Team:UNITN-Trento/Notebook/Labposts/08/36

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(Difference between revisions)

Latest revision as of 09:12, 3 October 2013

{ "date" : "2013-08-14", "author" : "viola-thomas", "title" : "The triple ligation has grown!", "content" : "Taking a look on the ligation plates the ctrl is empty, on the 1:1 there are some small colony (2/3) a and the 1:2 condition is really full of cononies! Fabio has now to verify if there is really all the complete insert...", "tags" : "pFixK-cI-pLambda-EFE" }

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 {
-	"date" : "2013-08-13",
+	"date" : "2013-08-14",
-	"author" : "fabio",
+	"author" : "viola-thomas",
-	"title" : " blue light induction",
+	"title" : "The triple ligation has grown!",
-	"content" : "<html> today I made some dilutions 1:100 from the inocula, to have 3 cultures (200 ml) for the experiment: one will be induced with blue light, the other two are control sample, the fors exposed to standard light, the last one will stay “alone in the dark”. I started the experiment when the dilution cultures reached 0.7 OD. After 4 ours from the beginning of the induction I took 10 ml from the samples and obtained pellet to see blue pigment production: no difference. The experiment will go on over the night, tomorrow we will hopefully be able to see some difference. Meanwhile I made some inocula from the same plate, in order to repeat the experiment with less volume cultures.</html>",
+	"content" : "<html>Taking a look on the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-13-viola-thomas\">ligation plates</a> the ctrl is empty, on the 1:1 there are some small colony (2/3) a and the 1:2 condition is really full of cononies! Fabio has now to verify if there is really all the complete insert...</html>",
-	"tags" : " BlueLight_amilCP"
+	"tags" : "pFixK-cI-pLambda-EFE"
 }