Team:KU Leuven

From 2013.igem.org

(Difference between revisions)
 
(141 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:KU Leuven/Template:Header}}
{{:Team:KU Leuven/Template:Header}}
 +
{{:Team:KU Leuven/Template:Style}}
{{:Team:KU Leuven/Template:Menu}}
{{:Team:KU Leuven/Template:Menu}}
<html>
<html>
-
<head>
+
<body>
-
<meta charset="utf-8">
+
<div id="container">
-
  <meta name="viewport" content="width=device-width, initial-scale=1.0">
+
<div class="container">
-
<title>iGem</title>
+
-
    <link href="http://www.designingtest.org/iGem/bootstrap.min.css" rel="stylesheet">
 
-
    <link href="http://www.designingtest.org/iGem/bootstrap-responsive.min.css" rel="stylesheet">
 
-
    <link href="http://www.designingtest.org/iGem/style.css" rel="stylesheet">
 
-
<link href="http://www.designingtest.org/iGem/bjqs.css"rel="Stylesheet"/>
 
-
   
 
-
    <!--[if lt IE 9]>
 
-
<script src="http://css3-mediaqueries-js.googlecode.com/files/css3-mediaqueries.js"></script>
 
-
<![endif]-->
 
-
 
-
<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.9.1/jquery.min.js"></script> 
 
-
<script src="http://www.designingtest.org/iGem/raphael-min.js"></script>
 
-
<script src="http://www.designingtest.org/iGem/livicons-1.2.min.js"></script>
 
-
<script src="http://www.designingtest.org/iGem/bjqs.min.js"></script>
 
-
 
-
<!--[if lt IE 8]>
 
-
<script src="http://www.designingtest.org/iGem/json2.min.js"></script>
 
-
<![endif]-->
 
-
 
 
-
  <script>
 
-
var ww = document.body.clientWidth;
 
-
 
-
$(document).ready(function() {
 
-
$('#banner-fade').bjqs({
 
-
        'height' : 200,
 
-
        'width' : 340,
 
-
        'responsive' : true,
 
-
        'showcontrols' : false,
 
-
        'showmarkers' : false,
 
-
        'usecaptions' : false,
 
-
        'animspeed' : 6000
 
-
    });
 
-
 
-
 
-
$(".nav li a").each(function() {
 
-
if ($(this).next().length > 0) {
 
-
$(this).addClass("parent");
 
-
};
 
-
})
 
-
 
-
$(".toggleMenu").click(function(e) {
 
-
e.preventDefault();
 
-
$(this).toggleClass("active");
 
-
$(".nav").toggle();
 
-
});
 
-
adjustMenu();
 
-
})
 
-
 
-
$(window).bind('resize orientationchange', function() {
 
-
ww = document.body.clientWidth;
 
-
adjustMenu();
 
-
});
 
-
 
-
var adjustMenu = function() {
 
-
if (ww < 768) {
 
-
$(".toggleMenu").css("display", "inline-block");
 
-
if (!$(".toggleMenu").hasClass("active")) {
 
-
$(".nav").hide();
 
-
} else {
 
-
$(".nav").show();
 
-
}
 
-
$(".nav li").unbind('mouseenter mouseleave');
 
-
$(".nav li a.parent").unbind('click').bind('click', function(e) {
 
-
// must be attached to anchor element to prevent bubbling
 
-
e.preventDefault();
 
-
$(this).parent("li").toggleClass("hover");
 
-
});
 
-
}
 
-
else if (ww >= 768) {
 
-
$(".toggleMenu").css("display", "none");
 
-
$(".nav").show();
 
-
$(".nav li").removeClass("hover");
 
-
$(".nav li a").unbind('click');
 
-
$(".nav li").unbind('mouseenter mouseleave').bind('mouseenter mouseleave', function() {
 
-
// must be attached to li so that mouseleave is not triggered when hover over submenu
 
-
$(this).toggleClass('hover');
 
-
});
 
-
}
 
-
}
 
-
  </script>
 
-
 
-
    <script type="text/javascript">
 
-
  WebFontConfig = {
 
-
    google: { families: [ 'PT+Sans::latin' ] }
 
-
  };
 
-
  (function() {
 
-
    var wf = document.createElement('script');
 
-
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 
-
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 
-
    wf.type = 'text/javascript';
 
-
    wf.async = 'true';
 
-
    var s = document.getElementsByTagName('script')[0];
 
-
    s.parentNode.insertBefore(wf, s);
 
-
  })();
 
-
</script>
 
-
 
-
</head>
 
-
 
-
<body>
 
<!-- TITLE -->
<!-- TITLE -->
Line 110: Line 12:
<div id="header" class="row-fluid">
<div id="header" class="row-fluid">
  <div class="span12">
  <div class="span12">
-
   <h3>E.coligy: Plants with BanAphids</h3>
+
   <h3><i>E. coligy</i>: Plants with BanAphids</h3>
  </div>
  </div>
</div>
</div>
Line 118: Line 20:
  <div class="span12 white">
  <div class="span12 white">
   <div class="row-fluid">
   <div class="row-fluid">
-
   <div class="span6">
+
   <div class="span8">
-
     <p align="justify"><b><a href="http://en.wikipedia.org/wiki/Aphid"> Aphids</a></b>, those pesky little green plant-sucking bugs, can pose serious threats to a farmer's proceeds. Not only is <b>physical damage</b> to the crops caused by the sucking a problem, but aphids also transmit harmful <b>viruses</b> to the plants. The magnitude of crop loss for the farmers is difficult to quantify as it varies with aphid species, crop species, location, year and many other factors, but it <b>can run in the millions</b>. Farmers try to control the aphid infestation with the use of <b>insecticides</b> but this is ineffective and contested. Aphids become resistant to insecticides quite rapid and insecticides also have a negative effect on their natural predators. That is why we, the <b><a href="http://www.kuleuven.be/kuleuven/"> KU Leuven </a> iGEM 2013 team</b>, decided to do something about this in a sustainable way by using an insecticide-free controlling mechanism. With <b>E.coligy: Plants with BanAphids</b> we will teach E.coli cells to hack into insects <b>signaling systems<b> to drive off the aphids and attract their <b>natural predators</b>, such as the ladybug</p>
+
     <p align="justify">
-
  </div>
+
The world population keeps on growing and since all people need to be fed while cultivable land is a limited resource, it is important to optimise crop yields. Different factors ranging from weather to diseases and pests have a huge impact on food supplies worldwide. An example of one of these factors are <b>aphid pests</b>. These can lead to <b>yield losses worth more than a quarter billion US dollars</b>! <br/>Aphids leech on plants everywhere: ranging from crop fields to fruit trees and even in vegetable and flower gardens. Farmers try to control them by using insecticides, but these harm the environment and aphids can become resistant to them quite readily. This is why we, the KU Leuven iGEM 2013 team, created an <b>ecological insecticide-free aphid controlling mechanism</b>, which mimics natural signalling systems used by both plants and insects. <br/>
-
   
+
-
  <div class="span6 greytext">
+
-
    <img src="https://static.igem.org/mediawiki/2013/0/09/KU_Leuven_team2.png" alt="Group picture"/>
+
   </div>
   </div>
 +
 +
    <div class="span4 video">
 +
      <p><iframe width="270" height="200" src="//www.youtube.com/embed/fS5mKMYnd6A?rel=0" frameborder="0" allowfullscreen></iframe></p>
 +
    </div>
 +
   </div>
   </div>
  </div>
  </div>
Line 135: Line 39:
<div class="row-fluid">
<div class="row-fluid">
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">   
 +
    <div class="visible-desktop span3 bg-purple">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/DataPage">
 +
    <i class="livicon activeicon" data-name="info" data-color="white"></i>
 +
    </div>
 +
  <div class="span7 icon-text">
 +
    <h3>Datapage</h3> </a>
 +
    <p>All our achievements on one small page!</p>
 +
  </div>
 +
  </div>
 +
</div>
  <div class="span4 icon white">
  <div class="span4 icon white">
Line 141: Line 58:
     <a href="https://2013.igem.org/Team:KU_Leuven/Project">
     <a href="https://2013.igem.org/Team:KU_Leuven/Project">
     <i class="livicon activeicon" data-name="bug" data-color="white"></i></div>
     <i class="livicon activeicon" data-name="bug" data-color="white"></i></div>
-
   <div class="span8 icon-text">
+
   <div class="span7 icon-text">
     <h3>Project</h3>
     <h3>Project</h3>
     </a>
     </a>
Line 154: Line 71:
     <a href="https://2013.igem.org/Team:KU_Leuven/Team">
     <a href="https://2013.igem.org/Team:KU_Leuven/Team">
     <i class="livicon activeicon" data-name="users" data-color="white"></i></div>
     <i class="livicon activeicon" data-name="users" data-color="white"></i></div>
-
   <div class="span8 icon-text">
+
   <div class="span7 icon-text">
     <h3>Team</h3> </a>
     <h3>Team</h3> </a>
     <p>Meet the team!</p>
     <p>Meet the team!</p>
Line 160: Line 77:
   </div>
   </div>
  </div>
  </div>
-
 
+
      
-
<div class="span4 icon white">
+
-
  <div class="row-fluid">
+
-
  <div class="visible-desktop span3 bg-yellow">
+
-
     <a href="https://2013.igem.org/Team:KU_Leuven/Notebook">
+
-
    <i class="livicon activeicon" data-name="notebook" data-color="white"></i>
+
-
    </div>
+
-
  <div class="span8 icon-text">
+
-
    <h3>Notebook</h3>
+
-
    </a>
+
-
    <p>What DID we do all summer?</p>
+
-
  </div>
+
-
  </div>
+
-
</div>    
+
   
   
</div>
</div>
-
<br/>
+
 
<div class="row-fluid">
<div class="row-fluid">
Line 187: Line 91:
     <i class="livicon activeicon" data-name="balance" data-color="white"></i>
     <i class="livicon activeicon" data-name="balance" data-color="white"></i>
   </div>
   </div>
-
   <div class="span8 icon-text">
+
   <div class="span7 icon-text">
     <h3>Human Practices</h3>
     <h3>Human Practices</h3>
     </a>
     </a>
-
     <p>Ethics, talks with industry and more.</p>
+
     <p>Ethics, talks with industry and more</p>
   </div>
   </div>
   </div>
   </div>
Line 198: Line 102:
   <div class="row-fluid">
   <div class="row-fluid">
   <div class="visible-desktop span3 bg-blue">
   <div class="visible-desktop span3 bg-blue">
-
   <a href="https://2013.igem.org/Team:KU_Leuven/Human_Outreach">
+
   <a href="https://2013.igem.org/wiki/index.php?title=Team:KU_Leuven/Project/modelling">
-
    <i class="livicon activeicon" data-name="sky-dish" data-color="white"></i></div>
+
  <i class="livicon activeicon" data-name="desktop" data-onparent="true" data-color="white"></i>    
-
   <div class="span8 icon-text">
+
  </div>
-
     <h3>Outreach</h3> </a>
+
   <div class="span7 icon-text">
-
     <p>Let the people know what we do.</p>
+
     <h3>Modelling</h3> </a>
 +
     <p>See our super computers in action!</p>
   </div>
   </div>
   </div>
   </div>
Line 208: Line 113:
  <div class="span4 icon white">
  <div class="span4 icon white">
-
   <div class="row-fluid">     
+
   <div class="row-fluid">
-
    <div class="visible-desktop span3 bg-purple">
+
   <div class="visible-desktop span3 bg-yellow">
-
     <a href="https://2013.igem.org/Team:KU_Leuven/Sponsors">
+
     <a href="https://2013.igem.org/Team:KU_Leuven/Notebook">
-
     <i class="livicon activeicon" data-name="piggybank" data-color="white"></i>
+
     <i class="livicon activeicon" data-name="notebook" data-color="white"></i>
     </div>
     </div>
-
   <div class="span8 icon-text">
+
   <div class="span7 icon-text">
-
     <h3>Sponsors</h3> </a>
+
     <h3>Notebook</h3>
-
     <p>Meet our most generous sponsors!</p>
+
    </a>
 +
     <p>What DID we do all summer?</p>
   </div>
   </div>
   </div>
   </div>
-
  </div>
+
  </div>
</div>
</div>
-
<br/>
 
-
<br/>
+
<!-- TITLE -->
 +
<div id="header" class="row-fluid">
 +
<div class="span12">
 +
  <a href="https://2013.igem.org/Team:KU_Leuven/Project"> 
 +
  <h3 class="bg-green">The Project</h3> </a>
 +
</div>
 +
</div>
 +
 +
 +
<div class="row-fluid">
 +
<div class="span12 white">
 +
  <div class="row-fluid">
 +
  <div class="span8">
 +
  <p align="justify">
 +
Our bacteria are called <b>BanAphids and produce two pheromones</b>. The first one is methyl salicylate, which plays a role in a <a href="https://2013.igem.org/Team:KU_Leuven/Project/Ecological/Background#MeS effect on plants1">plant’s defence response</a> and is produced by the plant upon aphid infestation. The second pheromone is E-β-farnesene, which is an aphid alarm pheromone. In our system the BanAphids produce E-β-farnesene <b>to repel aphids</b>, while the methyl salicylate is meant <b>to attract their natural predators</b> such as ladybugs. In order to provide a proof of principle we contacted <b> companies specialised in crop protection</b>, <a href="http://www.biobest.be">Biobest</a> and <a href="http://www.pcfruit.be">pc fruit</a>, where we showed the functionality of these pheromones by performing insect experiments. Both companies were very interested and saw our project as a promising alternative solution.<br/>
 +
 +
We designed <b>two systems</b> of implementation for our BanAphids. In the <a href="https://2013.igem.org/Team:KU_Leuven/Project/HoneydewSystem">first</a> system, BanAphids are <b>sprayed on the plants</b> and in this case the production of pheromones is dependent on the presence of aphids. This system avoids the habituation of aphids to E-β-farnesene by only becoming active in the presence of aphids. The <a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem">second</a> system consists of <b>a sticker containing BanAphids</b>, that can be attached to a plant. This sticker system alternates the production of the pheromones by the use of <b>our own designed oscillating system</b>. This <a href="https://2013.igem.org/Team:KU_Leuven/Project/Oscillator/Description">oscillator</a> was extensively modelled by our team.</p>
 +
  </div>
 +
  <div class="span4 greytext video">
 +
  <img src="https://static.igem.org/mediawiki/2013/0/07/Tekeningproject.jpg" alt="banaphids">
 +
 +
  <img src="https://static.igem.org/mediawiki/2013/5/5d/Stickermodelverkleind.png">
 +
  </div>
 +
 +
 +
  </div>
 +
</div>
 +
</div>
<!--Video -->
<!--Video -->
Line 232: Line 164:
  <div class="span12 icon-half">
  <div class="span12 icon-half">
   <div class="row-fluid">
   <div class="row-fluid">
-
   <div class="span6 white">
+
 
 +
   <div class="span12 white">
     <div class="row-fluid">
     <div class="row-fluid">
-
     <div class="span3 bg-red visible-desktop">
+
 
 +
     <div class="span3 bg-green visible-desktop">
     <i class="livicon activeicon" data-name="youtube" data-onparent="true" data-color="white"></i>
     <i class="livicon activeicon" data-name="youtube" data-onparent="true" data-color="white"></i>
     </div>
     </div>
-
     <div class="span9 video">
+
     <div class="span10 video">
-
       <h3>Video</h3>
+
       <h3>Pilot study!</h3>
-
       <p><iframe width="340" height="255" src="//www.youtube-nocookie.com/embed/x2OigoNwElk?rel=0" frameborder="0" allowfullscreen></iframe></p>
+
<br/>
 +
<br/>
 +
       <p><iframe width="740" height="455" src="//www.youtube.com/embed/n-pMp8-XdUo?rel=0" frameborder="0" allowfullscreen></iframe></p>
 +
      <p align="justify">In this video you will see the first behavioural experiment with aphids and BanAphids. An EBF-producing bacterium plate is placed on the left, a blank on the right, aphids are placed on the leaf in the middle. The first results seem positive, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.)</p>
     </div>
     </div>
 +
     </div>
     </div>
   </div>
   </div>
 +
   </div>
   </div>
  </div>
  </div>
</div>
</div>
-
</body>
 
-
</html>
 
-
<!-- Sources:
+
<!-- TITLE -->
-
http://genomebiology.com/2006/7/3/r21#B2
+
-
-->
+
-
Like us on [http://www.facebook.com/pages/KU-Leuven-iGEM/226385510728487 facebook] <br/>
+
<div id="header" class="row-fluid">
-
Follow us on [http://twitter.com/KULeuven_iGEM twitter] <br/>
+
<div class="span12">
-
See us on [http://www.youtube.com/user/KULeuveniGEM youtube] <br/>
+
  <a href="https://2013.igem.org/Team:KU_Leuven/Human_Practices">
 +
  <h3 class="bg-red">Human Practices</h3></a>
 +
</div>
 +
</div>
 +
<div class="row-fluid">
 +
<div class="span12 white">
 +
  <div class="row-fluid">
 +
  <div class="span3 greytext">
 +
  <br/>
 +
    <img src="https://static.igem.org/mediawiki/2013/c/c0/Debat_Leuven_speakers.jpg">
 +
    <p align="justify">Speakers and the moderator at our debate.</p>
 +
   
 +
    <img src="https://static.igem.org/mediawiki/2013/1/10/Plexiglas_models.JPG"/>
 +
    <p aling="justify">We designed our own BioBricks for the educational workshops!</p>
 +
  </div>
 +
 +
  <div class="span9">
 +
  <p align="justify">
 +
Since <b>BanAphids</b> will be introduced in the environment, the ethical aspect of our project cannot be underestimated. Therefore we developed a new additional approach: <b>Bottom-up or ethics from within!</b>
 +
In order to improve communication between different stakeholders and achieve reciprocal understanding in early stages of the project, we decided to develop a new additional approach from which the government, scientists, the public and even the industry can benefit.<br/>
 +
This new approach is bottom-up structured where the dialogue between philosophers, scientists and the general public is central. And with the help of a unique collaboration between a <b>philosophy student who joined the KU Leuven iGEM team</b> and the general public, we have developed a new and strong <a href="https://2013.igem.org/Team:KU_Leuven/Human_Practices/Bottom_Up_Philosopher">framework</a> for this approach. <br/><br/>
 +
 +
We organised <b>a <a href="https://2013.igem.org/Team:KU_Leuven/Human_Outreach/Seminars">symposium</a> and a <a href="https://2013.igem.org/Team:KU_Leuven/Human_Practices/Ethics/Debate">debate</a></b> to inform the general public about our project and to open the discussion on the ethical aspects. We invited our neighbouring Benelux (Belgium, The Netherlands, Luxembourgh) iGEM teams for both events. Some teams participated this year so we are now considering a "Benelux-GEM slam" for the future. We also went out on the street and interviewed <a href="https://2013.igem.org/Team:KU_Leuven/Human_Practices/EndUser">farmers</a> to ask if they would use <b>BanAphids</b> and visited several <a href="https://2013.igem.org/Team:KU_Leuven/Human_Outreach/Education">schools</a> with our own <b>BioBricks</b> and 3D-bacterium design to let students take their first steps in the fascinating world of synthetic biology.</p>
 +
  </div>
 +
 +
  </div>
 +
</div>
 +
</div>
 +
 +
 +
 +
<!--Picture gallery -->
 +
 +
<div class="row-fluid">
 +
<div class="span12 icon-half">
 +
  <div class="row-fluid">
 +
  <div class="span12 white">
 +
    <div class="row-fluid">
 +
 +
    <div class="span3 bg-maroon visible-desktop">
 +
      <i class="livicon activeicon" data-name="flickr" data-onparent="true" data-color="white"></i>
 +
    </div>
 +
 +
    <div class="span10 icon-text ">
 +
      <h3>Picture gallery</h3>
 +
      <div id="banner-fade">
 +
      <ul class="bjqs">
 +
        <li><img src="https://static.igem.org/mediawiki/2013/5/5c/Farmer%27s_inquiry.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/d/d8/Whatisit.jpg" title="Automatically generated caption"></li>
 +
        <li><img src ="https://static.igem.org/mediawiki/2013/e/ef/Bbq1.JPG" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/2/23/2013-07-01_1334.34.41.jpg" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/f/f8/Bbq2.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/1/1c/2013-08-07_11.27.46.jpg" title= "Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/5/53/Biobestplants.jpg" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/4/4b/Calgary11.jpg" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/f/f4/Lad22013-07-01_14.39.54-2.jpg" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/f/f3/Sander1.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/8/89/EosCollage11.jpg" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/9/9d/Wet1.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/6/6f/Photo2.JPG" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/3/32/Bbq4.JPG" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/5/59/Wet2.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/2/25/Wet3.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/f/f8/Lad1.jpg" title="Automatically generated caption"</li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/9/98/Pcf1.JPG" title="Automatically generated caption"></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2013/7/77/Pcf2.JPG" title="Automatically generated caption"</li>
 +
 +
      </ul>
 +
    </div>
 +
    </div>
 +
  </div>
 +
  </div>
 +
 +
  </div>
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
</body>
 +
</html>
{{:Team:KU Leuven/Template:Sponsors}}
{{:Team:KU Leuven/Template:Sponsors}}

Latest revision as of 01:24, 29 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

The world population keeps on growing and since all people need to be fed while cultivable land is a limited resource, it is important to optimise crop yields. Different factors ranging from weather to diseases and pests have a huge impact on food supplies worldwide. An example of one of these factors are aphid pests. These can lead to yield losses worth more than a quarter billion US dollars!
Aphids leech on plants everywhere: ranging from crop fields to fruit trees and even in vegetable and flower gardens. Farmers try to control them by using insecticides, but these harm the environment and aphids can become resistant to them quite readily. This is why we, the KU Leuven iGEM 2013 team, created an ecological insecticide-free aphid controlling mechanism, which mimics natural signalling systems used by both plants and insects.


Datapage

All our achievements on one small page!

Project

Want to know more about our project?

Team

Meet the team!

Human Practices

Ethics, talks with industry and more

Modelling

See our super computers in action!

Notebook

What DID we do all summer?

Our bacteria are called BanAphids and produce two pheromones. The first one is methyl salicylate, which plays a role in a plant’s defence response and is produced by the plant upon aphid infestation. The second pheromone is E-β-farnesene, which is an aphid alarm pheromone. In our system the BanAphids produce E-β-farnesene to repel aphids, while the methyl salicylate is meant to attract their natural predators such as ladybugs. In order to provide a proof of principle we contacted companies specialised in crop protection, Biobest and pc fruit, where we showed the functionality of these pheromones by performing insect experiments. Both companies were very interested and saw our project as a promising alternative solution.
We designed two systems of implementation for our BanAphids. In the first system, BanAphids are sprayed on the plants and in this case the production of pheromones is dependent on the presence of aphids. This system avoids the habituation of aphids to E-β-farnesene by only becoming active in the presence of aphids. The second system consists of a sticker containing BanAphids, that can be attached to a plant. This sticker system alternates the production of the pheromones by the use of our own designed oscillating system. This oscillator was extensively modelled by our team.

banaphids

Pilot study!



In this video you will see the first behavioural experiment with aphids and BanAphids. An EBF-producing bacterium plate is placed on the left, a blank on the right, aphids are placed on the leaf in the middle. The first results seem positive, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.)


Speakers and the moderator at our debate.

We designed our own BioBricks for the educational workshops!

Since BanAphids will be introduced in the environment, the ethical aspect of our project cannot be underestimated. Therefore we developed a new additional approach: Bottom-up or ethics from within! In order to improve communication between different stakeholders and achieve reciprocal understanding in early stages of the project, we decided to develop a new additional approach from which the government, scientists, the public and even the industry can benefit.
This new approach is bottom-up structured where the dialogue between philosophers, scientists and the general public is central. And with the help of a unique collaboration between a philosophy student who joined the KU Leuven iGEM team and the general public, we have developed a new and strong framework for this approach.

We organised a symposium and a debate to inform the general public about our project and to open the discussion on the ethical aspects. We invited our neighbouring Benelux (Belgium, The Netherlands, Luxembourgh) iGEM teams for both events. Some teams participated this year so we are now considering a "Benelux-GEM slam" for the future. We also went out on the street and interviewed farmers to ask if they would use BanAphids and visited several schools with our own BioBricks and 3D-bacterium design to let students take their first steps in the fascinating world of synthetic biology.

Picture gallery