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Team:UCSF/Project/Accomplish
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| + | <ul style="list-style-image:url(https://static.igem.org/mediawiki/2013/7/7b/IGEM_Logo_UCSF_Bullet.png);"> | ||
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| + | <li>We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria. </li><br> | ||
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| + | <li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. </li><br> | ||
Revision as of 07:33, 27 September 2013
- We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria.
- We have created a standard for creating "knock-in" cassettes and inserting DNA into the E. coli genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the E. coli genome to mark our conjugation strains.
