Team:UNITN-Trento/Notebook/Labposts/07/64

From 2013.igem.org

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{
{
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"date" : "2013-07-19",
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"date" : "2013-07-30",
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"author" : "caterina",
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"author" : "fabio",
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"title" : "SAM sythetase and other miniprep",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html>Yesterday, I performed inocula from different plates prepared by Gire. After miniprep and screening we had the confirmation that into pSB1C3 there was RFP and not SAM synthetase. So on Monday we will repeat the experiment.<br/><br/>Then I performed the miniprep of some inocula that I prepared the yesterday containing K1065101 and K1065102.</html>",
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"content" : "<html> today unfortunately I miniprepped the last inocula (from ligation nr. 4) and screening didn’t verify the presence of any ligation. What a drag!! The only thing that remains to be done is to inoculate more and more colonies from previous plates: 6 from ligation 3 (plate 1:1) and 6 from ligation nr. 4 (plate 1:1).</html>",
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"tags" : "SAM-K1065101-K1065102"
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"tags" : " YF1_FixJ - FixK2"
}
}

Revision as of 08:46, 3 October 2013

{ "date" : "2013-07-30", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : " today unfortunately I miniprepped the last inocula (from ligation nr. 4) and screening didn’t verify the presence of any ligation. What a drag!! The only thing that remains to be done is to inoculate more and more colonies from previous plates: 6 from ligation 3 (plate 1:1) and 6 from ligation nr. 4 (plate 1:1).", "tags" : " YF1_FixJ - FixK2" }