From 2013.igem.org
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| | { | | { |
| - | "date" : "2013-07-03", | + | "date" : "2013-07-21", |
| - | "author" : "emil", | + | "author" : "fabio-bruno", |
| - | "title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ", | + | "title" : " blue and red light sensors!!", |
| - | "content" : "<html> I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\"> transformation protocol</a>.These are the results of the plate:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:1 |<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:1|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 control|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 control|<html> <img src=\"\" /></html>}}<html>Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).</html>", | + | "content" : "<html> today we decided to get our parts sequenced, so I prepared sequencing samples and tomorrow they will be sent to a sequencing company. <br>We also inoculate the new part: k952003. From now on Bruno and I will take our own paths!!</html>", |
| - | "tags" : "K832024-K823026-E0840" | + | "tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR-YF1_FixJ_PfixK2_amilGFP" |
| | } | | } |
Revision as of 08:36, 3 October 2013
{
"date" : "2013-07-21",
"author" : "fabio-bruno",
"title" : " blue and red light sensors!!",
"content" : " today we decided to get our parts sequenced, so I prepared sequencing samples and tomorrow they will be sent to a sequencing company.
We also inoculate the new part: k952003. From now on Bruno and I will take our own paths!!",
"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR-YF1_FixJ_PfixK2_amilGFP"
}
"
