Team:UNITN-Trento/Notebook/Labposts/07/04

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(Difference between revisions)
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{
{
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"date" : "2013-07-19",
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"date" : "2013-07-02",
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"author" : "emil-viola",
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"author" : "caterina-michele",
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"title" : "The incompetent Bacillus subtilis saga OD(io)",
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"title" : "Incredible: E.coli could growth!",
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"content" : "<html>We have tied to grow B.subtilis in the minimal medium (Cambridge protocol and Mansy's friend's protocol) with no evidence of growth, in the previous days we have observed that B. subtilis grow in LB so we have tried to transform B. subtilis in  LB following the LMU munich protocol modified.We transformed 2 groups of cells with the construct K823026+GFP but we mistaken the right Kanamicin concentration of the stock solution(10mg/ml)so no the following day ther were no colonies on the plates.</html>",
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"content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ",
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"tags" : "Bacillus subtilis"
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"tags" : "PchBA-BMST1-araC-pBAD"
}
}

Revision as of 08:21, 3 October 2013

{ "date" : "2013-07-02", "author" : "caterina-michele", "title" : "Incredible: E.coli could growth!", "content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ", "tags" : "PchBA-BMST1-araC-pBAD" }

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