-red<br>18.2. pSB1C3-CHS_m<br>40. pEL3A17-red<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl<br>Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl<br>Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today.<br><br><h2>Other experiments</h2><br><b>Primer stock preparation</b><br><br><b>Dilution of sequencing primers: </b><br>3 tubes of 5 µM VF2-primer, 200 uL<br>3 tubes 5 µM VR-primer, 200 µL<br><br><b>Re-measuring concentration of earlier plasmid preps: *</b><br>1.4 CF48-pLR581: 127.8 ng/µl<br>1.5 CF48-pLR581: 215.9 ng/µl<br>3.2 DSM 20016-pVs2: 201.2 ng/µl<br>3.3 DSM 20016-pVs2: 139.1 ng/µl<br>3.10 DSM 20016-pVs2: 183.5 ng/µl<br>*We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind.<br><br><b>Preparing M17-broth, 950 ml.</b><br><b>(“) SOB</b><br><b>(“) CCMB80 (20% glycerol, pH 6,8)</b><br><b>Alliquoting dNTP, buffer, enzymes</b></div>'
+ }
+  else if(id == 'd2013623')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1><br><b>Name of participants: </b>Hampus E, Alexander W<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>4. 100-23-noplasmid<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>E. coli: </b><br>23.pCrcSB1C3-Crt-B<br>24.pSB1C3-Crt-I<br>25. pSB1C3-CrtY<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb5. plantarum 256 rifR-Pamβ1<br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><b>Restreak:</b><br>25. pSB1C3- crtY<br> <br><b>Plasmidpreparation </b><br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><h2>Result</h2><br><b>O/N:</b><br>25.pSB1C3-crtY: One of the overnights did not grow<br></div>'
+ }
+ else if(id == 'd2013622')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-06-22</h1><br><b>Name of participants: </b>Nafisa B, Mikael S, Marcus H<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb8. reuteri 100-23-pGT232<br>Lb9. faecalis  JH2-2- pAMβ1<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb14. reuteri DSM 20016-pLUL631 (B?)<br><br><b>O/N: </b><br>Lb4. reuteri100-23-noplasmid<br>Lb5. plantarum 256-rifR-pAMβ1<br><br><b>Plasmid preparation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb14. DSM 20016-pLUL631 (B?)<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br><b>L. reuteri:</b><br>Lb14.1 (strain 14, clone 1) DSM 20016-pLUL631 (B?): 10.0 ng/μl<br>Lb14.2 DSM 20016-pLUL631 (B?): 13.6 ng/μl<br>Lb14.3 DSM 20016-pLUL631 (B?): 14.7 ng/μl<br>Lb14.4 DSM 20016-pLUL631 (B?): 28,4 ng/μl<br>Lb6.1 DSM 20016-pLUL63TsA8: 18.7 ng/μl<br>Lb6.2 DSM 20016-pLUL63TsA8: 14.4 ng/μl<br>Lb6.3 DSM 20016-pLUL63TsA8: 12.5 ng/μl<br>Lb6.4 DSM 20016-pLUL63TsA8: 27.7 ng/μl<br>Lb7.2 DSM 20016-pGFP: 29.9 ng/μl<br>Lb7.3 DSM 20016-pGFP: 16.9 ng/μl<br>Lb7.4 DSM 20016-pGFP: 10.9 ng/μl</div>'
+ }
+   else if(id == 'd2013621')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-06-21</h1><br><b>Name of participants: </b>Stephanie H, Alona N, Anton B, Mikael S, Nafisa B, Anders E, Marcus H, Ken B-A, <br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work</h2><br><b>Plasmid preparation*: </b><br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><i>*to get more useable material and to look for different result from clones grown in a lock and a CO2-cupboard.</i><br><br><b>O/N: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb8. reuteri 100-23-pGT232<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb9. faecalis JH2-2 pAMβ1 *-<br><i>*from the plate without erythromycin. </i><br><br><b>Plasmid preparation:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>Re-streak: </b><br>Lb4. reuteri 100-23-noplasmid: No growth. → Grow on non-antibiotic plates  <br><br><b>Today:</b><br><b>Plasmid preparation:</b><br>Lb1.2 (strain 1, clone 2) reuteri CF48-pLR 581: Non-usable concentration *<br>Lb1.4 reuteri CF48-pLR 581: 161.8 ng/µl *<br>Lb3.8 reuteri DSM 20016 pVS2: 148.5 ng/µl *<br>Lb3.10 reuteri DSM 20016 pVS2: Non-usable concentration *<br><i>*there is seemingly no correlation between growth in lock vs. CO2-cupboard and plasmid concentration. This seems strange though. Cupboard is not supposed to create an anaerobic environment → do not use cupboard</i><br><br><h2>Other experiments</h2><br><b>SET buffer preparation: </b><br><b>Lysis buffer preparation: </b></div>'
+ }
+ else if(id == 'd2013620')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br><br><br><h2>Todays work:</h2><br><b>Glycerol stock preparation: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>Plasmid preparation:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>26. pEL3K15-red<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>O/N: </b><br>Lb2. reuteri 1063-pLUL631<br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><br><b>Streaking of new strains from Stefan Roos: </b><br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb12. reuteri DSM 20016-noplasmid *<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb4. reuteri 100-23-noplasmid<br>Lb8. reuteri 100-23-pGT232 *<br>Lb5. plantarum 256-rifR-pAMβ1<br>Lb10. plantarum 256-noplasmid *<br>Lb11. plantarum 36E-noplasmid *<br>Lb9. faecalis JH2-2 pAMβ1 **<br><i>*both on burned broth without glucose and CaCl2 and regular one.</i><br><i>**re-streaked on erythromycin and on antibiotic-free plates.</i><br><br><b>Transformation:</b><br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br><br><b>Restreaks:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br><br><h2>Results</h2><br><b>Previous day:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17)   <br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>19.1: 50.5 ng/µl  <br>19.2: 33.6 ng/µl<br>19.3: 45.2 ng/µl<br>19.4: 36.5 ng/µl<br>20.1: 28.9 ng/µl<br>20.2: 29.6 ng/µl<br>20.3: 41.8 ng/µl<br>20.4: 35.8 ng/µl<br>21.1: 47.7 ng/µl<br>21.2: 23.2 ng/µl<br>21.3: 38.8 ng/µl<br>21.4: 48.9 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again.<br>Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again.<br>Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl<br><br><br><h2>Other experiments</h2><br><b>Antibiotic-free MRS-broth plates preparation: </b><br><b>SET-buffer and Lysis-buffer preparation: </b><br><b>MRS-broth agar preparation: </b><br><b>Preparation of plates with and without antibiotic:</b> </div>'
+ }
+   else if(id == 'd2013619')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1><br><br><b>Name of participants: </b>Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br><br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red  <br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Spreading:</b> <br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>O/N:</b> <br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Transformation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day: </b><br><b>Spreading:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. <br><br><b>Transformation</b><br>1. pSB1C3-red: Successful<br>2. pSB3C5-red: Successful<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: Successful<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br><br><b>Restreak:</b><br>16. pSB1C3-TAL_m: Successful<br>17. pSB1C3-4Cl_m: Successful<br>18. pSB1C3-CHS_m: Successful<br>22. pSB1C3-B0032-BFP: Successful<br><br><b>Today:</b><br><b>O/N: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><b>Transformation:</b><br>23. pSB1C3-CrtB: fail<br>24. pSB1C3-CrtI: fail<br>25. pSB1C3-CrtY: fail<br><br><h2>Other experiments</h2><br><b>LB-agar plates </b><br><b>CaCl2-solution</b></div>'
+ }
+ else if(id == 'd2013618')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1><br><b>Name of participants: </b>Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br> <br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059   <br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work:</h2><br><b>Streaking of strains from Stefan Roos: </b><br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631 <br>Lb3. reuteri DSM 20016-pVS2<br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><b>Restreak</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Previous day</b><br><b>Restreak:</b><br>14. pET13b-zifz:4cl-PBSII:STS: Growth<br><br><b>Today</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail<br>16. pSB1C3-TAL_m: successful<br>17. pSB1C3-4Cl_m: successful<br>18. pSB1C3-CHS_m: successful<br>22. pSB1C3-B0032-BFP: successful<br><br><b>Restreak:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><h2>Other experiments:</h2><br><b>LB-agar</b></div>'
+ }
+else if(id == 'd2013617')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1><br><b>Name of participants: </b>Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Today:</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>14. pET13b-zifz:4cl-PBSII:STS: successful<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m: fail<br>17. pSB1C3-4Cl_m: fail<br>18. pSB1C3-CHS_m: fail<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful<br><br><h2>Other experiments:</h2><br><b>Mixing MRS-broth</b><br><b>Making of EDTA solution</b><br><b>MRS-plates preparation</b></div>'
+ }
+ else if(id == 'd2013613')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-13</h1><br><b>Name of participants:</b> Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F.<br><br><h2>Other experiments</h2><br><b>Making competent D5α</b></div>'
+ }
+   else if(id == 'd2013612')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-06-12</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F., Pontus D<br><br><h2>Other experiments</h2><br><b>LB-agar plates</b></div>'
+ }
+   else if(id == 'd2013610')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-10</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Nils A, Christoffer F., Pontus D, Niclas S.<br><br><h2>Other experiments:</h2><br><b>LB</b><br><b>CaCl2</b><br><b>Antibiotics</b></div>'
+ }
+ else if(id == 'd201373')
+ {
+  ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1><br><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A,  Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>1. pSB1C3-red<br>7. pSB3K3-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br><b>L. plantarum:</b><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br><br><br><b>L. reuteri:</b><br>Lb12. DSM 20016-noplasmid<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br><br><h2>Todays work</h2><br><b>PCR:</b><br>Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	  (colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	  (colony)*<br><i>*Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br>Lb13.2.2 lactis MG1363 pJP059	(plasmid)**<br>Lb13.2.3 lactis MG1363 pJP059	(plasmid)**<br>Lb13.3 lactis MG1363 pJP059		(colony)**<br>Lb13.4 Lc. lactis MG1363 pJP059	(colony)**<br><i>**Gel will be done tomorrow. Followed the PCR protocol with DMSO.</i><br>78. pEL3C18-CrtY*<br><i>-*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time)</i><br><br>Digest:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Ligation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>1. pSB1C3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>7. pSB3K3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><i>-*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning.</i><br><br><b>Transformation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77 pSB4C15-red<br><br><b>O/N:</b><br>Lb10.1. plantarum 256 no plasmid<br>Lb11.1. plantarum 36E no plasmid <br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br><br><b>Plasmid preparation:</b><br>7.pSB3K3<br>22.pSB1C3-B0032-BFP<br>42. pSB4S15-red<br><br><b>Gelelectrophoresis:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br>Lb13.2.3 Lc. lactis MG1363-pJP059*<br>Lb13.3 Lc. lactis MG1363-pJP059*<br>Lb13.4 Lc. lactis MG1363-pJP059*<br><i>*Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br><br><b>Results</b><br><b>Gelelectrophoresis:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><i>-Digestion Worked</i><br><br><b>failed:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	(colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	(colony)*<br><i>-*The gel showed nothing but primer dimer.</i><br><br><b>Digest:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Yesterdays transformation:</b><br><b>successful:</b><br>61. pSB1C3-CP1: Growth<br>61. pSB1C3-CP1 10x: Some growth<br>62. pSB1C3-CP8: Some growth<br>62. pSB1C3-CP8 10x: No growth<br>63. pSB1C3-CP11: Some growth<br>63. pSB1C3-CP11 10x: No growth<br>64. pSB1C3-CP29: Some growth<br>64. pSB1C3-CP29 10x: No growth<br>65. pSB1C3-CP30: Some growth<br>65. pSB1C3-CP30 10x: No growth<br>66. pSB1C3-CP41: Growth<br>66. pSB1C3-CP41 10x: Some growth<br>67. pSB1C3-Cp44: No growth<br>67. pSB1C3-Cp44 10x: No growth<br>68. pSB3K3-CP1-B0032-BFP: No growth<br>68. pSB3K3-CP1-B0032-BFP 10x: Growth<br>69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated<br>69. pSB3K3-CP8-B0032-BFP 10x: No growth<br>70. pSB3K3-CP11-B0032-BFP: Growth<br>70. pSB3K3-CP11-B0032-BFP 10x:  Growth<br>71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies<br>71. pSB3K3-CP29-B0032-BFP 10x: -----<br>72. pSB3K3-CP30-B0032-BFP: Growth<br>72. pSB3K3-CP30-B0032-BFP 10x: No growth<br>73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies<br>73. pSB3K3-CP41-B0032-BFP 10x: No growth<br>77.1 pSB4C15, clone 1: No growth<br>77.1 pSB4C15, clone 1 10x: No growth<br>77.2 pSB4C15, clone 2: Some  growth<br>77.2 pSB4C15, clone 2 10x: No growth<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>27. pEL3K16-red<br>38. pEL3A15-red<br>51. pEL3C18-red<br><br><b>failed:</b><br>81. pSB4S15-CP41: No growth<br>82. pSB4S15-CP29: No growth<br>83. pSB4S15-CP30: No growth<br><i>→ Redo ligation and transformation.</i><br><br><b>Plasmid preparation:</b><br>7.3  pSB3K3 clone 3: 50.1 ng/µl<br>7. 4 pSB3K3 clone 4: 43.4 ng/µl<br>7. 5 pSB3K3 clone 5: 48.1 ng/µl<br>22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl<br>22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ<br>22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ<br>42.1 pSB4S15-red clone 1: 43.6 ng/µl<br>42.2 pSB4S15-red clone 2: 46.1 ng/µl<br><br><h2>Other experiments</h2><br>Made agarplates with chloramphenicol resistance<br>MRS-broth preparation<br>MRS-broth-agar preparation <br>MRS-broth-agar plates preparation </div>';
+ }
+ else if(id == 'd201372')
+ {
+  ds = '<div id="dairy-text"><h1>Tuesday 2013-07-02</h1><br><b>Name of participants:</b> Christoffer Ahlström (CA), Nafisa Bashir (NB),  Viktor Törnblom (VT), Anton Berglund (AB), Mikael Strandgren (MS), Viktor Blomkvist (VB),  Thorsteinn O, Magnus B, Nils A, Alexander W, Christoffer F, Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli (strain D5α):</b>  <br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br>81. pSB4S15-CP41<br>82. pSB4S15-CP29<br>83. pSB4S15-CP30<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>L. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h2><br><b>O/N:</b><br>Lb9.7. faecalis JH2-2 pAMβ1 clone 7 (erythromycin) <br>Lb9.8. faecalis JH2-2 pAMβ1 clone 8 (no-antibiotic)<br>Lb9.9. faecalis JH2-2 pAMβ1 clone 9 (erythromycin)<br>Lb9.10. faecalis JH2-2 pAMβ1 clone 10 (no-antibiotic) <br>Lb10.1. plantarum 256 no plasmid<br>Lb11.1. plantarum 36E no plasmid<br>Lb12.1 reuteri DSM 20016<br>Lb19.5 L. thermophilus<br>Lb19.6 L. thermophilus<br>7.3. pSB3K3-red<br>7.4. pSB3K3-red<br>7.5. pSB3K3-red<br>22.1.1. pSB1C3<br>22.2.1. pSB1C3<br>22.2.2. pSB1C3<br>42.1.1. pSB4S15-red<br>42.1.2. pSB4S15-red<br>42.1.3. pSB4S15-red<br><br><b>Digest: </b><br>Lb9.4 faecalis JH2-2 pAMβ1<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>Gel electrophoresis: </b><br>Lb9.4 faecalis JH2-2 pAMβ1<br><br><b>Frozen stock: </b><br>Lb13.3. Lc. lactis MG1363 pJP059 clone 3<br>Lb13.4. Lc. lactis MG1363 pJP059 clone 4<br>Lb15.3. Lc. lactis MG1363 pGus clone 3<br>Lb15.4. Lc. lactis MG1363 pGus clone 4<br>Lb16.3. Lc. lactis MG1363 noplasmid clone 3<br>Lb16.4. Lc. lactis MG1363 noplasmid clone 4<br><br><b>Plasmid preparation: </b><br>Lb13.3. Lc. lactis MG1363 pJP059 clone 3<br>Lb13.4. Lc. lactis MG1363 pJP059 clone 4<br>Lb15.3. Lc. lactis MG1363 pGus clone 3<br>Lb15.4. Lc. lactis MG1363 pGus clone 4<br><br><b>PCR: </b><br>Lb13.1 Lc. lactis MG1363 pJP059<br>Lb13.2 Lc. lactis MG1363 pJP059<br>Lb13.3 Lc. lactis MG1363 pJP059<br>Lb13.4 Lc. lactis MG1363 pJP059<br>The primers for usp45 were used in order to amplify the usp45 signal peptide from the L. lactis chromosome. A gel was run to confirm fragment size.<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br><br><b>Gel electrophoresis: </b><br>Lb13.1 Lc. lactis MG1363 pJP059<br>Lb13.2 Lc. lactis MG1363 pJP059<br>Lb13.3 Lc. lactis MG1363 pJP059<br>Lb13.4 Lc. lactis MG1363 pJP059<br>1. pSB1C3-red		 		(cut with EcoRI and SpeI today)<br>1. pSB1C3-red 				(cut with EcoRI and SpeI saturday)<br>7. pSB3K3-red 				(cut with EcoRI and PstI today)<br>22. pSB1C3-B0032-BFP 			(cut with XbaI and PstI saturday)<br>42. pSB4S15-red 				(EcoRI and SpeI today)<br>42. pSB4S15-red 				(cut with SalI and SacI saturday)<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br><br><b>Re-streak from frozen stock: </b><br>Lb12.1 reuteri DSM 20016 no plasmid<br>Lb12.2 reuteri DSM 20016 no plasmid<br>Two re-streaks of each clone were made on two antibiotics free plates.<br><br><b>Re-streak: </b><br>Lb19.1 L. thermophilus<br>Lb19.2 L. thermophilus<br>Lb19.3 L. thermophilus<br>Lb19.4 L. thermophilus<br>Lb19.7 L. thermophilus<br>Lb19.8 L. thermophilus<br>Lb19.9 L. thermophilus<br>Lb19.10 L. thermophilus<br><br><b>Streaking of yogurt culture: </b><br>Lb18. Lb. delbrueckii bulgaricus  <br>Lb19. Lactococcus Thermophilus<br><br><b>Assembly and subcloning: </b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77.1 pSB4C15, clone 1<br>77.2 pSB4C15, clone 2 had less of plasmid 42 because it ran out.<br>81. pSB4S15-CP41<br>82. pSB4S15-CP29<br>83. pSB4S15-CP30<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>Transformation: </b><br>61. pSB1C3-CP1 *<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11 *<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP *<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP *<br>72. pSB3K3-CP30-B0032-BFP *<br>73. pSB3K3-CP41-B0032-BFP *<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15, clone 1<br>77.2 pSB4C15, clone 2 *<br>81. pSB4S15-CP41 *<br>82. pSB4S15-CP29<br>83. pSB4S15-CP30<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><i>* Those marked were incubated for maybe 25 minutes longer.</i><br><br><b>Subcloning</b> of 57 and 58 into pSB1C3 (from 1. pSB1C3-Red).<br>PCR of Crt Z(with 7% DMSO), 19, 20, 21(now with VF2, VR and culture)<br><b>Gelelectroforesis</b> of CrtE, CrtZ, 19, 20, 21<br><b>Transformation</b> of 23, 24, 27, 38, 51.<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N: </b><br>Lb13.3 Lc. lactis MG1363 pJP059 clone 3: Good growth, NC ok!<br>Lb13.4 Lc. lactis MG1363 pJP059 clone 4: Good growth, NC ok!<br>Lb15.3 Lc. lactis MG1363 pGus clone 3:   Good growth, NC ok!<br>Lb15.4 Lc. lactis MG1363 pGus clone 4:   Good growth, NC ok!<br><i>→ Continue with frozen stock and plasmid preparation.</i><br><br><b>Assembly and transformation: </b><br>61. pSB1C3-CP1:           Nothing<br>61. pSB1C3-CP1 10x:       Nothing<br>62. pSB1C3-CP8:           Growth<br>62. pSB1C3-CP8 10x:       Nothing<br>63. pSB1C3-CP11:          Growth<br>63. pSB1C3-CP11 10x:      Nothing<br>64. pSB1C3-CP29:          Growth<br>64. pSB1C3-CP29 10x:      Nothing<br>65. pSB1C3-CP30:          Growth<br>65. pSB1C3-CP30 10x:      Nothing<br>66. pSB1C3-CP41:          Nothing<br>66. pSB1C3-CP41 10x:      Nothing<br>67. pSB1C3-Cp44:          Growth<br>67. pSB1C3-Cp44 10x:      Nothing<br>77. pSB4C15-red:          Nothing <br>77. pSB4C15-red 10x:      Nothing<br>45. pSB1C3-B0034-4CL      Growth<br>46. pSB1C3-B0034-His-4CL  Growth<br>47. pSB1C3-B0034-STS      Growth<br>48. pSB1C3-B0034-His-STS  Growth<br><i>→ Redo with extra care, look over the protocols, use new ligase and ligation buffer.</i><br><br><b>Today:</b><br><b>Gel electrophoresis: </b><br>43. pSB1C3-B0034-TAL                                    Good bands<br>44. pSB1C3-B0034-His-TAL                                Good bands<br>Lb13.1 Lc. lactis MG1363-pJP059				<br>13.2 Lc. lactis MG1363-pJP059					<br>13.3 Lc. lactis MG1363-pJP059<br>13.4 Lc. lactis MG1363-pJP059<br>Lb9.4 faecalis JH2-2 pAMβ1                              Faint blurry bands*<br>1. pSB1C3-red (cut with EcoRI and SpeI today)           Good bands<br>1. pSB1C3-red (cut with EcoRI and SpeI saturday)        Good bands<br>7. pSB3K3-red (cut with EcoRI and PstI today)           Good bands<br>22. pSB1C3-B0032-BFP (cut with XbaI and PstI saturday)  Nothing**<br>42. pSB4S15-red (EcoRI and SpeI today)                  Nothing**<br>42. pSB4S15-red (cut with SalI and SacI saturday)       Nothing**<br><i>→ 1 and 7 were ok, they could be reused.</i><br><br><i>*The results were consistent with yesterdays Gel electrophoresis: Very faint smudged bands that lets us think there is only chromosomal DNA in this plasmid preparation.<br>**The results indicate that something may be wrong with the digests of 42. and 22., this may be one of the explanations of why we got no colonies of 77. but many of 61-67.</i><br><br><b>Plasmid preparation: </b><br>Lb13.3. Lc. lactis MG1363 pJP059 clone 3: 13.4 ng/µl<br>Lb13.4. Lc. lactis MG1363 pJP059 clone 4: 10.3 ng/µl<br>Lb15.3. Lc. lactis MG1363 pGus clone 3: 11.9 ng/µl<br>Lb15.4. Lc. lactis MG1363 pGus clone 4: 12.0 ng/µl<br><i>→ Dispose, to low concentration. Re-do a plasmid preparation from O/N with optimized conditions. </i><br><br>Gel showed fail of PCR for 19,20, 21<br>Gel showed succes of CrtE, but fail of CrtZ(no primer dimers tho)<br>Plates from yesterday showed fail of subclone/transformation<br><br><h2>Other experiments</h2><br>Made agarplates with chloramphenicol resistance<br><b>Microscopy:</b><br>Confirming Lc.thermophilus colonies on the yoghurt-plate from the previous day.<br>Confirming contamination on some plates with  competent cells.<br><br>Primer design of lambda red, Crt Y, I and B both F and R<br></div>';
+ }
+ else if(id == 'd201371')
+ {
+  ds = '<div id="dairy-text"><h1>Monday 2013-07-01</h1><br><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Christoffer, Nils, Magnus, Alexander,  Anders Edlund (AE), Mikael Strandgren (MS), Christoffer Ahlström (CA), Viktor Blomkvist (VB), Viktor Törnblom(VT), Nafisa Bashir (NB), Anton Berglund (AB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>7. pSB3K3-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br>1. pSB1C3-red<br>60.PK401 dam-<br><br><b>L. plantarum:</b><br>Lb5.  256-rifR-pAMβ1<br><br><b>E. faecalis:</b><br>Lb9. JH2-2 pAMβ1<br><br><b>L. reuteri:</b><br>Lb12. DSM 20016-noplasmid<br><br><b>L. lactis:</b><br>Lb15 unknown-pGus<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>L. thermophilus:</b><br>19. L.thermophilus<br><br><h2>Todays work</h2><br><b>Gel electrophoresis:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>Lb9.4 faecalis JH2-2 pAMβ1<br>CBP amplicon from reuteri DSM 20016<br>usp45 amplicon from lactis strain MG1363<br><br><b>PCR:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS <br>6 colonies of DSM 20016 strain reuteri<br>4 colonies of MG1363 strain lactis<br>The PCR was carried out with the CBP primers for reuteri and the usp45 primers for lactis. This in order to isolate the signal peptides from each species.<br><br><b>O/N: </b><br>Lb13. Lc. lactis MG1363 pJP059 clone 3<br>Lb13. Lc. lactis MG1363 pJP059 clone 4<br>Lb15. Lc. lactis MG1363 pGus clone 3<br>Lb15. Lc. lactis MG1363 pGus clone 4<br>7. pSB3K3-red<br><br><b>Frozen stock:</b><br>Lb5.2 plantarum 256 pAMβ1<br>Lb9. faecalis JH2-2 pAMβ1 clone 4<br>1. pSB1C3-red<br>60.PK401 dam<br><br><b>Re-streak: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>Lb12. reuteri DSM20016 no plasmid<br><br><b>Plasmid preparation:</b><br>Lb5.2 plantarum 256 pAMβ1<br>Lb9. faecalis JH2-2 pAMβ1 clone 4<br>1. pSB1C3-red<br><br><b>Digest: </b><br>Lb9.4 faecalis JH2-2 pAMβ1 (To rule out chromosomal contamination in prep)<br><br><b>Assembly: </b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br><b>Transformation: </b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red*<br>*We made a transformation with one sample of 77-digest from today, and one from yesterday to try to see if the problem yesterday laid with the assembly or with the transformation<br><br><b>Streaking of yogurt culture: </b><br>18. Lb. delbrueckii bulgaricus  <br>19. Lactococcus Thermophilus<br><br><b>PCR</b> of Crt E, Crt Z, 19, 20, 21, Idi<br><b>Gelelectroforisis</b> of Crt E, Crt Z, Idi <br><b>Transformation</b> of 75, 76, 78, 58<br><b>Subcloning</b> of IspA ->58<br><b>Digest</b> of Idi<br><b>Ligation</b> of 76, 78<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>Assembly and transformation: </b><br>61. pSB1C3-CP1:  No growth<br>62. pSB1C3-CP8:  No growth<br>63. pSB1C3-CP11: No growth<br>64. pSB1C3-CP29: No growth<br>65. pSB1C3-CP30: No growth <br>66. pSB1C3-CP41: No growth<br>67. pSB1C3-Cp44: No growth<br>77. pSB4C15-red: No growth<br><i>→ Re-do all steps, but do one transformation with ligations from yesterday</i><br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>Lb5.2 plantarum 256 pAMβ1 clone 2: 50.7 ng/µl<br>Lb9.4 faecalis JH2-2 pAMβ1 clone 4: 403.7 ng/µl<br>1.1 pSB1C3-red clone 1: 144.0 ng/µl<br>1.2 pSB1C3-red clone 2: 96.8 ng/µl<br><br><b>Gel electrophoresis: </b><br>Lb9.4 faecalis JH2-2 pAMβ1*<br><i>*Very faint smudges that could be a shadow from the dye. Will follow up tomorrow with another gel electrophoresis of a new digest.</i><br>CBP amplicon from reuteri DSM 20016**<br>usp45 amplicon from lactis strain MG1363**<br><i>**None of the PCRs showed the desired bands on the gel.</i><br><br><b>PCR with good results:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL (Note: weak band)<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS  <br><i>- Very good results on the pcr</i><br><br>Gel of Crt E and Crt Z was failed at Ethylium Bromide bath. Redo gel<br>Gel of Idi showed succes, proceed to subclone. <br>Subclone of IspA showed only red colonies. IspA was incorrectly digested with E instead of X.<br><i>-dunno what this is! :S</i><br><br><h2>Other experiments</h2><br>M17-broth agar preparation<br>M17-broth medium preparation<br>SET-buffer preparation<br>Preparation of M17 plates</div>';
+ }
+ else if(id == 'd2013721')
+ {
+ds = '<div id="dairy-text"><h1>Sunday 2013-07-21</h1><p><strong>Name of participants:</strong>Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Viktor @, Marcus H. Nils, Christoffer, Anders Edlund (AE)<br><br>'+
+'<h2>Ongoing constructs:</h2>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>'+
+'99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br>'+
+'<strong>L. reuteri:</strong><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><strong>L. plantarum:</strong><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br>'+
+'<h2>Todays work:</h2><b>Plasmid Prep on:</b><br>117.2  pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br>120.1 pSB1C3-B0034-HIS-4CL<br>104.2 and 104.5 pSB1C3-B0034-4CL-B0034-STS<br>106.5 and 106.6 pSB3T5-J23110-B0034-TAL<br>122.2 pSB1C3-B0034-His-STS;<br><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>Gel:</b><br>21. CrtE~I/w RBS,ZF<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>128.pSIM5-tet (E.Coli Nissle) Endast O/N<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br>'+
+'<b>Overnight:</b><br>84.1 pSB4S15-CP6<br>84.2 pSB4S15-CP6<br>86.1 pSB4S15-CP6-B0032-BFP<br>86.2 pSB4S15-CP6-B0032-BFP<br>87(1.1) pSB4S15-CP25-B0032-BFP<br>87(1.2) pSB4S15-CP25-B0032-BFP<br>88.1 pSB4S15-CP1-B0032-BFP<br>88.2 pSB4S15-CP1-B0032-BFP<br>89.1 pSB4S15-CP44-B0032-BFP<br>89.2 pSB4S15-CP44-B0032-BFP<br>97.1 pSB4S15-CP8-B0032-BFP<br>97.2 pSB4S15-CP8-B0032-BFP<br>98.1 pSB4S15-CP11-B0032-BFP<br>98.2 pSB4S15-CP11-B0032-BFP<br><br>'+
+'<h2>Results:</h2><br><b>Re-streak:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger - Unable, no clones<br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger - 3 clones restreaked<br><br><b>Gel</b><br>21. CrtE~I/w RBS,ZF - fail<br><br><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA - Fail of all clones<br>109. pSB4A15-K206000-B0034-IspA - Fail of all clones<br>110. pSB4A15-K206001-B0034-IspA - Fail of all clones<br><br></div>';
+ }
+ else if(id == 'd2013720')
+ {
+ds = '<div id="dairy-text"><h1>Saturday 2013-07-20</h1><b>Name of participants:</b> Hampus.E, Kristoffer.L, Malin .B, Magnus, Nils, Pontus, Anders Edlund (AE), Stephanie Herman (SH)<br><br><h2>Ongoing constructs:</h2>99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h2><b>Re-streak:</b><br>84. pSB4S15-CP6<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>99.  pSB1C3-CrtO<br><br><b>Overnigth:</b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>111.6 pSBLb4C15-red<br>111.7 pSBLb4C15-red<br><br><b>Transformation: </b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger <br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger <br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>134. pSB1C3-CBP<br>135. pSB1C3-CSP<br>136. pSB1C3-USP45<br><br><b>Plasmidprep:</b><br>109. pSB4A15-K206000-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>1.1? 90.3 j23101<br>1.2?<br>2.1?90.4 j23101<br>2.2?<br><br><b>Gel:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening pcr of constructs:</b><br>119.pSB1C3-B0034-4CL<br>120 pSB1C3-B0034-HIS-4CL<br>106 pSB3T5-J23110-B0034-TAL<br>117 pSB3T5-CP1-B0034-His-TAL<br>107 pSB3T5-CP1-B0034-TAL<br><br><b>PCR:</b><br>21. pSB4C5-pBAD/AraC-CrtE~I/w RBS, ZF (to remove promotor)<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><h2>Results</h2><b>Overnight & screening:</b><br>99.  pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><br><b>Screening of: </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br><i>showed negative results on all clones but 109.2. A new screening PCR of previously un-screened clones was prepared to run overnight.</i><b>Gel<br><br>Gel succes on: </b><br>106.5 pSB3T5-J23110-B0034-TAL<br>106.6 pSB3T5-J23110-B0034-TAL<br>117.1 pSB3T5-CP1-B0034-His-TAL<br>117.2 pSB3T5-CP1-B0034-His-TAL<br>119.2 pSB1C3-B0034-4CL<br><b>No band on:</b><br>107 pSB3T5-CP1-B0034-TAL<br>116 pSB3T5-J23110-B0034-His-TAL<br>120 pSB1C3-B0034-HIS-4CL<br></div>';
+ }
+  else if(id == 'd2013719')
+ {
+ds = '<div id="dairy-text"><h1>Friday 2013-07-19</h1><br>Name of participants: Ken B-A, Kristoffer L, Malin, Magnus, Sabri, Nils, Pontus, <br>Christoffer Ahlström (CA), Stephanie Herman (SH) <br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α): </b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br> 107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>136.1 pSB1C3-usp45<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><h2>Todays work</h2><b>Screening pcr of constructs:</b><br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>117. pSB3T5-CP1-B0034-His-Tal<br>119. pSB1C3-B0034-4CL (from 15)<br>120. pSB1C3-B0034-His-4CL (from 15)<br><br><b>Overnight & screening:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>Plasmid preparation:</b><br>122.2. pSB1C3-B0034-His-STS (from 15)<br>90.3. BBa_J61002 (amp)-J23101<br>90.4. BBa_J61002 (amp)-J23101<br>104.2. pSB1C3-B0034-4CL-B0034-STS <br>104.5. pSB1C3-B0034-4CL-B0034-STS<br>111.2. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.3. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>111.4. pSBLb4C15-E.coli ori replaced with ori from lactobacillus (pJP059) in pEL4C15<br>121.1. pSB1C3-B0034-STS (from 15)<br>121.2. pSB1C3-B0034-STS (from 15)<br><br><b>Cloning:</b><br>21. CrtE~I /w RBS, Zinc finger → 1. pSB1C3-red<br><br><b>Assembly:</b><br>21. CrtE~I /w RBS, Zinc finger, 91. BBa-J61002-J23106 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 92. BBa-J61002-J23110 → 11. pSB4A15-red <br>21. CrtE~I /w RBS, Zinc finger, 103. BBa-J61002-J23101 → 11. pSB4A15-red <br><br><<b>Gel electrophoresis:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Restreak</b><br>128. pSIM5-tet(Nissle)<br><br><h2>Results:</h2><b>Screening: </b><br> 108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><i>showed negative results on all clones but 109.2, which proceeded to overnight. A new screening PCR was prepared to run overnight.</i><br><br><i>showed positive results in all cases but 125.4 and 127.3. All other clones proceeded to overnight.</i><br><br><i>The overnight showed no contamination of negative controls, and growth in all clones but 127.1 and 127.2.</i><br><br><b>Transformation:</b><br>Only visual red colonies on CBP & CSP and possible 2 white colonies on usp45.<br>→ Re-do the transformation with the saved ligation solution from (18/07-13)<br>→ Re-streak the possible white colonies. <br><br></div>';
+ }
+  else if(id == 'd2013718')
+ {
+ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Alexander, Magnus, Pontus,Sabri,StephanieHerman (SH), Alona Nyberg (AN), Christoffer Ahlström (CA), Viktor Blomkvist (VB)<br><br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>90. BBa_J61002-J23101<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br>Ligation<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Gel-electrophoresis of screening PCR products</b><br>42. pSB4S15-red<br><br><b>Digestion:</b><br>42. pSB4S15-red<br><br><b>Transformation:</b><br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Overnight:</b> <br>90. BBa_J61002-J23101<br><br><b>Spreading of strains:</b> <br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Re-circulization:</b><br>129. pSB1C3-Idi (mut)<br><br><b>Plasmid Prep (Glycerol stock):</b><br>75. pEL3A15 - CrtB (75.1, 75.2, 75.4)<br><br><b>Transformation:</b><br>99. pSB1C3 - Crto <br>129. pSB1C3-Idi (mut)<br>113. E.coli Nissle-pSIM5-tet<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>Re-streak:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><br><b>Ligation of strains from yesterdays digest:</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br>*Ligated to 1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b>*<br>Collagen binding protein<br>Cell surface protein <br>usp45 <br><br>*Performed with D5(alpha) competent cells. <br><br><b>Screening:</b><br>111. pSBLb4C15<br><br><b>Spreading of strains:</b><br>Collagen binding protein [D5(alpha)] *<br>Cell surface protein [D5(alpha)] *<br>usp45 [D5(alpha)] *<br>111. pSBLb4C15 **<br>112. pSBLbEc4C15 **<br><br>*With a 10x and 1x dilution. <br><br>** 2 plates of each with 1x dilution.<br><br><h2>Results</h2><b>Re-streaks:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>were successful on all clones but 108.7.<br><br>Transformation of 129. pSB1C3-Idi (mut) did not grow.<br><br><b>Electrophoresis on digests:</b> <br>*The gel showed that the components (signal peptides and destination plasmid) had been cut and therefore have the digest succeeded. <br><br></div>';
+ }
+   else if(id == 'd2013717')
+ {
+ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-18</h1><br><br><b>Name of participants:</b> Karl H, Ken B-A, Kristoffer L, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>57.Psb1C3-B0034-Idi<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. Lc.thermophilus<br><br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><br><b>Ligation</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-His-4CL<br><br><b>Digest & Ligation:</b><br>ZCD + 1. pSB1C3-Red -> 123. pSB1C3-ZCD<br>ZCD-His + 1. pSB1C3-Red -> 125. pSB1C3-ZCD-His<br>UGTCs2 + 1. pSB1C3-Red -> 126. pSB1C3-UGTCs2<br>B0034-UGTCs2-His + 4. pSB1A3-Red -> 127. pSB1A3-B0034-UGTCs2-His<br><br><b>Digest</b><br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br>1.1 pSB1C3-red (From strain database 2013)<br><br><b>Transformation:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br><br><b>O/N & Screening:</b><br>75. pEL3A15 - CrtB<br>102. pSB1C3 - CrtE<br><br><b>Plasmid Prep(Glycerol stock)</b><br>79. pEL3K16 - CrtE <br>101. pSB1C3 - DNA program123456<br><br><b>PCR</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF (E,P)(To remove promotor)<br>57.pSB1C3-B0034-Idi mutagenesis (Phusion) to remove restriction site. <br><br><b>Gel-electrophoresis of screening PCR products:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Sequencing preparation:</b><br>62. pSB1C3-CP8<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP <br><br>Gel-electrophoresis of overnight PCR products:<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1<br><br><b>Re-streak: </b><br>Lb18. Lb. delbrueckii bulgaricus no plasmid<br><br><b>Spreading of strains: </b><br>Lb19. Lc.thermophilus no plasmid*<br><br>*With 1x, 10x & 50x dilution<br><br><br><h2>Results</h2>57.pSB1C3-B0034-Idi worked, gel run showed a band at suspected size , but also some smudge, probably primer dimers.<br>101. pSB1C3 - DNA program123456 worked and sent for sequence<br><br><b>Transformations:</b><br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>123. pSB1C3-ZCD<br>125. pSB1C3-ZCD-His<br>126. pSB1C3-UGTCs2<br>127. pSB1A3-B0034-UGTCs2-His<br>all grew red and white colonies, indicating successful ligation and transformation.<br><br><b>Gel-electrophoresis of overnight PCR products:</b><br>Collagen binding protein → cloned from strain 14.4: NC checks good and lines are equal to ~200bp.<br>Cell surface protein → cloned from strain 3.1: NC checks good and lines are equal to ~150bp.<br>usp45 → cloned from strain 13.1: NC checks good and lines are equal to ~140bp.<br><br></div>';
+ }
+   else if(id == 'd2013716')
+ {
+ds = '<div id="dairy-text"><br><h1>Tuesday 2013-07-16</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Kristoffer L, Christoffer .F, Sabri. J, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas, Viktor Blomkvist(VB), Alona Nyberg (AN), Christoffer Ahlström (CA)<br><br><h2>Ongoing constructs:</h2><br><br><b>E.coli (strain D5α):</b><br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>75. pEL3A15 - CrtB<br>99. pSB1C3 - CrtO<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br>96. Cs42s-Promoter-ZCD-PsbA2-UGTCs2-PsbA2-Beta-carotene hydroxylase-B1002<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi <br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><h2>Todays work</h2><b>Assembly</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br><br><b>Ligation:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel on Digest:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br><br><b>Restreak:</b><br>47. pSB1C3-B0034-STS<br>75. pEL3A15 - CrtB<br><br><b>Screening:</b> <br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP<br>27. pEL3K16-red<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><b>Transformation:</b><br>99. pSB1C3 - CrtO<br><br><b>O/N & Screening</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>101. pSB1C3 - DNA program123456<br><br><b>Gelelectrophoresis</b><br>21. pSB4C5 - pBAD/AraC-CrtE~I /w RBS, ZF<br>57. PSB1C3-B0034-Idi (on pcr product from overnight pcr with new protocol) <br><br><b>PCR </b><br>57.PSB1C3-B0034-IDI (overnight Tuesday-Wednesday new protocol) <br><br><h2>Results</h2><b>Plasmid preparation: </b><br>74. pSB3K3-CP44-B0032-BFP: Failed! → Re-do!<br><br><b>Screening:</b> <br>111. pSBLb4C15: Failed<br>112. pSBLbEc4C15: Failed<br><br><h2>Other experiments</h2>Primer stock preparation: CA *<br>CSP_RF<br>CBP_RF<br>usp45_RF<br>CBP F<br>*Obtained a concentration of 5µM at a volume of 200µl.<br><br></div>';
+ }
+ else if(id == 'd2013715')
+ {
+ds = '<div id="dairy-text"><br><h1>Monday 2013-07-15</h1><br><br><b>Name of participants:</b> Karl H, Lovisa P, Theodor L, Ken B-A, Victor S, Hampus.E, Christoffer, Sabri, Alexander, Nils, Thorsteinn, Magnus, Malin, Pontus, Niclas<br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>105. pSB3T5-J23101-B0034-TAL<br>104. pSB1C3-B0034-4CL-B0034-STS<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br>101. pSB1C3 - DNA program123456<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>99. pSB1C3 - CrtO<br>57.Psb1C3-B0034-Idi<br>91. BBa_J61002 (amp)-J23106<br>94. pSB1C3-K206000<br>95. pSB1C3-K206001<br>11. pSB4A15-blue(?)<br>58. pSB1C3-IspA<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br>22. Betacarotene-various plasmids<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-p256<br><br><h2>Todays work</h2><b>PCR Purification:</b><br>111. pSBLb4C15, digest<br>112. pSBLbEc4C15, digest<br><br><b>Ligation: </b><br>111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) <br>112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI)<br><br><b>Transformation: </b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>75. pEL3A15 - CrtB<br>99. pSB1c3 - CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Overnight: </b><br>74. pSB3K3-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><b>Fluorescence(BFP) experiment with UV: </b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Plasmid preparation: </b><br>Lb10.1 plantarum 256 p256<br>Lb10.2 plantarum 256 p256<br>Lb10.3 plantarum 256 p256<br>Lb22.1 reuteri betacarotene (strain name?) various plasmids<br>44.5 pSB1C3-b0034-His-Tal<br>74. pSB3K3-CP44-B0032-BFP<br><br><b>PCR:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Gel electhrophoresis</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Assembly:</b><br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br><br><b>Re-streak:</b><br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br><br><br><b>PCR cloning of:</b><br>CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 20. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtE~(O)-Z /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 19. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger<br><br><b>Mutagenesis</b> <br>57. pSB1C3-B0034-Idi <br><br><b>Sequence preperation</b><br>78.1 pEL3C18-CrtY (VF and VR)<br>78.3 pEL3C18-CrtY (VF and VR)<br><br><b>Screening:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>101. pSB1C3 - DNA program123456 <br><br><i>Touchdown PCR with Phusion polymerase (signal peptides): CA<br>Collagen binding protein → cloned from strain 14.4<br>Cell surface protein → cloned from strain 3.1<br>usp45 → cloned from strain 13.1</i><br><br><h2>Results</h2><b>PCR, Succes on:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>Transformation </b><br>45. pSB1C3-B0034-4CL, failed<br>46. pSB1C3-B0034-His-4CL, failed<br>104. pSB1C3-B0034-4CL-B0034-STS, failed<br>106. pSB3T5-J23110-B0034-TAL, failed<br>108. pSB4A15-J23106-B0034-IspA, failed<br>109. pSB4A15-K206000-B0034-IspA, failed<br>110. pSB4A15-K206001-B0034-IspA, failed<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><i>-(45,46,104,106) Probably due to weak competent cells<br>-(111-112) No growth. Possible problems: Too short time for proper ligation, no purification before ligation. Another attempt will be madwith the ligation from yesterday and a new one with purified digests.<br><br>Nanodrop measurement of 57.Psb1C3-B0034-Idi was 80 ng/microliter.</i><br><br><b>Gel:</b><br>21 pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268, fail<br><br><b>Fluorescence(BFP) experiment with UV:</b><br>68. pSB3K3-CP1-B0032-BFP : Glowing (68.2 68.4)<br>69. pSB3K3-CP8-B0032-BFP : Glowing (69.3 69.4)<br>70. pSB3K3-CP11-B0032-BFP : Glowing (70.3)<br>71. pSB3K3-CP29-B0032-BFP : Glowing (71.1 71.2)<br>72. pSB3K3-CP30-B0032-BFP : Glowing (72.1 72.2)<br>73. pSB3K3-CP41-B0032-BFP : Glowing (73.4)<br>74. pSB3K3-CP44-B0032-BFP : Glowing (74.1.1 74.1.2)<br>81. pSB4S15-CP41-B0032-BFP : Not glowing<br>83. pSB4S15-CP30-B0032-BFP : Not glowing<br>86. pSB4S15-CP6-B0032-BFP : Not glowing<br>88. pSB4S15-CP1-B0032-BFP : Not glowing<br>89. pSB4S15-CP44-B0032-BFP : Not glowing<br><br><b>Plasmid preparation:</b><br>Lb10.1 plantarum 256 p256: 					124.7 ng/µl<br>Lb10.2 plantarum 256 p256: 					23.5 ng/µl<br>Lb10.3 plantarum 256 p256: 					270.9 ng/µl <br>Lb22.1 reuteri betacarotene (strain name?) various plasmids: 51.4 ng/µl<br><br></div>';
+ }
+ else if(id == 'd2013714')
+ {
+  ds = '<div id="dairy-text"><h1>Sunday 2013-07-14</h1><b>Name of participants:</b> Anders Edlund (AE), Viktor Blomkvist (VB), Lovisa P, Christoffer F, Sabri J<br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):  </b><br>7.pSB3k3-red<br>22. B0032-BFP<br>42. pSB4S15-red<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110<br>93. BBa_J61002-J23116<br>103. pSB1k3-J23101<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br>101. pSB1C3 - DNA program123456<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br><br><b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>14. DSM 20016-pLUL631(B?)<br>22. Betacarotene-various plasmids<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-p256<br><br><h2>Todays work</h2><b>Ligation: </b><br>111. pSBLb4C15 (MluI+NheI) + Ori from pJP059 (MluI+NheI) <br>112. pSBLbEc4C15: (MluI+ClaI) + Ori from pJP059 (MluI+ClaI)<br><br><b>Transformation:</b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>75. pEL3A15 - CrtB<br><br><b>Plasmid prep. and glycerol stock:</b><br>103. pSB1k3-J23101<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110<br>93. BBa_J61002-J23116<br>44.4 pSB1C3-b0034-His-Tal<br>76.(1-4)-pEL3K16-CrtI<br>78.(1-3)-pEL3C18-CrtY<br><br><b>Overnight:</b><br>44.5 pSB1C3-b0034-His-Tal<br>BBa_J61002-J23101 (amp)<br><br><b>Restreak:</b><br>101. pSB1C3 - DNA program123456 (Picked 4 clones)<br><br><b>Subcloning:</b><br>CrtE (PCR prod) should be digested with X,P (Can be found in digest box)<br>27. pEL3K16 - red should be digested with X,P → CrtE (PCR prod)<br>1. pSB1C3 - red should be digested with X,P → CrtE (PCR prod)<br>38. pEL3A15 - red → 23. pSB1C3-CrtB<br><br><b>PCR cloning:</b><br>CrtE~I /w RBS, Zinc finger (PCR is done to remove promoter). Template should be the following construct, digested (E,P) 21. pBAD/AraC-CrtE~I /w RBS, Zinc finger <br><br>CrtZ (PCR is done to clone and biobrick CrtZ from the following template, 20. pBAD/AraC-CrtE~CrtZ /w RBS, Zinc Finger<br><br>CrtO PCR is done to clone CrtO from the following template, digested (E,P) 19. pBAD/AraC-CrtE~Crt(O)-Crt(Z) /w RBS, Zinc finger<br><br><h2>Results</h2>Problems with the yesterday ligation, the experiment was redone<br>Transformation of 101. DNA program123456 was a sucess although 102. pSB1C3-CrtE failed<br>The PCR failed for CrtE~I, unspecific binding<br>The PCR failed for CrtZ, unspecific binding. <br></div>';
+ }
+ else if(id == 'd2013713')
+ {
+  ds = '<div id="dairy-text"><br><h1>Saturday 2013-07-13</h1><b>Name of participants:</b> Sabri J, Nils, Kristoffer L, Theodor Löwe, Viktor Blomkvist (VB), Stephanie Herman (SH), Alona Nyberg (AN)<br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>101. pSB1C3 - DNA Program123456<br>79. pEL3K16 - CrtE <br>102. pSB1C3 - CrtE<br>76. pEL3K16 - CrtI <br>78. pEL3C18 - CrtY<br>90 pSB1k3-J23101<br>91 BBa_J61002-J23106<br>92 BBa_J61002-J23110<br>93 BBa_J61002-J23116<br>44.4 pSB1C3-b0034-His-Tal<br>44.5 pSB1C3-b0034-His-Tal<br>111. pSBLb4C15: MluI+NheI<br>112. pSBLbEc4C15: MluI+ClaI<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br><br><h2>Todays work</h2><b>Ligation:</b><br>1. pSB1C3 - red → DNA program123456 (PCR prod)<br>1. pSB1C3 - red → CrtE (PCR prod)<br>27. pEL3K16 - red → CrtE (PCR prod)<br><br><b>PCR and Gelelectrophoresis:</b><br>CrtO<br><br><b>Transformation:</b><br>101. pSB1C3 - DNA program123456<br>102. pSB1C3 - CrtE<br><br><b>Overnight, Screening:</b><br>76.(1-4) pEL3K16 - CrtI <br>78.(1-3) pEL3C18 - CrtY<br><br><b>PCR purification: </b><br>111. pSBLb4C15<br>112. pSBLbEc4C15<br><br><b>Digestion:</b><br>1. pSB1C3 - red → DNA program123456 (PCR prod)<br>111. pSBLb4C15: MluI+NheI<br>112. pSBLbEc4C15: MluI+ClaI<br>Lb13. Lc. lactis MG1363-pJP059, <i>Ori: Two experiments, Mlu1+NheI & MLuI+ClaI<br>* Parts of what will become the first shuttle-vector.</i><br><br><b>Plasmid preparation:</b><br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br><br><h2>Results</h2><b>Gel:</b><br>47. pSB1C3-B0034-STS PCR product with and without DMSO (7%)<br>Good results on GEL, both PCR with and without DMSO looks good.<br><br><img class="result-pic" src="https://static.igem.org/mediawiki/igem.org/4/43/Uppsala_gelPic_2013-07-13.jpg"><table class="table-pic-desc-table"><tr><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td><td>9</td><td>10</td></tr><tr><td>N/C</td><td>76.1 (Sucess)</td><td>76.2 (Sucess)</td><td>76.3 (Sucess)</td><td>76.4 (Sucess)</td><td>Ladder</td><td>N/C</td><td>78.1 (Sucess)</td><td>78.2 (Sucess)</td><td>78.3 (Sucess)</td></tr><tr><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td></tr><tr><td>Ladder</td><td>CrtE (digested with E,P)</td><td>CrtE~I (digested with X, P) (Failed)</td><td>CrtE~I (digested with E, P) (Failed)</td><td>N/C</td><td>CrtO (Failed)</td><td>ORI I (Geneticals)</td><td>ORI II (Geneticals)</td><td>Ladder</td></tr></table><br><i>CrtE PCR prod should have been digested with X,P therefore construct 79 and 102 needs to be redone </i><br><br></div>';
+ }
+else if(id == 'd2013712')
+ {
+  ds = '<div id="dairy-text"><br><h1>Friday 2013-07-12</h1><b>Name of participants:</b> Kristoffer L, Emil M, Karl H, Peter C, Lovisa P, Theodor L, Ken B-A, Christoffer F, Sabri J, Niclas<br><br><h2>Ongoing constructs:</h2><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>82. pSB4S15-CP29-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>87. pSB4S15-CP25-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br><b>Lc. thermophilus:</b><br>19. L.thermophilus<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-p256<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><h2>Todays work</h2>Digestion:<br>22. B0032-BFP - E,P<br>11. psB4A15<br><br><b>Ligation:</b> <br>CrtE~I /w RBS + Zincfinger -> 11. pSB4A15-ed<br>CrtE(PCR-prod.) -> 27. pEL3K16-red <br>CrtE -> 1. pSB1C3 (to registry)<br><br><i>-all AMP plates from yesterday failed 90-93. (J23 series promotors)<br>Digest of construct 14 and 15 from plasmid prep with Pst1, followed by gel analysis.</i><br><br><b>Transformation:</b><br>CrtE~I /w RBS + Zincfinger -> 11. pSB4A15-ed<br>CrtE(PCR-prod.) -> 27. pEL3K16-red <br>82. pSB4S15-CP29-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>87. pSB4S15-CP25-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>100. pJP059<br>J23101<br><br>PCR on DNA program 123456<br><br>Plasmid prepp on 96.Cs42s clone nr 1 and 2. <br>Glycerol stock on 96.Cs42s clone 1 and 2.<br>Gel run on digest from plasmid preparation of 96.cs42s clone 1 and 2.<br><br><b>Re-streak:</b><br>76 pEL3K16 - CrtI (4 clones)<br>78. pEL3C18 - CrtY (3 clones)<br>74. pSB3K3-CP44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br><br><b>Gel electrophoresis:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>46. pSB1C3-B0034-His-4CL<br>48. pSB1C3-B0034-His-STS<br>pSB4C15 PCR-product with intact ori<br>pSB4C15 PCR-product without ori<br><br><b>Overnight culture:</b><br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>Lb10.3 plantarum 256 p256<br>Lb10.4 plantarum 256 p256<br><br><b>Spreading:</b><br>87. pSB4S15-CP25-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>Lb10.3 plantarum 256 p256<br>Lb10.4 plantarum 256 p256<br><br><h2>Results</h2><b>Transformation:</b><br>Successful:<br>76 pEL3K16-CrtI<br>78 pEL3C18-CrtY<br> <br>failed:<br>75  pEL3A15-CrtB<br><br>The result from nanodrop measurement of 96.Cs42s clone 1 was 408.3 nanogram/microliter and clone 2 was 332. Also both had a god a260/a280 ratio.<br>The result from the gel run of the two 96.Cs42s clones indicates an construct with a size of ~4000 bp. <br><br><b>Gel on PCRs:</b><br>Successful:<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br><br>failed<br>47. pSB1C3-B0034-STS<br>46. pSB1C3-B0034-His-4CL<br>48. pSB1C3-B0034-His-STS<br><br><i>-PCR-amplification: 47. pSB1C3-B0034-STS, change temperature -2 degrees<br><br>Construct 14 had two bands on the gel, indicating illegal restriction sites. Construct 15 only had one band and no indication of illegal restriction sites.</i><br><br><h2>Other experiments</h2>Casting LB-agar plates: AB<br>27 plates containing spectinomycin.<br><br><b>Preparation for sequencing:</b><br>69.1 pSB3K3-CP8-B0032-BFP<br>71.3 pSB3K3-CP29-B0032-BFP<br>72.3 pSB3K3-CP30-B0032-BFP<br><br><b>Dilution of primers: </b><br>200 µl VF2 (5 µM)<br>200 µl VR (5 µM)<br></div>';
+ }
+ else if(id == 'd2013711')
+ {
+  ds = '<div id="dairy-text"><br><h1>Thursday 2013-07-11</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Karl H, Peter C, Lovisa P, Theodor L, Ken B-A, Christoffer Ahlström (CA),  Anton Berglund (AB), Stephanie Herman (SH), Alona Nyberg (AN), Viktor Törnblom (VT), Viktor Blomkvist (VB), Mikael Strandgren (MS), Niclas, Sabri , Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α): </b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>96. Cs42s <br><br><b>L. plantarum:</b><br>10. 256-p256<br><br><h2>Todays work</h2><br><b>Transformation:</b> <br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>77. pSB4C15-red*<br>75. pEL3A15 - CrtB<br>76 pEL3K16 - CrtI<br>78. pEL3C18 - CrtY<br><br><b>Restreaks:</b> <br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>Lb10.3 plantarum 256 p256 *<br>Lb10.4 plantarum 256 p256<br>Lb10.5 plantarum 256 p256 *<br>Lb10.6 plantarum 256 p256<br><br><b>PCR:</b><br>77.(1-4) pSB4C15-red*<br><i>*Two different amplifications of plasmid adding restriction-sites with different primers.<br> One product with no removals and one without ori.</i><br><br><b>PCR-amplification:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>Digest:</b><br>14  pET13b(amp)-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br><i>-from plasmid prep with Pst1.</i><br><br><b>PCR screening & gel extraction:</b><br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br><br><b>Ligation: </b><br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br><br><b>Plasmid preparation:</b><br>77. pSB4C15-red<br><br>PCR screening of cs42s with RedTaq. Overnight culture on transfrormed 96.Cs42s.<br>Prepare of Vf2 and VR primer solution, 20x. 5 tubes each with 200 microliter/tube.<br><br><b>Subcloning of the following CrtB, CrtI, CrtY</b><br><br>23. pSB1C3-CrtB → pEL3A15 - red<br>24. pSB1C3-CrtI → pEL3K16 - red<br>25. CrtY(PCR prod.) → pEL3C18 - red<br><br><h2>Results</h2><br><b>Plasmid preparation: </b><br>77.1  pSB4C15-red: 47.2 ng/µl<br>77.2  pSB4C15-red: 50.2 ng/µl<br>77.3  pSB4C15-red: 44.0 ng/µl<br>77.4  pSB4C15-red: 54.4 ng/µl<br><br><b>Screening:</b><br>didn’t look good, the pcr product was approximatley 1000 bp on the gel.<br> Should have been around 4000.<br><br><h2>Other experiments</h2><br><b>Sequence verification:</b><br>61.1 pSB1C3-CP1<br>63.1 pSB1C3-CP11<br>67.1 pSB1C3-Cp44<br><br><b>Casting LB-agar plates: </b><br>26 plates containing spectinomycin.<br><br><b>Preparation of LB-agar solution: </b><br><b>transformation from kit:</b><br>90. BBa_J61002 (amp)-J23101<br>91. BBa_J61002 (amp)-J23106<br>92. BBa_J61002 (amp)-J23110<br>93. BBa_J61002 (amp)-J23116<br><br><b>Restreek from stock:</b><br>2012 SD(Strain Database) 13 and 61(promotors).<br>Aliquoting VF2- and VR-primers.<br><br></div>';
+ }
+ else if(id == 'd2013710')
+ {
+ ds = '<div id="dairy-text"><br><h1>Wednesday 2013-07-10</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Karl H, Peter C., Lovisa P, Ken B-A, Alexander, Niclas, Magnus, Nils, Thorsteinn, Christoffer Ahlström (CA),  Anton Berglund (AB), Stephanie Herman (SH), Viktor Törnblom (VT)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>96. p?A?-Cs42s<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><h2>Todays work</h2><br><b>Digestion:</b> <br>22. pSB1C3-RBS-B0032-BFP<br><br><b>Ligation:</b><br>44. pSB1C3-B0034-His-TAL<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>transformation:</b><br>44. pSB1C3-B0034-His-TAL<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>86. pSB3K3-CP44-B0032-BFP<br>87. pSB3K3-CP1-B0032-BFP<br>88. pSB3K3-CP41-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Re-streak</b> <br>96. p?A?-cs42s<br><i>-Streak of transformations of ligated 20 and positive controll (AV,NA)<br>PCR of 19(digested EP), 20(digested EP), 21(digested EP) and CrtO(from 21digestEP) (AV,NA)</i><br><br><b>Spreading of strains: </b><br>Lb4. reuteri 100-23 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb10. plantarum 256 p256<br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 noplasmid: Growth<br>Lb11. plantarum 36E noplasmid: Growth<br>→ Spread out (1x) on erythromycin plates, to examine whether they are naturally resistant.<br><br><h2>Results</h2><br><b>Transformation: </b> <br>Lb4. reuteri 100-23 noplasmid: Growth <br>Lb12. reuteri DSM 20016 noplasmid: Growth<br><br><b>Re-streak:</b> <br>96. p?A?-cs42s worked well. <br><br><b>O/N culture:</b><br>69.1 pSB3K3-CP8-B0032-BFP <br>69.2 pSB3K3-CP8-B0032-BFP <br>69.3 pSB3K3-CP8-B0032-BFP <br>69.4 pSB3K3-CP8-B0032-BFP <br>70.1 pSB3K3-CP11-B0032-BFP <br>70.2 pSB3K3-CP11-B0032-BFP <br>70.3 pSB3K3-CP11-B0032-BFP <br>70.4 pSB3K3-CP11-B0032-BFP <br>71.1 pSB3K3-CP29-B0032-BFP<br>71.2 pSB3K3-CP29-B0032-BFP<br>71.3 pSB3K3-CP29-B0032-BFP<br>71.4 pSB3K3-CP29-B0032-BFP<br>72.1 pSB3K3-CP30-B0032-BFP<br>72.2 pSB3K3-CP30-B0032-BFP<br>72.3 pSB3K3-CP30-B0032-BFP<br>72.4 pSB3K3-CP30-B0032-BFP<br>*Growth<br><br><b>Plasmid preparation: </b><br>69.1 pSB3K3-CP8-B0032-BFP: 80.5 ng/µl<br>69.2 pSB3K3-CP8-B0032-BFP: 75.7 ng/µl<br>69.3 pSB3K3-CP8-B0032-BFP: 60.5 ng/µl<br>69.4 pSB3K3-CP8-B0032-BFP: 50.8 ng/µl<br>70.1 pSB3K3-CP11-B0032-BFP: 71.7 ng/µl<br>70.2 pSB3K3-CP11-B0032-BFP: 56.3 ng/µl<br>70.3 pSB3K3-CP11-B0032-BFP: 56.0 ng/µl<br>70.4 pSB3K3-CP11-B0032-BFP: 45.3 ng/µl<br>71.1 pSB3K3-CP29-B0032-BFP: 54.0 ng/µl<br>71.2 pSB3K3-CP29-B0032-BFP: 49.1 ng/µl<br>71.3 pSB3K3-CP29-B0032-BFP: 83.0 ng/µl<br>71.4 pSB3K3-CP29-B0032-BFP 67.8 ng/µl<br>72.1 pSB3K3-CP30-B0032-BFP 53.8 ng/µl<br>72.2 pSB3K3-CP30-B0032-BFP 71.8 ng/µl<br>72.3 pSB3K3-CP30-B0032-BFP 109.6 ng/µl<br>72.4 pSB3K3-CP30-B0032-BFP 106.8 ng/µl<br></div>';
+ }
+ else if(id == 'd201379')
+ {
+  ds = '<div id="dairy-text"><br><h1>Tuesday 2013-07-09</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Lovisa P, Theodor L, Nils, Alexander, Christoffer, Niclas, Magnus, Thorsteinn, Christoffer Ahlström (CA), Stephanie Herman (SH), Nafisa Bashir (NB), Anton Berglund (AB), Mikael Strandgren (MS), Viktor Blomkvist (VB), Viktor Törnblom (VT)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α): </b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>96. p?-Cs42s<br>81. pSB4S15-CP41<br>83. pSB4S15-CP30<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB3K3-CP44<br>87. pSB3K3-CP1<br>88. pSB3K3-CP41<br>89. pSB4S15-CP44<br>90. pSB4S15-CP1<br>69. pSB3K3-CP8-B0032-BFP <br>70. pSB3K3-CP11-B0032-BFP <br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br><br><b>L. reuteri:</b><br>Lb4. 100-23-no plasmid<br>Lb12. DSM 20016-no plasmid<br>Lb10. 256-noplasmid<br>LB11. 36E-noplasmid<br><br><h2>Todays work</h2><br><b>Gel analysis:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br><br><b>Ligation:</b><br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br><br><b>OD600 measurement: </b><br>Lb4. reuteri 100-23 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br><br><b>Competent cells preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br><br><b>Transfromation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br><br><b>Re-streaks:</b><br>77. pSB4C15-red<br><br><b>Transformation:</b><br>81. pSB4S15-CP41<br>83. pSB4S15-CP30<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB3K3-CP44<br>87. pSB3K3-CP1<br>88. pSB3K3-CP41<br>89. pSB4S15-CP44<br>90. pSB4S15-CP1<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br><br><b>O/N culture:</b><br>69.1 pSB3K3-CP8-B0032-BFP <br>69.2 pSB3K3-CP8-B0032-BFP <br>69.3 pSB3K3-CP8-B0032-BFP <br>69.4 pSB3K3-CP8-B0032-BFP <br>70.1 pSB3K3-CP11-B0032-BFP <br>70.2 pSB3K3-CP11-B0032-BFP <br>70.3 pSB3K3-CP11-B0032-BFP <br>70.4 pSB3K3-CP11-B0032-BFP <br>71.1 pSB3K3-CP29-B0032-BFP<br>71.2 pSB3K3-CP29-B0032-BFP<br>71.3 pSB3K3-CP29-B0032-BFP<br>71.4 pSB3K3-CP29-B0032-BFP<br>72.1 pSB3K3-CP30-B0032-BFP<br>72.2 pSB3K3-CP30-B0032-BFP<br>72.3 pSB3K3-CP30-B0032-BFP<br>72.4 pSB3K3-CP30-B0032-BFP<br><br>Elution and transformation of 96. The transfrormed samples were plated (80μl of 1x and 200μl of 10x dilution) on all available resistances, as the backbone was unknown.<br>Ligation of 20 with all diffrent combinations of ligase buffer and ligase. (AV, CF, NA)<br>Transformation of ligation mentioned above, and a positive controll of 20 plasmidprepp. Although plates for streaking were missing so we couldn’t streak (CF, AV, NA)<br><br><h2>Results</h2><br><b>O/N:</b><br>Lb4. reuteri 100-23 no plasmid (clone 1): NC ok! → follow protocol for transformation<br>Lb12. reuteri DSM 20016 no plasmid (clone 1): NC ok! → follow protocol for transformation, watch in microscope for contamination<br>Lb10. plantarum 256 no plasmid (clone 1): NC ok! → follow protocol for transformation<br>Lb11. plantarum 36E no plasmid (clone 1): NC ok! → follow protocol for transformation<br><br><b>Transformation:</b><br>77. pSB4C15-red: Growth on Cm, some red colonies, some blue, many white (?!)<br><br><b>OD600 measurement:</b><br>Lb4. reuteri 100-23 no plasmid: 0.195 (Measure) & 0.668 (Cultivate) → proceed<br>Lb12. reuteri DSM 20016 no plasmid: 0.179 (Measure) & 0.680 (Cultivate)	--”--<br>Lb10. plantarum 256 no plasmid: 0.115 (Measure) & 0.724 (Cultivate)	--”--<br>Lb11. plantarum 36E no plasmid: 0.148 (Measure) & 0.624 (Cultivate)	--”--<br><br><b>Microscopy: </b><br>Lb12. reuteri DSM 20016 no plasmid: No contamination was sighted! → proceed!<br><br><b>transformation:</b><br>Successful:<br>45. pSB1C3-B0034-4CL<br>96. p?-Cs42s<br><br><b>failed:</b><br>44. pSB1C3-B0034-His-TAL<br><h2>Other experiments</h2>Cold buffer preparation (1 l) <br><br>50% glycerol stock preparation (100 ml)<br><br>Microscopy: Lb12. reuteri DSM 20016 no plasmid<br></div>';
+ }
+ else if(id == 'd201378')
+ {
+  ds = '<div id="dairy-text"><h1>Monday 2013-07-08</h1><br><b>Name of participants:</b> Alexander, Nils, Christofffferz , Magnus, Niclas, Thorsteinn, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Lovisa P<br><br><h2>Ongoing constructs</h2><br><b>E.coli (strain D5α): </b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0044-IspA<br>81. pSB4S15-CP41<br>83. pSB4S15-CP30<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB3K3-CP44<br>87. pSB3K3-CP1<br>88. pSB3K3-CP41<br>89. pSB4S15-CP44<br>90. pSB4S15-CP1<br><br><b>L. reuteri:</b><br>Lb4. 100-23-no plasmid<br>Lb12. DSM 20016-no plasmid<br><br><b>L. plantarum:</b><br>Lb10. 256-noplasmid<br>LB11. 36E-noplasmid<br><br><h2>Todays work</h2><br><b>PCR amplification and gel analysis:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br><br><b>Plasmid prep.:</b> <br>47. pSB1C3-B0034-STS<br>Clone: 47.9 and 47.20<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br><br><b>Sequencing prep.:</b><br>47. pSB1C3-B0034-STS <br>Clone: 47.9 and 47.20<br>48. pSB1C3-B0034-His-STS<br>Clone: 48.1<br><br><b>Gel extraction:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br><br><b>Restreak: </b><br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br><br><b>Digest</b><br>PCR products from succefull gel extraction.<br><br><b>Ligation: </b><br>81. pSB4S15-CP41<br>83. pSB4S15-CP30<br>84. pSB4S15-CP6<br>85. pSB4S15-CP25<br>86. pSB3K3-CP44<br>87. pSB3K3-CP1<br>88. pSB3K3-CP41<br>89. pSB4S15-CP44<br>90. pSB4S15-CP1<br><br><br><b>Frozen stock: </b><br>Lb4. reuteri 100-23 no plasmid (clone 1 & 2)<br>Lb12. reuteri DSM 20016 no plasmid (clone 1 & 2)<br>Lb10. plantarum 256 noplasmid (clone 1 & 2)<br>Lb11. plantarum 36E no plasmid (clone 1 & 2)<br><br><b>O/N:</b> <br>Lb4. reuteri 100-23 no plasmid (clone 1)<br>Lb12. reuteri DSM 20016 no plasmid (clone 1)<br>Lb10. plantarum 256 no plasmid (clone 1)<br>Lb11. plantarum 36E no plasmid (clone 1)<br><br><b>Screening:</b><br>43. pSB1C3-B0034-TAL<br><br><b>O/N culture:</b><br>43.7. pSB1C3-B0034-TAL<br><br><b>Subcloning:</b><br>23->4, 24->4, 25->4(kontroll), 23->38, 24->27, 25->51 (CF, AV)<br>Transformation of 23->4, 24->4, 25->4(kontroll), 23->38, 24->27, 25->51 (CF, AV)<br>Primer design and order of lambda red (NA)<br>Sequencing preparation of 57.3 and 57.5 (pSB1C3-B0034-Idi), 58.1 and 58.4 (pSB1C3-B0034-IspA)<br><br><h2>Results</h2><br><b>Microscopy:</b> *<br><i>*No contamination was seen, save plates for later. → Proceed with O/N of two clones from the no-plasmid strains.</i> <br><br>Primers for lambda red and Cs42s were ordered today :D.<br>Transformation of 23->4(blank), 24->4(blank), 25->4(blank), 23->38(blank), 24->27(a few clones, both red and white on x1), 25->51(blank)<br><br><b>PCR amplification:</b><br>43-46 failed, most likely primer dimers - retry with different concentrations of template DNA, higher concentration of DMSO and a colony PCR of each construct.<br><br><b>gel extraction: </b><br><b>Successfull </b><br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br><br><b>failed</b><br>43. pSB1C3-B0034-STS<br>46. pSB1C3-B0034-His-4CL<br><br><b>Screening:</b><br>one succefull clone - 43.7 pSB1C3-B0034-STS<br><br><h2>Other experiments</h2><br><b>Microscopy:</b> CA*<br>*Of plates from 04/07 and O/N from 07/07 to see if contaminated. </div>';
+ }
+else if(id == 'd201377')
+ {
+  ds = '<div id="dairy-text"><h1>Sunday 2013-07-07</h1><br><b>Name of participants:</b>Anders Edlund (AE)<br><br><h2>Ongoing constructs</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>77. pSB4S15-C-resistance<br><br><h2>Todays work</h2><br><b>Transformation (AE)</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>77. pSB4S15-C-resistance<br></div>';
+ }
+ else if(id == 'd201376')
+ {
+  ds = '<div id="dairy-text"><h1>Saturday 2013-07-06</h1><br><b>Name of participants:</b> Stephanie Herman (SH), Anders Edlund (AE), Nafisa Bashir (NB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Todays work</h2><br><b>Plasmid Preparation:</b> <br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br><br><b>Transformation: </b><br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br><h2>Results</h2><br><b>Previous day:</b><br>The O/N on the nonplasmids 4, 10, 11, 12 from the previous day had grown at a previously unexperienced rate. A contamination of the original plates from Stefan Roos was suspected as the NC was clear. The O/N was put in the cold room awaiting examination by microscope.<br><br><b>Today:</b><br><b>Plasmid Preparation:</b><br>42. pSB4S15-red<br>61. pSB1C3-CP1: (1) 203.2, (2) 202.9<br>62. pSB1C3-CP8: (1) 254.5, (2) 229.2<br>63. pSB1C3-CP11: (1) 165.0, (2) 260.1<br>64. pSB1C3-CP29: (1) 315.4, (2) 221.9<br>65. pSB1C3-CP30: (1) 243.1, (2) 101.5<br>66. pSB1C3-CP41: (1) 190.7, (2) 68.4<br>67. pSB1C3-Cp44: (1) 169.1, (2) 188.7<br></div>';
+ }
+ else if(id == 'd201375')
+ {
+  ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-CP44<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br><br><b>L. reuteri</b><br>Lb4. 100-23 no plasmid<br>Lb12. DSM 20016 no plasmid<br><br><b>L. plantarum:</b><br>Lb10. 256 no plasmid<br>Lb11. 36E no plasmid<br><br><b>L. thermophilus:</b><br>Lb19. L.thermophilus<br><br><b>Todays work</b><br>Screening PCR & gel analysis of constructs:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br><b>Plasmid prep & glycerol stock:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>O/N:</b> <br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>47.(9, 20) pSB1C3-B0034-STS<br>Lb4. reuteri 100-23 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Re-streaks:</b><br>Lb19. L.thermophilus<br><br><h2>Results</h2><br>The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB.<br><br><b>PCR screen and gel electrophoresis:</b><br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br>*The gel only showed smudgy bands.<br><br><b>succesful</b><br>47.9 pSB1C3-B0034-STS<br>47.20 pSB1C3-B0034-STS<br><br><b>failed</b><br>43. pSB1C3-B0034-TAL<br>46. pSB1C3-B0034-His-4CL<br><br>No contamination in negative control -_-<br>Religation from yesterday failed. No colonies, only a few at 76, both red and white. <br><br><h2>ther experiments</h2><br><b>Contamination control:</b> CA *<br><br><i>*Contamination control of growth medium for Lc.lactis and Lb.reuteri.<br> Two test from both bottles. </i><br><br><b>E.coli growth test:</b> CA *<br><br><i>*To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </i></div>';
+ }
+  else if(id == 'd201374')
+ {
+  ds = '<div id="dairy-text"><h1>Thursday 2013-07-04</h1><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Nafisa Bashir (NB), Mikael Strandgren (MS), Anton Berglund (AB),  Magnus, Thorsteinn, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs</h2><b>E.coli (strain D5α):</b> <br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>42. pSB4S15-red<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-CP44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br>Lb15. unknown-pGus<br><br><b>E. faecalis:</b><br>Lb9. JH2-2 pAMβ1<br><br><h2>Todays work</h2><b>Frozen stock and plasmid preparation:</b> <br>Lb9.7. faecalis JH2-2 pAMβ1 clone 7 (erythromycin) <br>Lb9.8. faecalis JH2-2 pAMβ1 clone 8 (no-antibiotic)<br>Lb9.9. faecalis JH2-2 pAMβ1 clone 9 (erythromycin)<br>Lb9.10. faecalis JH2-2 pAMβ1 clone 10 (no-antibiotic) <br>Lb13.5. Lc. lactis MG1363 pJP059 clone 5<br>Lb13.6. Lc. lactis MG1363 pJP059 clone 6<br>Lb13.7. Lc. lactis MG1363 pJP059 clone 7<br>Lb13.8. Lc. lactis MG1363 pJP059 clone 8<br>Lb15.5. Lc. lactis MG1363 pGus clone 5<br>Lb15.6. Lc. lactis MG1363 pGus clone 6<br>Lb15.7. Lc. lactis MG1363 pGus clone 7<br>Lb15.8. Lc. lactis MG1363 pGus clone 8<br><br><b>Touchdown PCR: </b><br>Lb13.2.2 Lc. lactis MG1363 pJP059<br>Lb13.2.3 Lc. lactis MG1363 pJP059<br>Lb13.3 Lc. lactis MG1363 pJP059<br>Lb13.4 Lc. lactis MG1363 pJP059<br><br><b>Screening PCR: </b><br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br><b>Gel electrophoresis:</b> <br>Lb13.2.2 lactis MG1363 pJP059 (PCR with DMSO done yesterday)<br>Lb13.2.3 lactis MG1363 pJP059 (PCR with DMSO done yesterday)<br>Lb13.3 lactis MG1363 pJP059 (PCR with DMSO done yesterday)<br>Lb13.4 Lc. lactis MG1363 pJP059 (PCR with DMSO done yesterday)<br>Lb13.2.2 Lc. lactis MG1363 pJP059 (Touchdown-PCR today)<br>Lb13.2.3 Lc. lactis MG1363 pJP059 (Touchdown-PCR today)<br>Lb13.3 Lc. lactis MG1363 pJP059 (Touchdown-PCR today)<br>Lb13.4 Lc. lactis MG1363 pJP059 (Touchdown-PCR today)<br>62.1 pSB1C3-CP8 (Screening-PCR today, was run two times)<br>62.2 pSB1C3-CP8 (Screening-PCR today, was run two times)<br>63.1 pSB1C3-CP11 (Screening-PCR today, was run two times)<br>63.2 pSB1C3-CP11 (Screening-PCR today, was run two times)<br>63.3 pSB1C3-CP11 (Screening-PCR today, was run two times)<br>63.4 pSB1C3-CP11 (Screening-PCR today, was run two times)<br>64.1.1 pSB1C3-CP29 (Screening-PCR today, was run two times)<br>64.1.2 pSB1C3-CP29 (Screening-PCR today, was run two times)<br>64.1.3 pSB1C3-CP29 (Screening-PCR today, was run two times)<br>64.1.4 pSB1C3-CP29 (Screening-PCR today, was run two times)<br>65.1.1 pSB1C3-CP30 (Screening-PCR today, was run two times)<br>65.1.2 pSB1C3-CP30 (Screening-PCR today, was run two times)<br>65.2.1 pSB1C3-CP30 (Screening-PCR today, was run two times)<br>65.2.2 pSB1C3-CP30 (Screening-PCR today, was run two times)<br>67.1.1 pSB1C3-CP44 (Screening-PCR today, was run two times)<br>67.1.2 pSB1C3-CP44 (Screening-PCR today, was run two times)<br>67.2.1 pSB1C3-CP44 (Screening-PCR today, was run two times)<br>67.2.2 pSB1C3-CP44 (Screening-PCR today, was run two times)<br>1. pSB1C3-red (digest used in transformation, cut with E and S)<br>7. pSB3K3-red (digest used in transformation, cut with E and P)<br>22. pSB1C3-B0032-BFP (digest used in transformation, cut with X and P)<br>42. pSB4S15 (digest used in transformation, cut with SalI and SacI)<br>pSB3C17-BFP (digest used in transformation, cut with SalI and SacI)<br><br><b>PCR purification: </b><br>13.2.2 Lc. lactis MG1363-pJP059<br><br><b>Re-streaks: </b><br>42. pSB4S15-red<br>61. pSB1C3-CP1, clone 1-8 (spread out 2/7)<br>62. pSB1C3-CP8, clone 4-8 (spread out yesterday)<br>63. pSB1C3-CP11, clone 4-8 (spread out yesterday)<br>64. pSB1C3-CP29, clone 4-8 (spread out yesterday)<br>65. pSB1C3-CP30, clone 4-8 (spread out yesterday)<br>66. pSB1C3-CP41, clone 1-8 (spread out 2/7)<br>67. pSB1C3-Cp44, clone 4-8 (spread out yesterday)<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>Lb1. reuteri CF48 pLR581<br>Lb2. reuteri 1063 pLUL631<br>Lb3. reuteri DSM 20016 pVS2<br>Lb4. reuteri 100-23 no plasmid<br>Lb6. reuteri DSM 20016 pLUL63TsA8<br>Lb7. reuteri DSM 20016 pGFP<br>Lb8. reuteri 100-23 pGT232<br>Lb12. reuteri DSM 20016 no plasmid<br>Lb14. reuteri DSM 20016 pLUL631(B?)<br>Lb5. plantarum 256 rifR pAMβ1<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br><br><b>Screening PCR and gel analysis:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>Overnight culture:</b><br>48. pSB1C3-B0034-His-STS<br>42. pSB4S15-red<br><br><b>Gel</b> of Crt Z<br>Ligation of 38(religation of digest), 27(religation of digest), 51(religation of digest), 75, 76, 78<br>Transformation of 38(religated), 38, 27(religated), 27, 51(religated), 51, 75, 76, 78<br>O/N of 57. and 58.<br><br><h2>Results</h2><br><b>Screening PCR and gel analysis:</b><br>48.1 pSB1C3-B0034-His-STS, was successful<br>45. pSB1C3-B0034-4CL<br>47. pSB1C3-B0034-STS, failed.<br><br><b>Gel</b> of CrtZ was blank. PCR fail. Think up a new method. <br>Transformation of 75, 76 and 78(from yesterday) failed. Redo with negative controll of both ligation(by religating the digested pEL-plasmids) and plasmids(from plasmidprepp).<br>Negative controll of O/N 57. and 58. contaminated.<br><br><b>O/N:</b> <br>Lb10.1. plantarum 256: NC ok, but weirdly looking pellets and supernatants. <br>Lb11.1. plantarum 36E: NC ok, but weirdly looking pellets and supernatants. <br>Lb12.1 reuteri DSM 20016: NC ok, but weirdly looking pellets and supernatants. <br>→ Look in microscope to see if contaminated. <br><br><b>Assembly and transformation:</b> <br>1. pSB1C3-red, as NC: Good growth<br>7. pSB3K3-red, as NC: No growth<br>42. pSB4S15-red, as NC: No growth<br>61. pSB1C3-CP1: Growth, discernable red and white colonies<br>62. pSB1C3-CP8: Growth, discernable red and white colonies<br>63. pSB1C3-CP11: Growth, discernable red and white colonies<br>64. pSB1C3-CP29: Growth, discernable red and white colonies<br>65. pSB1C3-CP30: Growth, discernable red and white colonies<br>66. pSB1C3-CP41: Growth, discernable red and white colonies<br>67. pSB1C3-Cp44: Growth, discernable red and white colonies<br>77 pSB4C15-red: Growth, but only white and blue colonies*<br>*E. coli with the right plasmid should have become red.<br>→ Most seem ok, do restreaks! Test fluorescence of BFP-colonies from 2/7, get new 42’s from Erik Gullberg since not even our frozen stocks are red (as they should be).<br><br><b>Gel electrophoresis:</b> <br>Lb13.2.2 lactis MG1363 pJP059 (yesterday)		No bands<br>Lb13.2.3 lactis MG1363 pJP059 (yesterday)		--”--<br>Lb13.3 lactis MG1363 pJP059 (yesterday)		--”--<br>Lb13.4 Lc. lactis MG1363 pJP059 (yesterday)		--”--<br>Lb13.2.2 Lc. lactis MG1363 pJP059 (Touchdown)	Right band (~2000)<br>Lb13.2.3 Lc. lactis MG1363 pJP059 (Touchdown)	No bands<br>Lb13.3 Lc. lactis MG1363 pJP059 (Touchdown)	--”--<br>Lb13.4 Lc. lactis MG1363 pJP059 (Touchdown)	--”--<br>62.1 pSB1C3-CP8 (Screening)				* (see below)<br>62.2 pSB1C3-CP8 (Screening)				* (see below)<br>63.1 pSB1C3-CP11 (Screening)				* (see below)<br>63.2 pSB1C3-CP11 (Screening)				* (see below)<br>63.3 pSB1C3-CP11 (Screening)				* (see below)<br>63.4 pSB1C3-CP11 (Screening)				* (see below)<br>64.1.1 pSB1C3-CP29 (Screening)			* (see below)<br>64.1.2 pSB1C3-CP29 (Screening)			* (see below)<br>64.1.3 pSB1C3-CP29 (Screening)			* (see below)<br>64.1.4 pSB1C3-CP29 (Screening)			* (see below)<br>65.1.1 pSB1C3-CP30 (Screening)			* (see below)<br>65.1.2 pSB1C3-CP30 (Screening)			* (see below)<br>65.2.1 pSB1C3-CP30 (Screening)			* (see below)<br>65.2.2 pSB1C3-CP30 (Screening)			* (see below)<br>67.1.1 pSB1C3-CP44 (Screening)			* (see below)<br>67.1.2 pSB1C3-CP44 (Screening)			* (see below)<br>67.2.1 pSB1C3-CP44 (Screening)			* (see below)<br>67.2.2 pSB1C3-CP44 (Screening)			* (see below)<br>1. pSB1C3-red (cut with E and S): 			Clear bands (safe to use)<br>7. pSB3K3-red (cut with E and P): 			Clear bands (safe to use)<br>22. pSB1C3-B0032-BFP (cut with X and P)		No bands (get more data)<br>42. pSB4S15 (cut with SalI and SacI)			No bands (get more data)<br>pSB3C17-BFP (cut with SalI and SacI)			Faint bands (likely safe)<br><br>*Neither of the two runs on gel showed anything but weak, smudgy bands. Try again.<br><br><b>PCR purification: </b><br>Lb13.2.2 Lc. lactis MG1363-pJP059: 25.9 ng/µl<br><br><b>Plasmid preparation: </b><br>Lb9.7.1 faecalis JH2-2 pAMβ1 clone 7.1 (erythromycin): 140.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.7.2 faecalis JH2-2 pAMβ1 clone 7.2 (erythromycin): 116.0 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.8.1 faecalis JH2-2 pAMβ1 clone 8.1 (no-antibiotic): 76.6 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.8.2 faecalis JH2-2 pAMβ1 clone 8.2 (no-antibiotic): 55.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.9.1 faecalis JH2-2 pAMβ1 clone 9.1 (erythromycin): 231.9 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.9.2 faecalis JH2-2 pAMβ1 clone 9.2 (erythromycin): 96.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.10.1 faecalis JH2-2 pAMβ1 clone 10.1 (no-antibiotic): 113.2 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb9.10.2 faecalis JH2-2 pAMβ1 clone 10.2 (no-antibiotic): 107.5 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb13.5. Lc. lactis MG1363 pJP059 clone 5: 13.8 ng/µl → Dispose & don’t save in freezer. <br>Lb13.6. Lc. lactis MG1363 pJP059 clone 6: 34.8 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb13.7. Lc. lactis MG1363 pJP059 clone 7: 25.9 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb13.8. Lc. lactis MG1363 pJP059 clone 8: 34.3 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb15.5. Lc. lactis MG1363 pGus clone 5: 5.9 ng/µl → Dispose & don’t save in freezer. <br>Lb15.6. Lc. lactis MG1363 pGus clone 6: 9.7 ng/µl → Dispose & don’t save in freezer. <br>Lb15.7. Lc. lactis MG1363 pGus clone 7: 15.7 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br>Lb15.8. Lc. lactis MG1363 pGus clone 8: 16.7 ng/µl → Throw away other low concentration clones and replace with this one in the -80o C freezer.<br><br><h2>Other experiments</h2>TBE-mix<br>Agarose gel mix<br>EDTA mix<br><br><b>Microscoping:</b> *To see if the overnights from yesterday on strains 10 → 12 were contaminated.<br>They showed to be that and the source are thought to be from a time ago, before frozen stock, since NC checked good for these overnights. </div>';
+ }
+ else if(id == 'd2013722')
+ {
+  ds = '<div id="dairy-text"><h1>Monday 2013-07-22</h1><br><b>Name of participants:</b> Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Nils, Christoffer, Malin, Magnus, Niclas, Pontus, Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Mikael Strandgren (MS)<br><br><h2>Ongoing constructs</h2><br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>57.pSB1C3-B0034-Idi<br>99. pSB1C3-CrtO<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>127. pSB1A3-B0034-UGTCs2-His<br>21. CrtE~I /w RBS, Zinc finger (PCR prod)<br>128. pSIM5-tet(Nissle)<br>130. pSB1C3-CrtE~I /w RBS, Zinc Finger<br>131. pSB4A15-J23101-CrtE~I /w RBS, Zinc Finger<br>132. pSB4A15-J23106-CrtE~I /w RBS, Zinc Finger<br>133. pSB4A15 -J23110-CrtE~I /w RBS, Zinc Finger<br>75 . pEL3A15 - CrtB (LR) <br>76.pEL3K16-CrtI (LR) <br>79.pEL3K16- CrtE (LR) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144.pSB1C3-B0034-UGTCs2-His<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>85. pSB4S15-CP25<br>84. pSB4S15-CP6<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>134.1 pSB1C3-CBP<br><br><h2>Todays work</h2><br><b>Restreaks:</b><br>115. pSB3T5-J23101-B0034-His-TAL*<br>116. pSB3T5-J23110-B0034-His-TAL*<br>117. pSB3T5-CP1-B0034-His-TAL*<br>137. pSB3K3-CP8-B0034-TAL*<br>138. pSB3K3-CP8-B0034-His-TAL*<br>139. pSB3K3-J23101-B0034-BFP*<br><i>-*Restreaks from the transformation, that was done the day before</i><br><br><b>Mutagenisis:</b><br>57.pSB1C3-B0034-Idi -> 129. pSB1C3-B0034-Idi (mut)<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br><i>→ Run PCR-products on gel to see if correct and then PCR clean it and prepare for sequencing. </i><br><br><b>Phosphorylation of primers: </b><br>usp45_mut_R<br>usp45_mut_F<br><br><b>Overnight:</b><br>127.1.pSB1A3-B0034-UGTCs2-His <br>127.2.pSB1A3-B0034-UGTCs2-His <br>86. pSB4S15-CP6-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15- CP11-B0032-BFP<br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br><br><b>Transformation: </b><br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>81. pSB4S15-CP41-B0032-BFP<br>82. pSB4S15-CP29-B0032-BFP<br>83. pSB4S15-CP30-B0032-BFP<br>85. pSB4S15-CP25<br><br><b>Fluorescence(BFP) experiment with UV:</b><br>84. pSB4S15-CP6<br>86. pSB4S15-CP6-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br><br><b>Screening:</b><br>131.  pSB4A15-BBa-J61002-J23106-CrtE~I /w RBS, Zinc finger <br>132.  pSB4A15-BBa-J61002-J23110-CrtE~I /w RBS, Zinc finger <br>133.  pSB4A15-BBa-J61002-J23101-CrtE~I /w RBS, Zinc finger<br>75.   pEL3A15 - CrtB <br>76.   pEL3K16- CrtI <br>79.   pEL3K16 - CrtE <br><br><b>Digest</b><br>3.pSB3T5-red<br>92.BBa_J61002-J23110<br>90.BBa_J61002-J23101<br>127.pSB1A3-B0034-UGTCs2-His<br>51.pEL3C18-CP1<br>51.pEL3C18-CP8<br>58.pSB1C3-B0034-IspA<br>11.pSB4A15-red<br>91.BBa_J61002-J23106<br>94.pSB1C3-K206000<br>1.pSB1C3-red<br>CSP_3<br>Plasmid 1.1 (from strain database 2013)<br><br><b>Ligation:</b><br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002-J23110 --> 140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002-J23101 --> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 51.pEL3C18-CP1       --> 142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 51.pEL3C18-CP8       --> 143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 91.BBa_J61002-J23106      --> 108.pSBA15-J23106-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000         --> 109.pSBA15-K206000-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001         --> 110.pSBA15-K206001-B0034-IspA<br>127.pSB1A3-B0034-UGTCs2-His, 1.pSB1C3-red                       --> 144.pSB1C3-B0034-UGTCs2-His<br>CSP_3 <i>-Ligated with strain 1.1 (from strain database 2013) <br>    Let ligate in room temperature overnight.</i> <br>	<br><b>Frozen stock: (plasmid prep. tomorrow)</b><br>111.6 pSBLb4C15<br>111.7 pSBLb4C15<br><br><h2>Results</h2><br><b>Gel electrophoresis: </b><br>79.pEL3K16 - CrtE looked ok on gel, but there was primer dimer contamination. <br>75.pEL3A15 - CrtB had several bands around 1500-3000. <br>76.pEL3K16-CrtI displayed no band except primer dimers. <br>Digests looks good on gel<br><br><b>Overnights </b><br>127.1* pSB1A3-B0034-UGTCs2-His<br>127.2* pSB1A3-B0034-UGTCs2-His<br><i>-*did not grow.</i></div>';
+ }
+ else if(id == 'd2013723')
+ {
+  ds = '<div id="dairy-text"><h1>Tuesday 2013-07-23</h1><br><b>Name of participants:</b> Nils, Christoffer, Malin, Magnus, Niclas, Pontus, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Christoffer Ahlström (CA), Nafisa Bashir (NB), Mikael Strandgren (MS), Stephanie Herman (SH), Alona Nyberg (AN)<br><br><h2>Ongoing constructs:</h2><br>129. pSB1C3-B0034-Idi (mut)<br>108. pSB4A15-J23106-B0034-IspA<br>109. pSB4A15-K206000-B0034-IspA<br>110. pSB4A15-K206001-B0034-IspA<br>127. pSB1A3-B0034-UGTCs2-His<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144.pSB1C3-B0034-UGTCs2-His<br>147. pSB1C3-B0034-Thioredoxin<br>152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>75.pEL3A15 - CrtB (LR) <br>76.pEL3K16-CrtI (LR) <br>79.pEL3K16 - CrtE (LR) <br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>134. pSB1C3-CBP<br>111. pSBLb4C15<br>86. pSB4S15-CP6-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>136. pSB1C3-usp45<br><br>Lb9. faecalis JH2-2-pAMβ1<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>86. pSB4S15-CP6-B0032-BFP<br>97. pSB4S15-CP8-B0032-BFP<br>98. pSB4S15-CP11-B0032-BFP<br>111.6 pSBLb4C15<br>111.7 pSBLb4C15<br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br>84. pSB4S15-CP6<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><br><b>Mutagenesis:</b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>PCR:</b><br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>Lb9. faecalis JH2-2-pAMβ1 clone 10.1*<br>Lb9. faecalis JH2-2-pAMβ1 clone 10.2*<br><i>-*With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R. To isolate ori.</i><br><br><b>Digest:</b><br>B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin<br><br><b>Ligation:</b><br>pSB1C3-B0034-Thioredoxin<br><br><b>Restreak: </b><br>139. pSB3K3-J23101-B0032-BFP<br><br><b>Transformation:</b><br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144.pSB1C3-B0034-UGTCs2-His<br>152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>135. pSB1C3-CSP*<br>148. pSB1C3-gfas purple<br>149. pSB1C3-meffred<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br><i>-*Transformed into D5(alpha) competent cells. </i><br><br><b>Gel electrophoresis: </b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br><br><b>Screening:</b><br>129. pSB1C3-B0034-Idi (mut)<br>75.pEL3A15 - CrtE <br>76.pEL3K16 - CrtI <br>79.pEL3K16 - CrtB <br><br><b>Overnight:</b><br>127.1.pSB1A3-B0034-UGTCs2-His <br>127.2.pSB1A3-B0034-UGTCs2-His<br>84. pSB4S15-CP6<br>87. pSB4S15-CP25-B0032-BFP<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br><br><b>Spreading of strains:</b><br>135. pSB1C3-CSP*<br><i>-*Two 1x dilutions were performed.</i><br><br><b>Phosphorylation of primers: </b><br>usp45_mut_R<br>usp45_mut_F<br><br><b>Frozen stock: </b><br>134.1 pSB1C3-CBP<br>134.2 pSB1C3-CBP<br>134.3 pSB1C3-CBP<br>134.4 pSB1C3-CBP<br><br><h2>Results</h2><br><b>Previous day: </b><br><b>O/N: </b><br>134.1 pSB1C3-CBP: NC ok!<br>134.2 pSB1C3-CBP: NC ok!<br>134.3 pSB1C3-CBP: NC ok!<br>134.4 pSB1C3-CBP: NC ok!<br><br><b>Today:</b><br><b>Gel electrophoresis: </b><br>136.1 pSB1C3-usp45: Possible primer-dimer!<br>136.2 pSB1C3-usp45: Possible primer-dimer!<br>136.3 pSB1C3-usp45: Possible primer-dimer!<br><i>→ Re-do PCR mutagenesis with some edits.</i><br>129. pSB1C3-B0034-Idi  (mut) did not show on gel<br>76.pEL3K16-CrtI displayed no band on gel. <br>75.pEL3A15 - CrtB displayed a band, lager than expected. <br>79.pEL3K16 - CrtE displayed a band at the right size (screened without dmso) and a good band with some contamination when screened with 7% dmso. <br><br><b>Plasmid preparation: </b><br>134.1 pSB1C3-CBP: 126.0 ng/µl → Do a sequencing preparation.<br>134.2 pSB1C3-CBP: 124.9 ng/µl<br>134.3 pSB1C3-CBP: 126.6 ng/µl<br>134.4 pSB1C3-CBP: 126.2 ng/µl<br>111.6 pSBLb4C15: 33.7 ng/µl<br>111.7 pSBLb4C15: 52.2 ng/µl<br><br><b>Transformation: </b><br>109.pSBA15-K206000-B0034-IspA - did not grow<br>147. pSB1C3-B0034-Thioredoxin - did not grow<br><br><h2>Other experiments</h2><br>10x TBE-Buffer<br>10% Glycerol solution<br><br><b>Sequencing preparation:</b><br>111.6. pSBLb4C15-red<br>111.7. pSBLb4C15-red<br>134.1 pSB1C3-CBP<br><br><b>Primer dilution (from 100 µM to 5 µM):</b><br>Ori-scr_F<br>pL1-scr_F2<br>pL1-scr_F3<br>pL3-scr_F2<br>pL3-scr_F3<br>pAM-M1_F<br>pAM-s-X1_R<br>pAM-d-M1_R</div>';
+ }
+ else if(id == 'd2013724')
+ {
+  ds = '<div id="dairy-text"><h1>Wednesday 2013-07-24</h1><br><b>Name of participants:</b> Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Nafisa Bashir (NB), Mikael Strandgren (MS), Jens Isaksson (JI), Malin, Magnus, Pontus, Nils, Niclas, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H.<br><br><h2>Ongoing constructs:</h2><br>127.1.pSB1A3-B0034-UGTCs2-His <br>127.2.pSB1A3-B0034-UGTCs2-His<br>108. pSBA15-J23106-B0034-IspA<br>110. pSBA15-K206001-B0034-IspA<br>140. pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141. pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142. pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144. pSB1C3-B0034-UGTCs2-His<br>152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>90. BBa_J61002-J23101<br>153. pSB1C3-J23110-meffBlue<br>154. pSB1C3-J23106-meffBlue<br>155. pSB1C3-J23110-meffRed<br>156. pSB1C3-J23106-meffRed<br>157. pSB1C3-J23110-spisPink<br>158. pSB1C3-J23106-spisPink<br>159. pSB1C3-J23110-gfasPurple<br>160. pSB1C3-J23106-gfasPurple<br>66. pSB1C3-CP41<br>98. pSB4S15-CP11-B0032-BFP<br>22. pSB1C3-B0032-BFP<br>87. pSB4S15-CP25-B0032-BFP<br>84. pSB4S15-CP6<br>88. pSB4S15-CP1-B0032-BFP<br>89. pSB4S15-CP44-B0032-BFP<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-HIS-4CL<br>121. pSB1C3-B0034-STS<br>121. pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br><br><h2>Todays work</h2><br><b>Overnight culture: </b><br>68. pSB3K3-CP1-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br><br><b>Ligation:</b><br>139. pSB3K3-J23101-B0032-BFP<br>162. pSB1C3-J23101<br>B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin<br><br>125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br><br><b>Lambda Red:</b><br>164.E-coli Nissle-CrtY<br><br><b>Transformation: </b><br>90. BBa_J61002-J23101<br>153. pSB1C3-J23110-meffBlue<br>154. pSB1C3-J23106-meffBlue<br>155. pSB1C3-J23110-meffRed<br>156. pSB1C3-J23106-meffRed<br>157. pSB1C3-J23110-spisPink<br>158. pSB1C3-J23106-spisPink<br>159. pSB1C3-J23110-gfasPurple<br>160. pSB1C3-J23106-gfasPurple<br>147. pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br><br><b>Sequencing preparation: </b><br>66. pSB1C3-CP41<br>98. pSB4S15-CP11-B0032-BFP<br>127.1.pSB1A3-B0034-UGTCs2-His <br><br><b>Digestion: </b><br>22. pSB1C3-B0032-BFP<br>90. BBa_J61002(amp)-J23101<br>125.1 pSB1C3-ZCD-His<br><br><b>Gel electrophoresis: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>Re-streak:</b><br>108. pSBA15-J23106-B0034-IspA<br>110. pSBA15-K206001-B0034-IspA<br>140. pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141. pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142. pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144. pSB1C3-B0034-UGTCs2-His<br>152. pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His <br>135.1 pSB1C3-CSP<br>135.2 pSB1C3-CSP<br>135.3 pSB1C3-CSP<br>135.4 pSB1C3-CSP<br><br><b>Mutagenesis: </b><br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><br><b>PCR: </b><br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1<br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2<br><i>-New temperatures (-3*C), new polymerase and new primer dilutions. With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R. To isolate ori.</i><br><br><b>Plasmid Prep: </b><br>84.1 pSB4S15-CP6<br>84.2 pSB4S15-CP6<br>87.1 pSB4S15-CP25-B0032-BFP<br>87.2 pSB4S15-CP25-B0032-BFP<br>88.1 pSB4S15-CP1-B0032-BFP<br>88.2 pSB4S15-CP1-B0032-BFP<br>89.1 pSB4S15-CP44-B0032-BFP<br>89.2 pSB4S15-CP44-B0032-BFP<br>127.1.pSB1A3-B0034-UGTCs2-His <br>127.2.pSB1A3-B0034-UGTCs2-His<br><br><b>Screening PCR and gel electrophoresis on:</b><br>115. pSB3T5-J23101-B0034-His-TAL clone: 1, 2, 3 ,4<br>116. pSB3T5-J23110-B0034-His-TAL clone: 1, 2, 3, 4<br>117. pSB3T5-CP1-B0034-His-TAL clone: 1, 2, 3, 4<br>137. pSB3K3-CP8-B0034-TAL clone: 1, 2, 3, 4<br>138. pSB3K3-CP8-B0034-His-TAL clone: 1, 2, 3, 4<br>139. pSB3K3-J23101-B0034-BFP clone: 1, 2, 3, 4<br>Sequence prep on: <br>116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4<br>137. pSB3K3-CP8-B0034-TAL clone: 1, 4<br>Overnight culture on: <br>116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4<br>137. pSB3K3-CP8-B0034-TAL: 1, 4<br>Measurement of standards of p-coumaric acid in the shimadzu UV160, plotting of a standard graph.<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>Spreading of strains: </b><br>135. pSB1C3-CSP: Both white and red colonies.<br><i>*Two 1x dilutions were performed.<br>→ Re-streak with 4 colonies.</i><br><br><b>PCR:</b> <br>One of the PCR-machines (with 2x MluI-pAM)  <br><i>-were accidentally paused right after start last night, and so it had to be re-done this morning. Since the samples stood in room temperature for about 15 hour the polymerase may have lost it’s activity, a gel will tell.</i><br><br><b>Today:</b><br><b>Gel electrophoresis: </b><br>Lb9.10.1 faecalis   JH2-2 pAMβ1 with XbaI and MluI: Not even primer-bands<br>Lb9.10.2 faecalis   JH2-2 pAMβ1 with 2x MluI: -”-<br>Lb9.10.1 faecalis   JH2-2 pAMβ1 with XbaI and MluI: -”-<br>Lb9.10.2 faecalis   JH2-2 pAMβ1 with 2x MluI: -”-<br>136.1 pSB1C3-usp45: Possible primer-dimer and a mark at ~1000bp.<br>136.2 pSB1C3-usp45: Possible primer-dimer.<br>136.3 pSB1C3-usp45: Possible primer-dimer.<br>136.4 pSB1C3-usp45: Possible primer-dimer.<br><i>→ Re-do PCR with other temperatures!</i><br><br><b>Plasmid Prepp: </b><br>84.1 pSB4S15-CP6:            59.3 ng/µl<br>84.2 pSB4S15-CP6:            66.7 ng/µl<br>87.1 pSB4S15-CP25-B0032-BFP: 12.6 ng/µl                                               	<br>87.2 pSB4S15-CP25-B0032-BFP: 52.0 ng/µl<br>88.1 pSB4S15-CP1-B0032-BFP:  508.2 ng/µl    	<br>88.2 pSB4S15-CP1-B0032-BFP:  489.7 ng/µl<br>89. pSB4S15-CP44-B0032-BFP:  7.4 ng/µl<br><br><b>digest</b> of B0034-Thioredoxin (Synthetic construct) looks good on gel<br><br><b>Restreak</b> of 143. pSB3T5-CP8-B0034-UGTCs2-6xHis did not grow<br><br><b>ligation</b> of 141. pSB3T5-J23101-B0034-UGTCs2-6xHis is wrong, since 92.BBa_J61002-J23101 was digested wrong<br><br>Construct 116.3, 116.4, 137.1, 137.4 successfull.<br><br><h2>Other experiments</h2><br><b>Primer dilutions (from 100µM to 5 µM): </b><br>pAM-M1_F<br>pAM-M1_R<br>pAM-X1_R<br></div>';
+ }
+ else if(id == 'd2013725')
+ {
+  ds = '<div id="dairy-text"><h1>Thursday 2013-07-25</h1><br><b>Name of participants:</b> Malin, Magnus, Niclas, Pontus, Nils, Nafisa Bashir (NB), Viktor Blomkvist (VB), Jens Isaksson (JI), Mikael Strandgren (MS), Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>108.pSBA15-J23106-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis <br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144.pSB1C3-B0034-UGTCs2-His<br>147.pSB1C3-B0034-Thioredoxin<br>152.pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>164.E-coli Nissle-CrtY<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br>135. pSB1C3-CSP<br>136.1 pSB1C3-usp45<br>148. pSB1C3-gfas purple<br>149. pSB1C3-meffred<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br>162. pSB1C3-J23101<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>E. faecalis:</b><br>9. JH2-2-pAMβ1 clone<br><br><h2>Todays work</h2><br><b>O/N:</b> <br>135. pSB1C3-CSP<br><br><b>Transformation:</b><br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis<br>162. pSB1C3-J23101<br><br><b>Restreak:</b><br>139. pSB3K3-J23101-B0032-BFP<br>148. pSB1C3-gfas purple<br>149. pSB1C3-meffred<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br>147. pSB1C3-B0034-Thioredoxin<br>164.E-coli Nissle-CrtY<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br><br><b>Plasmid prep on:</b><br>116. pSB3T5-J23110-B0034-His-TAL clone: 3, 4<br>137. pSB3K3-CP8-B0034-TAL clone: 1, 4<br><br><br><b>Gel electrophoresis of PCR products: </b><br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1*<br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2*<br>136.1 pSB1C3-usp45<br>136.2 pSB1C3-usp45<br>136.3 pSB1C3-usp45<br>136.4 pSB1C3-usp45<br><i>-*(With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R)</i><br><br><b>PCR-purification: </b><br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1*<br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2*<br><i>*With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R</i><br><br><b>Screening:</b><br>108.3 pSBA15-J23106-B0034-IspA<br>108.4 pSBA15-J23106-B0034-IspA<br>110.1 pSBA15-K206001-B0034-IspA<br>140.1 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.2 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.3 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.4 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>142.1 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.2 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.3 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.4 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>144.1 pSB1C3-B0034-UGTCs2-His<br>144.2 pSB1C3-B0034-UGTCs2-His<br>144.3 pSB1C3-B0034-UGTCs2-His<br>144.4 pSB1C3-B0034-UGTCs2-His<br><br><b>Overnight:</b><br>144.1 pSB1C3-B0034-UGTCs2-His<br>144.2 pSB1C3-B0034-UGTCs2-His<br>144.3 pSB1C3-B0034-UGTCs2-His<br>144.4 pSB1C3-B0034-UGTCs2-His<br>152.1 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.2 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.3 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.4 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Ligation:</b><br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 103.pSB1K3-J23101--> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis <br><br><b>Digest and assembly:</b><br>pL3E1 (pAMβ1 double)<br>pL3 (pAMβ1 single)<br>105. pSB3T5-J23101-B0034-TAL<br>115. pSB3T5-J23101-B0034--His-TAL<br><br><b>Transformation on construct:</b><br>105. pSB3T5-J23101-B0034-TAL<br>115. pSB3T5-J23101-B0034--His-TAL<br><br><h2>Results</h2><br><b>Screening of all clones failed except for:</b><br>144.1 pSB1C3-B0034-UGTCs2-His<br>144.2 pSB1C3-B0034-UGTCs2-His<br>144.3 pSB1C3-B0034-UGTCs2-His<br>144.4 pSB1C3-B0034-UGTCs2-His<br><br><b>Today:</b><br><b>Gel electrophoresis: </b><br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.1: Clear and correct bands, at ~1700 bp.*<br>Lb9. faecalis JH2-2-pAMβ1 clone 9.9.2: Clear and correct bands, at ~1700 bp.*<br>136.1 pSB1C3-usp45: Big smudge between approx. 2500 and 200 bp<br>136.2 pSB1C3-usp45: Empty<br>136.3 pSB1C3-usp45: Empty<br>136.4 pSB1C3-usp45: Empty<br> <i>*(With primers pAM-M1_F, pAM-s-X1_R and pAM-d-M1_R)</i><br><br><h2>Other experiments</h2><br>75. Digest to check for wrong bindings<br>Making LB-agar plates<br><br>Spectrophotomertry of 106.5, 106.6 and 43.7, using the shimadzu UV160.<br><i>-Inconclusive spectrophotometry results.</i></div>';
+ }
+ else if(id == 'd2013726')
+ {
+  ds = '<div id="dairy-text"><h1>Friday 2013-07-26</h1><br><b>Name of participants:</b> Nafisa Bashir (NB), Christoffer Ahlström (CA), Stephanie Herman (SH), Alona Nyberg (AN), Mikael Strandgren (MS), Viktor Blomkvist (VB), Jens Isaksson (JI), Malin, Magnus, Niclas, Pontus, Nils, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L<br><br><h2>Ongoing constructs</h2><br>148. pSB1C3-gfas purple<br>149. pSB1C3-meffred<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br>90. BBa_J61002-J23101<br>153. pSB1C3-J23110-meffBlue<br>154. pSB1C3-J23106-meffBlue<br>155. pSB1C3-J23110-meffRed<br>156. pSB1C3-J23106-meffRed<br>157. pSB1C3-J23110-spisPink<br>158. pSB1C3-J23106-spisPink<br>159. pSB1C3-J23110-gfasPurple<br>160. pSB1C3-J23106-gfasPurple<br>162. pSB1C3-J23101<br>168. pSBLbC2 (working name)<br>169. pSBLbEcC2 (working name)<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis<br>144.pSB1C3-B0034-UGTCs2-His<br>152.pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>147. pSB1C3-B0034-Thioredoxin<br>164.E-coli Nissle-CrtY<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br><br><h2>Todays work</h2><br>Screening:<br>108.3 pSBA15-J23106-B0034-IspA<br>108.4 pSBA15-J23106-B0034-IspA<br>110.1 pSBA15-K206001-B0034-IspA<br>140.1 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.2 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.3 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>140.4 pSB3T5-J23110-B0034-UGTCs2-6xHis<br>142.1 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.2 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.3 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>142.4 pSB3T5-CP1-B0034-UGTCs2-6xHis<br>147. pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br><br></b>Re-streak: </b><br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis<br>105. pSB3T5-J23101-B0034-TAL<br>115. pSB3T5-J23101-B0034--His-TAL<br><br>Plasmid prep:<br>144.1 pSB1C3-B0034-UGTCs2-His<br>144.2 pSB1C3-B0034-UGTCs2-His<br>144.3 pSB1C3-B0034-UGTCs2-His<br>144.4 pSB1C3-B0034-UGTCs2-His<br>152.1 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.2 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.3 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br>152.4 pUC57 (amp)-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Transformation*:</b><br>168. pSBLbC2 (ori from pSB4C15 replaced with ori from pAMβ1)<br>169. pSBLbEcC2 (ori from pAMβ1 added to pSB4C15)<br><i>* Not spread.</i><br><br><b>Ligation:</b><br>3.pSB3T5-red, 127.pSB1A3-B0034-UGTCs2-His, 103.pSB1K3-J23101--> 141.pSB3T5-J23101-B0034-UGTCs2-6xHis <br><br><h2>Results:</h2><br>Screening showed incorrect results on all constructs, but possible sources for error in the analysis made these results inconclusive. A new screening was set to run overnight.<br><br><h2>Other experiments</h2><br>Organization of frozen stock<br><br>75. Digest to check for wrong bindings<br><br>Two phase separation of p-coumaric acid with heptane. Spectrophotometry.<br><i>--Heptane proved not to be so good for the separation.</i></div>';
+ }
+ else if(id == 'd2013727')
+ {
+  ds = '<div id="dairy-text"><h1>Saturday 2013-07-27</h1><br><b>Name of participants:</b> Kristoffer L, Marcus H., Stephanie Herman (SH), Nafisa Bashir (NB), Malin, Magnus, Pontus, Nils<br><br><h2>Ongoing constructs</h2><br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>90. BBa_J61002-J23101<br>148. pSB1C3-gfas purple<br>149. pSB1C3-meffred<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br>153. pSB1C3-J23110-meffBlue<br>154. pSB1C3-J23106-meffBlue<br>155. pSB1C3-J23110-meffRed<br>156. pSB1C3-J23106-meffRed<br>157. pSB1C3-J23110-spisPink<br>158. pSB1C3-J23106-spisPink<br>159. pSB1C3-J23110-gfasPurple<br>160. pSB1C3-J23106-gfasPurple<br>162. pSB1C3-J23101<br><br><h2>Todays work</h2><br><b>Digest on construct:</b><br>106. pSB3T5-J23110-B0034-TAL<br>104. pSB1C3-B0034-4CL-B0034-STS<br><br><b>Assembly on cnstruct:</b><br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>Transformation on construct:<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br><br><b>Plasmid Preperation: </b><br>148.1 pSB1C3-gfas purple<br>148.2 pSB1C3-gfas purple<br>149.1 pSB1C3-meffred<br>149.4 pSB1C3-meffred<br>150.1 pSB1C3-spispink<br>150.2 pSB1C3-spispink<br>151.2 pSB1C3-meffblue<br><br><b>Overnight culture:</b> <br>90. BBa_J61002-J23101<br>153. pSB1C3-J23110-meffBlue<br>154. pSB1C3-J23106-meffBlue<br>155. pSB1C3-J23110-meffRed<br>156. pSB1C3-J23106-meffRed<br>157. pSB1C3-J23110-spisPink<br>158. pSB1C3-J23106-spisPink<br>159. pSB1C3-J23110-gfasPurple<br>160. pSB1C3-J23106-gfasPurple<br>162. pSB1C3-J23101<br><br><h2>Result</h2><br><b>Transformation on construct:</b><br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>Failed. Retry assembly<br>110.pSBA15-K206001-B0034-IspA did not grow<br><br><b>Gel electrophoresis: </b><br>147. pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br>didn’t show on gel<br><br><b>Plasmid Preperation:</b><br>148.1 pSB1C3-gfas purple: 311.6 ng/µl<br>148.2 pSB1C3-gfas purple: 338.0 ng/µl<br>149.1 pSB1C3-meffred: 202.4 ng/µl<br>149.4 pSB1C3-meffred: 310.4 ng/µl<br>150.1 pSB1C3-spispink: 305.4 ng/µl<br>150.2 pSB1C3-spispink: 323.8 ng/µl<br>151.2 pSB1C3-meffblue: 179.2 ng/µl<br><br></div>';
+ }
+ else if(id == 'd2013728')
+ {
+  ds = '<div id="dairy-text"><h1>Sunday 2013-07-28</h1><br><b>Name of participants:</b> Mikael Strandgren (MS), Alona Nyberg(AN), Kristoffer L, Marcus H, Karl H.,Malin, Magnus, Nils, Pontus<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>168. pSBLbC2<br>169. pSBLbEcC2<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br> <br><h2>Todays work</h2><br><b>PCR:</b><br>19. CrtE~(O)-Z/w RBS, ZF<br>20.CrtE~Z/w RBS, ZF<br>21. CrtE~I/wRBS,ZF<br><br><b>Restreak:</b><br>140.pSB3T5-J23110-B0034-UGTCs2-His<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-His.<br><br><b>Spreading of transformed E. coli (from 20130726):</b><br>90.1 BBa_J61002-J23101<br>153. pSB1C3-j23110-meffBlue <br>155. pSB1C3-j23110-meffRed<br>156. pSB1C3-j23106-meffRed<br>157. pSB1C3-j23110-spisPink<br>159. pSB1C3-j23110-gfasPurple<br>160. pSB1C3-j23106-gfasPurple<br>162. pSB1C3-J23101<br>168. pSBLbC2<br>169. pSBLbEcC2<br><br><b>Frozen stock & plasmid preparation:</b><br>90.1 BBa_J61002-J23101<br>90.2 BBa_J61002-J23101<br>153.1 pSB1C3-j23110-meffBlue <br>153.2 pSB1C3-j23110-meffBlue<br>155.1 pSB1C3-j23110-meffRed<br>156.1 pSB1C3-j23106-meffRed<br>157.1 pSB1C3-j23110-spisPink<br>157.2 pSB1C3-j23110-spisPink<br>159.4 pSB1C3-j23110-gfasPurple<br>160.3 pSB1C3-j23106-gfasPurple<br>160.4 pSB1C3-j23106-gfasPurple<br>162.1 pSB1C3-J23101<br>162.2 pSB1C3-J23101<br><br><b>Overnight:</b><br>106.5 pSB3K3-B0034-J23110-TAL<br>106.6 pSB3K3-B0034-J23110-TAL<br>43.7 pSB1C3-B0034-TAL<br>143.1.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>167.1.pSB1C3-fwYellow<br>167.2.pSB1C3-fwYellow <br><br><b>Digest:</b><br>5.pSB4K15-red digested with E,P<br>111.6.pSBLb4C15-red digested with E,P<br>152.1.pUC57(amp)-B0034-ExpTag-Linker-Miraculin-HisTag digested with E,P<br>152.1.pUC57(amp)-B0034-ExpTag-Linker-Miraculin-HisTag digested with X,P<br><br><b>Assembly:</b><br>105. pSB3T5-J23101-B0034-TAL<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br><br><b>Ligation:</b><br>58.pSB1C3-B0034-IspA, 5.pSB4K15-red, 91.BBa_J61002-J23106 --> 108.pSBA15-J23106-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000--> 109.pSBA15-K206000-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001 --> 110.pSBA15-K206001-B0034-IspA<br>3.pSB3T5-red, 125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(E,P), 1.pSB1C3-red->  176.B1C3-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, 103.pSB1K3-J23101 -> 177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, 92.BBa_J61002(amp)_J23110 -> 178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, CP1 -> 179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 3.pSB3T5-red, CP8 -> 180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP1 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP8 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>Transformation:</b><br>105. pSB3T5-J23101-B0034-TAL<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br><br><b>Gel electrophoresis: </b><br>76. pEL3K16-CrtI<br>20. CrtZ<br>19.CrtE~(O)-Z<br>20.CrtE~Z<br>21. CrtE~I<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>147.pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br><br><b>Sequence prep:</b><br>144. pSB1C3-B0034-UGTCs2-His<br><br><h2>Results</h2><br><b>Frozen stock & plasmid preparation:</b><br>90.1 BBa_J61002-J23101: 215.4 ng/µl <br>90.3 BBa_J61002-J23101: 210.1 ng/µl<br>153.1 pSB1C3-j23110-meffBlue: 112.6 ng/µl<br>153.2 pSB1C3-j23110-meffBlue: 119 ng/µl<br>155.1 pSB1C3-j23110-meffRed: 169.5 ng/µl<br>156.1 pSB1C3-j23106-meffRed: 200.3 ng/µl<br>157.1 pSB1C3-j23110-spisPink: 73.7 ng/µl<br>157.2 pSB1C3-j23110-spisPink: 222.1 ng/µl<br>159.4 pSB1C3-j23110-gfasPurple: 279.7 ng/µl<br>160.3 pSB1C3-j23106-gfasPurple: 116.1 ng/µl<br>160.4 pSB1C3-j23106-gfasPurple: 122.8 ng/µl<br>162.1 pSB1C3-J23101: 79.9 ng/µl<br>162.2 pSB1C3-J23101: 80.1 ng/µl<br><br><b>Gel electrophoresis: </b><br>76. pEL3K16-CrtI - Correct at 50 degrese tube<br>20. CrtZ - Correct for low temps<br>19.CrtE~(O)-Z - weak incorrect bands<br>20.CrtE~Z - weak incorrect bands<br>21. CrtE~I - 3 bands, one correct 2 wrong<br>143. pSB3T5-CP8-B0034-UGTCs2-6xHis - 143.1 success, other clones failed<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His - Failed<br>167.1.pSB1C3-fwYellow - Success<br>167.2.pSB1C3-fwYellow - Success<br></div>';
+ }
+ else if(id == 'd2013729')
+ {
+  ds = '<div id="dairy-text"><h1>Monday 2013-07-29</h1><br><b>Name of participants:</b> Malin, Magnus, Pontus, Nils, Christoffer, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alona Nyberg(AN), Nafisa Bashir (NB), Mikael Strandgren (MS), Jens Isaksson (JI)<br><br><h2>Ongoing constructs</h2><br>108.pSB4K15-J23106-B0034-IspA<br>109.pSB4A15-K206000-B0034-IspA<br>110.pSB4A15-K206001-B0034-IspA<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>176.B1C3-B0034-Exp-Linker-Miraculin-His<br>177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>Lb10. plantarum 256-p256<br><br><h2>Todays work</h2><br><b>O/N, Frozen Stock:</b><br>76.pEL3K16-CrtI<br><br><b>Screening & Overnight:</b><br>140.pSB3T5-J23110-B0034-UGTCs2-His<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-His.<br><br><b>Digestion: </b><br>3.     pSB3K3-red<br>36.   pEL3S17-red<br>106. pSB3T5-J23110-B0034-TAL<br><br><b>Assembly:</b><br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br><br><b>Transformation:</b><br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>163.pSB3T5-ZCD-His-B0034-UGTCs2-His<br>176.B1C3-B0034-Exp-Linker-Miraculin-His<br>177.pSB3T5-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pSB3T5-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pSB3T5-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pSB3T5-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>Lb10. plantarum 256 p256 			(as competence test)<br>Lb11. plantarum 256 plasmid free 	(as positive control)<br>Lb12. reuteri DSM-20016 plasmid free 	(as positive control)<br>Lb23. reuteri 100-23-pSBLb4C15-red	(single-ori (pJP059) shuttle vector)<br>Lb24. reuteri DSM-20016-pSBLb4C15-red 		(--”--)<br>Lb25. reuteri 100-23-pSBLbC2-red 	(single-ori (pAMbeta1) shuttle vector)<br>Lb28. reuteri 100-23-pVS2-red 		(as competence test)<br><br><b>Restreak:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br><br><b>Ligation:</b><br>120. pSB1C3-B0034-His-4CL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br><br><b>Gel:</b><br>147.pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br>19. CrtE~(O)-Z/w RBS, ZF<br>20. CrtE~Z/w RBS, ZF + gelextraction<br>21. CrtE~I/w RBS, ZF<br>Lb10. plantarum 256 p256 <br>169. pSBLbEcC2-red (20 clones)*<br><i>-* With the purpose of trying to find clones transformed with plasmids containing the pAMbeta1-ori as well as the E. coli ori.</i><br><br><b>Digest, ligate and transformation:</b> <br>90.1 BBa_J61002-J23101  to 139. pSB3K3-J23101-B0032-BFP<br><br>Two phase separation with octanol and acetic acid. Spectrophotometry of 106.5 and 106.6. Comparison between the Shimadzu UV160, Shimadzu UV1800, and the nanodrop.<br><br><h2>Results</h2><br><b>Gel:</b><br>147.pSB1C3-B0034-Thioredoxin - success<br>165.pSB1C3-tsPurple - success<br>166.pSB1C3-scOrange - success<br>167.pSB1C3-fwYellow - success<br>19. CrtE~(O)-Z/w RBS, ZF - No right band<br>20. CrtE~Z/w RBS, ZF - Right band slightly above 10000bp, thou weak and others,<br>21. CrtE~I/w RBS, ZF - Right band at around 8000bp<br>All tranformations but 176 failed. Ligatoins will be redone, but with pSB3T5 replaced with pEL3S17, as this backbone has shown better performance.<br><br><b>Transformation succes on construct:</b><br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br><br><br></div>';
+ }
+ else if(id == 'd2013730')
+ {
+  ds = '<div id="dairy-text"><h1>Tuesday 2013-07-30</h1><br><b>Name of participants:</b> Christoffer Ahlström (CA), Alona Nyberg (AN), Mikael Strandgren (MS), Malin, Magnus, Pontus, Nils, Christoffer, Sabri, Thorsteinn,  Marcus H, Karl H, Theodor L, Emil M, Victor S<br><br><h2>Ongoing constructs:</h2><br><b>E.coli:</b><br>169. pSBLbEcC2-red<br>191. pSB1A2-fusionGFP<br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>176.pSB1C3-B0034-Exp-Linker-Miraculin-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>104. pSB1C3-B0034-4CL-B0034-STS<br>105. pSB3T5-J23101-B0034-TAL<br>106. pSB3T5-J23110-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3T5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>117. pSB3T5-CP1-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>121.pSB1C3-B0034-STS<br>121.pSB1C3-B0034-His-STS<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>170. pSB3T5-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>173. pSB3T5-CP1-B0034-4CL-B0034-STS<br>174. pSB3T5-CP8-B0034-4CL-B0034-STS<br>175. pSB3T5-J23101-B0034-4CL-B0034-STS<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br><br><h2>Todays work</h2><br><b>Transformation: </b><br>191. pSB1A2-fusionGFP<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>120. pSB1C3-B0034-His-4CL<br>Spectrofotometry (using the nanodrop) of 106.5 and 106.6. Inconclusive results.<br><br><b>Screening:</b><br>140.pSB3T5-J23110-B0034-UGTCs2-His<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-His.<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS clone: 1,2,3,4<br>172. pSB3T5-CP1-B0034-TAL clone: 1,2,3,4<br><br><b>Plasmid preparation:</b><br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>165.pSB1C3-tsPurple<br>166.pSB1C3-scOrange<br>167.pSB1C3-fwYellow<br><br><b>PCR:</b><br>19. CrtE~(O)-Z/w RBS, ZF<br>20. CrtE~Z/w RBS, ZF<br><br><b>Phosphorylation:</b><br>20. CrtE~Z/w RBS, ZF<br>21. CrtE~I/w RBS, ZF<br><br><b>Streaking of strains: </b><br>191. pSB1A2-fusionGFP<br><br><b>Re-streak:  </b><br>139. pSB3K3-J23101-B0032-BFP<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>176.pSB1C3-B0034-Exp-Linker-Miraculin-His<br><br><b>Ligate and transformation: </b><br>90. BBa_J61002-J23101 to 139. pSB3K3-J23101-B0032-BFP<br><br><b>Digest and ligate: </b><br>162. pSB1C3-J23101 to 192.pSB3K3-J23101-B0032-BFP<br>58.pSB1C3-B0034-IspA, 5.pSB4K15-red, 91.BBa_J61002-J23106 --> 108.pSBA15-J23106-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 94.pSB1C3-K206000--> 109.pSBA15-K206000-B0034-IspA<br>58.pSB1C3-B0034-IspA, 11.pSB4A15-red, 95.pSB1C3-K206001 --> 110.pSBA15-K206001-B0034-IspA<br>36. pEL3S17-red, 125.1.pSB1C3-ZCD-His, 127.4.pSB1A3- B0034-UGTCs2-His -> 163. pEL3S17-ZCD-His-B0034-UGTCs2-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 92.BBa_J61002(amp)_J23110 -> 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, CP1 -> 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, CP8 -> 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP1 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 111.pSBLb4C15-red(E,P), CP8 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>O/N, Frozen Stock:</b><br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br><br><b>Gel electrophoresis on:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS clone: 1,2,3,4<br>172. pSB3T5-CP1-B0034-TAL clone: 1,2,3,4<br><br><h2>Results</h2><br><b>Previous day: </b><br><br><b>Transformation:</b><br>Lb10. plantarum 256 p256	 			growing, possibly contaminated<br>Lb11. plantarum 256 plasmid free 		growing, possibly contaminated<br>Lb12. reuteri DSM-20016 plasmid free 		growing, possibly contaminated<br>Lb23. reuteri 100-23-pSBLb4C15-red:		no growth<br>Lb24. reuteri DSM-20016-pSBLb4C15-red 	no growth<br>Lb25. reuteri 100-23-pSBLbC2-red 		no growth<br>Lb28. reuteri 100-23-pVS2-red 			no growth<br><i>→ Do new competent cells and transformation immediately</i><br><br><b>Today:</b><br><b>Screening-pcr/Gel electrophoresis: </b><br>169. pSBLbEcC2-red (20 clones): No correct bands, just smudges.<br><i>→ Screen 20 new colonies for correct plasmid, use phusion polymerase and pEL-primers, giving some kind of (bigger) fragments regardless of whether the clone has the correct plasmid or not. </i><br></div>';
+ }
+ else if(id == 'd2013731')
+ {
+  ds = '<div id="dairy-text"><h1>Wednesday 2013-07-31</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Thorsteinn, Nils, Malin, Alona Nyberg (AN), Christoffer Ahlström (CA), Anton Berglund (AB), Mikael Strandgren (MS), Jens Isaksson (JI), Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E<br><br><h2>Ongoing constructs</h2><br><b>E.coli:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>169. pSBLbEcC2-red<br>191. pSB1A2-fusionGFP<br>139. pSB3K3-J23101-B0032-BFP<br>192.pSB3K3-J23101-B0032-BFP<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br><b>L. reuteri:</b><br>4.1 100-23 no plasmid<br>12.1 DSM 20016 no plasmid<br><br><h2>Todays work</h2><br><b>Screening:</b><br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>176.pSB1C3-B0034-Exp-Linker-Miraculin-His<br>169. pSBLbEcC2-red (20 clones)<br> <br><b>Overnight:</b><br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>176.pSB1C3-B0034-Exp-Linker-Miraculin-His<br>Lb4.1 reuteri 100-23 no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br>172. pSB3T5-CP1-B0034-TAL (clone 3)<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS (clone 3 and 4)<br>189. pSB3K3-J23101-B0034-TAL (clone 2 and 3)<br><br><b>Ligation:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 92.BBa_J61002 (amp)-J23110 -><br>140.pEL3S17-Red-J23110-B0034-UGTCs2-6xHis <br>36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis<br><br><b>Transformation:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pEL3S17-Red-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis<br>143.pSB3T5-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>139. pSB3K3-J23101-B0032-BFP<br>192.pSB3K3-J23101<br><br><b>Re-Streak:</b><br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>191.1 pSB1A2-fusionGFP<br>191.2 pSB1A2-fusionGFP<br>191.3 pSB1A2-fusionGFP<br>191.4 pSB1A2-fusionGFP<br>139. pSB3K3-J23101-B0032-BFP<br>192.pSB3K3-J23101-B0032-BFP<br><br><b>Gel:</b><br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1  [Linear from PCR mutagenesis of 19] <br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 [linear from PCR mutagenesis of 20]<br><br><b>Preparation for sequencing: </b><br>153. pSB1C3-j23110-meffBlue <br>155. pSB1C3-j23110-meffRed<br>156. pSB1C3-j23106-meffRed<br>157. pSB1C3-j23110-spisPink<br>159. pSB1C3-j23110-gfasPurple<br>160. pSB1C3-j23106-gfasPurple<br>150. pSB1C3-spispink<br>151. pSB1C3-meffblue<br>149. pSB1C3-meffred<br>148. pSB1C3-gfas purple<br><br><b>Assembly:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br><b>PCR cloning and screening on gel of constructs:</b><br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br><br><h2>Results</h2><br><b>Screening:</b><br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis Failed<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis Failed<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis One colony succeded<br>Tranformations of 108, 109, 110, 177, 141, 180, and 182 failed.<br>Tranformations of 163, 178 and 179 succeeded.<br>Transformations of 181 and 140 remain inconclusive.<br><br><b>Screening of PCR products:</b><br>188.3 , 188.4, 189.2 and 189.3 have correct bands on the gel<br><br><h2>Other experiments</h2><br>LB-agar plates making: <br>Ampicillin, 28 plates<br>Chloramphenicol, 30 plates<br>Kanamycin, 31 plates<br>Spectinomycin, 26 plates<br>Tetracycline, 29 plates<br></div>';
+ }
+else if(id == 'd201381')
+ {
+  ds = '<div id="dairy-text"><h1>Thursday 2013-08-01</h1><p>Name of participants: Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E<br><br><h2>Ongoing constructs:</h2>' +
+  '114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>'+
+  '137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>'+
+  '187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br>'+
+  '<h2>Todays work</h2><b>PCR screening and gel electrophorersis of construct:</b><br>185.(5-10) pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>'+
+  '<h2>sequencing prep. of constructs:</h2><br>185.9-185.10<br>'+
+  '<h2>Transformation of constructs:</h2><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br>'+
+  '<h2>Results</h2>Construct 185, clone 9 and 10, bonds around 3000/4000 bp.<br><br>'+
+  '<h2>Follow up</h2><br>- <br><br>'+
+  '<h2>Other experiments</h2><br>-<br></p></div>';
+ }
+   else if(id == 'd201382')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-02</h1><br><b>Name of participants: </b>Anton B, Christoffer A, Mikael S, Jens I, Marcus H, Karl H, Lovisa P, Emil M, Victor S, Hampus.E, Magnus, Pontus, Sabri, Nils<br><br><h2>Ongoing constructs:</h2><br><b>E.coli:</b><br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>108. pSBA15-J23106-B0034-IspA<br>109. pSBA15-K206000-B0034-IspA<br>110. pSBA15-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3T5-J23110-B0034-His-TAL<br>119. pSB1C3-B0034-4CL<br>120. pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>140. pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141. pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142. pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163. pEL3S17-ZCD-His-B0034-UGTCs2-His<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>177. pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178. pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183. pEL3A15-B0034-CrtZ<br>184. pSB1C3-B0034-CrtZ<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>191. pSB1A2-fusionGFP<br>193. pSB1C3-amilGFP<br>194. pSB1C3-B0034-amilCP<br>195. pSB1C3-cjBlue<br>196. pSB1C3-eforRed<br>197. pSB1C3-aeBlue<br>198. pSB1C3-B0034-amilGFP<br>199. pSb1C3-amajCFP<br>200. pSB1C3-B0034-asCP<br>202. pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>204. pEL3S17-J23110-HIS-STS<br>205. pSBLB4C15-CP1-B0034-HIS-TAL<br>206. pSB1C3-J23101-B0034-TAL<br>207. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><b>L. reuteri:</b><br>3. DSM 20016 pVS2<br>4. 100-23 no plasmid<br>12. DSM 20016 no plasmid<br>23. 100-23 pSBLb4C15-red<br>24. DSM 20016 pSBLb4C15-red<br>26. 100-23 pSBLbEcC2-red<br>27. DSM 20016 pSBLbEcC2-red<br>28. 100-23 pVS2<br><br><h2>Todays work</h2><br><b>Plasmid preparation: </b><br>139.1.1 pSB3K3-J23101-B0032-BFP <br>139.1.2 pSB3K3-J23101-B0032-BFP<br>191.1 pSB1A2-fusionGFP<br>191.2 pSB1A2-fusionGFP<br><br><b>Ligation:</b><br>4.pSB1A3-Red, 147.pSB1C3-B0034-Thioredoxin, 125.pSB1C3-ZCD-His-><br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>36.pEL3S17-Red, 127. pSB1A3-B0034-UGTCs2-His, 90.BBa_J61002 (amp)-J23101 -> 141.pEL3S17-Red-J23101-B0034-UGTCs2-6xHis<br>152.pUC57-B0034-Exp-Linker-Miraculin-His(X,P), 36.pEL3S17-red, 103.pSB1K3-J23101 -> 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br><br><b>Streaking of plasmid-free cells from frozen stock as control of C-resistance: </b><br>Lb4.1 reuteri. 100-23 no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak of construct:</b><br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23101-B0034-TAL<br><br><b>Screening:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Overnight:</b><br>99. pCB1C3 - CrtO<br>190. pEL3K16 - CrtO<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Gel electrophoresis:</b><br>PCR of 152. pUC57-B0034-Exp-Linker-Miraculin-Linker-His to extract Miraculin with biobrick prefix and suffix<br><br><h2>Results</h2><br><b>Today:</b><br><b>Plasmid preparation:</b> <br>139.1.1 pSB3K3-J23101-B0032-BFP: 51.9 ng/µl <br>139.1.2 pSB3K3-J23101-B0032-BFP: 53.6 ng/µl<br>191.1 pSB1A2-fusionGFP: 78.9 ng/µl<br>191.2 pSB1A2-fusionGFP: 74.1 ng/µl</div>'
+ }
+    else if(id == 'd201383')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>'
+ }
+    else if(id == 'd201383')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-03</h1><br><b>Name of participants: </b>Lovisa P, Magnus, Pontus<br><br><h2>Other experiments</h2><br><b>Making competent cells (D5α)</b></div>'
+ }
+     else if(id == 'd201384')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-04</h1><br><b>Name of participants: </b>Emil M, Magnus, Pontus<br><br><h2>Ongoing constructs:</h2><br>108.pSBA15-J23106-B0034-IspA<br>109.pSBA15-K206000-B0034-IspA<br>110.pSBA15-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pSB3T5-J23101-B0034-UGTCs2-6xHis<br>142.pSB3T5-CP1-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br><br><h2>Todays work:</h2><br><b>Overnight:</b><br>10. D5α<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 1)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2Other experiments:</b><br><b>Competent cell test</b><br><br></div>'
+ }
+     else if(id == 'd201385')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-05</h1><br><b>Name of participants: </b>Anton B, Viktor T, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2<br><br><b>E. coli:</b><br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Todays work</h2><br><b>Competent cells</b><br>Lb4.1 reuteri 100-23 no plasmid<br><br><b>Lambda red transformation:</b><br>Crt<br><br><b>Transformation:</b><br>reuteri 100-23 pSB1C3-red (1 tube, to see if it would work in Lb, died in electroporation)<br>Lb23. reuteri 100-23 pSBLb4C15-red (5 tubes, 1 died in electroporation)<br>Lb25. reuteri 100-23 pSBLbC2-red (1 tube)<br>Lb28. reuteri 100-23 pVS2 (2 tubes, 1 died in electroporation)<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><h2>Characterization</h2><br><b>Detection of TAL activity through spectroscopy (day 2)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other experiments</h2><br><b>Casting LB-agar plates: </b><br>Kanamycin, 63 plates<br>Chloramphenicol, 61 plates<br>Spectinomycin, 65 plates<br>Tetracycline, 32 plates<br>Ampicillin, 30 plates<br><br><b>Competent cells (D5α)</b></div>'
+ }
+      else if(id == 'd201386')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-06</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Thorsteinn, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Anton B, Mikael S, Viktor T<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>57.3.pSB1C3-B0034-Idi<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>6. pSB1K3-red<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>111. pSBLb4C15-red<br>139. pSB3K3-J23101-B0032-BFP<br>165. pSB1C3-RBS-tsPurle<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>194. pSB1C3-B0034-amilCP<br>198. pSB1C3-B0034-amilGFP<br><br><b>L. reuteri:</b><br>23. 100-23 pSBLb4C15-red<br>25. 100-23 pSBLbC2-red<br>28. 100-23 pVS2 <br><br><h2>Todays work:</h2><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Restreak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>115. pSB3K5-J23101-B0034-His-TAL<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Digest:</b><br>6. pSB1K3-red<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>68.2 pSB3K3-CP1-B0032-BFP (EcoRI + PstI)<br>69.4 pSB3K3-CP8-B0032-BFP (EcoRI + PstI)<br>70.3 pSB3K3-CP11-B0032-BFP (EcoRI + PstI)<br>71.1 pSB3K3-CP29-B0032-BFP (EcoRI + PstI)<br>72.2 pSB3K3-CP30-B0032-BFP (EcoRI + PstI)<br>73.4 pSB3K3-CP41-B0032-BFP (EcoRI + PstI)<br>74. pSB3K3-CP44-B0032-BFP (EcoRI + PstI)<br>111. pSBLb4C15-red (XbaI + PstI)<br>165. pSB1C3-RBS-tsPurle (XbaI + PstI)<br>166. pSB1C3-RBS-scOrange (XbaI + PstI)<br>167. pSB1C3-RBS-fwYellow (XbaI + PstI)<br>194. pSB1C3-B0034-amilCP (XbaI + PstI)<br>198. pSB1C3-B0034-amilGFP (XbaI + PstI)<br><br><b>Ligation:</b><br>B0034-Thioredoxin, 1. pSB1C3-red (E, P) -> 147. pSB1C3-B0034-Thioredoxin<br>B0034-Thioredoxin, 1. pSB1C3-red (X, P) -> 147. pSB1C3-B0034-Thioredoxin<br><br><b>Gel: </b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br><br><b>Screening:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br>204.(1-4) pEL3S17-J23110-B0034-HIS-STS<br>171(1-4) pEL3S17-J23110-B0034-4CL-B0034-STS<br><br><b>Mutagenesis:</b><br>43.7  pSB1C3-B0034-TAL<br>57.3.pSB1C3-B0034-Idi -> 129.pSB1C3-Idi(mut)<br><br><v>Sequencing preparation:</b><br>139.1 pSB3K3-J23101-B0032-BFP<br>185.9 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>185.10 pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>O/N:</b><br>204.2 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br>111.7 pSBLb4C15-red<br><br><h2>Results:</h2><br><b>Gel:</b> <br>164.E.Coli Nissle-CrtY (clones from transformation plate) failed<br>201.E.Coli Nissle-CrtY-CrtI failed<br><br><b>Previous day:</b><br><b>Transformation: </b><br>No visual growth. Keeping them on growth for one more day.<br><br><h2>Characterization:</h2><br><b>Detection of TAL activity through spectroscopy (day 3)</b><br><b>Contructs tested for TAL activity:</b><br>106.5 pSB3K3-J23110-B0034-TAL <br>106.6 pSB3K3-J23110-B0034-TAL<br>137.1 pSB3K3-CP8-B0034-TAL<br>189.3 pSB3K3-J23101-B0034-TAL<br><br><b>Negative controls:</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><b>Positive controls (p-coumaric acid added before dextraction):</b><br>43.7 pSB1C3-B0034-TAL<br>119.2 pSB1C3-B0034-4CL<br><br><h2>Other Experiments:</h2><br>10.D5Alpha competence test<br><br><b>Preparation of buffers for Western Blot</b><br><br><b>Casting LB-agar plates:</b><br>Kanamycin, 33 plates<br>Spectinomycin, 31 plates<br><br></div>'
+ }
+      else if(id == 'd201387')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-07</h1><br><b>Name of participants: </b>- Viktor T, Anton B, Mikael S, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>6. pSB1K3-red<br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>92. J23110<br>111. pSBLb4C15-red<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple<br>241. pSB1K3-J23110-RBS-scOrange<br>242. pSB1K3-J23110-RBS-fwYellow<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><h2>Todays work</h2><br><b>Assembly:</b><br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple*<br>241. pSB1K3-J23110-RBS-scOrange*<br>242. pSB1K3-J23110-RBS-fwYellow*<br><i>*Will eventually be tried in shuttle vector, but that has C-resistance, so a subcloning to K is necessary first, or a modification to change the resistance of the shuttle vector.</i><br><br><b>Transformation:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>240. pSB1K3-J23110-RBS-tsPurple<br>241. pSB1K3-J23110-RBS-scOrange<br>242. pSB1K3-J23110-RBS-fwYellow<br>108.pEL3S17-J23106-B0034-IspA<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br><br><b>Spreading: </b><br>60. PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br><br><b>Restreak:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>O/N & Screening:</b><br>110.pEL3S17-K206001-B0034-IspA<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pBAD/AraC-RBS-Luciferas (2013 years kit)<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><b>Gel:</b><br>164.E.Coli Nissle-CrtY (clones from transformation plate)<br>201.E.Coli Nissle-CrtY-CrtI<br>129.pSB1C3-Idi(mut)<br><br><h2>Results</h2><br><b>Transformation (Monday):</b><br>Still no visual growth. <br><br><b>SDS-page failed (probably due to faulty buffers, investigate the running buffer first)</b><br><br><h2>Characterization experiments</h2><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br><br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><h2>Other experiments</h2><br><b>Sequencing preparation:</b><br>69. pSB3K3-CP8-B0032-BFP*<br><i>*since the existing sequencing data doesn’t cover the whole insert with sufficient accuracy. There may be a mutation at the end of BFP, but that is more than 800bp after the single primer. Was not sent today, not sequencing tuesday.</i></div>'
+ }
+      else if(id == 'd201388')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-08</h1><br><b>Name of participants:</b> Magnus, Pontus, Sabri, Malin, Niclas, Alexander, Nils, Marcus H, Lovisa P, Emil M, Victor S, Hampus.E, Kristoffer L, Malin B, Stephanie H, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>60. Strain PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br>139. pSB3K3-J23101.B0032-BFP<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSb1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br><br><h2>Todays work:</h2><br><b>Transformation:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>no nr. pSB1c3-Thioredoxin<br>no nr. pEL3S17-Thioredoxin<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><b>Re-streak:</b><br>108.pEL3S17-J23106-B0034-IspA<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><bPlasmid prepp</b><br>226. pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pBAD/AraC-RBS-Luciferas (2013 years kit)<br>245. pSB1C3-J23110-B0034-amajCP(amajLime)<br>246. pSB1C3-J23110-b0034-gfasPurple<br><br><b>O/N screening:</b><br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Two step Assembly</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br><br><b>Overnight (screening)</b><br>1. pSB1C3-red<br><br><b>O/N</b><br>60. Strain PK401 dam- no plasmid<br>111.7 pSBLb4C15-red<br><br><b>Streak from frozen stock</b><br>3. pSB3T5-red<br>6. pSB1K3-red<br>42. pSB4S15-red<br>77. pSB4C15-red<br><br><h2>Results:</h2><br><b>One of the SDS-gels failed, resulting in no good gel to stain (faulty buffers, continue to search for sources of errors)</b><br><br><b>The succesfull gel was prepared with control buffers.</b><br><br><b>Transformation of promotors with BFP in the shuttle vector, for characterization in Lactobacillus: Some growth, only white colonies, will let them grow overnight again. </b><br><br><h2>Characterization experiments:</h2><br><b>Westernblot </b><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>Electro transfer over night</b><br><br><b>Staining of control gel</b><br><br><b>Detection of TAL activity through spectroscopy (day 4)</b></div>'
+ }
+      else if(id == 'd201389')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-09</h1><br><b>Name of participants:</b> Stephanie H, Mikael S, Anton B, Anders E, Magnus, Pontus, Sabri, Malin, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br>60. PK401 dam- no plasmid<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br>111.7 pSBLb4C15-red<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110 <br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>60. PK401 dam- no plasmid<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>O/N:</b><br>60.3 PK401 dam- no plasmid*<br><i>*The O/N made on thursday used MRS, so it had to be remade from restreaks.</i><br><br><b>Plasmid preparation:</b><br>111.7 pSBLb4C15-red<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Restreak:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin (E,P & E,X)<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>no nr. pEL3S17-Thioredoxin<br><br><b>Amplification PCR</b><br>134. B0034-CBP<br>135. B0034-CSP<br>254. Gus<br>257. p-pem<br>258. pem<br><br><b>Mutagenesis</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>221. pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>Send to Linköping, as plasmid prepp and agar stabb</b><br>226.pSB1C3-pBAD/AraC-RBS-Luciferas (2012 years kit)<br>227.pSB1C3-pBAD/AraC-RBS-Luciferas (2013 years kit)<br><br><b>Transformation</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>111.7 pSBLb4C15-red: 30,6 ng/µl<br><br><br><b>O/N:</b><br>221 failed, redo.<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA - Screening gel fail<br>109.pEL3S17-K206000-B0034-IspA - Screening gel fail<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis - <br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis - <br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His - Screening gel fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His - <br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 - Screening gel fail<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - Screening gel fail<br>219.pSB1C3-Miraculin - <br>221. pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI - Screening gel fail</div>'
+ }
+      else if(id == 'd2013810')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-10</h1><br><b>Name of participants: </b>Anders Edlund, Marcus H, Hampus.E, Kristoffer L, Ken B-A, Nils, Alexander, Sabri, Pontus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>134. B0034-CBP<br>135. B0034-CSP<br>254. Gus<br>257. p-Toxin-Antitoxin<br>258. Toxin-Antitoxin<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>114. pSB1C3-B0034-TAL(MUT)<br>105. pSB3T5-J23101-B0034-TAL<br>107. pSB3T5-CP1-B0034-TAL<br>115. pSB3K5-J23101-B0034-His-TAL<br>119.pSB1C3-B0034-4CL<br>120.pSB1C3-B0034-HIS-4CL<br>137. pSB3K3-CP8-B0034-TAL<br>138. pSB3K3-CP8-B0034-His-TAL<br>139. pSB3K3-J23101-B0034-BFP<br>171. pSB3T5-J23110-B0034-4CL-B0034-STS<br>172. pSB3T5-CP1-B0034-TAL<br>185. pEL3S17-J23110-B0034-TAL-B0034-4CL-B0034-STS<br>186. pEL3S17-CP1-B0034-4CL-B0034-STS<br>187. pEL3S17-CP8-B0034-4CL-B0034-STS<br>188. pEL3S17-J23101-B0034-4CL-B0034-STS<br>189. pSB3K3-J23101-B0034-TAL<br>204. pEL3S17-J23110-HIS-STS<br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>224. pSB1C3-J23101-B0032-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut)<br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>E. coli PK401 dam-:</b><br>60. no plasmid<br><br><h2>Todays work:</h2><br><b>Gel-electrophoresis</b><br>134. B0034-CBP*<br>135. B0034-CSP*<br>254. Gus<br>257. p-Toxin-Antitoxin<br>258. Toxin-Antitoxin<br>*PCR 99% failed<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>115. pSB3K5-J23101-B0034-His-TAL<br>114. pSB1C3-B0034-TAL(MUT)<br>120.pSB1C3-B0034-HIS-4CL<br><br><b>Amplification PCR</b><br>134. B0034-CBP<br>135. B0034-CSP<br><br><b>ON:</b><br>60. E. coli dam-<br><br><b>Assembly and subcloning:</b><br>205.pSBLB4C15-CP1-B0034-HIS-TAL<br>206.pSB1C3-J23110-B0034-TAL<br>225. pEL3S17-J23101-B0032-TAL-B0034-4cl-B0034-STS<br>259. pEL3S17-B0034-4CL-B0034-STS<br>127 + 103 + 36 -> 253. pEL3S17-J23101-B0034-UGTCs2-His<br>152 + 61 + 111 -> 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>152 + 62 + 111 -> 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>95 + 58 + 36 -> 110.pEL3S17-K206001-B0034-IspA<br>CrtZ + 1 -> 184.pSB1C3-B0034-CrtZ<br>Miraculin + 1 -> 219.pSB1C3-Miraculin<br><br><b>O/N Screening:</b><br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Link<br>er-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>219.pSB1C3-Miraculin<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>201. E.Coli. Nissle - CrtY-CrtI<br><br><b>DPN1</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>115 good, to sequencing<br>114 good, to sequencing<br>120 pick new clones and screen<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 - Succes<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 - fail<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 -fail<br><br><b>Characterization experiments</b><br><b>Detection of TAL activity through spectroscopy (day 5)</b></div>'
+ }
+      else if(id == 'd2013811')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-11</h1><br><b>Name of participants: </b>Anders E, Mikael S, Nafisa B, Nils, Alexander, Sabri, Malin<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>60. PK401 dam- no plasmid<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>91. BBa_J61002-J23106<br>92. BBa_J61002-J23110 <br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>165. pSB1C3-RBS-tsPurple<br>166. pSB1C3-RBS-scOrange<br>167. pSB1C3-RBS-fwYellow<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work:</h2><br><b>Competent cells:</b><br>60. PK401 dam- noplasmid<br><br><b>Transformation:</b><br>275. PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15 cut with E and P<br>and more.<br><br><b>Digest:</b><br>111.7 pSBLb4C15 cut with E and P<br>111.7 pSBLb4C15 cut with SacI and SalI<br>254. Gus P+X   <br>257. p-Toxin-Antitoxin P+X <br>258. Toxin-Antitoxin P+X<br>134. B0034-CBP E+S<br>1.  pSB1C3-red E+S<br>3. pSB3T5-red E+P<br><br><b>PCR purification:</b><br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>134. pSB1C3-B0034-CBP<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><b>Plasmidprepp:</b><br>108.2.pEL3S17-J23106-B0034-IspA<br>108.3.pEL3S17-J23106-B0034-IspA<br>148. pSB1C3-RBS-gfasPurple<br>150. pSB1C3-RBS-spisPink<br>151. pSB1C3-RBS-meffBlue<br>163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>243. pSB1A2-LacI<br>244. pSB1C3-Promoter-RBS-Bleomysin<br>228.pEL3A17-Blue<br>229.pEL3K17-Blue<br>230.pEL3S15-Blue<br>231.pEL3C17-Blue<br><br><b>Two part assembly:</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br><br><b>O/N:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br><br><b>Sekvensprepp:</b><br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Re-circularization:</b><br>202.pSB4C5.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 <br><br><b>Re-streak</b><br>165.1 pSB1C3-RBS-tsPurple, 91. BBa_J61002-J23106 à 247. pSB1C3-J23110-RBS-tsPurple<br>165.1 pSB1C3-RBS-tsPurple, 92. BBa_J61002-J23110 à 248. pSB1C3-J23106-RBS-tsPurple<br>166.1 pSB1C3-RBS-scOrange, 91. BBa_J61002-J23106 à 249. pSB1C3-J23110-RBS-scOrange<br>166.1 pSB1C3-RBS-scOrange, 92. BBa_J61002-J23110 à 250. pSB1C3-J23106-RBS-scOrange<br>167.1 pSB1C3-RBS-fwYellow, 91. BBa_J61002-J23106 à 251. pSB1C3-J23110-RBS-fwYellow<br>167.1 pSB1C3-RBS-fwYellow, 92. BBa_J61002-J23110 à 252. pSB1C3-J23106-RBS-fwYellow<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15 cut with E and P: correct bands!<br>and more.</div>'
+ }
+      else if(id == 'd2013812')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1><br><b>Name of participants: </b>Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>149. pSB1C3-B0034-Thioredoxin<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>114. pSB1C3-B0034-TAL-mut<br>115. pSB3K3-J23101-B0034-His-TAL<br>204. pEL3S17-J23110-B0034-HIS-STS<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work</h2><br><b>Ligation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Digest:</b><br>4.pSB1A3-red<br>6.pSB1K3<br>125. pSB1C3-ZCD-His<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>275 PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>O/N Screening:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><b>Transformation</b><br>134. pSB1C3-CBP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Spreading:</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>Plasmidprepp:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Sequence prepp:</b><br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Transformation:</b><br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><h2>Results:</h2><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His failed<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His failed<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Mixing new buffers for western blot</b><br><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 55</div>'
+ }
+      else if(id == 'd2013812')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-12</h1><br><b>Name of participants: </b>Alexander, Sabri, Malin, Pontus, Niclas, Sabri, Hampus.E, Theodor L, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Lovisa P, Anders E, Mikael S, Nafisa B, Viktor B, Viktor T, Christoffer A, Stephanie H, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>149. pSB1C3-B0034-Thioredoxin<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>114. pSB1C3-B0034-TAL-mut<br>115. pSB3K3-J23101-B0034-His-TAL<br>204. pEL3S17-J23110-B0034-HIS-STS<br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work</h2><br><b>Ligation:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>273.pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-Gus<br>257. pSB1C3-p-Toxin-Antitoxin (with promotor)<br>258. pSB1C3-Toxin-Antitoxin<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Digest:</b><br>4.pSB1A3-red<br>6.pSB1K3<br>125. pSB1C3-ZCD-His<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>275 PK401 dam- pSBLb4C15-red (dam-)<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><br><b>O/N Screening:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>225. pEL3S17 -J23101-B0034-TAL-B0034-4CL-B0034-STS<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><b>Transformation</b><br>134. pSB1C3-CBP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>233. pSBLb4C15-CP1-B0034-BFP<br>234. pSBLb4C15-CP8-B0034-BFP<br>235. pSBLb4C15-CP11-B0034-BFP<br>236. pSBLb4C15-CP29-B0034-BFP<br>237. pSBLb4C15-CP30-B0034-BFP<br>238. pSBLb4C15-CP41-B0034-BFP<br>239. pSBLb4C15-CP44-B0034-BFP<br><br><b>Spreading:</b><br>68. pSB3K3-CP1-B0032-BFP<br>69. pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-CP44-B0032-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br><br><b>Plasmidprepp:</b><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Sequence prepp:</b><br>129. pSB1C3-B0034-Idi (mut)<br>149. pSB1C3-B0034-Thioredoxin<br><br><b>Transformation:</b><br>202. pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>PCR:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br>CBP<br>Mcherry<br>Pem<br>Pemp<br><br><h2>Results:</h2><br>222.1.pSB3K3-CP1-B0034-UGTCs2-His failed<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His failed<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis failed<br><br><b>Gel electrophoresis:</b><br>Lb2. reuteri pLUL631 (erythromycin resistance cassette, with primers pLUL-E-sc_F and pLUL-E-sl_R)<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Mixing new buffers for western blot</b><br><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 55</div>'
+ }
+      else if(id == 'd2013813')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-13</h1><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Anton Berglund, Viktor Törnblom, Viktor Blomkvist, Christoffer Ahlström, Stephanie Herman, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L. Lovisa P, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>114.(2-3) pSB1C3-B0034-TAL-mut<br>115.(1-3) pSB3K3-J23101-B0034-His-TAL<br>204.(1-3) pEL3S17-J23110-B0034-HIS-STS<br><br><h2>Todays work:</h2><br><b>O/N Screening glycerolstock:</b><br>110.pEL3S17-K206001-B0034-IspA<br>219.pSB1C3-Miraculin<br>253.pEL3S17-J23101-B0034-UGTCs2-His<br><br><b>Assembly</b><br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-meffRed à 155. pSB1C3-j23110-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-meffRed à 156. pSB1C3-j23106-RBS-meffRed<br>pSB1C3-Blue, pSB1C3-J23110, pSB1C3-spisPink à 157. pSB1C3-j23110-RBS-spisPink<br>pSB1C3-Blue, pSB1C3-J23106, pSB1C3-spisPink à 158. pSB1C3-j23106-RBS-spisPink<br><br><b>Ligation:</b><br>134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product])<br>134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product])<br>255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])<br>255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product])<br>256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product])<br>256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product])<br><br><b>Plasmidprepp:</b><br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Sequencing prep:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>114.(2-3) pSB1C3-B0034-TAL-mut<br>115.(1-3) pSB3K3-J23101-B0034-His-TAL<br>204.(1-3) pEL3S17-J23110-B0034-HIS-STS<br>108.2.pEL3S17-J23106-B0034-IspA<br>108.3.pEL3S17-J23106-B0034-IspA<br>163.1.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>142.3.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>222.1.pSB3K3-CP1-B0034-UGTCs2-His<br>222.3.pSB3K3-CP1-B0034-UGTCs2-His<br><br><b>Re-streaking:</b><br>134. pSB1C3-CBP (CBP1 [134.1] from digested plasmids [PCR-product])<br>134. pSB1C3-CBP (CBP2 [134.2] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus1 [254.1] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus2 [254.2] from digested plasmids [PCR-product])<br>254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product])<br>255. pSBLb4C15-pemp (pemp1 [257.1] from digested plasmids [PCR-product])<br>255. pSBLb4C15-pemp (pemp2 [257.2] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem1 [258.1] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem2 [258.2] from digested plasmids [PCR-product])<br>256. pSBLb4C15-pem (pem3 [258.3] from digested plasmids [PCR-product])<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><b>Re streak 14/8 or 15/8</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br><br><b>Transformation & plating</b><br>134.1 pSB1C3-CBP (134.1 from digested plasmids [PCR product])<br>134.2 pSB1C3-CBP (134.2 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.1 from digested plasmids [PCR product])<br>254.1 pSB1C3-gus (254.2 from digested plasmids [PCR product])<br>255.2 pSBLb4C15-pemp (257.2 from digest plasmids [PCR product])<br>255.1 pSBLb4C15-pemp (257.1 from digest plasmids [PCR product])256.1 pSBLb4C15-pem (258.1 from digested plasmids [PCR product])<br>256.2 pSBLb4C15-pem (258.2 from digested plasmids [PCR product])<br>225. pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>261. pSB3K3-J23110-B0034-HIS-4CL<br>120. pSB3K3-B0034-HIS-4CL<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>177pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br><br><b>Re-streaking</b><br>254. pSB1C3-gus (gus3 [254.3] from digested plasmids [PCR-product]): 1 clone<br>255. pSBLb4C15-pemp (pemp3 [257.3] from digested plasmids [PCR-product]): 4 clones<br><br><b>O/N:</b><br>275. pSBLb4C15-red (dam-)<br>276. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam)<br>277. pSBLb4C15-CP1-Exp-B0034-Linker-Miraculin-His (dam)<br><br><b>Gradient PCR:</b> <br>CBP (colony PCR from Lb.Reuteri)<br>CSP (colony PCR from Lb.Reuteri)<br>Mcherry (from plasmid preparation)<br>256. pSBLb4C15-pem<br><br><h2>Results</h2><br>One of the two gels from SDS page was succesfull, still have problems with the buffers<br><br><h2>Characterization</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><br><b>Western blot</b><br><b>SDS-page on constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><br><b>Electro transfer over night</b><br><br><h2>Other experiments</h2><br><b>Casting LB-agar plates:</b><br>Chloramphenicol: 85<br></div>'
+ }
+      else if(id == 'd2013814')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-14</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Mikael S, Viktor B, Viktor T, Alexander, Malin, Pontus, Niclas, Christoffer, Nils, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H, Theodor L., Peter C, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><b>E. coli PK401 dam-:</b><br>111.7 pSBLb4C15-red<br>181. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Todays work:</h2><br><b>Re-streak:</b><br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>pSB1K3-J23110-B0034-aeBlue (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-AmilCP (should be subcloned to pSB1C3 after)<br>pSB3K3-J23110-B0034-asCP (should be subcloned to pSB1C3 after)<br>Re streak 15/8 or 16/8<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>O/N:</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>Streak from frozen stock:</b><br>111. pSBLb4C15-red<br><br><b>Gradient touchdown colony PCR:</b><br>CSP <br><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CSP (colony PCR from Lb.Reuteri)<br>Mcherry (from plasmid preparation)<br>256. pSBLb4C15-pem<br><br><b>PCR purification: </b><br>M1 <br>M2<br>M3<br>M4<br>CB1<br>CB2<br>256. pSBLb4C15-pem (P1)<br><br><b>Digestion:</b><br>M3 (x+p)<br>CB1 (x+p)<br>P1 (x+p)<br>111.7 pSB4C15-red (e+p)<br>Bleomycin resistance cassette (SacI)<br>pEL PCR product with e. coli ori (ClaI)<br>Lb3 reuteri DSM 20016 pVS2 (ClaI)<br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Ligation:</b><br>M3 with pSB1C3, pSBLB4C15 + CP8, CP1<br>CB1 with pSB1C3<br>P1 with pSB1C3<br>pVS2 origin of replication with pEL PCR-product, cut by ClaI <br>CP promoters with amilGFP, aeBlue,tsPurple<br><br><b>Frozen stock and plasmid preparation:</b><br>275.1 pSBLb4C15-red (dam-)*<br>275.2 pSBLb4C15-red (dam-)<br>276. pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-)<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-)<br><i>*O/N of clone 1 was made from a very small colony, its colour wasn’t as strong as clone 2’s after growing overnight.</i><br><br><b>Transformation:</b><br>109.pEL3S17-K206000-B0034-IspA x<br>110.pEL3S17-K206001-B0034-IspA x<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His x<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His x<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His x<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His x<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His x<br>184.pSB1C3-B0034-CrtZ x<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His x<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His x<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His x<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His x<br>267.psB3K3-K206000-B0034-IspA x<br>268.psB3K3-K206001-B0034-IspA x<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His x<br>272 pSB1K3-B0034-Thioredoxin-ZCD-His x<br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His x<br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His x<br><br><b>Plasmid prepp:</b><br>280. pSB1A3-B0034-aeBlue (should be subcloned to pSB1C3 after, give Erik the subclones version)<br><br><h2>Results:</h2><br><b>Gel Electrophoresis:</b><br>CBP (colony PCR from Lb.Reuteri)<br>CB1: 54.3o C (NC ok!), CB2: 63.3o C (NC ok!) → Correct bands!!<br>CSP (colony PCR from Lb.Reuteri) <br>CS1: 55.7o C (NC ok!), CS2: 65.6o C (NC ok!) → No bands!! (not even primer dimer) <br>Mcherry (from plasmid preparation)<br>M1: 45.0o C (NC ok!), M2: 47.9o C (NC ok!), M3: 50.5o C (NC ok!), M4: 54.3o C (NC ok!) → Correct bands!!<br>256. pSBLb4C15-pem: <br>P1: 47.9o C (NC ok!) → Correct bands!!<br><br><b>PCR purification: </b><br>M1: 65.9 ng/µl → Store in freezer!<br>M2: 200.6 ng/µl → Store in freezer!<br>M3: 136.8 ng/µl → For digest! → Store in freezer!<br>M4: 49.5 ng/µl → Store in freezer!<br>CB1: 90.8 ng/µl → For digest! → Store in freezer!<br>CB2: 56.7 ng/µl → Store in freezer!<br>P1: 56.2 ng/µl → For digest! → Store in freezer!<br><br><b>Plasmid preparation:</b><br>275.1 PK401 dam- pSBLb4C15-red (dam-):				13.5 ng/µl<br>275.2 PK401 dam- pSBLb4C15-red (dam-):				27.9 ng/µl<br>276. PK401 dam- pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His (dam-): 18.3 ng/µl<br>277. PK401 dam- pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His (dam-): 26.6 ng/µl<br><br><b>Western blot:</b><br>Transfer overnight not complete but continued with anyway<br>Very promising results, bands can be seen around 50 kDa but the ladder is pretty weak so we can’t be absolutely sure. Looks like the J23101 promotor does’nt work.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><b>Blotting and development of constructs:</b><br>43.7 pSB1C3-B0034-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>115. pSB3K3-J23101-B0034-His-TAL<br><br><h2>Other experiments:</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>New buffers for western blot made </b></div>'
+ }
+      else if(id == 'd2013815')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-15</h1><br><b>Name of participants: </b> Christoffer Ahlström, Stephanie Herman, Alona Nyberg, Mikael Strandgren, Alexander, Malin, Pontus, Niclas, Christoffer, Sabri, Hampus.E, Karl H, Ken B, Kristoffer L, Victor S, Marcus H., Peter C, Lovisa P, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Digestions:</b><br>Erythromycin resistance cassette from pLUL631 (SacI, Tango buffer)<br>Bleomycin resistance cassette (SalI, FD buffer)<br><br><b>O/N Screening glycerolstock:</b><br>110.pEL3S17-K206001-B0034-IspA<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Re-streak:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>184.pSB1C3-B0034-CrtZ<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>110.pEL3S17-K206001-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Plasmid prep:</b><br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br><br><b>Transformation:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>292. PK401 dam- pSBLb4C15-CP1-mCherry (dam-)<br>294. PK401 dam- pSBLb4C15-CP8-mCherry (dam-)<br><br><b>Ligations:</b><br>pSBLB4C15 and Bleomycin resistance cassette cut with SacI and SalI<br><br><b>Spreading of strains:</b><br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br><i>E. coli PK401 dam-:</i><br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><b>Screening PCR and gel electrophoresis</b><br><br><h2Results:</h2><br><b>Yesterdays buffers for western blot resulted in smear. </b><br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>47 Chloramphenicol<br>47 Without antibiotics<br><br><b>Testing yesterdays buffers for western blot.</b><br> <br><b>New separation- and stacking buffers for western blot with a factor 1000 concentration of Tris.</b></div>'
+ }
+      else if(id == 'd2013816')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-16</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Mikael Strandgren, Alexander, Pontus, Nils, Christoffer, Sabri, Hampus.E, Kristoffer L, Victor S, Marcus H, Karl H, Theodor L, Peter C<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>O/N Screening & glycerolstock:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>184.pSB1C3-B0034-CrtZ<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>2 74.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br><br><b>Digestion</b><br>Erythromycin cassette amplicon from pLUL631 (SalI)<br><br><b>Ligation</b><br>pSB1C3-pemp<br>298. pSBLb4E15-red (E-cassette amplicon from pLUL631 with pSBLb4C15 (SacI + SalI))<br>297. pSBLb4B15-red (Bleomycin cassette with pSBLb4C15 (SacI + SalI))<br><br><b>Plasmid preparation:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br><br><b>O/N:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS<br>204.3 pEL3S17-J23110-B0034-HIS-STS<br>204.4 pEL3S17-J23110-B0034-HIS-STS<br>254.1 pSB1C3-gus<br>254.2 pSB1C3-gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>255.3 pSBLB4C15-pemp<br>255.4 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>257.3 pSB1C3-pemp<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Transformation:</b><br>297. PK401 dam- pSBLb4B15-red <br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>155pSB1C3-j23110-RBS-meffRed<br>156pSB1C3-j23106-RBS-meffRed<br>157pSB1C3-j23110-RBS-spisPink<br>158pSB1C3-j23106-RBS-spisPink<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>247pSB1C3-J23110-RBS-tsPurple<br>248pSB1C3-J23106-RBS-tsPurple<br>249pSB1C3-J23110-RBS-scOrange<br>250pSB1C3-J23106-RBS-scOrange<br>251pSB1C3-J23110-RBS-fwYellow<br>252pSB1C3-J23106-RBS-fwYellow<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><h2>Results:</h2><br><b>Plasmid prep:</b><br>204.1 pEL3S17-J23110-B0034-HIS-STS: 9.7 ng/µl<br>204.3 pEL3S17-J23110-B0034-HIS-STS: 11.9 ng/µl<br>204.4 pEL3S17-J23110-B0034-HIS-STS: 7.6 ng/µl<br>254.1 pSB1C3-gus: 6.7 ng/µl<br>254.2 pSB1C3-gus: 11.5 ng/µl<br>255.1 pSBLB4C15-pemp: 9.9 ng/µl<br>255.2 pSBLB4C15-pemp: 6.3 ng/µl<br>255.3 pSBLB4C15-pemp: 5.0 ng/µl<br>255.4 pSBLB4C15-pemp: 4.6 ng/µl<br><i>Since the concentrations were so low a new set of overnight cultures were prepared.</i><br><br><b>Spectophotometry:</b><br>Inconclusive results.<br><br><h2>Characterization experiments</h2><br><b>Characterization of Resveratrol production through spectroscopy and HPLC</b><br><br><b>Testing of new buffers for western blot.</b><br><br><h2>Other experiments</h2><br><b>Making MRS-broth (2x600 ml)</b><br><br><b>Making LB-agar plates:</b><br>173 Chloramphenicol <i>(chloramphenicol was forgotten in 26 of the plates, but they had already been mixed with 25 others, so also these had to be discarded. 5 plates of doubtful antibiotic content had already been used by the carotenoid group, they were probably ones with ab. E. colis containing pSB3K3 were spread on some of the mixed plates.)</i><br>28 Kanamycin</div>'
+ }
+      else if(id == 'd2013817')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-17</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Sabri, Hampus.E, Kristoffer L, Viktor @, Marcus H. Ken B-A, Karl H, Theodor L<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry 58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Restreak</b><br>254.3 pSB1C3-Gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>297. pSBLb4B15-red <br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 <br>225: pEL3S17-J23101-B0034-TAL-B0034-4CL-B0034-STS<br>295: pSB1C3-J23110-TAL-B0034-4Cl-B0034-STS<br><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus<br>254.2 pSB1C3-Gus<br>255.1 pSBLB4C15-pemp<br>255.2 pSBLB4C15-pemp<br>257.1 pSB1C3-pemp<br>257.2 pSB1C3-pemp<br>184.pSB1C3-B0034-CrtZ<br>280. pSB1K3-J23110-B0034-aeBlue<br>281. pSB3K3-J23110-B0034-amilCP<br>282. pSB3K3-J23110-B0034-asCP<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin (X,P)<br><br><b>Ligation:</b><br>147.pSB1C3-B0034-Thioredoxin, 91.BBa_J61002 (amp)-J23106, 36.pEL3S17-red<br>---> 300.pEL3S17-J23106-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 92.BBa_J61002 (amp)-J23110, 36.pEL3S17- red<br>---> 301.pEL3S17-J23110-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin, 103.pSB1K3-J23101, 36.pEL3S17-red<br>---> 299.pEL3S17-J23101-B0034-Thioredoxin<br><br><b>Transformation:</b><br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Plasmid preparation:</b><br>254.1 pSB1C3-Gus 110 ng/uL<br>254.2 pSB1C3-Gus 132 ng/uL<br>255.1 pSBLB4C15-pemp156 ng/uL<br>255.2 pSBLB4C15-pemp 158 ng/uL<br>257.1 pSB1C3-pemp 146 ng/uL<br>257.2 pSB1C3-pemp 140 ng/uL</div>'
+ }
+      else if(id == 'd2013818')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-18</h1><br><b>Name of participants: </b>Anders Edlund, Alexander, Pontus, Kristoffer L, Marcus H.<br><br><h2>Ongoing constructs:</h2><br><h2>E. coli:</h2><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>ON:</b><br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br>pSBLB4C15-red<br>pSBLB4C15-red dam-<br><br><b>Restreak:</b><br>68.1 pSB3K3-B0034-BFP<br>68.2 pSB3K3-B0034-BFP<br>68.4 pSB3K3-B0034-BFP<br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.pSB1C3-B0034-Thioredoxin<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His <br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His <br>179 pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His <br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His <br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.pSB3K3-K206000-B0034-IspA <br>268.pSB3K3-K206001-B0034-IspA <br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.pEL3S17-J23106-B0034-Thioredoxin<br><br><b>PCR:</b> <br>134. pSB1C3-B0034-CBP<br>254. pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Digest:</b><br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Ligation:</b><br>4.pSB1A3-red<br>7.pSB3K3-red<br>36.pEL3S17-red<br>91.j23106<br>92.j23110<br>103.pSB1K3-j23101<br>125. pSB1C3-ZCD-His<br>127. pSB1A3-B0034-UGTCs2-His<br>129.pSB1C3-Idi(mut) <br>184.pSB1C3-B0034-CrtZ<br><br><b>Plasmidprep:</b><br>184.pSB1C3-B0034-CrtZ<br><br><b>Transformation:</b><br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>302. idi (mut)-B0034-Ispa<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>305.pSB1A3-j23110-B0034-CrtZ<br><br><b>O/N screening:</b><br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><h2>Results:</h2><br><b>Failed:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His <br>184.pSB1C3-B0034-CrtZ<br>202pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His <br>272 pSB1K3-B0034-Thioredoxin-ZCD-His <br>273 pSB3T5-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His <br>274 pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His</div>'
+ }
+      else if(id == 'd2013819')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-19</h1><br><b>Name of participants: </b>Anton Berglund, Viktor Blomkvist, Mikael Strandgren, Stephanie Herman, Kristoffer L, Marcus H, Karl H, Theodor L, Emil M, Victor S, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Screening</b><br>134.1 pSB1C3-CBP<br>254 pSB1C3-gus<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.3 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.2 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI<br>254.2 pSB1C3-gus: XbaI & PstI<br>255.1 pSBLB4C15-pemp: EcoRI & PstI<br>255.2 pSBLB4C15-pemp: EcoRI & PstI<br>257.1 pSB1C3-pemp: EcoRI & PstI<br>257.2 pSB1C3-pemp: EcoRI & PstI<br><br><b>Frozen stock:</b><br>134.1 pSB1C3-CBP<br>275. pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>1 ml overnight culture:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red<br>111.7(2) pSBLB4C15-red<br>134.1 pSB1C3-CBP<br>275 pSBLB4C15-red (dam-)<br>288.1 pSB1C3-pem<br>288.2 pSB1C3-pem<br>288.4 pSB1C3-pem<br>289.1 pSB1C3-mCherry<br>289.3 pSB1C3-mCherry<br>289.4 pSB1C3-mCherry<br><br><b>Overnight culture:</b><br>Lb11.2 plantarum 36E-no plasmid<br><br><b>Phusion PCR:</b><br>usp45 (synthesized signal peptide from Lactococcus lactis)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>digest</b><br>36. pEL3S17-red<br><br><b>Ligation</b><br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results:</h2><br><b>Screening</b><br>134.1 pSB1C3-CBP: showed the correct band!<br>254 pSB1C3-gus: showed a very weak band, not sure if correct!<br>288.1 pSB1C3-pem: showed the correct band!<br>288.2 pSB1C3-pem: showed the correct band!<br>288.3 pSB1C3-pem: did not show the correct band!<br>288.4 pSB1C3-pem: showed the correct band!<br>289.1 pSB1C3-mCherry: showed the correct band!<br>289.2 pSB1C3-mCherry: did not show the correct band!<br>289.3 pSB1C3-mCherry: showed the correct band!<br>289.4 pSB1C3-mCherry: showed the correct band.<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His: fail<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His: fail<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His: fail<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His: fail<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: fail<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: fail<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: fail<br><br><b>Digestion & screening:</b><br>254.1 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>254.2 pSB1C3-gus: XbaI & PstI, did not show the correct bands!<br>255.1 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>255.2 pSBLB4C15-pemp: EcoRI & PstI, showed the correct bands!<br>257.1 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br>257.2 pSB1C3-pemp: EcoRI & PstI, showed the correct bands!<br><br><b>Plasmid preparation</b><br>111.7(1) pSBLB4C15-red: 81.7 ng/µl<br>111.7(2) pSBLB4C15-red: 93.9 ng/µl<br>134.1 pSB1C3-CBP: 163.8 ng/µl<br>275 pSBLB4C15-red (dam-): 92.4 ng/µl<br>288.1 pSB1C3-pem: 211.2 ng/µl<br>288.2 pSB1C3-pem: 193.2 ng/µl<br>288.4 pSB1C3-pem: 193.9 ng/µl<br>289.1 pSB1C3-mCherry: 230.4 ng/µl<br>289.3 pSB1C3-mCherry: 229.7 ng/µl<br>289.4 pSB1C3-mCherry: 239.7 ng/µl</div>'
+ }
+      else if(id == 'd2013820')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-08-20</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Theodor L, Emil M, Victor S<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Characterisation of the CP promoters with facs:</b><br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>Digestion:</b><br>USP. with E, P<br>191. pSB1A2-fusionGFP with X, P<br>280. pSB1A3-B0034-aeBlue with X, P <br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><b>Making competent Lactobacillus reuteri</b><br>Lb12.1 reuteri DSM 20016 plasmid free: eight<br>Lb12.2 reuteri DSM 20016 plasmid free: eight<br><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: two<br>111.7(1) pSBLB4C15-red: one<br>111.7(2) pSBLB4C15-red: two<br>255.1 pSBLB4C15-pemp: one<br>255.2 pSBLB4C15-pemp: one<br>275 pSBLB4C15-red (dam-): three<br><br><h2>Results:</h2><br><b>Electroporation</b><br>Lb3.2 reuteri DSM 20016 pVS2: one of the two survived<br>111.7(1) pSBLB4C15-red: survived<br>111.7(2) pSBLB4C15-red: one of the two survived<br>255.1 pSBLB4C15-pemp: survived<br>255.2 pSBLB4C15-pemp: survived <br>275 pSBLB4C15-red (dam-): two of the three survived</div>'
+ }
+      else if(id == 'd2013821')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-08-21</h1><br><b>Name of participants: </b>Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Alexander, Pontus, Sabri, Malin, Magnus, christoffer, Marcus H, Karl H, Theodor L, Emil M, Victor S, Hampus.E, Lovisa P<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>204(1-4). pEL3S17-J23110-B0034-His-STS<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work: </h2><br><b>Digestion:</b><br>usp45: XbaI & AgeI<br>191.1 pSB1A2-fusionGFP: XbaI, PstI<br>191.1 pSB1A2-fusionGFP: NgoMIV & PstI<br>275. pSBLB4C15-red (dam-): EcoRI & PstI<br>289.1 pSB1C3-mCherry: NgoMIV & PstI<br>289.1 pSB1C3-mCherry: XbaI, PstI<br>297.1 pSBLB4B15-red (dam-): EcoRI & PstI<br><br><b>Ligation:</b><br>pSBLB4B15-CP11-fusionGFP (297.1+63.2+191.1)<br>pSBLB4B15-CP30-fusionGFP (297.1+65.1+191.1)   		 <br>pSBLB4B15-CP11-usp45-fusionGFP (297.1+63.2+usp45+191.1)   <br>pSBLB4B15-CP30-usp45-fusionGFP (297.1+65.2+usp45+191.1)    <br>pSBLB4B15-CP11-usp45-mCherry (297.1+63.2+usp45+289.1)   	 <br>pSBLB4B15-CP30-usp45-mCherry (297.1+65.2+usp45+289.1)   	 <br>pSB1C3-usp45 (1+usp45)<br>pSB1C3-usp45 (6+usp45)<br>pSBLb4C15-CP1-B0034-BFP<br>pSBLb4C15-CP8-B0034-BFP<br>pSBLb4C15-CP11-B0034-BFP<br>pSBLb4C15-CP29-B0034-BFP<br>pSBLb4C15-CP30-B0034-BFP<br>pSBLb4C15-CP41-B0034-BFP<br>pSBLb4C15-CP44-B0034-BFP<br>pSBLb4C15-CP11-aeblue<br>pSBLb4C15-CP30-aeblue<br>pSBLb4B15-CP11-mcherry<br>pSBLb4B15-CP30-mcherry<br>pSBLb4B15-CP11-tspurple<br>pSBLb4B15-CP30-tspurple<br>pSBLb4B15-CP11-amilCP<br>pSBLb4B15-CP30-amilCP<br>pSBLb4B15-CP11-amilGFP<br>pSBLb4B15-CP30-amilGFP<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>265.pSB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.pSB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Transformation:</b><br>108.pEL3S17-J23106-B0034-IspA<br>141.pRL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br>204(1-4)<br> 	<br><b>Re-streak:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Screen/ ON:</b><br>219.pSB1C3-Miraculin<br>304.pSB1A3-J23106-B0034-CrtZ<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>280. pSB1A3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP <br><br><b>Plasmid prep. </b><br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br><br><b>Mutagenesis PCR:</b><br>336. pSB1C3-spisPink<br>337. pSB1C3-scOrange<br>338. pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (20µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R<br><br><b>Cleaning of electroporation cuvettes</b></div>'
+ }
+      else if(id == 'd2013822')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-08-22</h1><br><b>Name of participants: </b>Anders Edlund, Anton Berglund, Mikael Strandgren, Viktor Blomkvist, Stephanie Herman, Alona Nyberg, Alexander, Pontus, Sabri, Malin, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>70. pSB3K3-CP11-B0034-BFP<br>71. pSB3K3-CP29-B0034-BFP<br>72. pSB3K3-CP30-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>74. pSB3K3-CP44-B0034-BFP<br>111. pSBLb4C15-red<br>134. pSB1C3-CBP<br>139. pSB3K3-J23101-B0034-BFP<br>233. pSBLb4C15-CP1-B0032-BFP<br>234. pSBLb4C15-CP8-B0032-BFP<br>235. pSBLb4C15-CP11-B0032-BFP<br>236. pSBLb4C15-CP29-B0032-BFP<br>237. pSBLb4C15-CP30-B0032-BFP<br>238. pSBLb4C15-CP41-B0032-BFP<br>239. pSBLb4C15-CP44-B0032-BFP<br>254. pSB1C3-gus<br>255. pSBLb4C15-pemp<br>256. pSBLb4C15-pem<br>257. pSB1C3-pemp<br>267 pSB1C3-pemp<br>288. pSB1C3-pem<br>289. pSB1C3-mCherry<br>290. pSB1C3-CBP<br>291. pSBLb4C15-CP1-mCherry<br>293. pSBLb4C15-CP8-mCherry<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15)<br><br><b>E. coli PK401 dam-:</b><br>275.2 pSBLb4C15-red<br>276 pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>277. pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>292. pSBLb4C15-CP1-mCherry<br>294. pSBLb4C15-CP8-mCherry<br><br><h2>Todays work:</h2><br><b>Competent cell preparation:</b><br>Lb11.1 plantarum 36E no plasmid<br>Lb12.1 reuteri DSM 20016 no plasmid<br>Lb12.2 reuteri DSM 20016 no plasmid<br><br><b>Digest:</b><br>147.B0034-Thioredoxin (E, S)<br>147.B0034-Thioredoxin (X,P)   <br>27.pEL3K16-red (E,P)<br>125.pSB1C3-ZCD-His (X,P)<br><br><b>Ligation:</b><br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Transformation:</b><br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><strike>3.2. pVS2 (died in electroporation)<br>310. pSBLB4B15-CP11-fusionGFP (died in electroporation)<br>318. pSBLB4B15-CP11-mCherry (died in electroporation)<br>320. pSBLB4B15-CP11-amilGFP (died in electroporation)<br>313. pSBLB4B15-CP30-usp45-fusionGFP (died in electroporation)<br>317. pSBLB4B15-CP30-tsPurple (died in electroporation)<br>331. pSBLB4C15-CP41-BFP (died in electroporation)<br>332. pSBLB4C15-CP44-BFP (died in electroporation)</strike><br><b>+ other E. coli transformations</b><br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>Screen/ ON:</b><br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1A3-j23101-B0034-CrtZ<br>304.pSB1A3-j23106-B0034-CrtZ<br><br><b>Plasmid prep. / Seq. prep.</b><br>147.pSB1C3-B0034-Thioredoxin<br>184.pSB1C3-B0034-CrtZ<br><br><b>Gel:</b><br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>207.CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><b>Phusion PCR:</b><br>->219.pSB1C3-Miraculin<br><br><h2>Results:</h2><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp: Problem with PCR-machine, paused at 98*C for 25 min. → re-do.<br>288. pSB1C3-pem:  				--”--<br><br><b>Transformation:</b><br>11.1 Lb. plantarum 36E:<br>3.2 pVS2: survived electroporation<br>3.2 pVS2: died during electroporation<br>310. pSBLB4B15-CP11-fusionGFP: died during electroporation<br>311. pSBLB4B15-CP30-fusionGFP: survived electroporation<br>312. pSBLB4B15-CP11-usp45-fusionGFP: survived electroporation<br>313. pSBLB4B15-CP30-usp45-fusionGFP: died during electroporation<br>316. pSBLB4B15-CP11-tsPurple: survived electroporation<br>317. pSBLB4B15-CP30-tsPurple: died during electroporation<br><br><b>12.2 Lb. reuteri DSM 20016:</b><br>3.2 pVS2: survived electroporation<br>318. pSBLB4B15-CP11-mCherry: died during electroporation<br>319. pSBLB4B15-CP30-mCherry: survived electroporation<br>320. pSBLB4B15-CP11-amilGFP: died during electroporation<br>321. pSBLB4B15-CP30-amilGFP: survived electroporation<br>322. pSBLB4B15-CP11-amilCP: survived electroporation<br>323. pSBLB4B15-CP30-amilCP: survived electroporation<br>324. pSBLB4C15-CP11-aeBlue: survived electroporation<br><br><b>12.1 Lb. reuteri DSM 20016:</b><br>325. pSBLB4C15-CP30-aeBlue: survived electroporation<br>326. pSBLB4C15-CP1-BFP: survived electroporation<br>327. pSBLB4C15-CP8-BFP: survived electroporation<br>328. pSBLB4C15-CP11-BFP: survived electroporation<br>329. pSBLB4C15-CP29-BFP: survived electroporation<br>330. pSBLB4C15-CP30-BFP: survived electroporation<br>331. pSBLB4C15-CP41-BFP: died during electroporation<br>332. pSBLB4C15-CP44-BFP: died during electroporation<br><br><h2>Characterization:</h2><br><b>Western blot analysis (SDS-page) on constructs:</b><br>115. pSB3K5-J23101-B0034-His-TAL<br>116. pSB3K3-J23110-B0034-His-TAL<br>117. pSB3K3-CP1-B0034-His-TAL<br>119. pSB1C3-B0034-4CL (from 15) (neg.contr)<br><br><h2>Other experiments:</h2><br><b>Phosphorylation of primers (60 µl of each):</b><br>Pem-mutE_F<br>Pem-mutE_R<br>Pem-mutS_F<br>Pem-mutS_R</div>'
+ }
+      else if(id == 'd2013823')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-08-23</h1><br><b>Name of participants: </b>Anton Berglund, Stephanie Herman, Viktor Berglund, Mikael Strandgren, Anders Edlund, Alexander, Pontus, Sabri, Malin, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>E. coli (D5α):</b><br>111.7 pSBLb4C15-red<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>111.7 pSBLb4C15-red (cut with E and P) (to get reference/examine cutsites)<br><br><b>Mutagenesis PCR:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br>Touchdown with Tm1+2 and Tm1+4.<br><br><b>Transformation:</b><br>102.pSB1C3-CrtE<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>Re-streak:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Screening O/N:</b><br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>O/N:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><b>Glycerol stock Plasmidprep/sekvensprep:</b><br>108.pEL3S17-J23106-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>184.pSB1C3-B0034-CrtZ<br>299.pEL3S17-J23101-B0034-Thioredoxin<br><br><h2>Results:</h2><br><b>Gel electrophoresis:</b><br>257. pSB1C3-pemp with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>288. pSB1C3-pem with Pem-mutE_F & Pem-mutE_R: Unspecific binding, nc ok!*<br>111.7 pSBLb4C15-red (cut with E and P): No band<br><i>	*→ redo mutagenesis PCR with higher temperatures</i></div>'
+ }
+      else if(id == 'd2013824')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-08-24</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Berglund, Alexander, Pontus, Sabri, Magnus, Marcus H, Karl H, Hampus.E, Lovisa P, Kristoffer L, Emil M<br><br><h2>Ongoing constructs:</h2><br><b>Escherichia coli</b><br>257. pSB1C3-pemp<br>288. pSB1C3-pem<br>136. pSB1C3-B0034-USP<br>pSB1C3-B0034-usp45<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><b>Lactobacillus reuteri:</b><br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br><br><b>Lactobacillus plantarum:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue <br><br><b>Digest:</b><br>200.pSB1C3-B0034-asCP E,X<br>148.pSB1C3-RBS-gfasPurple E,X<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 E,X<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R (EcoRI)<br><br><b>Ligation:</b><br>200.pSB1C3-B0034-asCP+91.BBa_J61002 (amp)-J23106→<br>→ 343.J23106-B0034-asCP<br>148.pSB1C3-RBS-gfasPurple+91.BBa_J61002 (amp)-J23106→<br>→ 160.j23106-RBS-gfasPurple<br>148.pSB1C3-RBS-gfasPurple+92.BBa_J61002 (amp)-J23110→<br>→ 159j23110-RBS-gfasPurple<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1+6.pSB1K3-red→<br>→215.pSB1K3-pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><b>Transformation:</b><br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>343.pSB1C3-J23106-B0034-asCP<br>160.pSB1C3-j23106-RBS-gfasPurple<br>159.pSB1C3-j23110-RBS-gfasPurple<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>336.pSB1C3.spisPink<br>337.pSB1C3.scOrange<br>284.pSB1C3.B0034-aeBlue<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>284. pSB1C3-B0034-aeBlue<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br><br><b>O/N screening:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>136.<br>312<br>313<br>315<br>321<br>325<br>327<br>332<br>317<br><br><b>O/N:</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Plasmid prepp & sequence prepp</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br><br><b>Gel electrophoresis:</b><br>Lb31. plantarum 36E pVS2<br>Lb32. plantarum 36E pSBLB4B15-CP30-fusionGFP<br>Lb33. plantarum 36E pSBLB4B15-CP11-usp45-fusionGFP<br>Lb34. plantarum 36E pSBLB4B15-CP11-tsPurple<br>Lb35. reuteri DSM 20016 pVS2<br>Lb36. reuteri DSM 20016 pSBLB4B15-CP30-mCherry<br>Lb37. reuteri DSM 20016 pSBLB4B15-CP30-amilGFP<br>Lb38. reuteri DSM 20016 pSBLB4B15-CP11-amilCP<br>Lb39. reuteri DSM 20016 pSBLB4B15-CP30-amilCP<br>Lb40. reuteri DSM 20016 pSBLB4C15-CP11-aeBlue<br>Lb41. reuteri DSM 20016 pSBLB4C15-CP30-aeBlue<br>Lb42. reuteri DSM 20016 pSBLB4C15-CP1-BFP<br>Lb43. reuteri DSM 20016 pSBLB4C15-CP8-BFP<br>Lb44. reuteri DSM 20016 pSBLB4C15-CP11-BFP<br>Lb45. reuteri DSM 20016 pSBLB4C15-CP29-BFP<br>Lb46. reuteri DSM 20016 pSBLB4C15-CP30-BFP<br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><i>+ E. coli transformations</i><br><br><b>PCR-purification:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><b>DpnI:</b><br>257. pSB1C3-pemp (two tubes) with Pem-mutE_F & Pem-mutE_R<br>288. pSB1C3-pem (two tubes) with Pem-mutE_F & Pem-mutE_R<br><br><h2>Results:</h2><br><b>Transformations:</b><br>All transformations spread on Bleomycin/zeocin have grown! If they are the right lactobacilli with the right plasmid remains to be seen, there seem to be a lot of contamination in the form of “clouds” covering all plates. Yet still there are larger colonies that very well could be what we want. Maybe the antibiotics are not efficient enough, we will try to spread normal plasmid free reuteri from frozen stock on the plates to investigate… We’ll also try a colony screening today with VF2 and VR. <br>Nothing grew on chloramphenicol plates, not even the positive control pVS2, though it grew on erythromycin both when transformed into reuteri and plantarum. <br><br><b>Gel electrophoresis:</b><br>All Pem/Pemp mutagenesises showed bands at the right lengths (~2700 bp), although they were a bit smudgy - weakly stretching backward toward the wells. The digests of Pem simply showed one band, a weaker counterpart of the uncut PCR-product. We suspect the digest didn’t go as supposed, maybe due to the PCR buffer. <br>The lactobacillus colony screenings showed up empty, perhaps the cells didn’t lyse? Or didn’t contain plasmids with VF and VR… We will continue to investigate the resistance problems, and await the sequencing results of the insertsite.<br>The screenings of e. coli transformations done thursday also gave us an empty gel, without even ladders. Possibly the gel had to shallow wells.</div>'
+ }
+      else if(id == 'd2013825')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1><br><b>Name of participants:</b> Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin<br><br><b>Ligate:</b><br>147.pSB1C3-B0034-Thioredoxin+CP1→<br>→ 346.pSB1C3-CP1-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP8→<br>→347.pSB1C3-CP8-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP41→<br>→348.pSB1C3-CP41-B0034-Thioredoxin<br><br><b>Transformation:</b><br>1.pSB1C3-Red (competens test)<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ<br>336.pSB1C3-spisPink<br>337pSB1C3-scOrange<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348.pSB1C3-CP41-B0034-Thioredoxin<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br><br><b>Re-streak:</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Sequenceprep:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br></div>'
+ }
+      else if(id == 'd2013826')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1><br><b>Name of participants: </b>Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br>Slovenian 4CL with His-primers<br><br><b>Gel electrophoresis:</b><br>Erythromycin-cassette PCR-product from Lb2.1 pLUL631<br>Erythromycin-cassette PCR-product from Lb2.2 pLUL631<br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br><br><b>Assembly & transformation:</b><br>349<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Re streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>Plasmidprepp:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>O/N screen</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Screening:</b><br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348. pSB1C3-CP41-B0034-Thioredoxin<br><br><b>O/N:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades)<br><br><b>Re-streak</b><br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades)<br><br><b>Sekvensprep:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Ligation:</b><br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → <br>→215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Results:</h2><br><b>Gel electrophoresis: </b><br>Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly<br>Erythromycin-cassette PCR-product:  			--”--<br>Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed<br>77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp.<br>269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made.<br><br><h2>Characterization:</h2><br><b>Western blot sample preparation:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparing MRS-agar</b><br><br><b>Preparing low salt LB-agar</b></div>'
+ }
+      else if(id == 'd2013827')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday  2013-08-27</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>O/N:</b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br><br><b>Transformation:</b><br>Chromoproteins into pSB3K3<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1K3-CP1-B0034-Thioredoxin<br>347.pSB1K3-CP8-B0034-Thioredoxin<br>348.pSB1K3-CP41-B0034-Thioredoxin<br><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br><br><b>Screening PCR: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br><br><b>Sekvensprep:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>284. pSB1C3-B0034-aeBlue<br><br><b>Re-streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>PCR:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures Tm1 and +5°C (with touchdown))</i><br><br><b>Streaking of plasmid free stock on test plates:</b><br><i>- e. coli (10. and 60.3) onto erythromycin LB-plates:</i><br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><i>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:</i><br>50 µg/ml<br>100 µg/ml<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp<br>77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp<br><br><h2>Other experiments</h2><br><b>Preparing test plates, to determine minimum antibiotic concentration:</b><br>Lb-erythromycin:<br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><br><b>MRS-zeocin:</b><br>50 µg/ml<br>100 µg/ml</div>'
+ }
+      else if(id == 'd2013828')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday  2013-08-28</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>58. pSB1C3- B0034-IspA<br>62.pSB1C3-CP8<br>95. pSB1C3-K206001<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>129.pSB1C3-Idi(mut) <br>140.pSB3T5-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>193.pSB1C3-amilGFP<br>194.pSB1C3-B0034-amilCP<br>195.pSB1C3-cjBlue<br>196.pSB1C3-eforRed<br>197.pSB1C3-aeBlue<br>198.pSB1C3-B0034-amilGFP<br>199.pSB1C3-amajCFP<br>200.pSB1C3-B0034-asCP<br>201. E.Coli. Nissle - CrtY-CrtI<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>219.pSB1C3-Miraculin<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>226. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2012 years kit)<br>227. pSB1C3-pBAD/AraC-RBS-Luciferin (from 2013 years kit)<br>228. pEL3A17-Blue<br>229. pEL3K17-Blue<br>230. pEL3S15-Blue<br>231. pEL3C17-Blue<br>253. pEL3S17-J23101-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>267.psB3K3-K206000-B0034-IspA<br>268.psB3K3-K206001-B0034-IspA<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>274.pSB3K3-B0034-Thioredoxin-ZCD-His-B0034-UGTCs2-His<br>282.pSB3K3-J23110-B0034-amilCP<br>283.pSB3K3-J23110-B0034-asCCP<br>299.pEL3S17-J23101-B0034-Thioredoxin<br>300.pEL3S17-J23106-B0034-Thioredoxin<br>301.pEL3S17-J23110-B0034-Thioredoxin<br><br><h2>Todays work</h2><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>77. pSB4C15-red (annealing at Tm1): <br>77. pSB4C15-red (annealing at Tm1+5): <br><br><b>Ligation: </b><br>352. pSBLbC3-red (pEL4C15 (ClaI) + pVS2 (ClaI))<br>108.pEL3S17-J23106-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-His<br>141.pEL3S17-J23101-B0034-UGTCs2-His<br>142.pEL3S17-CP1-B0034-UGTCs2-His<br>143.pEL3S17-CP8-B0034-UGTCs2-His<br><br><b>PCR: </b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R (Tm1)<br><br><b>Restreak</b><br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br><br><b>Screening O/N:</b><br>109.pEL3S17-K206000-B0034-IspA <br>110.pEL3S17-K206001-B0034-IspA <br>147.B0034-Thioredoxin<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His <br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His <br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His <br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His <br>267.psB3K3-K206000-B0034-IspA <br>268.psB3K3-K206001-B0034-IspA <br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His <br>300.J23106-B0034-Thioredoxin<br>202.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>207.pSB4C5-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br><br><br><b>Digest</b><br>36. pEL3S17-red<br><br><b>Subclone:</b><br>280. pSB1A3-B0034-aeBlue à 284. pSB1C3-B0034-aeBlue<br>281. pSB1K3-J23110-B0034-aeBlue à 285. pSB1C3-J23110-B0034-aeBlue<br>282. pSB3K3-J23110-B0034-amilCP à 286. pSB1C3-J23110-B0034-amilCP<br>283. pSB3K3-J23110-B0034-asCP à 287. pSB1C3-J23110-B0034-asCP<br><br><br><b>Two step Assembly:</b><br>92. BBa_J61002-J23110,  149. pSB1C3-RBS-meffRed à 155. pSB1C3-J23110-RBS-meffRed<br>91. BBa_J61002-J23106,  149. pSB1C3-RBS-meffRed à 156. pSB1C3-J23106-RBS-meffRed<br>92. BBa_J61002-J23110, 150. pSB1C3-RBS-spisPink à 157. pSB1C3-J23110-RBS-spisPink<br>91. BBa_J61002-J23106, 150. pSB1C3-RBS-spisPink à 158.pSB1C3-J23106-RBS-spisPink<br>92. BBa_J61002-J23110, 148. pSB1C3-RBS-gfasPurple à 159.pSB1C3-J23110-RBS-gfasPurple<br>91. BBa_J61002-J23106, 148 pSB1C3-RBS-gfasPurple à 160.pSB1C3-J23106-RBS-gfasPurple<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>5 µg/ml: Growth <br>10 µg/ml: Growth<br>20 µg/ml: Growth<br>50 µg/ml: Still some growth, but less → do new plates with higher conc. and try 50 µg/ml-plates from Erik Gullberg in case our antibiotic has lost efficiency.<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:<br>50 µg/ml: Growth<br>100 µg/ml: Growth (Plantarum grew less, but did so also on antibiotic free plates.)<br>- lactobacillus (Lb4., Lb11. and Lb12.) onto antibiotic-free MRS-plates:<br>Everything grew on Ab-, plantarum slightly less.<br><br><b>Gel electrophoresis: </b><br>351.1 pSB1C3-pemp-mut1: A weak band at ~1000 bp<br>351.2 pSB1C3-pemp-mut1: Nothing<br>77. pSB4C15-red (annealing at Tm1): Nothing → do a new PCR at TM1.<br>77. pSB4C15-red (annealing at Tm1+5): Nothing. <br>202. pSB4C5-RBS-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268: Failed<br>203. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1: Failed<br>207. pSB4C5-RBS-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1: Failed<br><br><b>Re-streak:</b><br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>LB-erythromycin:<br>100 µg/ml<br>200 µg/ml</div>'
+ }
+      else if(id == 'd2013829')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday  2013-08-29</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Blomkvist, Alona Nyberg, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>353. pSBLb4E15<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>353. pSBLb4E15<br><br><b>Plasmidprep:</b><br>223<br>346<br><br><h2>Results</h2><br><b>Streaking of plasmid free stock on test plates:</b><br>- e. coli (10. and 60.3) onto erythromycin LB-plates:<br>100 µg/ml: Weak growth<br>200 µg/ml: Possibly no growth, let them “grow” some more. <br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparation of new test-plates:</b><br>MRS-Zeocin:<br>250 µg/ml<br></div>'
+ }
+      else if(id == 'd2013830')
+ {
+	ds = '<div id="dairy-text"><h1>Friday  2013-08-30</h1><br><b>Name of participants: </b>Mikael Strandgren, Viktor Björklund, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>77. pSB4C15-red <br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>19.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>Lb9. faecalis JH2-2 pAMbeta<br><br><h2>Todays work</h2><br><b>Gel extraction:</b><br>77. pSB4C15-red (annealing at Tm1):  <br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R*<br>*Since the product from monday disappeared.<br><br><b>PCR purification:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori<br><br><b>Digestion:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori (PCR product)(XbaI and BglII)<br>77. pSB4C15-red (PCR product) (NheI and BglII) <br><br><b>Ligation:</b><br>169. pSBLbEcC2-red (shuttle-vector with pAMbeta and e. coli replicon)<br><br><h2>Results</h2><br><b>Western blot:</b> <br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd2013831')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday  2013-08-31</h1><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br>386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1)<br><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation)<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'
+ }
+      else if(id == 'd201391')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-01</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red<br>406. pSBLbC3-red (dam-)<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br>406. pSBLbC3-red (dam-)<br><br><b>Screening:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2)<br><br><b>Re-streak:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br><br><h2>Results</h2><br>First screening failed (weird gel)<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201392')
+ {
+	ds = '<div id="dairy-text"><h1>Monday  2013-09-02</h1><br><br><b>Name of participants:</b> Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br><b>O/N:</b><br>169. pSBLbEcC2-red<br><br><b>Mutagenesis PCR:</b><br>111.7 pSBLb4C15-red *<br>*with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night.<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br>352. pSBLbC3-red*<br>*New attempts at ligation, just in case.<br><br><h2>Results: </h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: No bands<br><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201393')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br>Frozen stock and plasmid preparation:<br>169. pSBLbEcC2-red<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red* <br><i>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</i><br><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br><i>*New attempts at ligation, just in case.</i><br><br><b>Sequencing preparation:</b><br>169. pSBLbEcC2-red<br><br><h2>Characterization experiments:</h2><br><b>Western blot sample preparation on construct:</b><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Results: </h2><br><b>Frozen stock and plasmid preparation:</b><br>169. pSBLbEcC2-red: 44.2 ng/µl<br>169. pSBLbEcC2-red: 47.8 ng/µl<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'
+ }
+      else if(id == 'd201394')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'
+ }
+      else if(id == 'd201395')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work</h2><b>First day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201396')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><b>Second day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201397')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><b>Third day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'
+ }
+      else if(id == 'd201398')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: 			“ <br>414. pSB1C3-CP29-amilCP 			“<br>415. pSB1C3-CP11-amilCP 			“<br>417. pSB1C3-CP29-aeBlue 			“<br>418. pSB1C3-CP11-aeBlue			“<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'
+ }
+      else if(id == 'd201399')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1><br><b>Name of participants: </b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Digest</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br> <br><b>Ligation</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Fourth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br></div>'
+ }
+      else if(id == 'd2013910')
+ {
+	ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1><br><b>Name of participants:</b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Re-streak</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br><br><b>Fifth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br></div>'
+ }
+      else if(id == 'd2013911')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1><br><b>Name of participants: </b>Sabri<br><br><h2>Todays Work:</h2><br><b>O/N:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+      else if(id == 'd2013912')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1><br><b>Name of participants:</b> Sabri<br><br><h2>Todays Work:</h2><br><b>Plasmid prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br> <br><b>Sequence prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+ else if(id == 'd20131013')
+ {
+  ds ='<h1>European Jamboree Lyon end</h1>';
+ }
+ else if(id == 'd20131012')
+ {
+  ds ='<h1>European Jamboree Lyon</h1>';
+ }
+ else if(id == 'd20131011')
+ {
+  ds ='<h1>European Jamboree Lyon start</h1>';
+ }
+ else if(id == 'd2013104')
+ {
+  ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';
+ }
+      else if(id == 'd2013102')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Sequencing preparation:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br></div>'
+ }
+       else if(id == 'd2013101')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs </h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Screening PCR:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Results</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2: <b>Growing</b><br>Lb13. pJP059: <b>Growing</b><br>420. pSBLbE-CP11-amilCP: Not growing<br>435. pSBLbC-CP29-amilCP: Not growing<br>440. pSBLbC-red: Not growing<br>441. pSBLbE-red: <b>Growing</b><br>All negative controls were clear, and the positive controls grew</b>.<br></div>'
+ }
+       else if(id == 'd2013928')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1><br><b>Name of participants:</b> Viktor Blomkvist, Mikael Strandgren, Erik Edlund<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><b>L. reuteri:</b><br>Lb3. pVS2<br><br><b>Lc. lactis:</b><br>Lb13. pJP059<br><br><h2>Todays work</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2<br>Lb13. pJP059<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013927')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Overnight:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br><br><b>Streak from frozen stock (for photos):</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013924')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br><br><h2>Results</h2><br><b>Transformation</b>:<br>No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday.<br></div>'
+ }
+       else if(id == 'd2013921')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist <br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Transformation</b><br><i>To L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2 (positive control)<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>Erythromycin<br>Chloramphenicol <br>Antibiotic-free<br></div>'
+ }
+       else if(id == 'd2013920')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+ else if(id == 'd2013919')
+ {
+  ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';
+ }
+       else if(id == 'd2013918')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1><br><b>Name of participants: </b> Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>O/N:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+        else if(id == 'd2013917')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+         else if(id == 'd2013916')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>440. pSBLbC-red<br><br><b>Assembly and transformation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+ else
+ {
+  ds = 'Nothing happend during this day!';
+ }
+ document.getElementById(id).setAttribute('onclick','closeNote('+y+', '+m+', '+d+');');
+ document.getElementById(id).innerHTML = ds;
+}
+function closeNote(y, m, d)
+{
+ var ds = '';
+ var id = 'd'+y+m+d;
+ var ds = '<h1>'+y+'-'+m+'-'+d+'</h1>';
+ document.getElementById(id).setAttribute('onclick','diry('+y+', '+m+', '+d+');');
+ document.getElementById(id).innerHTML = ds;
+}
 </script>
+</html>

Template:Team:Uppsala/JS/notebook

From 2013.igem.org

Latest revision as of 23:26, 4 October 2013