Team:DTU-Denmark

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Global demand for fixed nitrogen has increased to the point that half the human population now relies on chemical fertilizer to grow their food. While fertilizer is a requirement for modern life, runoff from overfertilized farmland can cause eutrophication.  In the presence of abundant ammonia, algae overgrow and consume the much of the available oxygen in the water.  This results in decreased biodiversity throughout the watershed.  Within Europe, 53% of lakes are eutrophic.
 
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Using two E. coli mutants built with genes from Nitrosomonas europaea and Pseudomonas aeruginosa, we provide a system to reverse nitrogen fixation.  Our mutants consume ammonia and produce nitrous oxide, releases a sustainable source of energy when decomposed into nitrogen and oxygen.  We also provide a prototype of a bioreactor that could be scaled up and deployed in the field to simultaneously clean the water and produce energy.
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==Highlights==
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* We successfully [[Team:DTU-Denmark/Experiment4|characterized ammonia consumption of our AMO transformant (Mutant 1)]].
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* We constructed and characterized a [[Team:DTU-Denmark/pBAD_SPL|synthetic promoter library]] built on the arabinose inducible promoter.
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* We successfully [[Team:DTU-Denmark/HelloWorld|targeted proteins to the periplasm]] in our Hello World pilot project.
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* We [[Team:DTU-Denmark/Modeling|modeled]] several aspects of the project including [[Team:DTU-Denmark/Kinetic_Model|the kinetics of the system]] and [[Team:DTU-Denmark/Reactor_Model|the size of reactor]] required.
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* We conducted several events around [[Team:DTU-Denmark/Human_Practices|human practices]], including a [[Team:DTU-Denmark/Biobrick_Workshop|biobrick workshop]] and a [[Team:DTU-Denmark/IP_and_Synthetic_Biology|discussion on IP and synthetic biology]].
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For more information on our project, please see [[Team:DTU-Denmark/Project| our project details]].  Also be sure to check out our [[Team:DTU-Denmark/Notebook| online lab notebook]].
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Latest revision as of 20:58, 4 October 2013

Highlights

For more information on our project, please see our project details. Also be sure to check out our online lab notebook.

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