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09/09/13
From 2013.igem.org
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| + | ==Miniprep of overnight broth== | ||
* Following on from [[06/09/13]] result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on [[06/09/13]]. | * Following on from [[06/09/13]] result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on [[06/09/13]]. | ||
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* The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit. | * The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit. | ||
* This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product. | * This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product. | ||
Revision as of 12:17, 9 September 2013
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Miniprep of overnight broth
- Following on from 06/09/13 result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on 06/09/13.
- The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit.
- This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product.
Measurement of DNA concentration
Gel electrophoresis of isolated plasmids
- The plasmids that were isolated from the miniprep were run on a 1% agarose gel to confirm distinguish between cells that have the pSB1C3/TOD genes from those that have the recircularised pSB1C3 vector.
