From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-07-02", | + | "date" : "2013-07-19", |
| - | "author" : "fabio-viola", | + | "author" : "fabio-bruno", |
| - | "title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!", | + | "title" : " blue and red light sensors!!", |
| - | "content" : "<html> in order to propagate B. subtilis from the pellet that we purchased (strain ind- tyr+ B.subtilis ATCC 23857) , first we needed to obtain the perfect medium for its growth. We prepared Nutrient Broth for liquid cultures and Nutrient Broth + Agar for plates, following ATCC PROTOCOLS. So we decided to have them in both a STARCH-added version and a version without starch.<br>Nutrient agar (100 ml) : 0,8 g nutrient broth: 1,5g Agar; water to 100 ml.<br>Nutrient agar + starch (100 ml) : 0,8 g nutrient broth: 1,5g Agar; 2ml starch from potato, water up to 100 ml.<br>Nutrient broth (250 ml): 2 g nutrient broth; 250 ml water.<br>Nutrient broth + starch (250 ml): 2 g nutrient broth; 5 ml starch; water up to 250 ml; <br>To propagate di original bacillus pellet, we resuspended it in 1 ml of nutrient broth+starch and put this milliliter in 5 ml of nutrient broth+starch for a final 6 ml mother liquid culture. Then we made several other liquid cultures with different bacteria concentrations from the Mother (for each concentration we made both starch and non-starch liquid cultures). We put all in a shaker at 26 degrees. We plated also the same concentrations in several plates with both Nutrient agar + starch and Nutrient agar alone, and put them at 26. Just out of curiosity we decided to put some plates and some liquid cultures at 37 degrees.</html>", | + | "content" : "<html> today we tried to pcr yesterday’s minipreps out with Taq polymerase but even this time the screening wasn’t successful at all!! <br>We inoculated for the THIRD time the 4 parts too!</html>", |
| - | "tags" : " Bacillus subtilis " | + | "tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR" |
| | } | | } |
Revision as of 08:34, 3 October 2013
{
"date" : "2013-07-19",
"author" : "fabio-bruno",
"title" : " blue and red light sensors!!",
"content" : " today we tried to pcr yesterday’s minipreps out with Taq polymerase but even this time the screening wasn’t successful at all!!
We inoculated for the THIRD time the 4 parts too!",
"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
}
"
