Team:UNITN-Trento/Notebook/Labposts/08/38

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(Difference between revisions)

Latest revision as of 09:13, 3 October 2013

{

   "date" : "2013-08-15",
   "author" : "emil",
   "title" : "Emil's (re)begins",
   "content" : "I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the     results:      gel image 
Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the purification protocol with the following results:      results                     GFP 170.5 ng/6micro;lGFP 252 ng/6micro;lPXyl 1110.4 ng/6micro;lPxyl 277.7 ng/6micro;lPxyl 3137 ng/6micro;l                                   Sample                    Quantities                                                     
                    
                                                    
                    
                                                     
                    
                                                     
                    
                                                     
                    
                             
                   
    After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the  digestion protocol.",
   "tags" : "PXyl-GFP"

}

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-15",
+    "date" : "2013-08-15",
-	"author" : "fabio-thomas",
+    "author" : "emil",
-	"title" : " blue light induction, festivity time!",
+    "title" : "Emil's (re)begins",
-	"content" : "<html> after yesterday disastrous PCR, we decided to take another direction: put the terminator B0015 downstream of j23100_YF1_FixJ, then rebuild the complete device.We started from digestion (biobrick cloning protocol) of 500 ng in 25 ul of both the parts (j23100_YF1_FixJ part starting concentration= 234 ng/ul and B0015 starting concentration = 309,9 ng/ul). We deactivated enzymes, added SAP, deactivated it, then started the ligation: following the protocol, we used concentration values for both parts= 20 ng/ul. We made the control sample and three ligation samples (1:1, 1:2, 1:3); we transformed and plated them.Meanwhile I took back the experiments with the complete device, so I repeated the dilution cultures (5 ml this time) and tried it again, with a Blue lamp and a less intense LED induction. Moreover I induced with blue light the “mask plates” ,with the entire device, to see if colonies produce blue color if induced.Furthermore I inoculate some colonies from Viola’s plate containing the part S04617+EFE.</html>",
+    "content" : "<html>I screened 5&micro;l of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the     results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel image|<html> <img src=''/></html>}}<html>Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Promega-PCR-Gel'>purification protocol</a> with the following results:</html>      {{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html>         <center>            <table>                <td>                   <th>Sample</th>                   <th>Quantities</th>                </td>                <td>                    <tr>GFP 1</tr>                    <tr>70.5 ng/6micro;l</tr>                </td>                <td>                    <tr>GFP 2</tr>                    <tr>52 ng/6micro;l</tr>                </td>                 <td>                    <tr>PXyl 1</tr>                    <tr>110.4 ng/6micro;l</tr>                </td>                 <td>                    <tr>Pxyl 2</tr>                    <tr>77.7 ng/6micro;l</tr>                </td>                 <td>                    <tr>Pxyl 3</tr>                    <tr>137 ng/6micro;l</tr>                </td>             </table>           </center>        </html>}}    <html>After that I did a digestion overnight of 3 &micro;l of the GFP1 and the PXyl1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> digestion protocol</a>.</html>",
-	"tags" : " BlueLight_amilCP-B0015- S04617+EFE "
+    "tags" : "PXyl-GFP"
 }