Team:UNITN-Trento/Notebook/Labposts/07/38

From 2013.igem.org

(Difference between revisions)

Revision as of 08:35, 3 October 2013

{ "date" : "2013-07-19", "author" : "emil-viola", "title" : "The incompetent Bacillus subtilis saga OD(io)", "content" : "We have tied to grow B.subtilis in the minimal medium (Cambridge protocol and Mansy's friend's protocol) with no evidence of growth, in the previous days we have observed that B. subtilis grow in LB so we have tried to transform B. subtilis in LB following the LMU munich protocol modified.We transformed 2 groups of cells with the construct K823026+GFP but we mistaken the right Kanamicin concentration of the stock solution(10mg/ml)so no the following day ther were no colonies on the plates.", "tags" : "Bacillus subtilis" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-03",
+	"date" : "2013-07-19",
-	"author" : "fabio-viola",
+	"author" : "emil-viola",
-	"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
+	"title" : "The incompetent Bacillus subtilis saga OD(io)",
-	"content" : "<html> HORROR!! We lost kind of the whole bacillus that we had, during the night, cause the liquid cultures were disrupted in the shaker at 26!! Luckily we have a survivor, the liquid culture at 37, and all the plates. <br>From the survivor we made further liquid cultures ( at 26°, 1:50 and 1:100; at 37° 1:50 and 1:100) <br>As soon as they became very cloudy we went along with the glycerol stock preparation and put our bacillus at -80°. The one that weren’t cloudy have been kept in the shaker all night long. <br>In the afternoon we inoculated some colonies from the plates in 5 ml of nutrient broth +starch.</html>",
+	"content" : "<html>We have tied to grow B.subtilis in the minimal medium (Cambridge protocol and Mansy's friend's protocol) with no evidence of growth, in the previous days we have observed that B. subtilis grow in LB so we have tried to transform B. subtilis in  LB following the LMU munich protocol modified.We transformed 2 groups of cells with the construct K823026+GFP but we mistaken the right Kanamicin concentration of the stock solution(10mg/ml)so no the following day ther were no colonies on the plates.</html>",
-	"tags" : " Bacillus subtilis"
+	"tags" : "Bacillus subtilis"
 }