Team:UNITN-Trento/Notebook/Labposts/06/44
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{ | { | ||
- | "date" : "2013-06- | + | "date" : "2013-06-20", |
- | "author" : " | + | "author" : "emil-gabriele", |
- | "title" : " | + | "title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase", |
- | "content" : "<html> | + | "content" : "<html>We quantified yesterday inocula (1:1, 1:2, 1:4 of SAMsynthetase and R0010, both in pSB1C3).</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantification (ng/µl)</th></tr><tr><td>1:1 A</td><td>293.0</td></tr><tr><td>1:1 B</td><td>135.7</td></tr><tr><td>1:1 C</td><td>178.0</td></tr><tr><td>1:1 D</td><td>139.3</td></tr><tr><td>1:1 E</td><td>262.0</td></tr><tr><td>1:1 F</td><td>147.5</td></tr><tr><td>1:1 G</td><td>212.5</td></tr><tr><td>1:1 H</td><td>210.2</td></tr><tr><td>1:2 A</td><td>141.0</td></tr><tr><td>1:2 B</td><td>123.6</td></tr><tr><td>1:4 A</td><td>207.8</td></tr><tr><td>1:4 B</td><td>127.2</td></tr><tr><td>R0010 A</td><td>131.7</td></tr><tr><td>R0010 B</td><td>119.6</td></tr><tr><td>R0010 C</td><td>371.0</td></tr><tr><td>R0010 D</td><td>307.9</td></tr><tr><td>R0010 E</td><td>313.8</td></tr></table></center></html>}}<html>Then, we screened the products of ligation (1:1, 1:2, 1:4 and R0010): digestion with EcoRI-HF and PstI-HF following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol</a> and agarose gel 1%.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading Scheme</th></tr><tr><td><i>Empty</i></td></tr><tr><td>1kb ladder</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:1 D</td></tr><tr><td>1:1 E</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:2 B</td></tr><tr><td><i>Empty</i></td></tr><tr><td>1:4 A</td></tr><tr><td><i>Empty</i></td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/5/54/Tn-20130620-ET_GG_screening_SAM_R0010_Failed.jpg\" width=\"450px\" /> <!-- Sams primo screening--></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>1kb ladder</td></tr><tr><td>R0010 A</td></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/1/1d/Tn-20130620-ET_screening_psB1C3SAM_FAILED.jpg\" width=\"450px\" /> <!-- Sams 1:1 and PCR(last 3) --></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>1kb ladder</td></tr><tr><td>1:4 A</td></tr><tr><td>1:4 B</td></tr><tr><td>1:2 A</td></tr><tr><td>1:2 B</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/a/aa/Tn-20130620-GG_R0010_pSB1C3SAM_FAILED.jpg\" width=\"450px\" /> <!-- R0010 --></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|1.5% Agarose Gel Image|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td>1kb ladder</td></tr><tr><td>R0010 A</td></tr><tr><td>R0010 B</td></tr><tr><td>R0010 C</td></tr><tr><td>R0010 D</td></tr><tr><td>R0010 E</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/8/8b/Tn-20130620-GG_R0010_screening_FAILED.jpg\" width=\"450px\" /> <!-- R0010 bis --></center></html>}}<html>In these images we can only see the band of the backbone and no insert!</html>", |
- | "tags" : " | + | "tags" : "SAMsynthetase" |
} | } |
Latest revision as of 07:52, 3 October 2013
{ "date" : "2013-06-20", "author" : "emil-gabriele", "title" : "Linearization of psB1C3(Failed) and screening of SAM sinthethase", "content" : "We quantified yesterday inocula (1:1, 1:2, 1:4 of SAMsynthetase and R0010, both in pSB1C3).
Quantification results
Then, we screened the products of ligation (1:1, 1:2, 1:4 and R0010): digestion with EcoRI-HF and PstI-HF following the digestion protocol and agarose gel 1%.Sample | Quantification (ng/µl) |
---|---|
1:1 A | 293.0 |
1:1 B | 135.7 |
1:1 C | 178.0 |
1:1 D | 139.3 |
1:1 E | 262.0 |
1:1 F | 147.5 |
1:1 G | 212.5 |
1:1 H | 210.2 |
1:2 A | 141.0 |
1:2 B | 123.6 |
1:4 A | 207.8 |
1:4 B | 127.2 |
R0010 A | 131.7 |
R0010 B | 119.6 |
R0010 C | 371.0 |
R0010 D | 307.9 |
R0010 E | 313.8 |
1% Agarose Gel Image
Loading Scheme |
---|
Empty |
1kb ladder |
Empty |
1:1 D |
1:1 E |
Empty |
1:2 B |
Empty |
1:4 A |
Empty |
R0010 E |
1% Agarose Gel Image
Loading scheme |
---|
1kb ladder |
R0010 A |
R0010 B |
R0010 C |
R0010 D |
R0010 E |
1% Agarose Gel Image
Loading scheme |
---|
R0010 B |
R0010 C |
R0010 D |
1kb ladder |
1:4 A |
1:4 B |
1:2 A |
1:2 B |
1.5% Agarose Gel Image
In these images we can only see the band of the backbone and no insert!",
"tags" : "SAMsynthetase"
}
Loading scheme |
---|
1kb ladder |
R0010 A |
R0010 B |
R0010 C |
R0010 D |
R0010 E |