Team:Groningen/Labwork/9 September 2013

From 2013.igem.org

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<br>S3 and S9 were digested with BamHI and PstI.
<br>S3 and S9 were digested with BamHI and PstI.
<br>S16 was digested with EcoRI and BamHI.
<br>S16 was digested with EcoRI and BamHI.
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<br>S16 was ligated to S3 and S9.
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<br>S16 was ligated to both S3 and S9.
<br>The ligation reaction was run on gel 0.8% to check the presence of product.
<br>The ligation reaction was run on gel 0.8% to check the presence of product.
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<br>

Revision as of 13:33, 9 September 2013

Claudio

S13 and S14 were digested with XbaI and PstI and purified.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were digested with SpeI and PstI.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were ligated to S13 and S14, respectively.
The ligation product was transformed in E. Coli DH5α and plated on LB + Cm.

S3 and S9 were digested with BamHI and PstI.
S16 was digested with EcoRI and BamHI.
S16 was ligated to both S3 and S9.
The ligation reaction was run on gel 0.8% to check the presence of product.

Sebas