Team:UNITN-Trento/Notebook/Labposts/06/52

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(Difference between revisions)

Latest revision as of 07:56, 3 October 2013

{ "date" : "2013-06-24", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters and plasmids (part 7)!!", "content" : " we realized that the other day we put the wrong antibiotic in the new plasmids inocula; according to that Bruno found out that nothing grew in there. So this afternoon we made inocula again, this time with Ampicilline.We also made some inocula of the 3 confermed promoters just to have enough stock material.", "tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac " }

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 {
-	"date" : "2013-06-20",
+	"date" : "2013-06-24",
-	"author" : "emil",
+	"author" : "fabio-bruno",
-	"title" : "Linearization of psB1C3 (Failed)",
+	"title" : "bacillus subtilis promoters and  plasmids (part 7)!!",
-	"content" : "<html>I have re-tried to linearize through a PCR the backbone PsB1C3 that we have previously purified and quntified.<br>I have tried to amplify the sample of 117.3 ng/&micro;l, 105.7 ng/&micro;l and  one of Girelli's sample(184.1 ng/&micro;l)following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols\">Phusion PCR protocol</a> and the universal primers.<br>No one of this PCRs has succeded!   </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Linearization attempt 2|<html><center>width=\"450px\" /></html>}}<html></html>",
+	"content" : "<html> we realized that the other day we put the wrong antibiotic in the new plasmids inocula; according to that Bruno found out that nothing grew in there. So this afternoon we made inocula again, this time with Ampicilline.We also made some inocula of the 3 confermed promoters just to have enough stock material.</html>",
-	"tags" : "pSB1C3"
+	"tags" : " Pveg-PlepA-plasmidPxil-plasmidPspac "
 }