Team:BIT/Team

From 2013.igem.org

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<p><img src="https://static.igem.org/mediawiki/2013/7/7a/BITflag.gif" width="1024" height="150" border="0" usemap="#Map">
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<p><a href="https://2013.igem.org/Team:BIT/project#bio"><img src="https://static.igem.org/mediawiki/2013/1/16/BITgreenA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#amp"><img src="https://static.igem.org/mediawiki/2013/2/2c/BITpurpleA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#chip"><img src="https://static.igem.org/mediawiki/2013/7/76/BITyellowA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#dev"><img src="https://static.igem.org/mediawiki/2013/f/fa/BITblueA.gif" width="240" height="320" /></a></p>
<p><a href="https://2013.igem.org/Team:BIT/project#bio"><img src="https://static.igem.org/mediawiki/2013/1/16/BITgreenA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#amp"><img src="https://static.igem.org/mediawiki/2013/2/2c/BITpurpleA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#chip"><img src="https://static.igem.org/mediawiki/2013/7/76/BITyellowA.gif" width="240" height="320" /></a><a href="https://2013.igem.org/Team:BIT/project#dev"><img src="https://static.igem.org/mediawiki/2013/f/fa/BITblueA.gif" width="240" height="320" /></a></p>
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<a href="2013.igem.org/Team:BIT">Home</a>
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<a href="#Mem">Team Members
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<p><span class="Tit"> <span class="White"><span class="White"><br /><br />
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    <td colspan="2" class="Cap"><a name="Mem"></a>Team Members</td>
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     <td width="730"><p class="Cap1">A Detailed Introduction of Integrated Sensor</p>
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      <p class="Cap1"> <a name="top"></a>for Detection of Milk Product</p></td>
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    <td width="471"><p>Xin Ma is the president of this team in 2013,  who has been majoring in Biomedical Engineering in Beijing Institute of  Technology since 2011. Debate, speech, board games, writing and composing are  his five favorites. He would like to take advantage of the new discovery  related to human health to create a better and more beautiful life for all the  mankind. For him, iGEM provides an excellent platform to take a step forward to  achieve his ideal;</p>
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      <p>&nbsp;</p></td>
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     <td><p class="Cap">Biosensors</p>
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      <p><a name="bio"></a></p>
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    <td>an undergraduate student who has been majoring in biotechnology since 2011.She is a girl who is very outgoing and passionate .In her spare time, she loves reading books, listening to music and dancing. She is the vice-captain of the group. She takes part in the competition to creatively study and apply the relevant knowledge. She is in charge of the biological experiment and deal with some affairs of the group. She believes science and technology can benefit mankind and wants to contribute to the development of mankind.</td>
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      <p><span class="Cap">Beta-lactam detection device</span><br>
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      <p>Background<br>
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    <td><img src="https://static.igem.org/mediawiki/2013/5/5d/%E5%BC%A0%E8%A1%8C%E5%81%A5.jpg" width="97" height="97"></td>
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        In order to prevent cow mastitis, all the producers of diary products feed the cows with antibiotics. However, excessive residual antibiotics will increase the drug resistance on human body. According to international standards for antibiotics, most dairy farmers use beta-lactams, such as penicillin deviants and cephalosporin which exceed quality standards on their cows. The beta-lactam biosensor is designed for the detection of beta-lactam in dairy products.</p>
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    <td><p> Biomedical Engineering juniorundergraduates, head of microfluidic chip group, also the contact person of BIT-IGEM team, whose mottois&quot;Life is acontinuous improvementjourney&quot;.Lovemusic and sports. Band drummer and also a football team striker. Taking part in this competition in order to improve personal ability by learning from other’s advantage and gain more experience after enjoy the time of this competition. Expecting for this communication and looking forward to meet with every kind of friends in different culture background.</p>
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      <p>Beta-lactam biosensor is aimed to create a biosensor that can be applied in  practical life. It is useful for citizens to know what they drink and what they buy for their little babies are qualified and hygienic. While there are traditional methods to detect beta-lactam antibiotics, such as enzyme-linked immunosorbent assay (ELISA) and ECLIPSE50, all these methods have to rely on laboratories which are equipped with precise instruments. In order to solve the problem, our Beta-lactam biosensor is designed to be used on on-site detection in a few hours by users without  special training.</p>
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      <p>Device<br>
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        Beta-Lactam antibiotics have become less effective for the treatment of staphylococcal infections as a result of the bacteria's resistance to Beta-Lactam increases sharply during the past few years. Researches have shown that the resistance is mediated by beta-lactamase (encoded by blaZ) that hydrolyzes penicillin whose transcription is regulated by related regulators (encoded by blaI). The purified repressor(BlaI) of beta-lactamase production has been shown to bind specifically to two regions of dyad symmetry, known as operators, which are located between the divergently transcribed beta-lactamase structural gene(blaZ) and the gene(blaR1) encoding the putative transmembrane sensor protein. </p>
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      <p>The bla operon has been found that is induced by beta-lactam.<br>
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        Hypothesis identified bla as a beta-lactam-sensing operon of beta-lactamase expression, so we designed two devices working in E.coli (DH5α) to build the beta-lactam biosensor.</p>
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    <td><p>Zhangdeyu is the member who is responsible for the electronic device and wiki construction . <br>
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      <p> <br>
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      Tel :86-188-1057-7546 Mailbox:824074989@qq.com</p>
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        Device 1</p>
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      <p>This device will work to detect the concentration of Beta-Lactam in dairy products. At the same time, we designed another two devices to decrease the detection limit.</p>
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        Device 2</p>
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      <p>Result</p>
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      <p><span class="Cap">Amplifier</span></p>
 
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        If we give the biosensor an input signal, we will get an output signal, which, however, may not be strong enough for us to detect. Therefore, we have designed an amplifier, which is based on the high activity of T7 promoter, to increase the intensity of the output signal to a specific magnification. We replaced the sequence of the green fluorescent protein of the sensor with the DNA of T7 RNA polymerase to promote the expression of the downstream DNA. Thus we can get stronger fluorescent intensity as expected. </p>
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        As we all know, if the sample of material which the concentration is high enough to be detected is inserted as the input signal, the sensor will be able to &quot;feel&quot; it and produce an output signal.</p>
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    <td><p> He Keren is a undergraduate student who has been majored in biotechnology. As a student to pursue the most interesting knowledge, I decided to take part in IGEM as a available way to achieve my ideal. Although I am a sophomore student, but I play a important role in our team, because I take charge of innovate of our team. And my knowledge is not enough to my work though, I am keeping studying all the time and I’m confidence . By the way, my best hobby is reading. Apart from this, I like playing basketball very much and my favorite basketball star is Kobe Blyant. And I plan to study in U.S. after my graduate. As we all know, biology is developing all the time, I hope I can know biology more deeply by this time IGEM.</p>
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        However, sometimes, the output is not strong enough for us to detect. To magnify the output signal, we took advantage of the high activity of the T7 promoter. Because the T7 promoter can only be activated by the T7 RNA polymerase, a gene of T7 RNA polymerase and a T7 promoter were inserted at the downstream site of the sensor to get a stronger output. This part is what we call an &quot;amplifier&quot;.<br>
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       <p> Controller<br>
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        Sometimes we need to enhance the output signal to different degrees. In other words, we want to control the magnification. A &quot;controller&quot; is designed to solve this problem. We inserted a lacO operator between the DNA of T7 RNA polymerase and green fluorescent protein, and added a lacI biobrick in the system. When there is low concentration of IPTG, the lacI will close the lacO to inhibit the expression of gfp DNA. When we add IPTG to the sample, the lacI will be combined with IPTG, and the inhibition of the expression of the downstream DNA will be inhibited. Thus we can control the magnification by controlling the concentration of IPTG.</p>
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        Here we introduced a new part which contains lacI and lacO in the system. The gene of lacI is always expressing, which inhibits the expression of lacO. In this case, even if there is an input signal, no egfp will be expressed.</p>
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    <td><p>I<strong> am Mengran Tao, a member of the BIT iGEM team. I am undergraduate  student and major in biotechnology.I participate in the group of the </strong><strong>Chromium ion</strong><strong> biosensor and biological chip. People drink milk every day, so i wish our  biological monitor could help us build the safety of the milk. I have made lots  of friend during the competition, The feeling of working together to achieve  one goal is wonderful. I enjoy it and I am also interested in the synthetic  biology. iGEM gives me a chance to cultivate the team  spirt and enhance my professional knowledge.My hobby is playing basketball and  playing the guitar ,so the NBA and music accompany me in the free time. Anyone  who like NBA or music can make friends with me. </strong><strong> </strong></p></td>
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        If we put IPTG in the environment as an inducer, the lacI protein will combine to the IPTG molecules and thus the inhibition will be ceased. As a result, the lacO will be activated, which will lead to the expression of downstream egfp.</p>
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        With a stable concentration of IPTG, the system will work as expected. When a weak input is given, a weak green fluorescence will be detected, while if the input gets stronger, the intensity of the green fluorescence will increase simultaneously.</p>
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       <p>Qiaorui Xing is an undergraduate student who has been majored in bioengineering since 2010. She played a role of team member in the IGEM contest, and is mainlyresponsible for conducting experiment. Her motivation is to receive more challenge, learn more professional knowledge and to solve practical problem by using biological method. Her motto is that where there is a will, there is a success.So may her succeed. <br>
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        Sometimes we would like to control the magnification. This could be realized by regulating the concentration of IPTG. The higher the concentration of IPTG is, the more lacI will be combined. As a result, the expression of downstream egfp will be enhanced.<br>
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       1511130310@qq.com</p></td>
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        Similarly, we can decrease the magnification by lowering the concentration of IPTG. <br>
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        This is the part what we call a &quot;controller&quot;.</p>
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    <td>MengZijian is a BITundergraduatewhose major is bioengineering.His interest is molecular biology and bioinformatics which is the reason he works on the gene experiment.<br>
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      <p class="Cap">Biochip</p>
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       In addition to the appetite for major, he plays well on the others, such as Chinese chess, drawing, especiallythe ten levels of saxophone. He hopes he can learn more and transform the knowledge into real life through iGEM composition. What he insists on is the potential of biology.21scentury is the generation of biology explosion. The development in the area of subject’s overlapping must change the world. So he gets ready for farther study. What he wants to do is to make progress on biology so that he can contribute to people.<br>
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      <p class="Cap"><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span><span class="Con">Biochip</span></p>
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       E-mail:zijianmeng1@gmail.com<br></td>
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       <p><span class="Cap">Hardware</span><br>
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    <td><img src="https://static.igem.org/mediawiki/2013/a/aa/%E7%94%B0%E9%98%BF%E6%95%8F.jpg" width="97" height="97"></td>
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    <td><p>Tian A’min is a 20-year-old girl who comes from Xianyang City, Shaanxi province, China. Her major is Biomedicine Engineering and she will be a junior when she participate in the iGEM Jamboree. She designs the detection of bichromate in diary products and potable water. She loves to do the biology experiments.<br>
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        Contact information:<br>
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      <p>&nbsp;</p>
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        TEL: 13260233328<br>
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       <p>&nbsp;&nbsp; As the joint of biological and non-biological research, this device aims to detect the fluorescent intensity of eGFP and calculate the concentration of the chemicals based on it. We can then assess the quality of the sample detected.</p>
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        E-mail: 237416567@qq.com</p>
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       <p>The mechanism of this device is simple (figure). </p>
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      <p><img src="https://static.igem.org/mediawiki/2012/4/4a/BIThd1.jpg" width="562" height="218" hspace="20" vspace="20" border="0" align="right"> &nbsp;&nbsp;</p>
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      <p>The exciting light coming from blue LED through 490nm narrowband filter, which only allow 490nm light  to pass through. The filtered light then penetrates our test chip. If GFP exists on our test chip, it would transform the frequency of the excitation light into about 520nm, which is the only frequency of light that can pass through the 517nm narrowband filter. Then our sensor will be able to detect the intensity of the light and calculate the content of GFP, indicating the composition of the tested sample with our mathematical model.</p>
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      <p>&nbsp;&nbsp;The characteristic of our device is that it is really CHEAP. Although it costs less than 300RMB in total and can easily cooperate with our biological products, it is sensitive indeed. Here are the data we got.<br>
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    <td><img src="https://static.igem.org/mediawiki/2013/c/c7/%E9%82%B1%E4%BA%91%E6%B4%81.jpg" width="97" height="97"></td>
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    <td> YunjieQiuis a sophomore majoring in biotechnology at BIT. She is interested in stem cells research and microorganism circuit. Besides iGEM, she is doing a related research at college lababout induced pluripotent stem cells. She wouldlike to learn more about synthetic biology and do a project from the ground with her team in the process of competition. In her space, Qiu enjoys playing badminton and watchingmovies.<br></td>
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    <td><img src="https://static.igem.org/mediawiki/2013/f/f0/%E5%91%A8%E8%8A%AF.jpg" width="97" height="97"></td>
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    <td> Zhou Rui is a student who has been majoring in biotechnology since 2011, When she was a freshman,she joined the lab, mastered the basic experimental technology and lay a solid foundation for the future research. From experiments,she harvests a lot of knowledge which is hard to learn from books and improves the basic experimental skills and creative thinking, fully realizes the rigorous, serious and pragmatic spirit of scientific research. Participating in this project, She will continue this kind of realistic attitude, more seriously perform each experiment.</td>
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    <td><img src="https://static.igem.org/mediawiki/2013/e/ee/%E6%9D%8E%E6%8C%AF%E5%AE%87.jpg" width="97" height="97"></td>
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    <td><p><br>
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      Li Zhenyu is undergraduate student who has been majored in Electronic Packaging since 2011. Although he is not a professional biology student, he still has deep love for the mystery of it. He is good at electronics and mathematics. He is responsible for the hardware part of this project with another one. He has a great passion and confidence.<br>
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Revision as of 01:39, 11 September 2013

iGEM BIT



Team Members

Xin Ma is the president of this team in 2013, who has been majoring in Biomedical Engineering in Beijing Institute of Technology since 2011. Debate, speech, board games, writing and composing are his five favorites. He would like to take advantage of the new discovery related to human health to create a better and more beautiful life for all the mankind. For him, iGEM provides an excellent platform to take a step forward to achieve his ideal;

 
an undergraduate student who has been majoring in biotechnology since 2011.She is a girl who is very outgoing and passionate .In her spare time, she loves reading books, listening to music and dancing. She is the vice-captain of the group. She takes part in the competition to creatively study and apply the relevant knowledge. She is in charge of the biological experiment and deal with some affairs of the group. She believes science and technology can benefit mankind and wants to contribute to the development of mankind.

Biomedical Engineering juniorundergraduates, head of microfluidic chip group, also the contact person of BIT-IGEM team, whose mottois"Life is acontinuous improvementjourney".Lovemusic and sports. Band drummer and also a football team striker. Taking part in this competition in order to improve personal ability by learning from other’s advantage and gain more experience after enjoy the time of this competition. Expecting for this communication and looking forward to meet with every kind of friends in different culture background.

Zhangdeyu is the member who is responsible for the electronic device and wiki construction .
Tel :86-188-1057-7546 Mailbox:824074989@qq.com

 

 

 

 

He Keren is a undergraduate student who has been majored in biotechnology. As a student to pursue the most interesting knowledge, I decided to take part in IGEM as a available way to achieve my ideal. Although I am a sophomore student, but I play a important role in our team, because I take charge of innovate of our team. And my knowledge is not enough to my work though, I am keeping studying all the time and I’m confidence . By the way, my best hobby is reading. Apart from this, I like playing basketball very much and my favorite basketball star is Kobe Blyant. And I plan to study in U.S. after my graduate. As we all know, biology is developing all the time, I hope I can know biology more deeply by this time IGEM.

 

I am Mengran Tao, a member of the BIT iGEM team. I am undergraduate student and major in biotechnology.I participate in the group of the Chromium ion biosensor and biological chip. People drink milk every day, so i wish our biological monitor could help us build the safety of the milk. I have made lots of friend during the competition, The feeling of working together to achieve one goal is wonderful. I enjoy it and I am also interested in the synthetic biology. iGEM gives me a chance to cultivate the team spirt and enhance my professional knowledge.My hobby is playing basketball and playing the guitar ,so the NBA and music accompany me in the free time. Anyone who like NBA or music can make friends with me.

 

Qiaorui Xing is an undergraduate student who has been majored in bioengineering since 2010. She played a role of team member in the IGEM contest, and is mainlyresponsible for conducting experiment. Her motivation is to receive more challenge, learn more professional knowledge and to solve practical problem by using biological method. Her motto is that where there is a will, there is a success.So may her succeed.
1511130310@qq.com
MengZijian is a BITundergraduatewhose major is bioengineering.His interest is molecular biology and bioinformatics which is the reason he works on the gene experiment.
In addition to the appetite for major, he plays well on the others, such as Chinese chess, drawing, especiallythe ten levels of saxophone. He hopes he can learn more and transform the knowledge into real life through iGEM composition. What he insists on is the potential of biology.21scentury is the generation of biology explosion. The development in the area of subject’s overlapping must change the world. So he gets ready for farther study. What he wants to do is to make progress on biology so that he can contribute to people.
E-mail:zijianmeng1@gmail.com

Tian A’min is a 20-year-old girl who comes from Xianyang City, Shaanxi province, China. Her major is Biomedicine Engineering and she will be a junior when she participate in the iGEM Jamboree. She designs the detection of bichromate in diary products and potable water. She loves to do the biology experiments.
Contact information:
TEL: 13260233328
E-mail: 237416567@qq.com
YunjieQiuis a sophomore majoring in biotechnology at BIT. She is interested in stem cells research and microorganism circuit. Besides iGEM, she is doing a related research at college lababout induced pluripotent stem cells. She wouldlike to learn more about synthetic biology and do a project from the ground with her team in the process of competition. In her space, Qiu enjoys playing badminton and watchingmovies.
Zhou Rui is a student who has been majoring in biotechnology since 2011, When she was a freshman,she joined the lab, mastered the basic experimental technology and lay a solid foundation for the future research. From experiments,she harvests a lot of knowledge which is hard to learn from books and improves the basic experimental skills and creative thinking, fully realizes the rigorous, serious and pragmatic spirit of scientific research. Participating in this project, She will continue this kind of realistic attitude, more seriously perform each experiment.


Li Zhenyu is undergraduate student who has been majored in Electronic Packaging since 2011. Although he is not a professional biology student, he still has deep love for the mystery of it. He is good at electronics and mathematics. He is responsible for the hardware part of this project with another one. He has a great passion and confidence.
Email:641437260@qq.com
   












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