Team:UNITN-Trento/Notebook/Labposts/06/24

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-14",
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"date" : "2013-06-13",
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"author" : "caterina",
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"author" : "Emil",
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"title" : "Digestion& ligation of BMST1 (BBa_J45119) and PCHA (BBa_J45319)",
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"title" : "Verfying the three backbone",
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"content" : "<html>I digested the pSB1C3 linearized with EcoR I and Spe I. <br/>Next I perfomed six different ligations:<br/>two controls with: the pSB1C3 linearized and not but without the insert<br/>two  with: pSB1C3 linearized or not and BMST1<br/>two with: pSB1C3 linearized or not and PCHA</html>",
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"content" : "<html>We have verifyed the parts from LMU Munich doing an electrophoresis after having digested the parts with Pst1 and EcoR1.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Results|<html><center><table class=\"tn-sp-table\"><tr><th>Well</th><th colspan=\"10\">Loaded samples</th></tr><tr><td>1</td><td>DNA Ladder 1Kb</td></tr><tr><td>2</td><td>#1BBa_J823022(A)</td></tr><tr><td>3</td><td>#2BBa_J823023(A)</td></tr><tr><td>4</td><td>#4BBa_J823027(A)</td></tr><tr><td>5</td><td>#5BBa_J823022(B)</td></tr><tr><td>6</td><td>#6BBa_J823023(B)</td></tr><tr><td>7</td><td>#7Bba_J823027(B)</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/b/be/Tn-20130613-Gel_LMU_Munich.jpg\" width=\"400px\" /></center></html>}}<html></html>",
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"tags" : "BSMT1-PchA"
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"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
}
}

Latest revision as of 07:45, 3 October 2013

{ "date" : "2013-06-13", "author" : "Emil", "title" : "Verfying the three backbone", "content" : "We have verifyed the parts from LMU Munich doing an electrophoresis after having digested the parts with Pst1 and EcoR1.

Gel Results
WellLoaded samples
1DNA Ladder 1Kb
2#1BBa_J823022(A)
3#2BBa_J823023(A)
4#4BBa_J823027(A)
5#5BBa_J823022(B)
6#6BBa_J823023(B)
7#7Bba_J823027(B)
", "tags" : "pSBBs1C-pSBBs4S-pSBBs2E" }

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