Team:Paris Saclay/Notebook/July/10

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='''Notebook : July 10'''=
='''Notebook : July 10'''=
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=='''Lab work'''==
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==='''A - Aerobic/Anaerobic regulation system'''===
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==''Summary:''==
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===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
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For regulation system:
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===='''1 - Transformation of BBa_I732019 in DH5α'''====
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*prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing.
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*the terminator transformation of yesterday did not work, a second transformation had been done for it.
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*The transformation for RBS+LacZ+terminator plasmid into competent cells was performed.
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*after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a liquid nutritive medium.
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<br>
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Sheng
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For PSBs sensor system:
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*the ligation products were transformed into competent cells and were cultured on solid medium with their specific antibiotics.
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=='''Lab work'''==
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
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constructing
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===='''Objective : obtaining biobricks in pSB3K3'''====
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===='''1 - Mini and maxi preparation of the culture of BBa_J04450'''====
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Abdou
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{|
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| style="border:1px solid black;padding:5px;background-color:#DE;" |
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Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.
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|}
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Protocol : [[Team:Paris_Saclay/mini_maxi|Mini and maxi preparation]]
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We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
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==='''B - PCB sensor system'''===
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===='''Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein'''====
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===='''1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2'''====
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 +
Abdou
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]]
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<br>
 
{| border="1" align="center"
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|[[Team:Paris Saclay/Notebook/July/9|<big>Previous day</big>]]
|[[Team:Paris Saclay/Notebook/July/9|<big>Previous day</big>]]

Latest revision as of 16:36, 4 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 10

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - Transformation of BBa_I732019 in DH5α

Sheng

Protocol : Bacterial transformation

Objective : obtaining biobricks in pSB3K3

1 - Mini and maxi preparation of the culture of BBa_J04450

Abdou

Transformation of BBa_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations.

Protocol : Mini and maxi preparation

We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002, BphR2 protein

1 - Transformation of ligation of pSB1C3 and BphA1 or BphR1 or BphR2

Abdou

Protocol : Bacterial transformation


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