"

From 2013.igem.org

Revision as of 17:37, 8 September 2013 (view source) Tore (Talk | contribs) ← Older edit		Revision as of 11:09, 15 September 2013 (view source) ManuelS (Talk | contribs) Newer edit →
Line 15:		Line 15:
	<br>		<br>
-	The Deletion of the Alanine-Racemase in ''Escherichia coli'' leeds to a strict auxotrophic mutant. D-Alanin is an essential	+	We produced a auxotrophic safety strain which was the basic for our biosafety system. The strain consists Δdadx and Δalr knockouts which prevents the expression of alanine racemase. Alanine racemase is an isomerase that catalyzes the chemical reaction from L-alanine to D-alanine. D-alanine appears in peptidoglycan which is an important component of the bacterial cell wall. So the safety strain depends on the supplementation of alanine racemase on the plasmid which causes a good plasmid stability. If the bacteria cast the plasmid it will die because of the fact that L-alanine couldn’t be isomerized into D-alanine anymore and the cell wall wouldn’t be assemble in the correct way.

---

Revision as of 11:09, 15 September 2013

Biosafety-Strain

We produced a auxotrophic safety strain which was the basic for our biosafety system. The strain consists Δdadx and Δalr knockouts which prevents the expression of alanine racemase. Alanine racemase is an isomerase that catalyzes the chemical reaction from L-alanine to D-alanine. D-alanine appears in peptidoglycan which is an important component of the bacterial cell wall. So the safety strain depends on the supplementation of alanine racemase on the plasmid which causes a good plasmid stability. If the bacteria cast the plasmid it will die because of the fact that L-alanine couldn’t be isomerized into D-alanine anymore and the cell wall wouldn’t be assemble in the correct way.